Abstract:
:Capsular glucuronoxylomannans (GXM) of Cryptococcus neoformans var. neoformans isolates from patients with recurrent cryptococcal meningitis were analyzed by 1H nuclear magnetic resonance spectroscopy and for reactivity with factor sera (Iatron, Tokyo, Japan). For each patient the initial and relapse isolates had previously been shown to be indistinguishable by DNA restriction fragment length polymorphism analysis. For patients J11 and J22 the GXM of the initial and relapse isolates were identical. For patients SB4 and SB6 the GXM of the initial and relapse isolates differed in structure and reactivity with factor sera. In patient SB4 the initial isolate had a serotype A/D structure, and the first relapse isolate had a serotype A structure. The second relapse isolate was a mixture of structures composed of serotype D components, glucuronomannan (GM), and a minor serotype A component. Analysis of the initial isolate from patient SB6 showed a structure composed mainly of serotype D, GM, and minor serotype A components and components not assigned to a particular serotype (N). The relapse isolate had the same composition as the initial isolate except for an increase in the serotype A component. This increase in the serotype A component of the relapse isolate resulted in a change in the serological specificity from serotype D to serotype A/D. The initial isolate from patient J9 had serotype D and GM structures. The first two relapse isolates had serotype D, N, and GM structures and a minor serotype A component. The third relapse isolate had mainly a serotype D structure. All the J9 isolates reacted only with serotype D-specific factor serum. These results indicate that some isolates obtained from patients with recurrent C. neoformans infections have undergone a change in GXM structure during the course of infection. The modification of GXM structure observed in some relapse isolates is reflected in changed serological properties. The results may have important implications for the design of vaccines and antibody-based therapeutic strategies against C. neoformans.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Cherniak R,Morris LC,Belay T,Spitzer ED,Casadevall Adoi
10.1128/IAI.63.5.1899-1905.1995subject
Has Abstractpub_date
1995-05-01 00:00:00pages
1899-905issue
5eissn
0019-9567issn
1098-5522journal_volume
63pub_type
杂志文章abstract::To demonstrate local synthesis of anti-Mycobacterium leprae antibodies, biopsies from skin lesions of leprosy patients were cultured in vitro in a medium containing 14C-labeled lysine and isoleucine, and the culture fluids were analyzed by crossed immunoelectrophoresis with intermediate gel and autoradiography. The re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.297-301.1980
更新日期:1980-02-01 00:00:00
abstract::We have developed an in vitro assay to study the intramacrophage replication of Mycobacterium tuberculosis or Mycobacterium bovis BCG. In this assay, extracellular mycobacterial replication was avoided by using a small infecting inoculum and a new method for efficiently removing nonphagocytocytized bacilli. The abilit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.2.786-793.1982
更新日期:1982-08-01 00:00:00
abstract::Shiga-like toxins (SLTs), which are produced by certain strains of Escherichia coli, are composed of enzymatically active A and B subunit multimers responsible for the toxin's binding. We have previously purified large amounts of the SLT-I B subunit by using a hyperexpression vector in Vibrio cholerae under the contro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.1.301-308.1995
更新日期:1995-01-01 00:00:00
abstract::Streptococcus pneumoniae (the pneumococcus), a leading cause of bacterial disease, is most commonly carried in the human nasopharynx. Colonization induces inflammation that promotes the organism's growth and transmission. This inflammatory response is dependent on intracellular sensing of bacterial components that acc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00224-15
更新日期:2015-08-01 00:00:00
abstract::Group A streptococci of M protein type 5 have been epidemiologically related to acute rheumatic fever in a number of reported outbreaks. Preliminary bacteriological evidence suggests that M5 may be an important "rheumatogenic" type in Santiago, Chile. To assess further the relationship of this streptococcal serotype t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.573-579.1982
更新日期:1982-11-01 00:00:00
abstract::The toxicity of Chlamydia trachomatis was studied with mouse peritoneal macrophage culture. Inoculation of 30 inclusion-forming units of trachoma B/TW-5/OT organisms and 250 inclusion-forming units of lymphogranuloma venereum L2/434/Bu organisms per cell caused immediated toxicity, with the killing of 40 to 90% of the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.613-618.1978
更新日期:1978-06-01 00:00:00
abstract::Escherichia coli is a major cause of enteric/diarrheal diseases, urinary tract infections, and sepsis. E. coli K1 is the leading gram-negative organism causing neonatal meningitis, but the microbial basis of E. coli K1 meningitis is incompletely understood. Here we employed comparative genomic hybridization to investi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.4.2196-2206.2006
更新日期:2006-04-01 00:00:00
abstract::One-day-old chicks were infected orally with two strains of weakly hemolytic spirochetes isolated from a human and a pig with intestinal spirochetosis. These spirochetosis both colonized birds, attached end-on to their cecal enterocytes, induced watery diarrhea, and significantly depressed growth rates. Cultures of Se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3705-3710.1995
更新日期:1995-09-01 00:00:00
abstract::Since secondary Streptococcus pneumoniae infections greatly increase the mortality of influenza infections, we determined the relative roles of neutrophil-dependent and -independent mechanisms in increased susceptibility to S. pneumoniae during influenza infection. Mice infected with influenza for 6 days, but not 3 da...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00789-06
更新日期:2006-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1860-1866.1994
更新日期:1994-05-01 00:00:00
abstract::By subcutaneous treatment with an aqueous solution of 6-O-stearoyl-N-acetylmuramyl-L-alanyl-D-isoglutamine [6-O-CH3-(CH2)16-CO-MurNAc-L-Ala-D-isoGln] [referred to here as L18-MDP(Ala)], an augmentation of the resistance of mice to Escherichia coli, Pseudomonas aeruginosa. Staphylococcus aureus, and Candida albicans in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.292-300.1982
更新日期:1982-07-01 00:00:00
abstract::Mouse peritoneal macrophages were infected with varying numbers of Nocardia asteroides 10905, and the fate of the ingested organisms was determined by viable plate count (VPC), light microscopy (LM), immunofluorescent microscopy (IM), and electron microscopy (EM). The results obtained with these methods differed. VPC ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.3.576-590.1974
更新日期:1974-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2571-2576.2003
更新日期:2003-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1114-1116.1984
更新日期:1984-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.2865-2871.2001
更新日期:2001-05-01 00:00:00
abstract::The glycolipid haptens of Mycoplasma pneumoniae became immunogenic when bound to membrane proteins of Acholeplasma laidlawii by reaggregation. This process consisted of the solubilization of lipid-depleted A. laidlawii membranes and M. pneumoniae glycolipids in 20 mm sodium dodecyl sulfate and dialysis of the mixed so...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.3.420-423.1971
更新日期:1971-03-01 00:00:00
abstract::We reexamined the binding specificity of the Shiga-like toxin variant associated with porcine edema disease, SLT2e, which is reported to be more cytotoxic for Vero cells than for HeLa cells, by using receptor-deficient cells and a liposomal insertion system for purified glycolipids. We found that SLT2e preferentially ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.1138-1141.1995
更新日期:1995-03-01 00:00:00
abstract::Rickettsia prowazeki, pretreated with typhus immune human serum, readily infects, and grows in, chicken embryo cells in culture. This finding is similar to those of previous studies which showed that typhus rickettsiae, pretreated with immune serum, grow in cells of the yolk sac of embryonated hen eggs and in the cell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.3.571-575.1974
更新日期:1974-03-01 00:00:00
abstract::An enterotoxin produced by Bacteroides fragilis was purified to homogeneity and characterized as to its biological activity and basic molecular properties. Toxin preparations were prepared by growing B. fragilis VPI 13784 in brain heart infusion broth to early stationary phase, immediately precipitating the culture su...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1343-1350.1992
更新日期:1992-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4326-4333.1999
更新日期:1999-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.8453-8455.2005
更新日期:2005-12-01 00:00:00
abstract::The bacterial pathogen Burkholderia pseudomallei invades host cells, escapes from endocytic vesicles, multiplies intracellularly, and induces the formation of actin tails and membrane protrusions, leading to direct cell-to-cell spreading. This study was aimed at the identification of B. pseudomallei genes responsible ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01262-05
更新日期:2006-06-01 00:00:00
abstract::Intestinal epithelial cells (IECs), including secretory goblet cells, form essential physiochemical barriers that separate luminal bacteria from underlying immune cells in the intestinal mucosa. IECs are common targets for enteric bacterial pathogens, with hosts responding to these microbes through innate toll-like re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02045-14
更新日期:2014-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.976-983.1995
更新日期:1995-03-01 00:00:00
abstract::Placenta-sequestering Plasmodium falciparum involved in the pathogenesis of pregnancy-associated malaria (PAM) in otherwise clinically immune women expresses particular variant surface antigens (VSA(PAM)) on the surface of infected erythrocytes that differ from VSA found in parasitized nonpregnant individuals (non-PAM...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.4112-4118.2005
更新日期:2005-07-01 00:00:00
abstract::The present study was performed to determine whether Histoplasma capsulatum (yeast phase) is able to activate the alternative complement pathway. H. capsulatum yeasts were shown to consume C3 in C4-deficient guinea pig serum. Immunoelectrophoretic conversion of alternative pathway component factor B confirmed that C3 ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.30.1.147-149.1980
更新日期:1980-10-01 00:00:00
abstract::The Fcγ receptor IIIb (FcγRIIIb) is a low-affinity receptor of IgG and is essential in neutrophil-mediated effector functions. Different allelic forms of FcγRIIIb carrying human neutrophil antigen (HNA-1a, -1b, -1c, and -1d) have been identified. Here, we have generated stable transfected HEK293 cell lines expressing ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00924-17
更新日期:2018-07-23 00:00:00
abstract::F1651 and the pyelonephritis-associated pili (Pap) are two members of the type P family of adhesive factors. They play a key role in establishing disease caused by extraintestinal pathogenic Escherichia coli (ExPEC) strains in animals and humans. Both F1651 and Pap are under the control of an epigenetic and reversible...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02510-14
更新日期:2015-05-01 00:00:00
abstract::Wild-type Bordetella bronchiseptica and a bvg mutant strain were used for invasion and survival experiments in human Caco-2 and A549 epithelial cells. Both bacterial strains were able to enter and persist within the host cells for at least a week. A significant proportion of the bacteria from both B. bronchiseptica st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.3008-3011.1994
更新日期:1994-07-01 00:00:00
abstract::Escherichia coli 83972 is a clinical asymptomatia bacteriuric isolate that is able to colonize the human urinary bladder without inducing an immune response. Here we demonstrate that one of the mechanisms by which this strain has become attenuated is through the mutation of its genes encoding type 1 and P fimbriae. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.781-785.2006
更新日期:2006-01-01 00:00:00