Abstract:
:Raising the magnesium level from 0.4 to 1.5 mM in a medium containing only amino acids (2.08%), salts, and vitamins increased enterotoxin B production by Staphylococcus aureus S-6 by about 80%. The level of phosphates in the medium was lowered to one-tenth (to 2.87 mM) the original amount without adversely affecting growth and enterotoxin production. The optimum level of potassium was between 15 and 45 mM. Ammonium salts were not essential in the amino acid medium. Sufficient quantities of trace metals were already present. Revising the salt mixture according to the information obtained resulted in a doubling of the enterotoxin B production.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Keller GM,Hanson RS,Bergdoll MSdoi
10.1128/IAI.20.1.158-160.1978subject
Has Abstractpub_date
1978-04-01 00:00:00pages
158-60issue
1eissn
0019-9567issn
1098-5522journal_volume
20pub_type
杂志文章abstract::Reduced-minus-oxidized difference spectra of sonically treated virulent Treponema pallidum disclosed cytochromes of the b anc c types as well as large amounts of flavoprotein. Difference spectra of the carbon monoxide-binding pigment identified cytochrome o as the terminal oxidase. Physiological reduction of the cytoc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.3.885-890.1977
更新日期:1977-06-01 00:00:00
abstract::Serum antibody responses to toxic shock syndrome (TSS) toxin 1 (TSST-1) and staphylococcal enterotoxins A, B, and C were determined by western blot (immunoblot) analysis of acute- and convalescent-phase paired sera from 18 TSS- and 31 non-TSS-associated Staphylococcus aureus infections. Compared with non-TSS cases, se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.1.231-234.1989
更新日期:1989-01-01 00:00:00
abstract::A peptidoglycan layer of Treponema pallidum kazan was isolated by solubilization of whole cells with 1% warm sodium dodecyl sulfate and subsequent digestion of an insoluble residue with proteases. Electron microscopy revealed that the peptidoglycan was isolated as a single-layered sacculus of less than 5 nm in thickne...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.767-774.1981
更新日期:1981-02-01 00:00:00
abstract::γ9δ2 T cells provide a natural bridge between innate and adaptive immunity, rapidly and potently respond to pathogen infection in mucosal tissues, and are prominently induced by both tuberculosis (TB) infection and bacillus Calmette Guérin (BCG) vaccination. Mycobacterium-expanded γ9δ2 T cells represent only a subset ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01322-15
更新日期:2016-08-19 00:00:00
abstract::One of the first steps in the development of cerebral toxoplasmosis is the penetration of the blood-brain barrier, which is comprised of microvascular endothelial cells. We examined the capacity of human brain microvascular endothelial cells (HBMEC) to interact with Toxoplasma gondii. We found that stimulation of HBME...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.10.6527-6531.2001
更新日期:2001-10-01 00:00:00
abstract::The Salmonella dublin virulence plasmid mediates systemic infection in mice and cattle. Here, we analyze the interaction between wild-type and plasmid-cured Salmonella strains with phagocytes in vitro and in vivo. The intracellular recovery of S. dublin from murine peritoneal and bovine alveolar macrophages cultured i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3385-3393.1996
更新日期:1996-08-01 00:00:00
abstract::The fucose-mannose ligand (FML) complex of Leishmania donovani is a promising vaccine candidate against murine and canine visceral leishmaniasis, and its main component is a 36-kDa nucleoside hydrolase (NH36). In this study, we tested the immune response and protection induced by the purified FML, the recombinant NH36...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.2.812-819.2005
更新日期:2005-02-01 00:00:00
abstract::Human and rat C-reactive proteins, major acute-phase reactants, bind to sporozoites and inhibit their in vitro development in hepatocytes (A. Nussler, S. Pied, M. Pontet, F. Miltgen, L. Renia, M. Gentilini, and D. Mazier, Exp. Parasitol. 72:1-7, 1991, and S. Pied, A. Nussler, M. Pontet, F. Miltgen, H. Matile, P.-H. La...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.348-349.1993
更新日期:1993-01-01 00:00:00
abstract::Adult volunteers received a booster dose (4 x 10(8) CFU) of attenuated Vibrio cholerae CVD 103-HgR oral vaccine 15 or 24 months after primary immunization. The immune response was modest, presumably due to rapid clearance of the vaccine strain by a primed immune system. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.9.3916-3917.1992
更新日期:1992-09-01 00:00:00
abstract::Recent reports have demonstrated that Bordetella pertussis has invasive behavior in vivo and in vitro. In this study, we investigated the ability of a virulent strain, avirulent mutants, and mutants deficient in specific virulence factors to enter and survive intracellularly in human macrophages in vitro. Uptake of vi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4578-4585.1992
更新日期:1992-11-01 00:00:00
abstract::Historically, Pneumocystis carinii pneumonia has been the most frequent cause of morbidity and mortality in patients with AIDS. Antiprotozoan drugs are effective in the treatment and prophylaxis of P. carinii pneumonia, which lends credence to the widely held view that P. carinii is a protozoan. However, recent geneti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.10.4140-4145.1992
更新日期:1992-10-01 00:00:00
abstract::Sepsis is both a common and, despite present-day therapy, a serious disease. The pathophysiology of the septic response is a complex, multifactorial phenomenon which in part involves the activation of complement by bacterial endotoxin. A monoclonal antibody to human complement factor B, code-named 1H5, which was capab...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4549-4555.1994
更新日期:1994-10-01 00:00:00
abstract::Cultures of Serpulina pilosicoli 95/1000, isolated from a pig with porcine intestinal spirochetosis (PIS), and S. pilosicoli WesB, isolated from an Aboriginal child with diarrhea, were used to infect 5-week-old newly weaned pigs. Four of 12 pigs infected with strain 95/1000 and 2 of 12 pigs infected with strain WesB b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4648-4654.1996
更新日期:1996-11-01 00:00:00
abstract::Adherence of blastoconidia to epidermal corneocytes is an early event in Candida colonization and infection of the skin. Pathogenic species adhere more avidly than nonpathogenic species, transform to hyphal growth, and invade the stratum corneum of the skin. Adherence was studied by scanning electron microscopy of exp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.1942-1949.1988
更新日期:1988-08-01 00:00:00
abstract::Cholera is an acute diarrheal disease that is caused by the gram-negative bacterium Vibrio cholerae. The low efficacy of currently available killed-whole-cell vaccines and the reactinogenicity coupled with potential reversion of live vaccines have thus far precluded widespread vaccination for the control of cholera. R...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7695-7702.2001
更新日期:2001-12-01 00:00:00
abstract::This report describes an attempt to define the factors which incite secondary bacterial pneumonias. Groups of mice were given bacteria intraperitoneally and, at various intervals, Newcastle disease virus intravenously. There was an increase in the number of deaths and in the rates of death in these groups, when compar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.3.488-493.1971
更新日期:1971-03-01 00:00:00
abstract::Pyolysin (PLO), a cholesterol-dependent cytolysin expressed by Arcanobacterium pyogenes, is an important host-protective antigen. However, this molecule is toxic and requires inactivation prior to its use as a vaccine. Three genetically toxoided, nonhemolytic PLO molecules, HIS-PLO.F(497), HIS-PLO.Delta P(499), and HI...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2966-2969.2003
更新日期:2003-05-01 00:00:00
abstract::Tissues and organs provide the structural and biochemical landscapes upon which microbial pathogens and commensals function to regulate health and disease. While flat two-dimensional (2-D) monolayers composed of a single cell type have provided important insight into understanding host-pathogen interactions and infect...
journal_title:Infection and immunity
pub_type: 历史文章,杂志文章,评审
doi:10.1128/IAI.00282-18
更新日期:2018-10-25 00:00:00
abstract::The amino acid sequence of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli 18D has been revised. Amino acids originally assigned to positions 11 and 18, i.e., Tyr and Asn, respectively, were found to occupy positions 18 and 11, respectively. Thus all heat-stable enterotoxins composed of 18 amino a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.3.834-836.1985
更新日期:1985-03-01 00:00:00
abstract::The combining sites of type 1 fimbrial lectins of various species of enterobacteria were studied by measuring the inhibitory activity of linear and branched oligosaccharides and several glycosides of D-mannose on the agglutination of yeast cells by the organisms. The results showed that all five strains of Escherichia...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1088-1090.1984
更新日期:1984-03-01 00:00:00
abstract::Clostridium perfringens enterotoxin is the major virulence factor involved in the pathogenesis of C. perfringens type A food poisoning and several non-food-borne human gastrointestinal illnesses. The enterotoxin gene, cpe, is located on the chromosome of food-poisoning isolates but is found on a large plasmid in non-f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3483-3487.2001
更新日期:2001-05-01 00:00:00
abstract::The Toll-like receptor 2 (TLR2)/TLR1 receptor complex responds to amyloid fibrils, a common component of biofilm material produced by members of the phyla Firmicutes, Bacteroidetes, and Proteobacteria. To determine whether this TLR2/TLR1 ligand stimulates inflammatory responses when bacteria enter intestinal tissue, w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00911-12
更新日期:2012-12-01 00:00:00
abstract::The gene encoding Clostridium sordellii phospholipase C (Csp) was cloned and expressed as a histidine-tagged (His-tag) protein, and the protein was purified to compare its enzymatic and biological activities with those of Clostridium perfringens phospholipase C (Cpa) and Clostridium bifermentans phospholipase C (Cbp)....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.2.641-646.2003
更新日期:2003-02-01 00:00:00
abstract::Human peripheral blood monocytes become apoptotic following phagocytosis of Staphylococcus aureus. In this study, we investigated the mechanisms involved in this phenomenon. Cells exposed to bacteria were examined for the surface expression of Fas and Fas ligand (FasL). The level of soluble form of FasL was also measu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1287-1297.2001
更新日期:2001-03-01 00:00:00
abstract::A synthetic medium capable of supporting growth of Vibrio parahaemolyticus is described. Growth yields and generation times were comparable to growth in a complex medium, although Kanagawa hemolysin was undetectable in the synthetic medium. Upon the addition of single amino acids to this synthetic medium, only D-trypt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.60-63.1982
更新日期:1982-07-01 00:00:00
abstract::Early-onset periodontitis (EOP) is characterized by rapidly progressive alveolar bone loss, chemotactic defects of neutrophils, and significant familial aggregation. We found immature myeloid lineage cells, defined as promyelocytes, in the peripheral blood in patients with EOP. A hematological examination of periphera...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3906-3912.1997
更新日期:1997-09-01 00:00:00
abstract::Cell-mediated immunity is critical in host resistance against the pathogenic fungus Histoplasma capsulatum. To explore the role of L3T4+ T cells in protection of mice against H. capsulatum infection, we examined the effect of in vivo treatment with anti-L3T4 monoclonal antibody (MAb) GK1.5 on the course of murine diss...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1685-1691.1988
更新日期:1988-07-01 00:00:00
abstract::A virus, 78-238, isolated from the cerebrospinal fluid of a dog with neurological dysfunction, was characterized as a paramyxovirus. This conclusion was supported by viral cytopathic effects and morphological appearance of virions and nucleocapsids in infected cells. Nucleocapsids were found in the cytoplasm of all in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.1177-1183.1981
更新日期:1981-03-01 00:00:00
abstract::Acinetobacter baumannii is a pathogen of increasing medical importance with a propensity to be multidrug resistant, thereby making treatment challenging. Little is known of virulence traits in A. baumannii. To identify virulence factors and potential drug targets, random transposon (Tn) mutants derived from the A. bau...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00366-10
更新日期:2010-09-01 00:00:00
abstract::The extracellular adherence protein (Eap) is a multifunctional Staphylococcus aureus protein and broad-spectrum adhesin for several host matrix and plasma proteins. We investigated the interactions of full-length Eap and five recombinant tandem repeat domains with host proteins by use of surface plasmon resonance (BIA...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00480-08
更新日期:2008-12-01 00:00:00