Abstract:
:Shiga-like toxins (SLTs), which are produced by certain strains of Escherichia coli, are composed of enzymatically active A and B subunit multimers responsible for the toxin's binding. We have previously purified large amounts of the SLT-I B subunit by using a hyperexpression vector in Vibrio cholerae under the control of the trc promoter. In this study we examined various expression vectors to maximize yields of the SLT-II B subunit. The SLT-II B subunit has been expressed by using both the T7 promoter and the tac promoter in E. coli. When expressed from a plasmid containing the structural gene for SLT-II B deleted of the leader sequence, SLT-II B was able to form multimers when cross-linked, although SLT-II B production from this plasmid was unreproducible. SLT-II B expressed in all three systems appeared to form unstable multimers, which did not readily bind to a monoclonal antibody which preferentially recognizes B subunit multimers. SLT-II B expression was not increased by moving any of the plasmids into V. cholerae. Polyclonal antibodies raised to SLT-II B in rabbits recognized B subunit in SLT-II holotoxin yet were poorly neutralizing. SLT-II B was also expressed as a fusion protein with maltose-binding protein and could be cleaved from maltose-binding protein with factor Xa. Although the expression vectors were able to make large amounts of SLT-II B, as determined by Western blotting (immunoblotting), the levels of purified SLT-II B subunit were low compared with those obtained previously for SLT-I B subunit, probably because of instability of the multimeric SLT-II B subunit.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Acheson DW,De Breucker SA,Jacewicz M,Lincicome LL,Donohue-Rolfe A,Kane AV,Keusch GTdoi
10.1128/IAI.63.1.301-308.1995subject
Has Abstractpub_date
1995-01-01 00:00:00pages
301-8issue
1eissn
0019-9567issn
1098-5522journal_volume
63pub_type
杂志文章abstract::Phagocytosis and killing by alveolar macrophages from humans, rabbits, rats, and hamsters, were compared in vitro. In the absence of serum opsonins, human alveolar macrophages could phagocytize Staphylococcus aureus Cowan I (protein A positive), but not S. aureus EMS (protein A negative) or Pseudomonas aeruginosa MN. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.2.504-509.1982
更新日期:1982-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01886-06
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journal_title:Infection and immunity
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doi:10.1128/IAI.72.9.5483-5486.2004
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.3780-3789.1995
更新日期:1995-10-01 00:00:00
abstract::Four monoclonal antibodies against Escherichia coli J5 were studied. Each of these monoclonal antibodies reacted with purified lipopolysaccharides from E. coli J5, the deep rough mutant Salmonella minnesota Re595, Agrobacterium tumefaciens, and Pseudomonas aeruginosa PAO1 as well as with the purified lipid A of P. aer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.631-636.1984
更新日期:1984-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.5301-5308.1997
更新日期:1997-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.4.679-682.1973
更新日期:1973-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00080-06
更新日期:2006-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.6.3524-3530.2004
更新日期:2004-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/iai.68.3.1289-1296.2000
更新日期:2000-03-01 00:00:00
abstract::Infection with Cryptosporidium parvum, a ubiquitous protozoan parasite of virtually all mammals, can cause mild to severe diarrhea in immunocompetent hosts and life-threatening diarrhea in immunocompromised hosts. Passive immunotherapy of experimentally infected animals and naturally infected humans with hyperimmune b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.2962-2965.1990
更新日期:1990-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.2.392-400.1992
更新日期:1992-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01328-06
更新日期:2007-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00441-13
更新日期:2013-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.794-799.2001
更新日期:2001-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.2.317-323.1986
更新日期:1986-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.554-559.1981
更新日期:1981-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1314-1321.1992
更新日期:1992-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00036-09
更新日期:2009-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.5.815-820.1974
更新日期:1974-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.785-790.1989
更新日期:1989-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2001-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3385-3393.1996
更新日期:1996-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5161-5165.1996
更新日期:1996-12-01 00:00:00
abstract::The effector mechanism, which operates against challenge parasites in the lungs of C57BL/6 mice vaccinated once with irradiated cercariae of Schistosoma mansoni, is mediated by CD4+ T helper lymphocytes. However, adoptive transfer of immunity from vaccinated donors to naive recipients by using sensitized T cells has n...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.1.42-48.1997
更新日期:1997-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.4.1103-1109.1976
更新日期:1976-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Synthetic Escherichia coli lipid A and synthetic S-[2,3-bis-(palmitoyloxy)propyl]-N-palmitoylpentapeptide (tripalmitoyl pentapeptide [TPP]), representing the mitogenically active principles of bacterial lipopolysaccharide (LPS) and lipoprotein, respectively, were compared for their mitogenic activities on splenocytes ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1382-1384.1988
更新日期:1988-05-01 00:00:00
abstract::Bacteria exhibit extensive genetic heterogeneity within species. In many cases, these differences account for virulence properties unique to specific strains. Several such loci have been discovered in the genome of the type b serotype of Haemophilus influenzae, a human pathogen able to cause meningitis, pneumonia, and...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2003-03-01 00:00:00