Abstract:
:In this study, we investigated the cell wall-anchored fibronectin-binding proteins SpsD and SpsL from the canine commensal and pathogen Staphylococcus pseudintermedius for their role in promoting bacterial invasion of canine progenitor epidermal keratinocytes (CPEK). Invasion was examined by the gentamicin protection assay and fluorescence microscopy. An ΔspsD ΔspsL mutant of strain ED99 had a dramatically reduced capacity to invade CPEK monolayers, while no difference in the invasion level was observed with single mutants. Lactococcus lactis transformed with plasmids expressing SpsD and SpsL promoted invasion, showing that both proteins are important. Soluble fibronectin was required for invasion, and an RGD-containing peptide or antibodies recognizing the integrin α5β1 markedly reduced invasion, suggesting an important role for the integrin in this process. Src kinase inhibitors effectively blocked internalization, suggesting a functional role for the kinase in invasion. In order to identify the minimal fibronectin-binding region of SpsD and SpsL involved in the internalization process, recombinant fragments of both proteins were produced. The SpsD520-846 and SpsL538-823 regions harboring the major fibronectin-binding sites inhibited S. pseudintermedius internalization. Finally, the effects of staphylococcal invasion on the integrity of different cell lines were examined. Because SpsD and SpsL are critical factors for adhesion and invasion, blocking these processes could provide a strategy for future approaches to treating infections.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Pietrocola G,Gianotti V,Richards A,Nobile G,Geoghegan JA,Rindi S,Monk IR,Bordt AS,Foster TJ,Fitzgerald JR,Speziale Pdoi
10.1128/IAI.00542-15subject
Has Abstractpub_date
2015-10-01 00:00:00pages
4093-102issue
10eissn
0019-9567issn
1098-5522pii
IAI.00542-15journal_volume
83pub_type
杂志文章abstract::Leishmaniae are protozoans which, depending upon both the host and parasite species, can cause either a healing or nonhealing infection. While C57BL/10 mice are able to heal following infection with Leishmania major, they fail to heal following infection with Leishmania amazonensis. In order to address the role of Th1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.7.2952-2959.1993
更新日期:1993-07-01 00:00:00
abstract::A substance with potent decomplementation activity was isolated from staphylococcal culture supernatants by polyethylene glycol precipitation, DEAE-ion-exchange and Sephacryl chromatography, and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified substance exhibited all the characterist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.47-51.1985
更新日期:1985-01-01 00:00:00
abstract::Raising the magnesium level from 0.4 to 1.5 mM in a medium containing only amino acids (2.08%), salts, and vitamins increased enterotoxin B production by Staphylococcus aureus S-6 by about 80%. The level of phosphates in the medium was lowered to one-tenth (to 2.87 mM) the original amount without adversely affecting g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.158-160.1978
更新日期:1978-04-01 00:00:00
abstract::Streptococcus mutans, a major pathogen of dental caries, is considered one of the causative agents of infective endocarditis (IE). Recently, bacterial DNA encoding 120-kDa cell surface collagen-binding proteins (CBPs) has frequently been detected from S. mutans-positive IE patients. In addition, some of the CBP-positi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00401-17
更新日期:2017-11-17 00:00:00
abstract::Shiga toxigenic Escherichia coli (STEC) strains are a diverse group of organisms capable of causing severe gastrointestinal disease in humans. Within the STEC family, eae-positive STEC strains, particularly those belonging to serogroups O157 and O111, appear to have greater virulence for humans. However, in spite of b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.5930-5937.1999
更新日期:1999-11-01 00:00:00
abstract::Antiserum and purified immunoglobulin directed against Mycoplasma pneumoniae membrane protein P1 were examined for their influence on mycoplasma viability, metabolism, and cytadsorption. Anti-P1 immunoglobulin inhibited adherence of M. pneumoniae to hamster tracheal rings in vitro by up to 80% and inhibited hemadsorpt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.3.1180-1186.1983
更新日期:1983-03-01 00:00:00
abstract::Female C57BL/6J mice were infected with Trypanosoma cruzi and subsequently given macrophages or lymphocytes from syngeneic donors which had recovered from the acute infection. Mice which received immune peritoneal macrophages, splenic lymphocytes, or lymph node lymphocytes developed lower mean parasitemias and cumulat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.3.838-843.1979
更新日期:1979-09-01 00:00:00
abstract::In previous work (Jones, Infect, Immun. 30:78-89, 1980) a major cytoplasmic antigen of Candida albicans was identified. In both humans and experimental animals, this antigen is released from C. albicans during the course of an invasive C. albicans infection and elicits a specific antibody response. In this study, we u...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.469-477.1981
更新日期:1981-11-01 00:00:00
abstract::In vitro experimentation indicates that periodontitis-associated bacteria contain potent polyclonal B-cell activators (PBA). We reasoned that if PBA were operative in vivo, plasma cells specific for nonoral antigens should be present in the inflamed gingival tissues, which are characterized by a plasma cell infiltrate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.823-830.1988
更新日期:1988-04-01 00:00:00
abstract::No studies to date clearly define the interactions between Porphyromonas gingivalis and human peripheral blood polymorphonuclear leukocytes (PMN), nor has a protective role for antibody to P. gingivalis been defined. Using a fluorochrome phagocytosis microassay, we investigated PMN phagocytosis and killing of P. gingi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.2097-2104.1991
更新日期:1991-06-01 00:00:00
abstract::The development of immunological responsiveness to a soluble cercarial antigenic preparation (CAP) from Schistosoma mansoni was analyzed in inbred CBA/J mice infected with cercariae, one or multiple times, or sensitized using CAP. Repeated exposure to 75 cercariae at three weekly intervals (3X-75) or subcutaneous admi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.2.502-508.1976
更新日期:1976-08-01 00:00:00
abstract::The within-host dynamics of an infection with the malaria parasite Plasmodium falciparum are the result of a complex interplay between the host immune system and parasite. Continual variation of the P. falciparum erythrocyte membrane protein (PfEMP1) antigens displayed on the surface of infected red blood cells enable...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00958-13
更新日期:2014-01-01 00:00:00
abstract::Three types of caseinolytic proteases (Pase-A, Pase-B, and Pase-C) were isolated and purified from culture supernatants of Porphyromonas gingivalis 381 by the combined procedures of acetone precipitation, gel filtration, solubilization with octylthioglucoside followed by affinity chromatography on arginine-Sepharose 4...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.3060-3068.1991
更新日期:1991-09-01 00:00:00
abstract::A spot enzyme-linked immunosorbent assay was used to show that subcutaneous inoculation of a sublethal number of Listeria monocytogenes resulted in the early appearance of gamma interferon (IFN-gamma)-producing cells in the draining lymph nodes. In contrast, inoculation of UV-killed L. monocytogenes failed to cause th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.2892-2900.1991
更新日期:1991-09-01 00:00:00
abstract::A total of 32 patients with Plasmodium falciparum malaria were studied. Of these, 23 had benign infections, and 9 had typical cerebral malaria. Cryoglobulins, circulating immune complexes detected by a C1q-binding assay, and hypocomplementemia were found in eight of nine patients with cerebral malaria. Raised levels o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.530-535.1981
更新日期:1981-02-01 00:00:00
abstract::Acquired cellular immunity to infection with Mycobacterium tuberculosis is believed to reside in the capacity of mononuclear phagocytes of immunized animals to inhibit intracellular multiplication of the parasite. However, in macrophage tissue culture systems, it has been customary to employ streptomycin in the medium...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1970-01-01 00:00:00
abstract::Mice immunized subcutaneously with heat-killed Escherichia coli were protected from pyelonephritis produced by the intravenous route, but there was little or no protection from ascending infection. No significant protection from ascending or hematogenous pyelonephritis was demonstrated when immunization was accomplish...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.3.330-334.1972
更新日期:1972-09-01 00:00:00
abstract::A genomic library of meningococcal DNA from a clinical isolate of Neisseria meningitidis was constructed in the expression vector lambda gt11. Outer membrane complex was prepared from the same strain and used to immunize rabbits to raise polyclonal anti-outer membrane complex serum. The amplified library was probed wi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.11.2734-2740.1987
更新日期:1987-11-01 00:00:00
abstract::Osteoarticular brucellosis is the most common complication in Brucella-infected humans regardless of age, sex, or immune status. The mechanism of bone destruction caused by Brucella species remained partially unknown due to the lack of a suitable animal model. Here, to study this complication, we explored the suitabil...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00901-18
更新日期:2019-05-21 00:00:00
abstract::Studies of active immunization against Helicobacter pylori indicate that antibodies play a minor role in immunity. There is also evidence, however, that the translocation of antibodies in the stomach may be insufficient to achieve functional antibody levels in the gastric lumen. We have used a suckling mouse model of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00547-09
更新日期:2009-11-01 00:00:00
abstract::B-cell-deficient mice were prepared by administration of rabbit anti-mouse-mu antiserum to newborn animals within 12 h of birth onwards. Such immunodeficient animals, along with the normal controls, were infected intravenously with Cryptococcus neoformans. There was no difference in the mortality pattern, viable count...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.1-3.1979
更新日期:1979-10-01 00:00:00
abstract::Mouse spleen cells which normally cannot support the in vivo replication of vesicular stomatitis virus (VSV) became susceptible to VSV infection after the intraperitoneal growth of certain syngeneic and allogeneic tumors. After 3 days' growth of P815 tumor cells in syngeneic DBA/2 mice, the viral-permissive state for ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.3.1014-1023.1981
更新日期:1981-06-01 00:00:00
abstract::Resistance to cutaneous syphilitic reinfection in strain 2 and strain 13 guinea pigs developed gradually 3 to 7 months after primary infection and reached maximum levels at 6 to 7 months after the induction of primary cutaneous disease. Associated with this acquired resistance was the occurrence of Arthus reactions an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.66-72.1985
更新日期:1985-10-01 00:00:00
abstract::Previously we demonstrated that recombinant murine interleukin-12 (rmIL-12) administration can promote a primary Th1 response while suppressing the Th2 response in mice primed with 2,4, 6-trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH). The present studies examined the capacity of rmIL-12 to drive a Th1 response to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.5.2166-2171.1999
更新日期:1999-05-01 00:00:00
abstract::No effective therapy exists for Cryptosporidium parvum, a coccidial protozoan parasite that causes severe diarrhea in patients with AIDS. The role of microtubules in parasite invasion of host cells was investigated by incubating 10(7) oocysts with a HT 29.74 cell line for 24 h in the presence of microtubule-disrupting...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4888-4890.1993
更新日期:1993-11-01 00:00:00
abstract::This study investigates the role of CD4+ T cells in host defense against cholera enterotoxin-induced diarrhea. Antitoxin immunoglobulin A formation and gut protection against cholera toxin (CT) following oral immunizations with CT were evaluated in normal mice and mice that had been depleted of CD4+ T cells by in vivo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3630-3638.1991
更新日期:1991-10-01 00:00:00
abstract::Mice with disrupted beta 2-microglobulin (beta 2m-/-), I-A (class II-/-), or CD4 (CD4-/-) genes were examined for their capacity to resolve Chlamydia trachomatis genital tract infection. C57BL/6 and beta 2m-/- mice resolved infection similarly and were culture negative by 4 to 5 weeks following infection. Conversely, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4661-4668.1995
更新日期:1995-12-01 00:00:00
abstract::Streptococcus suis serotype 2 is a porcine and human pathogen with adhesive and invasive properties. In other streptococci, large surface-associated proteins (>100 kDa) of the MSCRAMM family (microbial surface components recognizing adhesive matrix molecules) are key players in interactions with host tissue. In this s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00359-06
更新日期:2006-11-01 00:00:00
abstract::We used the Salmonella typhi galactose epimerase (galE) mutant strain Ty21a, shown to be a safe, effective, living, attenuated oral typhoid vaccine, as a recipient for a recombinant plasmid containing the gene for production of the nontoxic B subunit of the heat-labile enterotoxin of Escherichia coli. The S. typhi der...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.564-569.1984
更新日期:1984-11-01 00:00:00
abstract::Streptolysin O (SLO) and streptolysin S (SLS) are potent cytolytic toxins produced by almost all clinical isolates of group A streptococci (GAS). Allele-replacement mutagenesis was used to construct nonpolar (in-frame) deletion mutations in the slo and sagB genes of the serotype M5 GAS strain Manfredo, producing isoge...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.3857-3865.2003
更新日期:2003-07-01 00:00:00