Abstract:
:Ehrlichia risticii incubated with mouse peritoneal macrophages elicited with thioglycolate broth survived and replicated, thereby allowing examination of the effects of several immunopotentiating agents. Treatment of the macrophages with recombinant murine gamma interferon (rMuIFN-gamma) in vitro at 1 day before or 3 h after infection made the macrophages resistant to infection with E. risticii, and macrophages treated with rMuIFN-gamma at 1 to 3 days after infection developed the capacity to eradicate intracellular E. risticii. Similar effects were seen with macrophages treated with the Ca2+ ionophore A23187 before or after E. risticii infection in vitro. Concanavalin A treatment before or 3 h after infection caused the macrophages to become resistant to infection with E. risticii but could confer neither ehrlichiacidal nor ehrlichiastic activity to them once infection had been established for more than 1 day. Bacterial products such as lipopolysaccharide and muramyl dipeptide were less or not at all effective, respectively, in conferring antiehrlichial activity to macrophages. Finally, protein kinase C activator, phorbol myristate acetate, and recombinant tumor necrosis factor did not induce any antiehrlichial activity in macrophages when the macrophages were treated either before or after infection.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Park J,Rikihisa Ydoi
10.1128/IAI.59.10.3418-3423.1991subject
Has Abstractpub_date
1991-10-01 00:00:00pages
3418-23issue
10eissn
0019-9567issn
1098-5522journal_volume
59pub_type
杂志文章abstract::A progressive increase in infections with multiresistant Enterococcus faecium has been reported, especially in cancer patients and neutropenic patients. Despite its increasing importance as a nosocomial pathogen, knowledge of the pathogenesis of E. faecium infections is highly limited. In this study, we investigated t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00863-08
更新日期:2009-01-01 00:00:00
abstract::A 30-amino-acid peptide corresponding to variable domain IV (VD IV) of the major outer membrane protein of Chlamydia trachomatis serovar E was conjugated to keyhole limpet hemocyanin (KLH) and used to immunize mice. The resulting antisera (anti-KLH-VD IV sera) recognized all 15 serovars of C. trachomatis when assayed ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3428-3432.1992
更新日期:1992-08-01 00:00:00
abstract::Serum was necessary for optimal phagocytosis of Aspergillus fumigatus spores by human leukocytes, and its opsonic capacity was greatly diminished by heat inactivation (56 C, 30 min). A germination assay, described in this report, was developed to study the fate of phagocytized spores. After incubation for 3 hr with no...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.1.4.345-350.1970
更新日期:1970-04-01 00:00:00
abstract::Escherichia coli D2 (serotype 07:H-) that was isolated from a child with diarrhea hybridized with an F1845 DNA probe used to detect diffuse adherence. Strain D2 adhered to tissue culture cells (HeLa and HEp-2 cells) in a clustered pattern but did not autoagglutinate on the cell surface and induced the elongation of mi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.2917-2929.1994
更新日期:1994-07-01 00:00:00
abstract::Cell-mediated immunity to herpes simplex virus type 1 (HSV-1) was assessed by a lymphocytotoxicity 51-Cr-release microassay procedure, using the MA-160 human prostatic adenoma cell line chronically infected with HSV-1 and its parent cell line as control. The specific immune release mean plus or minus standard deviatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.1.76-80.1975
更新日期:1975-07-01 00:00:00
abstract::Cells of Candida albicans strain WO-1 and related strains switch frequently and reversibly between a white-colony-forming unit (white phase) and a gray-colony-forming unit (opaque phase). Cells in the budding white phase exhibit the usual smooth round phenotype observed in other C. albicans strains, but cells in the b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.2.458-467.1989
更新日期:1989-02-01 00:00:00
abstract::Pertussis toxin is secreted from Bordetella pertussis with the assistance of the Ptl transport system, a member of the type IV family of macromolecular transporters. The S1 subunit and the B oligomer combine to form the holotoxin prior to export from the bacterial cell, although the site of assembly is not known. To b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1193-1201.2002
更新日期:2002-03-01 00:00:00
abstract::Treatment with nontoxic monophosphoryl lipid A (MPL), which was derived from a polysaccharide-deficient, heptoseless Re mutant of Salmonella typhimurium, was found to inactivate suppressor T-cell activity, as evidenced by a decrease in the degree of low-dose immunological paralysis expressed and an increase in the mag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1076-1083.1988
更新日期:1988-05-01 00:00:00
abstract::G protein-coupled receptor kinase 5 (GRK5) is a serine/threonine kinase previously shown to mediate polymicrobial sepsis-induced inflammation. The goal of the present study was to examine the role of GRK5 in monomicrobial pulmonary infection by using an intratracheal Escherichia coli infection model of pneumonia. We u...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00051-16
更新日期:2016-04-22 00:00:00
abstract::Entamoeba histolytica virulence is related to a number of amebic components (lectins, cysteine proteinases, and amebapore) and host factors, such as intestinal bacterial flora. Trophozoites are selective in their interactions with bacteria, and the parasite recognition of glycoconjugates plays an important role in ame...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.5.2096-2102.1999
更新日期:1999-05-01 00:00:00
abstract::We investigated the effect of an extract from a helminth (Ascaris suum) in zymosan-induced arthritis (ZYA) or collagen-induced arthritis (CIA). Rats and mice, respectively, received 1 mg and 0.1 mg zymosan intra-articularly (i.a.). Test groups received an A. suum extract either per os (p.o.) or intraperitoneally (i.p....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01085-07
更新日期:2008-06-01 00:00:00
abstract::Arthritis is a frequent and major complication of infection with Borrelia burgdorferi sensu stricto. The antigens responsible for the induction of arthritis are unknown. Here we provide direct evidence that a major surface protein, outer surface protein A (OspA), can induce arthritis. Hamsters were vaccinated with 30,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.658-663.2000
更新日期:2000-02-01 00:00:00
abstract::Leptospira interrogans is an important mammalian pathogen. Transmission from an environmental source requires adaptations to a range of new environmental conditions in the organs and tissues of the infected host. Since many pathogenic bacteria utilize temperature to discern their environment and regulate the synthesis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.400-404.2001
更新日期:2001-01-01 00:00:00
abstract::Reinfection of immune mice with Leishmania major elicits a secondary gamma interferon (IFN-gamma) response to which specific CD8+ T cells are essential. We have shown previously that specific CD8+ T cells from reinfected immune mice release substantially higher levels of IFN-gamma, a cytokine essential for the efficie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.6.2575-2581.1994
更新日期:1994-06-01 00:00:00
abstract::We evaluated sexual recombination in the apicomplexan parasite Theileria parva using genome-wide marker analysis of haploid sporozoite populations obtained from infected Rhipicephalus appendiculatus ticks. Analysis of 231 parasite clones derived by in vitro infection of bovine lymphocytes revealed 48 distinct combinat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00472-06
更新日期:2006-10-01 00:00:00
abstract::The hallmarks of pulmonary Mycobacterium tuberculosis infection are lung granulomas. These organized structures are composed of host immune cells whose purpose is to contain or clear infection, creating a complex hub of immune and bacterial cell activity, as well as limiting pathology in the lungs. Yet, given cellular...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00426-18
更新日期:2018-08-22 00:00:00
abstract::Increased incidence of bovine tuberculosis (TB) in the United Kingdom caused by infection with Mycobacterium bovis is a cause of considerable economic loss to farmers and the government. The Eurasian badger (Meles meles) represents a wildlife source of recurrent M. bovis infections of cattle in the United Kingdom, and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00052-08
更新日期:2008-08-01 00:00:00
abstract::PspC was found to bind human complement factor H (FH) by Western blot analysis of D39 (pspC(+)) and an isogenic mutant TRE108 (pspC). We confirmed that PspA does not bind FH, while purified PspC binds FH very strongly. The binding of FH to exponentially growing pneumococci varied among different isolates when analyzed...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3435-3437.2001
更新日期:2001-05-01 00:00:00
abstract::The response of ferrets after intranasal inoculation of inactivated A/Hong Kong/68 (H3N2) influenza virus vaccines is reported. Normal ferrets given either saline vaccine in drops or freeze-dried vaccine in an aerosol intranasally did not produce detectable serum or nasal hemagglutination inhibiting antibody and were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.6.985-990.1974
更新日期:1974-06-01 00:00:00
abstract::Group A streptococcal cell walls inhibited phagocytosis of polystyrene latex particles by rat peritoneal macrophages in vitro. This inhibition was not accompanied by a measurable loss of cell viability. Group D streptococcal cell walls were relatively ineffective in altering phagocytosis. The effectiveness of group A ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.258-261.1978
更新日期:1978-04-01 00:00:00
abstract::The production of type 1 fimbriae in Salmonella enterica serovar Typhimurium is controlled, in part, by three proteins, FimZ, FimY, and FimW. Amino acid sequence analysis indicates that FimZ belongs to the family of bacterial response regulators of two-component systems. In these studies, we have demonstrated that int...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00795-13
更新日期:2013-12-01 00:00:00
abstract::Altogether 171 serum specimens from 58 patients with heterophil antibody-positive infectious monomucleosis were studied for antibody response to Epstein-Barr virus (EBV). The sera were tested for fluorescent immunoglobulin G (IgG) and IgM gel-precipitating (GP) and complement-fixing (CF) antibodies to EBV. All 58 pati...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.42-51.1975
更新日期:1975-01-01 00:00:00
abstract::D-Amino acid oxidase (DAO) is a hydrogen peroxide-generating enzyme that uses a D-amino acid as a substrate. We hypothesized that DAO may protect against bacterial infection, because hydrogen peroxide is one of the most important molecules in the antibacterial defense systems in mammals. We show here that DAO suppress...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06214-11
更新日期:2012-04-01 00:00:00
abstract::Whole cells, morphological components, and various extracts of Candida albicans were tested for toxicity by methods involving biological activities ordinarily used to characterize bacterial endotoxin. Fungal preparations exerted several of these activities, but only at much higher dose levels than those required for b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.4.616-627.1972
更新日期:1972-10-01 00:00:00
abstract::Actinomyces naeslundii and Streptococcus gordonii, oral bacteria that possess Gal/GalNAc- and sialic acid-reactive lectins, respectively, were adherent to immobilized secretory immunoglobulin A (IgA) and two IgA1 myeloma proteins but not to two IgA2 myeloma proteins. Apparently, O-linked oligosaccharides at the hinge ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5421-5424.1996
更新日期:1996-12-01 00:00:00
abstract::To determine the ultrastructural distribution and immunological accessibility of proteins II (P.IIs) on the surfaces of whole gonococci, anti-P.II gold probes were developed and used in electron microscopic studies of viable P.II-expressing variants of Neisseria gonorrhoeae FA1090. Anti-P.II probes clearly marked the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.1003-1006.1988
更新日期:1988-04-01 00:00:00
abstract::Chemiluminescence of human alveolar macrophages (AM) was evaluated in vitro. Unstimulated AM generated chemiluminescence that remained constant during incubation. Addition of heat-killed Staphylococcus aureus 502A (HKB) or a chemical agent, phorbol myristate acetate, produced high rates of chemiluminescence that were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.1.117-120.1977
更新日期:1977-07-01 00:00:00
abstract::Hypoferremia, the reduction of plasma transferrin iron levels during infection, has been shown to control Neisseria meningitidis infection in mice. The exact nature of the mechanism that regulates this response has been obscure. We have previously shown that hypoferremia does not result from an accelerated removal of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.2.320-325.1984
更新日期:1984-05-01 00:00:00
abstract::Two murine monoclonal antibodies (immunoglobulin M) evoked against human kidney tissue were examined for cross-reactivity with group A streptococci. A glomerulus-specific antibody, PMII.40.H.2, cross-reacted in an enzyme-linked immunosorbent assay with purified pepsin-extracted M proteins from type 6 and 12 streptococ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2416-2419.1987
更新日期:1987-10-01 00:00:00
abstract::Intracellular bacterial infections affect all vertebrates. Cultured fish are particularly vulnerable because no effective protection measures have been established since such infections emerged approximately 50 years ago. As in other vertebrates, the induction of cell-mediated immunity (CMI) plays an important role in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00459-19
更新日期:2019-10-18 00:00:00