Abstract:
:Twenty monoclonal antibodies have been prepared to the erythrocytes from CBA/Ca mice infected with the rodent malaria Plasmodium chabaudi. By immunofluorescence, 15 of these antibodies recognized parasite antigens expressed only during the development of mature trophozoites to schizonts and merozoites, 2 recognized parasite antigens that were expressed throughout most of the intraerythrocytic cycle, and 3 recognized the membranes of all infected and uninfected erythrocytes. By immunoprecipitation of [35S]methionine-labeled, parasitized erythrocytes, parasite antigens recognized by all of the antiparasite antibodies were characterized. Eleven precipitated a 250,000-dalton parasite polypeptide which was synthesized and expressed late in the intraerythrocytic cell cycle and which appeared to be the major coat protein of the merozoites. In passive protection experiments, transfer of hyperimmune serum before infection with the parasite resulted in a delay in the rise of parasitemia, reduction in peak parasitemias, and a delay in the clearance of the parasitemia. Two monoclonal antibodies to the 250,000-dalton polypeptide had a similar but not as marked effect on parasitemia when given as a single dose before infection. When mixed and administered throughout the course of infection, their effects were greater. They had no influence on the course of Plasmodium berghei KSP11 parasitemia. Monoclonal antibodies to other parasite antigens and normal erythrocyte antigens failed to have a significant and reproducible effect on P. chabaudi parasitemia. The results suggest that this 250,000-dalton malaria parasite antigen may be important in the induction and expression of antibody-mediated immunity to malaria.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Boyle DB,Newbold CI,Smith CC,Brown KNdoi
10.1128/IAI.38.1.94-102.1982subject
Has Abstractpub_date
1982-10-01 00:00:00pages
94-102issue
1eissn
0019-9567issn
1098-5522journal_volume
38pub_type
杂志文章abstract::The C-terminal 19-kDa domain of merozoite surface protein 1 (MSP1₁₉) is the target of protective antibodies but alone is poorly immunogenic. Previously, using the Plasmodium yoelii murine model, we fused P. yoelii MSP1₁₉ (PyMSP1₁₉) with full-length P. yoelii merozoite surface protein 8 (MSP8). Upon immunization, the M...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00211-12
更新日期:2012-07-01 00:00:00
abstract::Gastroesophageal reflux (GER) frequently occurs in patients with respiratory disease and is particularly prevalent in patients with cystic fibrosis. GER is a condition in which the duodenogastric contents of the stomach leak into the esophagus, in many cases resulting in aspiration into the respiratory tract. As such,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00674-13
更新日期:2014-09-01 00:00:00
abstract::The humoral and cell-mediated immune responses of murine lymphocytes exposed to lead in vivo and in vitro were investigated. In vivo Pb was administered via the drinking water (0 to 10 mM) for 1 to 10 weeks. In vivo exposure of the mice to Pb did not alter significantly their plaque-forming cell response to sheep eryt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.136-143.1981
更新日期:1981-01-01 00:00:00
abstract::An exoprotein of Staphylococcus aureus 100 that elicited a positive ileal loop response in the rabbit model was investigated in this study. The protein, as it occurred in the culture supernatant fluid, could be detected initially in the late log phase of growth under aerobic and anaerobic conditions. It was stable und...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.2.298-303.1977
更新日期:1977-11-01 00:00:00
abstract::Porphyromonas gingivalis, a gram-negative anaerobic bacterium associated with active lesions of chronic periodontitis, produces several proteinases which are presumably involved in host colonization, perturbation of the immune system, and tissue destruction. The aims of this study were to investigate the degradation o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4351-4356.2004
更新日期:2004-08-01 00:00:00
abstract::Chronic lung infections with Pseudomonas aeruginosa biofilms are associated with refractory and fatal pneumonia in cystic fibrosis (CF). In this study, a group of genomically diverse P. aeruginosa isolates were compared with the reference strain PAO1 to assess the roles of motility, twitching, growth rate, and overpro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.1.133-144.2004
更新日期:2004-01-01 00:00:00
abstract::Conjunctival infection of guinea pigs by the chlamydial agent of guinea pig inclusion conjunctivitis confers immunity. However, the mechanism of resistance to this intracellular pathogen is not yet defined. In the study reported here, serum immunoglobulin was passively transferred with resultant titers in excess of th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.4.597-599.1973
更新日期:1973-04-01 00:00:00
abstract::The influences of Neisseria gonorrhoeae protein IB content and bilayer composition of liposomes and protein content of iscoms on immunogenicity were investigated. Changes in the protein content of liposomes did not influence the immunoglobulin G response, whereas the response was lowered when the amount of protein in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.6.1661-1664.1988
更新日期:1988-06-01 00:00:00
abstract::The results show that Listeria monocytogenes, Francisella tularensis, and Salmonella typhimurium are facultative intracellular bacteria with a capacity to invade and grow in nonphagocytic cells in vivo. In the liver, all of these pathogens were seen to invade and to multiply extensively in hepatocytes. In all three ca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5164-5171.1992
更新日期:1992-12-01 00:00:00
abstract::Pathogenic Leptospira spp. are likely to encounter higher concentrations of reactive oxygen species induced by the host innate immune response. In this study, we characterized Leptospira interrogans catalase (KatE), the only annotated catalase found within pathogenic Leptospira species, by assessing its role in resist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00466-12
更新日期:2012-11-01 00:00:00
abstract::Salmonella enterica serovar Paratyphi A O-specific polysaccharide (O-SP) was activated with 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) and bound to tetanus toxoid (TT) with adipic acid dihydrazide as a linker (SPA-TT(1)) or directly (SPA-TT(2)). In mice, these two conjugates elicited high levels of imm...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/iai.68.3.1529-1534.2000
更新日期:2000-03-01 00:00:00
abstract::Thirty-two clinical isolates of Shiga-like toxin (SLT)-producing Escherichia coli associated with single cases or outbreaks of bloody diarrhea, hemorrhagic colitis, the hemolytic uremic syndrome, or edema disease of swine were examined for multiple copies of genes belonging to the slt-I or slt-II toxin families. Five ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.1065-1073.1991
更新日期:1991-03-01 00:00:00
abstract::To study the immunological activity of proteins secreted by Mycobacterium tuberculosis, we carried out comparative studies in guinea pigs infected intravenously with 2.5 x 10(3) CFU of this organism or with 2.5 x 10(4) CFU of Mycobacterium bovis BCG. Groups of infected guinea pigs were skin tested with fractions of se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.804-810.1995
更新日期:1995-03-01 00:00:00
abstract::Biofilm formation by the periodontal pathogen Aggregatibacter actinomycetemcomitans is dependent upon autoinducer-2 (AI-2)-mediated quorum sensing. However, the components that link the detection of the AI-2 signal to downstream gene expression have not been determined. One potential regulator is the QseBC two-compone...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01376-09
更新日期:2010-07-01 00:00:00
abstract::Pro- and anti-inflammatory cytokines and their signaling pathways play key roles in protection from and pathogenesis of mycobacterial infection, and their balance and dynamic changes may control or predict clinical outcome. Peripheral blood cells' capacity to produce proinflammatory (tumor necrosis factor alpha [TNF-a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00602-06
更新日期:2007-02-01 00:00:00
abstract::To evaluate the enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides, the rate of isolation of these organisms was compared among individuals with and without diarrhea in Thailand. In two groups of American travelers, A. hydrophila, but not P. shigelloides, was associated with episodes of travelers...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.666-673.1982
更新日期:1982-02-01 00:00:00
abstract::The P2 porin protein is the major outer membrane protein of nontypeable Haemophilus influenzae and is a potential target of a protective immune response. Nine monoclonal antibodies (MAbs) to P2 were developed by immunizing mice with nontypeable H. influenzae whole organisms. Each MAb reacted exclusively with the homol...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3712-3722.1994
更新日期:1994-09-01 00:00:00
abstract::Comparisons of the course of coccidioidomycosis in two strains of inbred mice established that BALB/c mice are significantly more susceptible to pulmonary infection with Coccidioides immitis than are DBA/2 mice. The susceptibility of BALB/c mice does not reside in their inability to mount a delayed-type hypersensitivi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.1.13-17.1988
更新日期:1988-01-01 00:00:00
abstract::Bacteria alter their cell surface in response to changing environments, including those encountered upon invasion of a host during infection. One alteration that occurs in several Gram-positive pathogens is the presentation of cell wall-anchored components of the iron-regulated surface determinant (Isd) system, which ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00397-15
更新日期:2015-09-01 00:00:00
abstract::After interaction with tracheal epithelial cells, Bordetella pertussis induces the secretion of interleukin-6. This secretion is dependent on the expression of adenylate cyclase-hemolysin by the bacterium but not on the expression of other characterized bacterial toxins or adhesins. This finding confirms the important...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5530-5533.2004
更新日期:2004-09-01 00:00:00
abstract::Lactoperoxidase catalyzes the oxidation of thiocyanate by hydrogen peroxide into hypothiocyanite, a reaction which can protect bacterial and mammalian cells from killing by hydrogen peroxide. The present study demonstrates, however, that lactoperoxidase in the presence of thiocyanate can actually potentiate the bacter...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.3.581-586.1984
更新日期:1984-06-01 00:00:00
abstract::To test the hypothesis that the Staphylococcus aureus enterotoxin gene cluster (egc) can generate new enterotoxin genes by recombination, we analyzed the egc locus in a broad panel of 666 clinical isolates of S. aureus. egc was present in 63% of isolates, confirming its high prevalence. The archetypal organization of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00132-06
更新日期:2006-08-01 00:00:00
abstract::Nonspecific interactions related to physicochemical properties of bacterial cell surfaces, such as hydrophobicity and electrostatic charge, are known to have important roles in bacterium-host cell encounters. Streptococcus pneumoniae (pneumococcus) expresses multiple, surface-exposed, choline-binding proteins (CBPs) w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.1.412-415.2002
更新日期:2002-01-01 00:00:00
abstract::Actinobacillus pleuropneumoniae can use porcine transferrin as the sole source of iron. Two proteins with molecular masses of approximately 60 kDa (TfbA) and 110 kDa have been shown to specifically bind porcine transferrin; from the TfbA protein, three isoforms from A. pleuropneumoniae serotypes 1, 5, and 7 have been ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.3846-3850.1995
更新日期:1995-10-01 00:00:00
abstract::When inoculated orally, Escherichia coli strain 1056 caused acute enteritis in 7 of 22 weanling hamsters. E. coli strain 1056 was isolated from the ileum of a hamster with proliferative ileitis. It was lactose negative, nonmotile, and anaerogenic. By electron microscopy and indirect fluorescent-antibody techniques, E....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.1232-1238.1981
更新日期:1981-03-01 00:00:00
abstract::Bacterial tyrosine kinases and their cognate protein tyrosine phosphatases are best known for regulating the biosynthesis of polysaccharides. Moreover, their roles in the stress response, DNA metabolism, cell division, and virulence have also been documented. The aim of this study was to investigate the pathogenicity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02713-14
更新日期:2015-02-01 00:00:00
abstract::Patients with chronic bronchitis are colonized by and may develop acute bronchopulmonary infection due to nontypable Haemophilus influenzae (NTHI) despite the presence of bactericidal and opsonizing antibody to the infecting organism. To test the hypothesis that secretory immunoglobulin A (IgA) interferes with host de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.36-40.1984
更新日期:1984-07-01 00:00:00
abstract::Spiroplasmas were propagated in the Drosophila melanogaster cell line Dm-1. Spiroplasma citri and unidentified strains (corn shunt organism, 277F [tick isolate], powder puff, BNR-1, honey bee, and OBMG) grew to 10(8) to 10(9) colony-forming units per ml and could be passaged. Cytopathic effect (CPE) varied with the in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.296-304.1982
更新日期:1982-01-01 00:00:00
abstract::Rubella virus was capable of replicating in both unstimulated and phytohemagglutinin-stimulated cultures of human mononuclear blood cells. Monocyte-derived macrophages were the main cell type responsible for viral replication. The susceptibility of macrophages increased during cultivation. Phytohemagglutinin-stimulate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.309-314.1980
更新日期:1980-02-01 00:00:00
abstract::Peptic ulcer disease (PUD) occurs after a long-term Helicobacter pylori infection. However, the disease can develop earlier, and rare cases have been observed in children, suggesting that these H. pylori strains may be more virulent. We used suppressive subtractive hybridization for comparative genomics between H. pyl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00123-06
更新日期:2006-07-01 00:00:00