Abstract:
:Cells of Yersinia pestis strain EV76 are known to cease growth after a shift from 26 to 37 degrees C in neutral Ca2+-deficient medium; this effect is potentiated by Mg2+. With 2.5 mM Mg2+ and no added Ca2+, restriction was relaxed at elevated pH at which maximum cell yields occurred in the presence of 20 mM exogenous ATP. This ATP-dependent growth was inhibited by Ca2+ or 20 mM Mg2+; the nucleotide was neither transported into the organism nor hydrolyzed extracellularly. With strain EV76, ATP could be replaced by GTP but not other nucleotides, nucleosides, free bases, or pyrophosphate. CTP and UTP also promoted growth of strain KIM, in which limited division also occurred with nucleoside di- and monophosphates. Intracellular V antigen was detected 1 h after temperature shift in Ca2+-deficient medium containing 20 mM Mg2+, a time corresponding to the earliest known events associated with restriction (shutoff of stable RNA synthesis and reduction of adenylate energy charge). Maximum yield of V was obtained 2 h later when cell division ceased; the titer of the antigen remained constant thereafter. The specific activity of V in cells grown with ATP was significantly reduced, especially at elevated pH. These results would be expected if exogenous nucleotides promote growth by sequestering sufficient Mg2+ to prevent restriction of cell division mediated by V antigen.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Zahorchak RJ,Brubaker RRdoi
10.1128/IAI.38.3.953-959.1982subject
Has Abstractpub_date
1982-12-01 00:00:00pages
953-9issue
3eissn
0019-9567issn
1098-5522journal_volume
38pub_type
杂志文章abstract::The ability of oral immunization to interfere with the establishment of infection with Helicobacter felis was examined. Groups of Swiss Webster mice were immunized orally with 250 micrograms of Helicobacter pylori recombinant urease (rUrease) and 10 micrograms of cholera toxin (CT) adjuvant, 1 mg of H. felis sonicate ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1246-1252.1995
更新日期:1995-04-01 00:00:00
abstract::Haemophilus influenzae is a commensal and opportunistic pathogen of the human airways. A number of surface molecules contribute to colonization of the airways by H. influenzae, such as adhesins, including structures found in the lipooligosaccharide (LOS). A human bronchiolar xenograft model was employed to investigate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4661-4668.2002
更新日期:2002-08-01 00:00:00
abstract::The Lyme disease spirochete, Borrelia burgdorferi, exists in a zoonotic cycle involving an arthropod tick and mammalian host. Dissemination of the organism within and between these hosts depends upon the spirochete's ability to traverse through complex tissues. Additionally, the spirochete outruns the host immune cell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01228-12
更新日期:2013-06-01 00:00:00
abstract::Metastatic infection after hematogenous dissemination of Candida species is presumably dependent on the fungus traversing the vascular endothelium. An in vitro model of the earliest events of metastatic Candida infection was developed with whole vascular strips. Freshly obtained porcine blood vessels were secured in a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.374-384.1983
更新日期:1983-10-01 00:00:00
abstract::The 23-membered Esx protein family is involved in the host-pathogen interactions of Mycobacterium tuberculosis. These secreted proteins are among the most immunodominant antigens recognized by the human immune system and have thus been used to develop vaccines and immunodiagnostic tests for tuberculosis (TB). Gene pai...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05344-11
更新日期:2011-10-01 00:00:00
abstract::The lymphogranuloma venereum (LGV) and trachoma biovars of Chlamydia trachomatis exhibit differences in biological properties both in vivo and in vitro. To identify analogous biochemical differences, we studied the molecular charges of chlamydial outer membrane proteins (OMPs) by means of isoelectric focusing and none...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.488-494.1985
更新日期:1985-11-01 00:00:00
abstract::Borrelia burgdorferi encodes a functional homolog of canonical Lon protease termed Lon-2. In addition, B. burgdorferi encodes a second Lon homolog called Lon-1. Recent studies suggest that Lon-1 may function differently from the prototypical Lon protease. However, the function of Lon-1 in B. burgdorferi biology remain...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00951-19
更新日期:2020-05-20 00:00:00
abstract::Haemophilus somnus causes pneumonia, reproductive failure, infectious myocarditis, thrombotic meningoencephalitis, and other diseases in cattle. Although vasculitis is commonly seen as a result of systemic H. somnus infections, the pathogenesis of vascular damage is poorly characterized. In this study, we demonstrated...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1650-1660.2001
更新日期:2001-03-01 00:00:00
abstract::Pools of rat antiserum to Plasmodium berghei had different levels of protective activity as assessed by a passive transfer test. Preincubation of parasite inocula with an effective pool before injection did not significantly enhance protective activity. Removal of the antiserum from preincubated parasite inocula aboli...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1213-1218.1974
更新日期:1974-12-01 00:00:00
abstract::Group B streptococci (GBS) adhere to surface receptors present on epithelial cells; these receptors include fibronectin and laminin. To identify other possible receptors, plasma membranes from A549 cells, a respiratory tract epithelial cell line, were prepared. These plasma membranes were tested in a protein blot anal...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2129-2134.2000
更新日期:2000-04-01 00:00:00
abstract::Neuronal dysfunction can occur in the course of sepsis without meningitis. Sepsis-associated neuronal damage (SAND) was observed in the hippocampus within hours in experimental pneumococcal bacteremia. Intravascular challenge with purified bacterial cell wall recapitulated SAND. SAND persisted in PAFr(-/-) mice but wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00022-06
更新日期:2006-07-01 00:00:00
abstract::Two virulence factors of Listeria monocytogenes, listeriolysin O (LLO) and phosphatidylinositol-specific phospholipase C (PI-PLC), mediate escape of this pathogen from the phagocytic vacuole of macrophages, thereby allowing the bacterium access to the host cell cytosol for growth and spread to neighboring cells. We ch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6639-6646.2005
更新日期:2005-10-01 00:00:00
abstract::The immune response of mice to live, heat-killed, or autoclaved Mycobacterium leprae was investigated. After sensitization with 10(7) organisms in each group, recipient mice were transfused with the sensitized splenocytes 28 days later. A selected number of these mice were infected with 5 X 10(3) M. leprae, and the re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.79-83.1984
更新日期:1984-01-01 00:00:00
abstract::Gram-negative organisms incorporate hydroxy fatty acids into the lipid A moiety of lipopolysaccharide (LPS), and in the case of some members of the family Enterobacteriaceae, hydroxy fatty acids are incorporated exclusively into lipid A. However, a limited number of Bacteroides species have been shown to incorporate s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3753-3760.1994
更新日期:1994-09-01 00:00:00
abstract::Leukosis viruses of seven subgroups were tested for oncogenicity in chickens susceptible to virus infection and to development of lymphoid leukosis (LL) tumors. All subgroup A viruses and the subgroup B virus tested produced a high incidence of LL and other related neoplasms. Viruses of subgroup C and RAV-61 of subgro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.2.423-428.1977
更新日期:1977-02-01 00:00:00
abstract::Pathogenicity of Rickettsia tsutsugamushi for laboratory mice is known to be influenced by at least three factors: (i) route of inoculation, (ii) antigenic strain, and (iii) natural resistance of the host. By using Karp, Gilliam, and Kato strains of R. tsutsugamushi, we examined the effect of these three pathogenicity...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1476-1482.1989
更新日期:1989-05-01 00:00:00
abstract::Antibiotic-resistant recombinants obtained from mating antibiotic-resistant Escherichia coli with virulent, antibiotic-sensitive Salmonella typhimurium are generally avirulent. After 32 consecutive transfers, two of four avirulent recombinants regained their virulence without loss of episome-mediated resistance. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.4.516-518.1970
更新日期:1970-10-01 00:00:00
abstract::Splenic lymphocytes from cytomegalovirus-infected mice lost their in vitro proliferative responses to cytomegalovirus antigen within 3 h after in vivo treatment with antilymphocyte globulin and prednisolone. The response was inhibited when the agents were administered separately or together, and inhibition persisted t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::Murine embryo fibroblasts (MEF) were found to secrete tumor necrosis factor (TNF) in response to stimulation with endotoxin. Endotoxin-induced TNF production by MEF was inhibited by cycloheximide. However, reversal of the effect of this inhibitor on protein synthesis results in TNF being secreted in amounts equivalent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.1630-1635.1993
更新日期:1993-05-01 00:00:00
abstract::Helicobacter pylori colonizes the gastric epithelium of at least 50% of the world's human population, playing a causative role in the development of chronic gastritis, peptic ulcers, and gastric adenocarcinoma. Current evidence indicates that H. pylori can invade epithelial cells in the gastric mucosa. However, relati...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01085-06
更新日期:2006-12-01 00:00:00
abstract::Subcutaneous infection chambers in rabbits were infected with a strain of Staphylococcus aureus isolated from a patient with toxic shock syndrome. Estrogens (mestranol and 17-beta-estradiol) protected male rabbits and prolonged survival. Neither androgens (testosterone and dihydrotestosterone) nor progesterone affecte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.331-333.1986
更新日期:1986-04-01 00:00:00
abstract::A cysteine-containing peptide motif, EWSPCSVTCG, is found highly conserved in the circumsporozoite protein (CSP) and the thrombospondin-related anonymous protein (TRAP) of all the Plasmodium species analyzed so far and has been shown to be crucially involved in the sporozoite invasion of hepatocytes. We have recently ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3232-3241.1998
更新日期:1998-07-01 00:00:00
abstract::Individuals with sickle cell anemia are subject to serious infections caused by a number of bacteria, including Salmonella species and Staphylococcus aureus. It has been suggested that in sickle cell anemia, extensive erythrophagocytosis by macrophages may interfere with their antibacterial function and thereby predis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.3.917-923.1983
更新日期:1983-06-01 00:00:00
abstract::Oligosaccharides (OSs) related to the pneumococcal type 14 capsular polysaccharide (Pn14PS) were studied for their ability to inhibit the binding between anti-PS14 antisera and native PS14. A synthetic tetrasaccharide corresponding to the repeating unit of the Pn14PS, a hexasaccharide mimic, and an octasaccharide frag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.9.5107-5114.2002
更新日期:2002-09-01 00:00:00
abstract::Production of group A streptococcal pyrogenic exotoxins (SPE) type A and C was transferred from toxigenic streptococcal strains to nontoxigenic strains by lysogeny. Lysogens were tested for SPE with Ouchterlony immunodiffusion on Todd-Hewitt agar plates; toxin diffusing from isolated colonies reacted with specific hyp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.28.1.254-257.1980
更新日期:1980-04-01 00:00:00
abstract::Salmonella enterica serovar Typhimurium causes self-limiting gastroenteritis in humans and a typhoid-like disease in mice that serves as a model for typhoid infections in humans. A critical step in Salmonella pathogenesis is the invasion of enterocytes and M cells of the small intestine via expression of a type III se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2002-2013.2004
更新日期:2004-04-01 00:00:00
abstract::A zone of extracellular digestion of the mucin layer around Candida albicans blastoconidia was observed by transmission electron microscopy in the jejunum of mice inoculated intragastrically (G. T. Cole, K. R. Seshan, L. M. Pope, and R. J. Yancey, J. Med. Vet. Mycol. 26:173-185, 1988). This observation prompted the hy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4514-4519.1996
更新日期:1996-11-01 00:00:00
abstract::Phytohemagglutinin is able to trigger interferon synthesis in spleen cell cultures from nude (nu/nu) mice as effectively as in splenic cell cultures from haired, control (nu/+), thymus-bearing mice. A minor theta-bearing cell population present in the spleen of nude mice appears essential to phytohemagglutinin interfe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.3.966-972.1978
更新日期:1978-09-01 00:00:00
abstract::Acute pyelonephritis, a complication of Escherichia coli bacteriuria, must represent a bacterial invasion through the kidney epithelium. To study this process, we overlaid bacterial suspensions onto monolayers of cultured human kidney proximal tubular epithelial cells and measured cytotoxicity by release of lactate de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1281-1289.1990
更新日期:1990-05-01 00:00:00
abstract::We examined the reactivities of Clostridium butyricum neurotoxin to nine monoclonal antibodies against Clostridium botulinum type E neurotoxin which recognize the light chain or the amino-terminal half (H-1 fragment) or the carboxyl-terminal half (H-2 fragment) of the heavy chain of botulinum neurotoxin. Butyricum neu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.1.457-459.1991
更新日期:1991-01-01 00:00:00