Abstract:
:Entamoeba histolytica virulence is related to a number of amebic components (lectins, cysteine proteinases, and amebapore) and host factors, such as intestinal bacterial flora. Trophozoites are selective in their interactions with bacteria, and the parasite recognition of glycoconjugates plays an important role in amebic virulence. Long-term monoxenic cultivation of pathogenic E. histolytica trophozoites, strains HK-9 or HM-1:IMSS, with Escherichia coli serotype O55, which binds strongly to the Gal/GalNAc amebic lectin, markedly reduced the trophozoites' adherence and cytopathic activity on cell monolayers of baby hamster kidney (BHK) cells. Specific probes prepared from E. histolytica lectin genes as well as antibodies directed against the light (35-kDa) and heavy (170-kDa) subunits of the Gal/GalNAc lectin revealed a decrease in the transcription and expression of the light subunit in trophozoites grown monoxenically with E. coli O55. This effect was not observed when E. histolytica was grown with E. coli 346, a mannose-binding type I pilated bacteria. Our results suggest that the light subunit of the amebic lectin is involved in the modulation of parasite adherence and cytopathic activity.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Padilla-Vaca F,Ankri S,Bracha R,Koole LA,Mirelman Ddoi
10.1128/IAI.67.5.2096-2102.1999keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
2096-102issue
5eissn
0019-9567issn
1098-5522journal_volume
67pub_type
杂志文章abstract::Vero cytotoxin-producing Escherichia coli (VTEC) strains have been associated with sporadic cases and outbreaks of hemorrhagic colitis and with the hemolytic uremic syndrome in humans. Since adherence of enteric pathogens to epithelial surfaces is often a prerequisite for the subsequent delivery of bacterial enterotox...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.756-761.1988
更新日期:1988-04-01 00:00:00
abstract::Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chroma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.982-986.1976
更新日期:1976-03-01 00:00:00
abstract::The importance of CD4+ cells and gamma interferon (IFN-gamma) in the resolution of established Cryptosporidium parvum infection was investigated with a murine model of cryptosporidiosis in severe combined immunodeficient (SCID) mice. C. parvum-infected SCID mice were reconstituted with spleen cells from immunocompeten...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3928-3932.1993
更新日期:1993-09-01 00:00:00
abstract::Previous data from our laboratory suggest that the transitional epithelium of the urinary bladder secretes and binds to its surface a glycosaminoglycan. The presence of this substance at the bladder surface markedly reduces the ability of microorganisms to adhere to the mucosa. Furthermore, this glycosaminoglycan can ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.3.876-881.1980
更新日期:1980-03-01 00:00:00
abstract::Antibody rises to various virus subcomponents were measured by enzyme-linked immunosorbent assay in the paired sera of volunteers experimentally infected with human coronavirus 229E group viruses. Most of the antibody made during infection was directed against the virus surface projections, with only small amounts of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.845-849.1981
更新日期:1981-03-01 00:00:00
abstract::Oligosaccharide (OS)-protein conjugates are promising candidate vaccines against encapsulated bacteria, such as Haemophilus influenzae, Neisseria meningitidis, and Streptococcus pneumoniae. Although the effects of several variables such as OS chain length and protein carrier have been studied, little is known about th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.6915-6920.2003
更新日期:2003-12-01 00:00:00
abstract::Staphylococcus aureus Newman with an insertion mutation in clfB, the gene encoding clumping factor B, only marginally decreased infection rate (P>0.05) in rats with experimental endocarditis. In contrast, clfB complementation on a multicopy plasmid significantly increased infectivity (P<0.05) over the deleted mutants....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.5443-5446.2000
更新日期:2000-09-01 00:00:00
abstract::The bacterial pathogen Listeria monocytogenes causes foodborne systemic disease in pregnant women, which can lead to preterm labor, stillbirth, or severe neonatal disease. Colonization of the maternal decidua appears to be an initial step in the maternal component of the disease as well as bacterial transmission to th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00153-17
更新日期:2017-07-19 00:00:00
abstract::To assess the relationship between the within-host diversity of malaria infections and the susceptibility of the host to subsequent infection, we genotyped 60 children's successive infections from birth through 3 years of life. MSP-1 Block2 genotypes were used to estimate the complexity of infection (COI). Malaria tra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.12.7783-7792.2001
更新日期:2001-12-01 00:00:00
abstract::Amebic erythrophagocytosis is characteristic of invasive amebiasis, and mutants deficient in erythrocyte ingestion are avirulent. We sought to understand the molecular mechanisms underlying erythrocyte phagocytosis by Entamoeba histolytica. Following adherence to amebae, erythrocytes became round and crenulated, and p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3422-3430.2005
更新日期:2005-06-01 00:00:00
abstract::In this study, we investigated the cell wall-anchored fibronectin-binding proteins SpsD and SpsL from the canine commensal and pathogen Staphylococcus pseudintermedius for their role in promoting bacterial invasion of canine progenitor epidermal keratinocytes (CPEK). Invasion was examined by the gentamicin protection ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00542-15
更新日期:2015-10-01 00:00:00
abstract::Experimental infection of mice with a virulent strain of Mycobacterium avium leads to a slowly progressive disease, which we have previously shown culminates in loss of gamma interferon (IFN-gamma) production by T lymphocytes and death of the animals approximately 40 weeks after infection. Here we investigated the cha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.1.27-35.2002
更新日期:2002-01-01 00:00:00
abstract::Genomic DNA fingerprint analysis was performed on 39 Staphylococcus aureus and 28 Enterococcus faecalis endophthalmitis isolates collected from multiple clinical centers. Among 21 S. aureus genomic DNA fingerprint patterns identified, five clonotypes were recovered from multiple unrelated patients and accounted for 58...
journal_title:Infection and immunity
pub_type: 杂志文章,多中心研究
doi:10.1128/IAI.66.1.356-360.1998
更新日期:1998-01-01 00:00:00
abstract::Mice were vaccinated with plasmid DNA (pDNA) encoding antigen 85A (Ag85A), Ag85B, or PstS-3 from Mycobacterium tuberculosis either in saline or formulated for intramuscular injections in VC1052:DPyPE (aminopropyl-dimethyl-myristoleyloxy-propanaminium bromide-diphytanoylphosphatidyl-ethanolamine) (Vaxfectin; Vical, Inc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3681-3688.2002
更新日期:2002-07-01 00:00:00
abstract::Macrophages can undergo apoptosis after infection with Mycobacterium tuberculosis. This macrophage response deprives the bacillus of its niche cell and supports the host response through better antigen presentation. The intracellular pathways of apoptosis that elaborate this macrophage response are not well understood...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01107-06
更新日期:2007-04-01 00:00:00
abstract::Studies on the innate immune response against microbial infections in Drosophila melanogaster involve mutant strains and their reference strains that act as experimental controls. We used five standard D. melanogaster laboratory reference strains (Oregon R, w1118, Canton-S, Cinnabar Brown, and Yellow White [YW]) and i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02318-14
更新日期:2014-10-01 00:00:00
abstract::The genes for outer membrane protein P2 of four nontypeable Haemophilus influenzae strains were cloned and sequenced. The derived amino acid sequences were compared with the outer membrane protein P2 sequence from H. influenzae type b MinnA and the sequences of P2 from three additional nontypeable H. influenzae strain...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.6.2639-2643.1994
更新日期:1994-06-01 00:00:00
abstract::Candida albicans is a common opportunistic fungal pathogen and is the leading cause of invasive fungal diseases in immunocompromised individuals. The induction of cell-mediated immunity to C. albicans is one of the main tasks of cells of the innate immune system, and in vitro evidence suggests that integrin α(M)β₂ (CR...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00650-10
更新日期:2011-04-01 00:00:00
abstract::Exposure of mycobacterial growth inhibitory factor (MycoIF) to trypsin, chymotrypsin, or neuraminidase decrease its ability to produce intracellular inhibition of mycobacterial growth within macrophages, suggesting that MycoIF was a glycoprotein. MycoIF was unaffected by deoxyribonuclease or ribonuclease. Supernatant ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.4.841-850.1975
更新日期:1975-10-01 00:00:00
abstract::Salmonella enterica serovar Typhimurium causes self-limiting gastroenteritis in humans and a typhoid-like disease in mice that serves as a model for typhoid infections in humans. A critical step in Salmonella pathogenesis is the invasion of enterocytes and M cells of the small intestine via expression of a type III se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2002-2013.2004
更新日期:2004-04-01 00:00:00
abstract::A defect in lymphocyte function could be responsible for persistent infection by the equine infectious anemia virus. The number of lymphocytes bearing surface immunoglobulin, as detected by immunofluorescence, and lymphocyte response to mitogens were the same in uninfected and equine infectious anemia-infected animals...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.4.679-682.1973
更新日期:1973-10-01 00:00:00
abstract::Vaccine adjuvants exert critical and unique influences on the quality of immune responses induced during active immunizations. We investigated the mechanisms of action of immunological adjuvants in terms of their requirements for cytokine-mediated pathways for adjuvanticity. Antibody responses potentiated by several a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5329-5336.1998
更新日期:1998-11-01 00:00:00
abstract::A set of four monoclonal antibodies was produced against a highly purified pepsin extract of type 5 streptococcal M protein. Three of the four antibodies cross-reacted with purified M proteins from heterologous serotypes and opsonized the respective heterologous organisms. Our studies suggest that monoclonal antibodie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.1.267-269.1984
更新日期:1984-10-01 00:00:00
abstract::Assembly of adherent biofilms is the key mechanism involved in Staphylococcus epidermidis virulence during device-associated infections. Aside from polysaccharide intercellular adhesin (PIA), the accumulation-associated protein Aap and the extracellular matrix binding protein Embp act as intercellular adhesins, mediat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01142-10
更新日期:2011-06-01 00:00:00
abstract::This study investigates the role of CD4+ T cells in host defense against cholera enterotoxin-induced diarrhea. Antitoxin immunoglobulin A formation and gut protection against cholera toxin (CT) following oral immunizations with CT were evaluated in normal mice and mice that had been depleted of CD4+ T cells by in vivo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3630-3638.1991
更新日期:1991-10-01 00:00:00
abstract::Borrelia burgdorferi, the agent of Lyme disease, is transmitted by ticks. During transmission from the tick to the host, spirochetes are delivered with tick saliva, which contains the salivary protein Salp15. Salp15 has been shown to protect spirochetes against B. burgdorferi-specific antibodies. We now show that Salp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00232-08
更新日期:2008-07-01 00:00:00
abstract::We undertook a morphological and immunochemical comparison of purified outer membrane antigens from oral and nonoral isolates of Bacteroides asaccharolyticus. Electron micrographs of thin sections of whole bacteria revealed a compact, electron-dense capsule external to the outer membrane of oral strains. A loose, web-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.589-596.1980
更新日期:1980-02-01 00:00:00
abstract::The mechanism by which capsular polysaccharides inhibit phagocytosis is not clearly understood. We investigated the association between a negative surface charge and inhibition of phagocytosis by the capsular polysaccharide of Cryptococcus neoformans. A two-polymer aqueous-phase system containing phosphate ions was us...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Ninety-six Aeromonas hydrophila isolates were tested for cytotoxin and hemolysin production. Sixty-six (69%) of the isolates were both cytotoxic and hemolytic, whereas the rest produced neither cytotoxin nor hemolysin. No evidence of a separate cytotonic activity could be found in any of the isolates. Cytotoxin activi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.3.829-837.1979
更新日期:1979-03-01 00:00:00
abstract::The adenylate cyclase-hemolysin of Bordetella pertussis consists of a cell-invasive N-terminal adenylate cyclase domain linked to a C-terminal RTX hemolysin containing extensive glycine-rich repeats. The toxin is an essential virulence factor required in the initial stages of infection. Adenylate cyclase-hemolysin was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3309-3315.1995
更新日期:1995-09-01 00:00:00