Abstract:
:Virulent Treponema pallidum organisms, extracted from infected rabbit testes, were subjected to velocity sedimentation in discontinuous gradients of Hypaque, a high density, low viscosity material. After centrifugation of extracts at 20 C for 45 min at 100,000 x g, treponemes separated into two distinct bands based upon their relative velocities, although some variation was observed in the densities of the two bands and the number of treponemes per band. Rabbit tissue components sedimented more rapidly. Dark field and electron microscopy of preparations after velocity sedimentation indicated that treponemes retained general structural characteristics and no tissue contamination occurred in the treponemal fractions. Purification of treponemes in Hypaque resulted in their loss of motility and infectivity based upon animal inoculation. Antigenicity with respect to reactivity with antibody was preserved as shown by the high fluorescence intensity of treponemes in the fluorescent treponemal antibody adsorption test.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Baseman JB,Nichols JC,Rumpp JW,Hayes NSdoi
10.1128/IAI.10.5.1062-1067.1974keywords:
subject
Has Abstractpub_date
1974-11-01 00:00:00pages
1062-7issue
5eissn
0019-9567issn
1098-5522journal_volume
10pub_type
杂志文章abstract::The endothelium is a specific target for Bartonella henselae, and endothelial cell infection represents an important step in the pathogenesis of cat scratch disease and bacillary angiomatosis. Mechanisms of Bartonella-endothelial cell interaction as well as signaling pathways involved in target cell activation were an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.5088-5097.2001
更新日期:2001-08-01 00:00:00
abstract::Patent filarial infection is associated with the downregulation of parasite-specific immune reactivity. In the present study, the relationship between in vitro parasite antigen-specific humoral and cellular immune responsiveness was investigated in Brugia pahangi-infected jirds and in jirds immunized with soluble anti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3052-3057.1988
更新日期:1988-12-01 00:00:00
abstract::Slime production is not a generally recognized feature of Staphylococcus epidermidis. In a recent outbreak of S. epidermidis intravascular catheter-associated sepsis, we noted that 63% of clinically implicated strains grew as a slimy film coating the culture tube walls when propagated in tryptic soy broth. Only 37% of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.318-326.1982
更新日期:1982-07-01 00:00:00
abstract::Haemophilus influenzae type b (Hib) is a significant pathogen for young children, and three Hib vaccines (named PRP-OMPC, HbOC, and PRP-T) are currently available for young children. Extensive studies of anti-Hib polysaccharide (PS) antibodies (Abs) have shown that the V regions of Abs against the Hib PS comprise a VH...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.11.4219-4223.1995
更新日期:1995-11-01 00:00:00
abstract::Mycoplasma arthritidis induces an acute to chronic arthritis in rodents. Arthritis induced in mice histologically resembles human rheumatoid arthritis and can be associated with lethal toxicity following systemic injection. The M. arthritidis mitogen (MAM) superantigen has long been implicated as having a role in path...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00667-08
更新日期:2008-11-01 00:00:00
abstract::Binding of Escherichia coli strain 431 heat-stable enterotoxin (STa) and activation of intestinal particulate guanylate cyclase by E. coli STa were studied with rat intestinal epithelial cells and brush border membranes (BBMs). The rates of guanylate cyclase stimulation by 431 STa in cells and BBMs were rapid, with ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.537-543.1984
更新日期:1984-11-01 00:00:00
abstract::Haemophilus ducreyi expresses 2 OmpA homologs, designated MOMP and OmpA2, whose genes are arranged in tandem on the chromosome. Northern blot analysis indicated that momp and ompA2 are transcribed independently. Sequences of the momp open reading frame (ORF) lacking the transcriptional start site were amplified by PCR...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2602-2607.2000
更新日期:2000-05-01 00:00:00
abstract::Helicobacter mustelae is a urease-rich bacterium associated with gastritis in ferrets. The ureases of H. mustelae and Helicobacter pylori, a bacterium implicated in human gastritis, share many characteristics. Helicobacter sp. ureases appear to be unique among bacterial enzymes in exhibiting submillimolar Km values an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.3343-3345.1991
更新日期:1991-09-01 00:00:00
abstract::The suppression of T-cell deoxyribonucleic acid (DNA) synthesis by serum from mice acutely with murine cytomegalovirus (MCMV) was investigated. Spleen cells from uninfected mice were exposed to concanavalin A in the presence of serum taken from mice at various times after infection with MCMV. The capacity of the serum...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.2.378-381.1977
更新日期:1977-08-01 00:00:00
abstract::Adherence of erythrocytes infected with Plasmodium falciparum to microvascular endothelial cells (sequestration) is considered to play an important role in parasite virulence and pathogenesis. However, the real importance of sequestration for infection and disease has never been fully assessed. The absence of an appro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4135-4144.2000
更新日期:2000-07-01 00:00:00
abstract::A Mycobacterium tuberculosis strain disrupted in the AraC homologue Rv1931c was isolated. The mutant strain exhibited reduced survival both in macrophages and in a mouse infection model, with survival being restored on complementation with the Rv1931c gene. These results suggest that Rv1931c regulates genes important ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5483-5486.2004
更新日期:2004-09-01 00:00:00
abstract::ExoU, a phospholipase injected into host cells by the type III secretion system of Pseudomonas aeruginosa, leads to rapid cytolytic cell death. Although the importance of ExoU in infection is well established, the mechanism by which this toxin kills host cells is less clear. To gain insight into how ExoU causes cell d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.2552-2561.2006
更新日期:2006-05-01 00:00:00
abstract::Bordetella parapertussis, a respiratory tract pathogen commonly regarded as noninvasive, was found to invade HeLa 229 cell monolayers. Following treatment of the monolayers with gentamicin, numbers of viable B. parapertussis recovered were comparable to those of invasive Salmonella and Shigella isolates. Invasion occu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.4.1240-1247.1989
更新日期:1989-04-01 00:00:00
abstract::Borrelia burgdorferi, a tick-borne bacterial pathogen, causes a disseminated infection involving multiple organs known as Lyme disease. Surface proteins can directly participate in microbial virulence by facilitating pathogen dissemination via interaction with host factors. We show here that a fraction of the B. burgd...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05671-11
更新日期:2012-01-01 00:00:00
abstract::The immune response of humans and mice to temperature-specific, plasmid- or chromosome-encoded proteins of yersinia pestis and Yersinia enterocolitica was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. Extracts from Y. pestis and Y. enterocolitica strains with and witho...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.676-685.1985
更新日期:1985-06-01 00:00:00
abstract::Vibrio cholerae accessory colonization factor genes (acfA, B, C, and D) are required for efficient intestinal colonization. Expression of acf genes is under the control of a regulatory cascade that also directs the synthesis of cholera toxin and proteins involved in the biogenesis of the toxin-coregulated pilus. The g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3289-3298.1994
更新日期:1994-08-01 00:00:00
abstract::Antimicrobial peptides, such as defensins or cathelicidins, are effector substances of the innate immune system and are thought to have antimicrobial properties that contribute to host defense. The evidence that vertebrate antimicrobial peptides contribute to innate immunity in vivo is based on their expression patter...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.6084-6089.1999
更新日期:1999-11-01 00:00:00
abstract::Immune and pathologic responses were measured for 20 weeks after infection of mice with Brucella abortus 19, RB51, or 2308. Live bacteria and bacterial antigens of 19 and RB51 persisted in spleens for 10 and 4 weeks after infection, respectively, whereas 2308 bacteria and bacterial antigens persisted for at least 20 w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3206-3212.1994
更新日期:1994-08-01 00:00:00
abstract::The mechanism by which bacterial heat-stable enterotoxins (ST I STA) cause diarrhea in humans and animals has been linked to the activation of an intestinal membrane-bound guanylate cyclase. Guanylin, a recently discovered rat intestinal peptide, is homologous in structure to ST I and can activate guanylate cyclase pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4710-4715.1993
更新日期:1993-11-01 00:00:00
abstract::Tissues and organs provide the structural and biochemical landscapes upon which microbial pathogens and commensals function to regulate health and disease. While flat two-dimensional (2-D) monolayers composed of a single cell type have provided important insight into understanding host-pathogen interactions and infect...
journal_title:Infection and immunity
pub_type: 历史文章,杂志文章,评审
doi:10.1128/IAI.00282-18
更新日期:2018-10-25 00:00:00
abstract::Sterile sonic-treated materials of pneumococci grown in media containing N-acetylmannosamine as the carbohydrate source were lethal for mice. Purification of the toxic factor led to its identification as the enzyme neuraminidase. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.1.115-117.1970
更新日期:1970-07-01 00:00:00
abstract::The Plasmodium falciparum liver-stage antigen 3 (LSA3), a recently identified preerythrocytic antigen, induces protection against malaria in chimpanzees. Using antibodies from individuals with hyperimmunity to malaria affinity purified on recombinant or synthetic polypeptides of LSA3, we identified four non-cross-reac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3845-3952.2001
更新日期:2001-06-01 00:00:00
abstract::The Pseudomonas aeruginosa quorum-sensing systems, las and rhl, control the production of numerous virulence factors. In this study, we have used the burned-mouse model to examine the contribution of quorum-sensing systems to the pathogenesis of P. aeruginosa infections in burn wounds. Different quorum-sensing mutants...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.5854-5862.1999
更新日期:1999-11-01 00:00:00
abstract::Culture supernatants from antigen-pulsed spleen cells of mice infected previously with either BCG or Plasmodium chabaudi were used to study macrophage activation as judged by phagocytosis of immunoglobulin G-sensitized erythrocytes and Plasmodium berghei- and P. chabaudi-infected erythrocytes. Resident peritoneal macr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.2.733-739.1983
更新日期:1983-11-01 00:00:00
abstract::An apparent inhibition of induction of mouse hepatic tryptophan oxygenase by endotoxin has been reported previously, as evidenced by low catalytic activity. This could be due either to decreased tryptophan oxygenase levels or to inactivation of existing enzyme molecules. To resolve this question, the enzyme was quanti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.4.534-539.1973
更新日期:1973-10-01 00:00:00
abstract::Rinderpest virus infection was shown to induce marked suppression of both humoral antibody response and cell-mediated immunity in rabbits. The virus exhibited a suppressive effect on primary antibody response as indicated by a decrease in numbers of plaque-forming cells (immunoglobulin [Ig]M) and hemagglutinating anti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.2.206-211.1974
更新日期:1974-02-01 00:00:00
abstract::Ehrlichia chaffeensis, an obligatory intracellular bacterium of monocytes or macrophages, is the etiologic agent of human monocytic ehrlichiosis. Our previous study showed that gamma interferon (IFN-gamma) added prior to or at early stage of infection inhibited infection of human monocytes with E. chaffeensis; however...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2514-2520.1998
更新日期:1998-06-01 00:00:00
abstract::The leading malaria vaccine candidate, RTS,S, based on the Plasmodium falciparum circumsporozoite protein (CSP), will likely be the first publicly adopted malaria vaccine. However, this and other subunit vaccines, such as virus-vectored thrombospondin-related adhesive protein (TRAP), provide only intermediate to low l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01063-15
更新日期:2015-12-14 00:00:00
abstract::Conjunctival infection of guinea pigs by the chlamydial agent of guinea pig inclusion conjunctivitis confers immunity. However, the mechanism of resistance to this intracellular pathogen is not yet defined. In the study reported here, serum immunoglobulin was passively transferred with resultant titers in excess of th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.4.597-599.1973
更新日期:1973-04-01 00:00:00
abstract::Various features of salmonellosis were examined in a burned-mouse model. In this model, which uses an outbred mouse strain, a challenge dose of ca. 100 CFU with any of several strains of Salmonella typhimurium caused a fatal infection. A variety of mutated strains attenuated for virulence in Salmonella-susceptible par...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.9.2842-2846.1989
更新日期:1989-09-01 00:00:00