Abstract:
:A protein factor which induces high levels of gamma interferon (IFN-gamma) in resting splenic nonadherent cells was isolated from the sera of mice with generalized inflammation caused by endotoxic shock. The factor was highly purified by ammonium sulfate precipitation followed by ion-exchange column chromatography on DEAE-Sepharose, molecular sieving on Ultrogel AcA 44, and hydrophobic column chromatography with phenyl-Sepharose. It was further purified to apparent homogeneity by polyacrylamide gel electrophoresis. It induced IFN-gamma production in a dose-dependent manner in the presence of interleukin-2, monoclonal anti-CD3 antibody (anti-CD3 MAb), or concanavalin A (ConA) in spleen cells deprived of plastic plate- and nylon wool-adherent cells. Anti-CD3 MAb induced the highest level of production of the three. The factor, interleukin-2, anti-CD3 MAb, or ConA alone induced a trace of or no detectable IFN-gamma in these cells. The factor also exhibited an accessory function during proliferation in these cells in the presence of a suboptimal dose of ConA. However, the factor failed to stimulate IFN-gamma production when staphylococcal enterotoxin A, a superantigenic T-cell mitogen, was employed. Treatment with pronase or heat abolished these activities. These studies confirm the existence of a soluble protein factor which is able to exhibit a novel accessory function in IFN-gamma production in resting T or natural killer cells. It will be of interest to compare this factor with the recently cloned human natural killer stimulatory factor (NKSF/IL-12).
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Nakamura K,Okamura H,Nagata K,Komatsu T,Tamura Tdoi
10.1128/IAI.61.1.64-70.1993subject
Has Abstractpub_date
1993-01-01 00:00:00pages
64-70issue
1eissn
0019-9567issn
1098-5522journal_volume
61pub_type
杂志文章abstract::The quantity of membrane-bound and extracellular exotoxin A in four strains of Pseudomonas aeruginosa was investigated. In strain PA-103, which is the prototype strain used for toxin production, all of the toxin was released into the growth medium and little toxin remained with the cell envelope. In a virulent strain ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::The possibility that soluble products of Schistosoma mansoni eggs might participate in the pathogenesis of hepatic fibrosis in schistosomiasis was investigated. Both crude saline extracts of eggs (soluble egg antigen [SEA]) and a partially purified SEA fraction contained activity which stimulated guinea pig and human ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.103-108.1982
更新日期:1982-10-01 00:00:00
abstract::Genomic DNA fingerprint analysis was performed on 39 Staphylococcus aureus and 28 Enterococcus faecalis endophthalmitis isolates collected from multiple clinical centers. Among 21 S. aureus genomic DNA fingerprint patterns identified, five clonotypes were recovered from multiple unrelated patients and accounted for 58...
journal_title:Infection and immunity
pub_type: 杂志文章,多中心研究
doi:10.1128/IAI.66.1.356-360.1998
更新日期:1998-01-01 00:00:00
abstract::We demonstrate that 2-month-old female B10.T(6R) mice are highly resistant to systemic infection with the KIM5 strain of Yersinia pestis and that B10.T(6R) mice become susceptible to Y. pestis infection by the age of 5 months. In this study, young (2-month-old) and middle-aged (5- to 12-month-old) B10.T(6R) mice were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05267-11
更新日期:2011-11-01 00:00:00
abstract::Neisseria meningitidis is a human-specific pathogen and leading cause of meningitis and septicemia. Factor H binding protein (fHbp), a virulence factor which protects N. meningitidis from innate immunity by binding the human complement regulator factor H (fH) with high affinity, is also a key antigen in vaccines being...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01183-13
更新日期:2014-03-01 00:00:00
abstract::Vibrio cholerae O1 bacteria harvested directly from ligated or nonligated intestines of rabbits with experimental cholera expressed at least 7 to 8 novel, in vivo-specific cell envelope (env) proteins that were not found on vibrios after in vitro culture in various ordinary liquid media. At the same time, several of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1809-1815.1989
更新日期:1989-06-01 00:00:00
abstract::Chromatographic analysis, coupled to mass spectrometry with a high-resolution mass spectrometer, of materials isolated from skin lesions of patients with lepromatous leprosy allows the recognition of characteristic mycobacterial products, mycolic acids. This finding indicates that the "noncultivable" bacteria responsi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.236-239.1974
更新日期:1974-07-01 00:00:00
abstract::Monoclonal antibodies to Trichomonas vaginalis were prepared by immunizing mice with a cloned isolate of T. vaginalis. Eight antibodies reacted with the same four isolates or strains but did not react with the other T. vaginalis strains or isolates tested. All eight antibodies reacted uniformly with both the body and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.270-275.1984
更新日期:1984-01-01 00:00:00
abstract::V beta 10(+) and V beta 14(+) T cells were selectively increased 7 to 14 days following infection in the lungs of naive mice infected with Histoplasma capsulatum. Following secondary challenge of immune mice, V beta 1(+) and V beta 8.1(+) cells were sporadically increased. Elimination of V beta 10(+) and V beta 14(+) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1648-1652.2002
更新日期:2002-03-01 00:00:00
abstract::We found that oral immunization with flagellum-defective mutant strains of Salmonella enterica serovar Typhimurium with the ClpXP-deficient background protected mice against oral challenge with the virulent strain. These data indicate that Salmonella flagellin is not a dominant protective antigen in oral immunization ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2449-2451.2004
更新日期:2004-04-01 00:00:00
abstract::We previously demonstrated the suppression of cell-mediated immunity to Listeria monocytogenes by Pseudomonas aeruginosa-induced, macrophage-like cells. The present study was undertaken to evaluate the mechanism for this suppression. P. aeruginosa supernatant was shown to activate macrophages by the criteria of increa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.2.383-388.1985
更新日期:1985-08-01 00:00:00
abstract::Previous studies showed that Pseudomonas aeruginosa exotoxin A (PA toxin) catalyzes nicotinamide adenine dinucleotide (NAD)-dependent inhibition of protein synthesis in a rabbit reticulocyte lysate and transfer of radioactivity from [14C]adenine-labeled NAD to a protein having the same molecular weight as elongation f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.138-144.1977
更新日期:1977-01-01 00:00:00
abstract::The ultrastructural appearance of the "halozone" formed at the interface between the spotted fever agent Rickettsia rickettsii and the cytoplasm of persistently infected cultured vole cells (Microtus pennsylvanicus) was studied by transmission electron microscopy. In sections of epoxy-embedded specimens stained with u...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.3.1252-1260.1983
更新日期:1983-09-01 00:00:00
abstract::Adult volunteers received a booster dose (4 x 10(8) CFU) of attenuated Vibrio cholerae CVD 103-HgR oral vaccine 15 or 24 months after primary immunization. The immune response was modest, presumably due to rapid clearance of the vaccine strain by a primed immune system. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.9.3916-3917.1992
更新日期:1992-09-01 00:00:00
abstract::Mycoplasma gallisepticum is an avian respiratory and reproductive tract pathogen that has a significant economic impact on the poultry industry worldwide. Although membrane proteins of Mycoplasma spp. are thought to play crucial roles in host interactions, very few have had their biochemical function defined. In this ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00248-19
更新日期:2019-08-21 00:00:00
abstract::A heterogeneous subset of extraintestinal pathogenic Escherichia coli (ExPEC) strains, referred to as uropathogenic E. coli (UPEC), causes most uncomplicated urinary tract infections. However, no core set of virulence factors exists among UPEC strains. Instead, the focus of the analysis of urovirulence has shifted to ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01608-14
更新日期:2014-09-01 00:00:00
abstract::Endogenous interleukin-12 (IL-12) mediates protection against Yersinia enterocolitica in C57BL/6 mice by triggering gamma interferon (IFN-gamma) production in NK and CD4+ T cells. Administration of exogenous IL-12 confers protection against yersiniae in Yersinia-susceptible BALB/c mice but exacerbates yersiniosis in r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.2213-2220.1998
更新日期:1998-05-01 00:00:00
abstract::We have shown previously that Klebsiella pneumoniae receptors for coliphages T3 and T7 also mediate mannose-inhibitable adherence to human epithelial cells and protect bacteria from phagocytosis and intracellular killing by human polymorphonuclear cells. In this paper we analyze the possible role of such mannose-inhib...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.520-527.1983
更新日期:1983-02-01 00:00:00
abstract::We investigated the ability of two overlapping fragments of staphylococcal enterotoxin B (SEB), which encompass the whole toxin, to induce protection and also examined if passive transfer of chicken anti-SEB antibodies raised against the holotoxin could protect rhesus monkeys against aerosolized SEB. Although both fra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2278-2281.2002
更新日期:2002-05-01 00:00:00
abstract::An enterotoxin produced by Bacteroides fragilis was purified to homogeneity and characterized as to its biological activity and basic molecular properties. Toxin preparations were prepared by growing B. fragilis VPI 13784 in brain heart infusion broth to early stationary phase, immediately precipitating the culture su...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1343-1350.1992
更新日期:1992-04-01 00:00:00
abstract::Streptococcus pneumoniae, a Gram-positive bacterial pathogen, causes pneumonia, meningitis, and septicemia. Innate immune responses are critical for the control and pathology of pneumococcal infections. It has been demonstrated that S. pneumoniae induces the production of type I interferons (IFNs) by host cells and th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01572-14
更新日期:2014-06-01 00:00:00
abstract::Toll-like receptors 2 and 4 (TLR2 and TLR4) have been found to transduce signals of peptidoglycan (PGN) and lipopolysaccharide (LPS), respectively, for NF-kappa B activation. However, little is known about the expression and regulation of the TLR2 gene in monocytes/macrophages in response to the two typical bacterial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.2788-2796.2001
更新日期:2001-05-01 00:00:00
abstract::The effect of route of administration, dosage, and number of boosts employed during immunization with the polymyxin-release form of Escherichia coli heat-labile (LT) enterotoxin on the degree and duration of protection afforded was evaluated in rats which were challenged by the ligated loop technique. Increasing the b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.1.81-86.1980
更新日期:1980-01-01 00:00:00
abstract::Raising the magnesium level from 0.4 to 1.5 mM in a medium containing only amino acids (2.08%), salts, and vitamins increased enterotoxin B production by Staphylococcus aureus S-6 by about 80%. The level of phosphates in the medium was lowered to one-tenth (to 2.87 mM) the original amount without adversely affecting g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.158-160.1978
更新日期:1978-04-01 00:00:00
abstract::Four monoclonal antibodies against Escherichia coli J5 were studied. Each of these monoclonal antibodies reacted with purified lipopolysaccharides from E. coli J5, the deep rough mutant Salmonella minnesota Re595, Agrobacterium tumefaciens, and Pseudomonas aeruginosa PAO1 as well as with the purified lipid A of P. aer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.631-636.1984
更新日期:1984-09-01 00:00:00
abstract::A cytoplasmic 75-kDa immunogen from Chlamydia trachomatis serovar L2 has previously been characterized as being similar to the Escherichia coli heat shock protein DnaK. We have localized a linear epitope for one monoclonal antibody specific for C. trachomatis DnaK. By use of a recombinant DNA technique, the epitope wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.2051-2057.1994
更新日期:1994-05-01 00:00:00
abstract::The disruption of a specific gene in Candida albicans is commonly used to determine the function of the gene product. We disrupted AAF1, a gene of C. albicans that causes Saccharomyces cerevisiae to flocculate and adhere to endothelial cells. We then characterized multiple heterozygous and homozygous mutants. These nu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3193-3198.1999
更新日期:1999-07-01 00:00:00
abstract::The Escherichia coli genome varies in size from 4.5 to 5.5 Mb. It is unclear whether this variation may be distributed finely throughout the genome or is concentrated at just a few chromosomal loci or on plasmids. Further, the functional correlates of size variation in different genome copies are largely unexplored. W...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.1.230-236.1999
更新日期:1999-01-01 00:00:00
abstract::The outcome of coccidioidomycosis depends to a large extent on the effectiveness of the T-cell-mediated immune (CMI) response to the fungal pathogen. For this reason, identification of Coccidioides immitis antigens which stimulate T cells is important for understanding the nature of host defense against the organism a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.424-431.1998
更新日期:1998-02-01 00:00:00
abstract::The antigenic relationship of glucosyltransferases (GTF) produced by different serotypes of Streptococcus mutans was studied by using a functional inhibition assay. Rat, rabbit, or hamster immune fluids, directed to cell-associated or supernatant-derived GTF, were tested against ammonium sulfate-precipitated culture s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.91-103.1977
更新日期:1977-01-01 00:00:00