Abstract:
:Phagocytosis and killing by alveolar macrophages from humans, rabbits, rats, and hamsters, were compared in vitro. In the absence of serum opsonins, human alveolar macrophages could phagocytize Staphylococcus aureus Cowan I (protein A positive), but not S. aureus EMS (protein A negative) or Pseudomonas aeruginosa MN. In contrast, rabbit, rat, and hamster alveolar macrophages did not phagocytize S. aureus Cowan I or other nonopsonized bacteria. Human alveolar macrophages, but not other species, stained positively with fluorescein isothiocyanate-conjugated protein A. When opsonized bacterial were studied, phagocytosis by human, rabbit, and hamster alveolar macrophages was found to be mediated by both Fc and C3 receptors. However, only Fc receptor-mediated phagocytosis of bacteria was demonstrated for rat alveolar macrophages. Differences were also found in the kinetics of bacterial killing by alveolar macrophages from different species. Human and rabbit alveolar macrophages rapidly killed opsonized S. aureus Cowan I. However, bacterial killing by hamster alveolar macrophages proceeded at a slower rate, and rat alveolar macrophages completely failed to kill S. aureus. These significant differences in the function of alveolar macrophages from four different species emphasize the need to document the appropriateness of animal models before using them to predict the biological activities of human alveolar macrophages.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Nguyen BY,Peterson PK,Verbrugh HA,Quie PG,Hoidal JRdoi
10.1128/IAI.36.2.504-509.1982subject
Has Abstractpub_date
1982-05-01 00:00:00pages
504-9issue
2eissn
0019-9567issn
1098-5522journal_volume
36pub_type
杂志文章abstract::A peptidoglycan layer of Treponema pallidum kazan was isolated by solubilization of whole cells with 1% warm sodium dodecyl sulfate and subsequent digestion of an insoluble residue with proteases. Electron microscopy revealed that the peptidoglycan was isolated as a single-layered sacculus of less than 5 nm in thickne...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.767-774.1981
更新日期:1981-02-01 00:00:00
abstract::Decay-accelerating factor (DAF), a complement regulatory protein, also serves as a receptor for Dr adhesin-bearing Escherichia coli. The repeat three of DAF was shown to be important in Dr adhesin binding and complement regulation. However, Dr adhesins do not bind to red blood cells with the rare polymorphism of DAF, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4485-4493.2002
更新日期:2002-08-01 00:00:00
abstract::Various features of salmonellosis were examined in a burned-mouse model. In this model, which uses an outbred mouse strain, a challenge dose of ca. 100 CFU with any of several strains of Salmonella typhimurium caused a fatal infection. A variety of mutated strains attenuated for virulence in Salmonella-susceptible par...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.9.2842-2846.1989
更新日期:1989-09-01 00:00:00
abstract::In vitro, Clostridium perfringens enterotoxin (CPE) binds to human ileal epithelium and induces morphological damage concurrently with reduced short-circuit current, transepithelial resistance, and net water absorption. CPE also binds to the human colon in vitro but causes only slight morphological and transport chang...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.8407-8410.2005
更新日期:2005-12-01 00:00:00
abstract::A parasitic protozoan, Leishmania mexicana amazonensis, was previously made resistant to tunicamycin (J.A. Kink and K.-P. Chang, Proc. Natl. Acad. Sci. USA 84:1253-1257, 1987). In the present study, six different tunicamycin-resistant variants were biologically and biochemically compared with their parental wild type ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.7.1692-1700.1987
更新日期:1987-07-01 00:00:00
abstract::We examined the secretion of antimicrobial proteins and peptides into surgically isolated and continuously perfused segments of rat small intestine. Up to nine discrete antimicrobial molecules appeared in the intestinal perfusates following intravenous administration of bethanechol, a cholinergic agonist, or intralume...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5161-5165.1996
更新日期:1996-12-01 00:00:00
abstract::Most Escherichia coli isolates can express type 1 fimbriae with mannose-specific adhesins. These adhesins bind to the oligosaccharide chains of secretory immunoglobulin A (IgA). Thus, in addition to specific antibody activity, secretory IgA possesses a broad reactivity with bacteria expressing type 1 fimbriae. The abs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2794-2798.1996
更新日期:1996-07-01 00:00:00
abstract::Virulent Streptococcus suis serotype 2 strains are invasive extracellular bacteria causing septicemia and meningitis in piglets and humans. One objective of this study was to elucidate the function of complement in innate immune defense against S. suis. Experimental infection of wild-type (WT) and C3(-/-) mice demonst...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00080-14
更新日期:2014-06-01 00:00:00
abstract::Subtilase cytotoxin (SubAB) is a recently identified AB5 subunit toxin produced by Shiga-toxigenic Escherichia coli. The A subunit is thought to be a subtilase-like, serine protease, whereas the B subunit binds to the toxin receptor on the cell surface. We cloned the genes from a clinical isolate; the toxin was produc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01336-06
更新日期:2007-01-01 00:00:00
abstract::The major antigenic determinants of the lipoglycans from Acholeplasma granularum and Acholeplasma axanthum were found to be the oligosaccharide sequences Glcp(beta 1 leads to 2)-Glcp(alpha 1 leads to 4)-Glcp and Glcp(beta 1 leads to 2)-Glcp(beta 1 leads to 2)-Glcp, respectively. The disaccharides sophorose and maltose...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.629-632.1983
更新日期:1983-05-01 00:00:00
abstract::Peru-15 is a live attenuated oral vaccine derived from a Vibrio cholerae O1 El Tor Inaba strain by a series of deletions and modifications, including deletion of the entire CT genetic element. Peru-15 is also a stable, motility-defective strain and is unable to recombine with homologous DNA. We wished to determine whe...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/iai.70.4.1965-1970.2002
更新日期:2002-04-01 00:00:00
abstract::Infection of epithelial cells by two biovars of Chlamydia trachomatis results in the tyrosine phosphorylation of several host proteins. The most prominent change in host protein tyrosine phosphorylation involves a complex of proteins with molecular masses of 75 to 85 kDa (pp75/85) and 100 kDa (pp100). The C. trachomat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.5301-5308.1997
更新日期:1997-12-01 00:00:00
abstract::Serial passage of Cryptococcus neoformans in mice increases virulence relative to the nonpassaged line. Postpassaged lines showed no difference in the expression of most known virulence factors, with the exception that the more virulent lines had smaller capsules in vitro. These data imply that other mechanisms of vir...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.7047-7050.2005
更新日期:2005-10-01 00:00:00
abstract::We have shown that a member of the 70-kDa heat shock protein (Hsp70) family is a major target of the humoral immune response during Leishmania donovani infection. A recombinant fusion protein was recognized by sera from 92% (35 of 38) of patients with visceral leishmaniasis, including representatives from each of the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2688-2693.1992
更新日期:1992-07-01 00:00:00
abstract::Coagulation and inflammation are interconnected, suggesting that coagulation plays a key role in the inflammatory response to pathogens. A phenome-wide association study (PheWAS) was used to identify clinical phenotypes of patients with a polymorphism in coagulation factor X. Patients with this single nucleotide polym...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00031-19
更新日期:2019-04-23 00:00:00
abstract::Recent studies have shown that probiotics are beneficial in T-cell-mediated inflammatory diseases. The molecular mechanism by which probiotics work remains elusive, but accumulating evidence indicates that probiotics can modulate immune cell responses. Since T cells express receptors for bacterial products or componen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01172-06
更新日期:2007-04-01 00:00:00
abstract::The relationship between the sequence of Neisseria gonorrhoeae pilin and its quaternary assembly into pilus fibers was studied with a set of site-directed antibody probes and by mapping the specificities of antipilus antisera with peptides. Buried and exposed peptides in assembled pili were identified by competitive i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.2.644-652.1996
更新日期:1996-02-01 00:00:00
abstract::Streptococcus pneumoniae contains an inhibitor of human neutrophil elastase. The agent does not inhibit other proteases, including neutrophil cathepsin G and pancreatic elastase. It is active in the presence of insoluble elastin as well as synthetic elastase substrates. The inhibitor is present in the pneumococcal cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.1.52-60.1985
更新日期:1985-07-01 00:00:00
abstract::Preexisting antivector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. To circumvent this problem, we injected CpG DNA ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00700-09
更新日期:2009-12-01 00:00:00
abstract::Pseudomonas aeruginosa elastase rapidly inactivates alpha 1-proteinase inhibitor by splitting its Pro-357-Met-358 peptide bond. The present study was aimed at testing whether this reaction takes place in the presence of leukocyte elastase. To this end was added alpha 1-proteinase inhibitor to a mixture of the two elas...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.12.3793-3797.1989
更新日期:1989-12-01 00:00:00
abstract::Peptidoglycan (PG) turnover in exponentially growing Neisseria gonorrhoeae RD5 type 4 was accompanied by release of soluble PG fragments into the medium. Turnover of the D-[14C]glucosamine-labeled glycan moiety and of the meso-[3H]diaminopimelic acid (DAP)-labeled peptide region occurred at similar rates (ca. 35% per ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.869-878.1979
更新日期:1979-06-01 00:00:00
abstract::The response of the intracellular pathogen Rhodococcus equi to H(2)O(2) treatment, a situation potentially encountered after the oxidative burst of alveolar macrophages, was analyzed. Compared to other bacteria, including Deinococcus radiodurans, R. equi showed exceptionally high resistance to this stress. A proteomic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3768-3776.2002
更新日期:2002-07-01 00:00:00
abstract::Isolated outer membranes of Borrelia burgdorferi 297 were utilized to obtain partial amino acid sequence information for a low-molecular-weight, outer membrane-associated polypeptide. Degenerate oligonucleotide primers based upon this information were used to amplify a 100-bp probe for detection of the corresponding f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.412-421.1997
更新日期:1997-02-01 00:00:00
abstract::One of the first steps in the development of cerebral toxoplasmosis is the penetration of the blood-brain barrier, which is comprised of microvascular endothelial cells. We examined the capacity of human brain microvascular endothelial cells (HBMEC) to interact with Toxoplasma gondii. We found that stimulation of HBME...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.10.6527-6531.2001
更新日期:2001-10-01 00:00:00
abstract::Black melanin-like pigments are produced by several neurotropic fungi, including Cryptococcus neoformans. Pigment production is associated with virulence. In media containing phenolic substrates such as L-dopa, C. neoformans cells become black as a result of pigment accumulation. Pigmented and nonpigmented C. neoforma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.8.3131-3136.1995
更新日期:1995-08-01 00:00:00
abstract::Immunization with live Plasmodium sporozoites under chloroquine prophylaxis (Spz plus CQ) induces sterile immunity against sporozoite challenge in rodents and, more importantly, in humans. Full protection is obtained with substantially fewer parasites than with the classic immunization with radiation-attenuated sporoz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01415-09
更新日期:2010-05-01 00:00:00
abstract::Exoenzyme Y (ExoY) is a type III secretion system effector found in 90% of the Pseudomonas aeruginosa isolates. Although it is known that ExoY is a soluble nucleotidyl cyclase that increases the cytoplasmic levels of nucleoside 3',5'-cyclic monophosphates (cNMPs) to mediate endothelial Tau phosphorylation and permeabi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00239-17
更新日期:2017-08-18 00:00:00
abstract::Isolated Bacteroides gingivalis 2561 vesicles aggregated suspensions of Actinomyces viscosus and Actinomyces naeslundii of all taxonomy clusters. Vesicles bound near A. viscosus cell walls and among its surface fibrils. Tritiated vesicles bound slightly better to saliva-coated hydroxyapatite (SHA) than to SHA coated w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1618-1620.1989
更新日期:1989-05-01 00:00:00
abstract::Only 20 to 40% of human blood monocytes were capable of responding to chemotaxins in vitro. This limit is not due to restrictions of the in vitro system, but is due to the existence of a migrating subpopulation. Over a wide range, the number of cells migrating toward a given concentration of chemotaxin was directly pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00
abstract::Brucella abortus is a facultative intracellular bacterial pathogen that causes abortion in domestic animals and undulant fever in humans. The mechanism of virulence of Brucella spp. is not fully understood yet. Furthermore, genes that allow Brucella to reach the intracellular niche and to interact with host cells need...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.9.5036-5044.2002
更新日期:2002-09-01 00:00:00