Abstract:
:Ehrlichia chaffeensis, a tick-transmitted rickettsial, is the causative agent of human monocytic ehrlichiosis. To examine protein expression patterns, we analyzed total, membrane, and immunogenic proteomes of E. chaffeensis originating from macrophage and tick cell cultures. Total proteins resolved by one-dimensional gel electrophoresis and subjected to liquid chromatography-electrospray ionization ion trap mass spectrometry allowed identification of 134 and 116 proteins from macrophage- and tick cell-derived E. chaffeensis, respectively. Because a majority of immunogenic proteins remained in the membrane fraction, individually picked total and immunogenic membrane proteins were also surveyed by liquid chromatography-tandem mass spectrometry and matrix-assisted laser desorption ionization-time of flight methods. The analysis aided the identification of 48 additional proteins. In all, 278 genes of the E. chaffeensis genome were verified as functional genes. They included genes for DNA and protein metabolism, energy metabolism and transport, membrane proteins, hypothetical proteins, and many novel proteins of unknown function. The data reported in this study suggest that the membrane of E. chaffeensis is very complex, having many expressed proteins. This study represents the first and the most comprehensive analysis of E. chaffeensis-expressed proteins. This also is the first study confirming the expression of nearly one-fourth of all predicted genes of the E. chaffeensis genome, validating that they are functionally active genes, and demonstrating that classic shotgun proteomic approaches are feasible for tick-transmitted intraphagosomal bacteria. The identity of novel expressed proteins reported in this study, including the large selection of membrane and immunogenic proteins, will be valuable in elucidating pathogenic mechanisms and developing effective prevention and control methods.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Seo GM,Cheng C,Tomich J,Ganta RRdoi
10.1128/IAI.00484-08subject
Has Abstractpub_date
2008-11-01 00:00:00pages
4823-32issue
11eissn
0019-9567issn
1098-5522pii
IAI.00484-08journal_volume
76pub_type
杂志文章abstract::The ScpC protease of Streptococcus pyogenes degrades interleukin-8 (IL-8), a chemokine that mediates neutrophil transmigration and activation. The ability to degrade IL-8 differs dramatically among clinical isolates of S. pyogenes. Bacteria expressing ScpC overcome immune clearance by preventing the recruitment of neu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00128-08
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.65.8.3165-3170.1997
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journal_title:Infection and immunity
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doi:10.1128/IAI.40.2.534-541.1983
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.9.5.843-850.1974
更新日期:1974-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1047-1053.1984
更新日期:1984-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.2.502-508.1979
更新日期:1979-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.5.728-733.1972
更新日期:1972-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5225-5232.1996
更新日期:1996-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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doi:10.1128/IAI.3.6.756-761.1971
更新日期:1971-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.1827-1833.2000
更新日期:2000-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1899-1905.1995
更新日期:1995-05-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1971-02-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2007-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2013-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2019-07-23 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1997-01-01 00:00:00