Abstract:
:Nonspecific interactions related to physicochemical properties of bacterial cell surfaces, such as hydrophobicity and electrostatic charge, are known to have important roles in bacterium-host cell encounters. Streptococcus pneumoniae (pneumococcus) expresses multiple, surface-exposed, choline-binding proteins (CBPs) which have been associated with adhesion and virulence. The purpose of this study was to determine the contribution of CBPs to the surface characteristics of pneumococci and, consequently, to learn how CBPs may affect nonspecific interactions with host cells. Pneumococcal strains lacking CBPs were derived by adapting bacteria to a defined medium that substituted ethanolamine for choline. Such strains do not anchor CBPs to their surface. Cell surface hydrophobicity was tested for the wild-type and adapted strains by using a biphasic hydrocarbon adherence assay, and electrostatic charge was determined by zeta potential measurement. Adherence of pneumococci to human-derived cells was assessed by fluorescence-activated cell sorter analysis. Strains lacking both capsule and CBPs were significantly more hydrophobic than nonencapsulated strains with a normal complement of CBPs. The effect of CBPs on hydrophobicity was attenuated in the presence of capsule. Removal of CBPs conferred a greater electronegative net surface charge than that which cells with CBPs possessed, regardless of the presence of capsule. Strains that lack CBPs were poorly adherent to human monocyte-like cells when compared with wild-type bacteria with a full complement of CBPs. These results suggest that CBPs contribute significantly to the hydrophobic and electrostatic surface characteristics of pneumococci and may facilitate adherence to host cells partially through nonspecific, physicochemical interactions.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Swiatlo E,Champlin FR,Holman SC,Wilson WW,Watt JMdoi
10.1128/iai.70.1.412-415.2002keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
412-5issue
1eissn
0019-9567issn
1098-5522journal_volume
70pub_type
杂志文章abstract::Brewer thioglycolate medium, a bacterial culture medium which is widely used as a nonspecific in vivo macrophage stimulant, was tested for its effect on mouse resistance to bacterial infection. Mice which did or did not receive thioglycolate medium were challenged with Listeria monocytogenes. Injection of thioglycolat...
journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1982-02-01 00:00:00
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更新日期:1998-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1990-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1990-08-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1973-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1995-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.6.937-940.1973
更新日期:1973-06-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1979-01-01 00:00:00
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更新日期:2005-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2018-03-22 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1989-06-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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pub_type: 杂志文章
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journal_title:Infection and immunity
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更新日期:1987-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1991-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
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更新日期:2006-03-01 00:00:00
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更新日期:2006-01-01 00:00:00