Abstract:
:The toxicity by inhalation of various gram-negative bacteria, isolated from settings associated with inhalation disease, was studied by a variety of means. These microorganisms were not equally toxic. Citrobacter freundii aerosol challenges of rabbits provoked significant (up to fivefold) increases in plasma haptoglobin 24 to 48 h after inhalation. Other strains tested failed to provoke such statistically consistent increases. Measurements of C-reactive protein in these same animals did not lead to as reliable results, due to the variability of the responses. Mice responded differently to inhalation in that haptoglobin responses were either unaffected or depressed. When a strain of mice was used that exhibits more severe inflammatory responses to endotoxin (C3H/HeJ), C. freundii and Escherichia coli aerosols provoked significant haptoglobin increases. Free lung cell analyses demonstrated that the macrophage and neutrophil responses differed depending on the strain of bacteria used. Again, C. freundii induced the greatest responses. When murine B lymphocytes were stimulated with lipopolysaccharide preparations from different gram-negative bacteria, distinctly different dose-response curves were obtained. The types of responses obtained indicate that (i) brief inhalation of bacterial aerosols previously thought to be innocuous may lead to pulmonary inflammation, and (ii) that these bacteria differ in their toxicity, with C. freundii being the most toxic organism of the five studied.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Baseler MW,Fogelmark B,Burrell Rdoi
10.1128/IAI.40.1.133-138.1983subject
Has Abstractpub_date
1983-04-01 00:00:00pages
133-8issue
1eissn
0019-9567issn
1098-5522journal_volume
40pub_type
杂志文章abstract::The leading malaria vaccine candidate, RTS,S, based on the Plasmodium falciparum circumsporozoite protein (CSP), will likely be the first publicly adopted malaria vaccine. However, this and other subunit vaccines, such as virus-vectored thrombospondin-related adhesive protein (TRAP), provide only intermediate to low l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01063-15
更新日期:2015-12-14 00:00:00
abstract::An attenuated Salmonella typhi Ty21a vaccine was administered to 18 infants and toddlers (less than or equal to 24 months old) to determine its safety and immunogenicity. The vaccination (10(9) CFU per dose, three doses) was well tolerated. However, after the vaccination there was no evidence of a humoral or cellular ...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.59.11.4291-4293.1991
更新日期:1991-11-01 00:00:00
abstract::Bartonella henselae is a recently recognized pathogenic bacterium associated with cat scratch disease, bacillary angiomatosis, and bacillary peliosis. This study describes the cloning, sequencing, and characterization of an antigenic autotransporter gene from B. henselae. A cloned 6.0-kb BclI-EcoRI DNA fragment expres...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00533-07
更新日期:2007-11-01 00:00:00
abstract::The serum antibody response to proteins encoded by the virulence-associated plasmid of Shigella flexneri was determined in monkeys challenged with virulent S. flexneri serotype 2a. With water-extractable antigen in an enzyme-linked immunosorbent assay, a significant increase in antibody titer against proteins from a p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.1.57-63.1986
更新日期:1986-07-01 00:00:00
abstract::Studies have implicated Gardnerella vaginalis as an important etiological agent in bacterial vaginosis (BV). It produces a cholesterol-dependent cytolysin, vaginolysin (VLY). In this study, we sought to characterize the interaction between vaginal epithelium, G. vaginalis, and VLY using EpiVaginal tissues from MatTek....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00646-18
更新日期:2019-03-25 00:00:00
abstract::A purified human granulocyte colony-stimulating factor (hG-CSF) was studied for its protective effect on the induction of neutropenia and enhanced susceptibility to microbial infections in mice receiving cyclophosphamide (CPA). A severe reduction in peripheral blood neutrophils was induced 4 days after injection with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.11.2715-2720.1987
更新日期:1987-11-01 00:00:00
abstract::Rinderpest virus infection was shown to induce marked suppression of both humoral antibody response and cell-mediated immunity in rabbits. The virus exhibited a suppressive effect on primary antibody response as indicated by a decrease in numbers of plaque-forming cells (immunoglobulin [Ig]M) and hemagglutinating anti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.2.206-211.1974
更新日期:1974-02-01 00:00:00
abstract::In its canonical role the reverse transcriptase telomerase recovers the telomeric repeats that are lost during DNA replication. Other locations and activities have been recently described for the telomerase protein subunit TERT in mammalian cells. In the present work, using biochemistry, molecular biology, and electro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02269-14
更新日期:2015-01-01 00:00:00
abstract::Burkholderia cepacia is an opportunistic pathogen that causes severe systemic infections in patients with chronic granulomatous disease (CGD) or with cystic fibrosis (CF), but its mechanisms of virulence are poorly understood. We developed a murine model of systemic infection in wild-type (WT) and gamma interferon kno...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.4027-4032.1999
更新日期:1999-08-01 00:00:00
abstract::Although protective immunity in C57BL/6 mice induced by a single dose of the radiation-attenuated schistosome vaccine is believed to be mediated by Th1-type immune responses, we here report that in BALB/c mice protection can also depend upon signaling via the interleukin-4 (IL-4) receptor which conventionally governs ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.228-236.2001
更新日期:2001-01-01 00:00:00
abstract::Optimal protective immunity against babesial infection is postulated to require both complement-fixing and opsonizing antibodies in addition to gamma interferon (IFN-gamma)-mediated macrophage activation. The rhoptry-associated protein 1 (RAP-1) of Babesia bigemina induces partial protective immunity and is a candidat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.1.155-164.1999
更新日期:1999-01-01 00:00:00
abstract::Exposure to bacterial superantigens such as staphylococcal enterotoxin B (SEB) leads to the induction of toxic shock syndrome which results in multiorgan failure, including liver damage. In the present study, we investigated the role of CD44 in SEB-induced liver injury. Injection of SEB into d-galactosamine-sensitized...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.50-61.2005
更新日期:2005-01-01 00:00:00
abstract::Serotypes A and B of the relapsing fever spirochete Borrelia turicatae produce different disease manifestations in infected mice. Whereas serotype B causes more severe arthritis and reaches higher densities in the blood of mice than serotype A, serotype A invades the central nervous system earlier than serotype B duri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4637-4645.1999
更新日期:1999-09-01 00:00:00
abstract::Neisseria lactamica, a common human pharyngeal commensal, contributes to acquired immunity to Neisseria meningitidis. To define the surface antigens shared between these two species, we used monoclonal antibodies (MAbs) to study 35 N. lactamica strains isolated in various parts of the world for cross-reactivity with m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.2.602-608.1989
更新日期:1989-02-01 00:00:00
abstract::Aspergillus species are saprophytic molds causing life-threatening invasive fungal infections in the immunocompromised host. Innate immune recognition, in particular, the mechanisms of opsonization and complement activation, has been reported to be an integral part of the defense against fungi. We have shown that the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00032-13
更新日期:2013-05-01 00:00:00
abstract::Recent work has demonstrated that the polyketide natural product Aurodox from Streptomyces goldiniensis is able to block the pathogenesis of the murine pathogen Citrobacter rodentium In this work, we aimed to gain a better understanding of the mechanism of action of the compound. We show that Aurodox downregulates the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00595-18
更新日期:2019-01-24 00:00:00
abstract::The biological response to endotoxin mediated through the Toll-like receptor 4 (TLR4)-MD-2 receptor complex is directly related to lipid A structure or configuration. Endotoxin structure may also influence activation of the MyD88-dependent and -independent signaling pathways of TLR4. To address this possibility, human...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.2940-2950.2005
更新日期:2005-05-01 00:00:00
abstract::We have studied polyacryl starch microparticles as an adjuvant in oral vaccination in mice. Secreted antigens from Salmonella enterica serovar Enteritidis were administered covalently conjugated to microparticles, or as free antigens, orally or intramuscularly and evaluated for their immunogenicity and ability to elic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1434-1442.2002
更新日期:2002-03-01 00:00:00
abstract::Ehrlichia chaffeensis, a tick-transmitted rickettsial, is the causative agent of human monocytic ehrlichiosis. To examine protein expression patterns, we analyzed total, membrane, and immunogenic proteomes of E. chaffeensis originating from macrophage and tick cell cultures. Total proteins resolved by one-dimensional ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00484-08
更新日期:2008-11-01 00:00:00
abstract::We have cloned and sequenced the d15 gene from two strains of Haemophilus influenzae type b (Hib) and two strains of nontypeable H. influenzae (NTHI). The nucleotide and deduced protein sequences of d15 are highly conserved, with only a small variable region identified near the carboxyl terminus of the protein. Analys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3701-3707.1997
更新日期:1997-09-01 00:00:00
abstract::The effects of 1,25-dihydroxy vitamin D3 and gamma interferon on the phenotypic changes associated with monocyte maturation in vitro were investigated. Human monocytes separated from peripheral blood mononuclear cell populations by adherence to plastic were cultured for 7 days on glass. Immunocytological analysis was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2017-2020.1987
更新日期:1987-09-01 00:00:00
abstract::A previously undescribed, immunoreactive, membranous spherule outer wall (SOW) fraction produced by Coccidioides immitis (strains 634 and 735) grown in culture was isolated. Both this fraction and intact spherules were reactive with sera from coccidioidomycosis patients, as demonstrated by immunofluorescence microscop...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2686-2694.1988
更新日期:1988-10-01 00:00:00
abstract::Previous studies have suggested that interleukin-4 (IL-4) has a protective effect in host defense to Borrelia burgdorferi infection, both in limiting the severity of arthritis and in controlling spirochete numbers in tissues, and a mapping study revealed suggestive linkage to a cluster of genes on mouse chromosome 11,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.10.5603-5609.2000
更新日期:2000-10-01 00:00:00
abstract::Lipopolysaccharide (LPS) is one of the most potent trigger substances for monocytes and macrophages causing secretion of tumor necrosis factor alpha (TNF-alpha) and other inflammatory mediators. The nature of the nuclear factors involved in human TNF-alpha gene regulation is still unknown. Nuclear factor kappa B (NF-k...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1529-1534.1995
更新日期:1995-04-01 00:00:00
abstract::Streptococcus sanguis 804 (NCTC 10904) was grown ih batch culture at constant pH. and the glucosyltransferase activity of the supernatant was assayed over a 40-h growth period. The optimum pH for enzyme production was 7.0 to 7.2. During growth of the culture, three reproducible phases of enzyme activity were observed....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.50-58.1977
更新日期:1977-01-01 00:00:00
abstract::Levels of the serum opsonin mannan-binding lectin (MBL) were directly correlated with the probability of developing visceral leishmaniasis. Monocytes infected with MBL-opsonized Leishmania chagasi promastigotes secreted higher levels of tumor necrosis factor alpha and interleukin-6 than cells infected with nonopsonize...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.5212-5215.2001
更新日期:2001-08-01 00:00:00
abstract::Polymicrobial intra-abdominal infections (IAI) are clinically prevalent and cause significant morbidity and mortality, especially those involving fungi. Our laboratory developed a mouse model of polymicrobial IAI and demonstrated that coinfection with Candida albicans and Staphylococcus aureus (C. albicans/S. aureus) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00348-19
更新日期:2019-07-23 00:00:00
abstract::When phagocytosis of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) takes place on the surface of cultured human endothelial cells, the endothelial monolayers are damaged by lysosomal enzymes that are released by the PMN. Because PMN can phagocytose opsonized as well as unopsonized staphylococci on ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1455-1460.1987
更新日期:1987-06-01 00:00:00
abstract::The gene expression and cytokine release of the proinflammatory cytokines interleukin-1 beta (IL-1 beta), IL-6, and tumor necrosis factor alpha (TNF-alpha) after infection of human epithelial cells (HEp-2 cells) and polymorphonuclear granulocytes (PMNs) were investigated by using isogenic pairs of Listeria monocytogen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2545-2552.1993
更新日期:1993-06-01 00:00:00
abstract::Production of group A streptococcal pyrogenic exotoxins (SPE) type A and C was transferred from toxigenic streptococcal strains to nontoxigenic strains by lysogeny. Lysogens were tested for SPE with Ouchterlony immunodiffusion on Todd-Hewitt agar plates; toxin diffusing from isolated colonies reacted with specific hyp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.28.1.254-257.1980
更新日期:1980-04-01 00:00:00