当前位置： SCI文献检索 > INFECTION AND IMMUNITY期刊下所有文献 > Antigenic relatedness of glucosyltransferase enzymes from streptococcus mutans.

Antigenic relatedness of glucosyltransferase enzymes from streptococcus mutans.

Abstract：

:The antigenic relationship of glucosyltransferases (GTF) produced by different serotypes of Streptococcus mutans was studied by using a functional inhibition assay. Rat, rabbit, or hamster immune fluids, directed to cell-associated or supernatant-derived GTF, were tested against ammonium sulfate-precipitated culture supernatants containing GTF from seven strains of S. mutans representing six different serotypes. An antigenic relationship was shown to exist among GTF from serotypes a, d, and g, since both rat and rabbit antisera directed to serotype a or g GTF inhibited GTF of serotypes d and g similarly and both antisera also inhibited serotype a GTF. Furthermore, serum inhibition patterns indicated that GTF of serotypes c and e, and possibly b, are antigenically related to each other, but are antigenically distinct from GTF of serotype a, d, or g. Serum antibody directed to antigens other than enzyme (e.g., serotype-specific antigen or teichoic acid) had little effect on the inhibition assay. Salivas from rats immunized with cell-associated or supernatant-derived GTF exhibited low but consistent inhibition of GTF activity, which generally corresponded to the serum patterns. The sera of two groups of hamsters immunized with GTF (serotype g), enriched either in water-insoluble or water-soluble glucan synthetic activity, gave patterns of inhibition quite similar to those seen with sera from more heterogenous cell-associated or crude supernatant-derived GTF preparations. Both groups of hamster sera also gave virtually identical patterns, suggesting that the two enzyme forms used as antigen share common antigenic determinants. The results from the three animal models suggest that among the cariogenic organisms tested, two (serotypes a, d, g and b, c, e), or perhaps three (serotypes a, d, g; b; and c, e), different subsets of GTF exist that have distinct antigenic determinants within a subset.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Smith DJ,Taubman MA

doi

10.1128/IAI.15.1.91-103.1977

subject

Has Abstract

pub_date

1977-01-01 00:00:00

pages

91-103

issue

eissn

0019-9567

issn

1098-5522

journal_volume

pub_type

杂志文章

在线工具

Antigenic relatedness of glucosyltransferase enzymes from streptococcus mutans.