Abstract:
:Group B streptococci (GBS) adhere to surface receptors present on epithelial cells; these receptors include fibronectin and laminin. To identify other possible receptors, plasma membranes from A549 cells, a respiratory tract epithelial cell line, were prepared. These plasma membranes were tested in a protein blot analysis using radiolabeled GBS as a probe. GBS adhered to two species, with molecular masses of 50 kDa (p50) and 57 kDa (p57). We concluded that p50 and p57 correspond to two forms of cytokeratin 8 (CK8) on the basis of the following results: (i) protein blot results demonstrated that p50 and p57 exactly comigrated with two forms of CK8 after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE); (ii) p50 and p57 exactly comigrated with CK8 after separation by two-dimensional PAGE; (iii) CK8 in solution bound to GBS, as demonstrated by immunoblot analysis of proteins from A549 lysates that bound to GBS in a liquid-phase assay; and (iv) radiolabeled GBS bound to A549 lysate-derived CK8 that had been captured in anti-CK8-coated microtiter wells. CK8 bound to COH1-13, an acapsular mutant of COH1, demonstrating that adherence is not mediated by capsular polysaccharide. Trypsin-treated GBS did not bind to CK8, indicating that adherence is mediated via a protein on the surface of GBS. Soluble CK8 bound to six of six GBS strains tested. Soluble CK8 also bound to Staphylococcus aureus, Lactococcus lactis, Enterococcus faecalis, and Streptococcus pyogenes. We hypothesize that adherence of GBS to cytokeratin may be important for maintenance of colonization at sites of keratinized epithelium, such as the vagina, or for adherence of these bacteria to damaged epithelial cells at other sites.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Tamura GS,Nittayajarn Adoi
10.1128/iai.68.4.2129-2134.2000keywords:
subject
Has Abstractpub_date
2000-04-01 00:00:00pages
2129-34issue
4eissn
0019-9567issn
1098-5522journal_volume
68pub_type
杂志文章abstract::The mammalian host response to infection includes the production and secretion of antimicrobial peptides from phagocytes and epithelial cells. Protegrins, a group of broadly microbicidal peptides isolated originally from porcine neutrophil lysates, were found to be stored as inactive proforms in porcine neutrophil gra...
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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pub_type: 杂志文章
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更新日期:1999-11-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1993-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.751-755.1988
更新日期:1988-04-01 00:00:00
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更新日期:2004-06-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1986-06-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1993-09-01 00:00:00
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pub_type: 杂志文章,收录出版
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更新日期:2012-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1999-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00