Abstract:
:Bordetella parapertussis, a respiratory tract pathogen commonly regarded as noninvasive, was found to invade HeLa 229 cell monolayers. Following treatment of the monolayers with gentamicin, numbers of viable B. parapertussis recovered were comparable to those of invasive Salmonella and Shigella isolates. Invasion occurs through a cytochalasin-sensitive process which appears to be distinct from receptor-mediated endocytosis. Hyperimmune antisera raised against filaments hemagglutinin, a major adhesion of B. pertussis, did not inhibit invasion by B. parapertussis, suggesting that alternate adhesin(s) are required for invasion. In addition, B. parapertussis was found to invade human respiratory epithelial cells in primary culture, as demonstrated in ultrathin sections viewed by transmission electron microscopy. Although viable intracellular B. parapertussis persist within HeLa cells, they do not multiply there and the monolayers remain intact, suggesting a possible mechanism of carriage for these organisms.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Ewanowich CA,Sherburne RK,Man SF,Peppler MSdoi
10.1128/IAI.57.4.1240-1247.1989subject
Has Abstractpub_date
1989-04-01 00:00:00pages
1240-7issue
4eissn
0019-9567issn
1098-5522journal_volume
57pub_type
杂志文章abstract::The leading malaria vaccine candidate, RTS,S, based on the Plasmodium falciparum circumsporozoite protein (CSP), will likely be the first publicly adopted malaria vaccine. However, this and other subunit vaccines, such as virus-vectored thrombospondin-related adhesive protein (TRAP), provide only intermediate to low l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01063-15
更新日期:2015-12-14 00:00:00
abstract::Secretion of immunoglobulin A1 (IgA1) proteases is a characteristic of Haemophilus influenzae and several other bacterial pathogens causing infectious diseases, including meningitis. Indirect evidence suggests that the proteases are important virulence factors. In this study, we cloned the iga gene encoding immunoglob...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.3097-3105.1989
更新日期:1989-10-01 00:00:00
abstract::The role of the spleen in hepatic amoebiasis in hamsters was studied. In hamsters receiving an intrahepatic inoculation of 10(5) trophozoites of axenic Entamoeba histolytica at 7 or 14 days postsplenectomy, the mean weight of metastatic foci increased significantly when compared with sham-splenectomized or intact cont...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.571-573.1981
更新日期:1981-02-01 00:00:00
abstract::The type III secretion system (TTSS) of Pseudomonas aeruginosa is induced in vivo upon contact with eukaryotic cells and in vitro by calcium depletion in culture medium. We have observed a previously identified protein, PsrA, necessary for full activation of TTSS gene expression in P. aeruginosa. Electrophoretic mobil...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.1121-1129.2006
更新日期:2006-02-01 00:00:00
abstract::Enterotoxigenic Escherichia coli (ETEC) is a major cause of traveler's diarrhea as well as of endemic diarrhea and stunting in children in developing areas. However, a small-mammal model has been badly needed to better understand and assess mechanisms, vaccines, and interventions. We report a murine model of ETEC diar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00183-18
更新日期:2018-06-21 00:00:00
abstract::Candida albicans is a major opportunistic pathogen in immunocompromised patients. Production of proinflammatory cytokines by host cells in response to C. albicans plays a critical role in the activation of immune cells and final clearance of the organism. Invasion of host cells and tissues is considered one of the vir...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.4588-4595.2005
更新日期:2005-08-01 00:00:00
abstract::Toxoplasma gondii is a ubiquitous parasite that persists for the life of a healthy mammalian host. A latent, chronic infection can reactivate upon immunosuppression and cause life-threatening diseases, such as encephalitis. A key to the pathogenesis is the parasite's interconversion between the tachyzoite (in acute in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01862-06
更新日期:2007-04-01 00:00:00
abstract::Borrelia burgdorferi, the Lyme disease pathogen, dramatically alters its protein profile when it is transmitted between ticks and mammals. Several differentially expressed proteins have been shown to be critical for the enzootic cycle of B. burgdorferi. In this study, we demonstrated that expression of the surface lip...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00372-10
更新日期:2010-07-01 00:00:00
abstract::Mannan (MAN) extracted from Candida albicans 20A was investigated for its potential as an antigen in the detection of cell-mediated immunity (CMI) in vivo and in vitro and for its ability to modulate CMI when administered intravenously (i.v.). CBA/J mice were either immunized as adults by the cutaneous inoculation of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.693-700.1989
更新日期:1989-03-01 00:00:00
abstract::The surface antigen makeup of metacyclic trypomastigote forms of strain G of Trypanosoma cruzi, which produce a subpatent infection in mice, differed from those of the virulent strains Y and CL. A 100,000-molecular-weight protein, barely detectable on the Y or CL cell surface, appeared as the main surface antigen of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.836-839.1983
更新日期:1983-05-01 00:00:00
abstract::Shiga toxin type 1 (Stx1) belongs to the Shiga family of bipartite AB toxins that inactivate eukaryotic 60S ribosomes. The A subunit of Stxs are N-glycosidases that share structural and functional features in their catalytic center and in an internal hydrophobic region that shows strong transmembrane propensity. Both ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5252-5259.1998
更新日期:1998-11-01 00:00:00
abstract::The study of how bacteria respond to and obtain divalent metal ions provides insight into the regulation of virulence factors in the host environment. Regulation of metal permease operons in gram-positive bacteria may involve the binding of metal-responsive repressors to palindromic domains in their control regions. T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.10.5706-5714.2002
更新日期:2002-10-01 00:00:00
abstract::A persistent Staphylococcus epidermidis infection in mice around a subcutaneous polyvinylpyrrolidone-grafted silicon elastomer catheter (SEpvp) but not around a conventional silicon elastomer catheter was observed. With SEpvp pericatheter tissue, protracted and exaggerated interleukin-1beta (IL-1beta) production was f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1692-1695.2000
更新日期:2000-03-01 00:00:00
abstract::Exposure of hamster tracheal rings in organ culture to virulent Mycoplasma pneumoniae organisms leads to alterations in macromolecular biosynthesis and metabolic activity of the respiratory epithelial cells. Avirulent organisms derived from the same parent strain do not produce these effects. During the course of infe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.4.704-710.1975
更新日期:1975-04-01 00:00:00
abstract::Liver-stage antigen 1 (LSA-1) is a potential vaccine candidate against preerythrocytic stages of malaria. We report here the immunogenicity of linear synthetic constructs delineated as T(H)-cell determinants from the nonrepeat regions of Plasmodium falciparum LSA-1 in murine models and human subjects from areas where ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.1.141-150.2000
更新日期:2000-01-01 00:00:00
abstract::The nonacylated form of protein D (PDm) of Haemophilus influenzae has been shown to induce the production of antibodies that are bactericidal to homologous and heterologous nontypeable H. influenzae (NTHi) strains. In this study, immunization of rats with lipoprotein D (LPD) induced higher levels of anti-protein D imm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.5010-5016.1997
更新日期:1997-12-01 00:00:00
abstract::Archived sera (collected in 1946) from acute rheumatic fever (ARF) and untreated scarlet fever and/or pharyngitis patients were reacted with streptococcal M protein, cardiac myosin, and cardiac tropomyosin. Except for very low levels to tropomyosin, antibodies to other antigens were not elevated in the sera of ARF pat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.7132-7136.2000
更新日期:2000-12-01 00:00:00
abstract::The cytolethal distending toxin (Cdt) of Actinobacillus actinomycetemcomitans is an atypical A-B-type toxin consisting of a heterotrimer composed of the cdtA, cdtB, and cdtC gene products. The CdtA and CdtC subunits form two heterogeneous ricin-like lectin domains which bind the holotoxin to the target cell. Point mut...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00697-06
更新日期:2006-09-01 00:00:00
abstract::The plasma level of the chemokine CCL3 is elevated in patients with chronic severe schistosomiasis mansoni. We have previously shown that CCL3(-/-) mice with experimental infection showed diminished pathology and worm burden compared to those of wild-type (WT) mice. To elucidate further the role of CC chemokines durin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00502-10
更新日期:2011-04-01 00:00:00
abstract::Studies were carried out to determine whether the in vitro capacity of adherent peritoneal cells to replicate herpes simplex virus type 1 (HSV-1) might correlate with the in vivo susceptibility of mice genetically resistant, moderately susceptible, or very susceptible to HSV-1 infection. Unstimulated and proteose pept...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.2.432-437.1979
更新日期:1979-02-01 00:00:00
abstract::Vaccines for P. falciparum will need to contain both T- and B-cell epitopes. Conserved epitopes are the most desirable, but they are often poorly immunogenic. The major merozoite surface protein 1 (MSP-1) is currently a leading vaccine candidate antigen. In this study, six peptides from conserved or partly conserved r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2685-2691.2000
更新日期:2000-05-01 00:00:00
abstract::Infection by Shigella flexneri is characterized by infiltration of neutrophils in the intestinal mucosa and by a strong inflammatory reaction. Although neutrophils are constitutively programmed to die by apoptosis, we show that isolated human neutrophils undergo necrosis 2 h after infection with virulent S. flexneri s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1289-1296.2000
更新日期:2000-03-01 00:00:00
abstract::We performed ex vivo experiments with Plasmodium falciparum-infected human placentas from primi- and multigravida women from Cameroon. All women, independent of their gravida status, had anti-chondroitin sulfate A (CSA) adhesion antibodies which cross-reacted with heterologous strains, such as FCR3 and Palo-Alto(FUP)1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Science has a critical role to play in addressing humanity's most important challenges in the twenty-first century. However, the contemporary scientific enterprise has developed in ways that prevent it from reaching maximum effectiveness and detract from the appeal of a research career. To be effective, the methodolog...
journal_title:Infection and immunity
pub_type: 社论
doi:10.1128/IAI.06184-11
更新日期:2012-03-01 00:00:00
abstract::The toxicity by inhalation of various gram-negative bacteria, isolated from settings associated with inhalation disease, was studied by a variety of means. These microorganisms were not equally toxic. Citrobacter freundii aerosol challenges of rabbits provoked significant (up to fivefold) increases in plasma haptoglob...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.133-138.1983
更新日期:1983-04-01 00:00:00
abstract::Pathogenic strains of Yersinia spp. inject a set of Yop effector proteins into eukaryotic cells by using a plasmid-encoded type III secretion system. In this study, we analyzed the inflammatory response of human umbilical vein endothelial cells (HUVECs) after infection with different Yersinia enterocolitica strains. W...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3510-3520.2002
更新日期:2002-07-01 00:00:00
abstract::Interleukin-10 (IL-10)-producing B cells (B10 cells) play a critical role in the immune system balance by negatively regulating inflammatory responses. This study was conducted to determine the effect of local B10 cell induction on periodontal inflammation and bone loss in ligature-induced experimental periodontitis i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00645-16
更新日期:2016-12-29 00:00:00
abstract::The presence of hepatitis B surface antigen (HBsAg) and antibody (anti-HBs), hepatitis B e antigen (HBeAg) and antibody (anti-HBe), the nature of T-cell function, and specific cell-mediated immunity to HBsAg were determined and evaluated serially in groups of subjects with chronic HBsAg carrier states and in seronegat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.480-488.1978
更新日期:1978-08-01 00:00:00
abstract::The protective efficacy of antibodies to the Staphylococcus aureus type 5 capsular polysaccharide (CP5) was examined in a modified model of catheter-induced endocarditis. Rats were catheterized by surgically passing a polyethylene catheter through the right carotid artery and aortic valve into the left ventricle. The ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4146-4151.1997
更新日期:1997-10-01 00:00:00
abstract::Burkholderia cenocepacia, a member of the B. cepacia complex, is an opportunistic pathogen that causes serious infections in patients with cystic fibrosis. We identified a six-gene cluster in chromosome 1 encoding a two-component regulatory system (BCAL2831 and BCAL2830) and an HtrA protease (BCAL2829) hypothesized to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01581-06
更新日期:2007-04-01 00:00:00