Abstract:
:We reexamined the binding specificity of the Shiga-like toxin variant associated with porcine edema disease, SLT2e, which is reported to be more cytotoxic for Vero cells than for HeLa cells, by using receptor-deficient cells and a liposomal insertion system for purified glycolipids. We found that SLT2e preferentially uses globotetraosylceramide as a receptor but can also cause cytotoxicity by using globotriaosylceramide, the SLT2 receptor. We conclude that the differential cytotoxicity of SLT2e on HeLa and Vero cells is a function of both the receptor preference of the toxin and the specific glycolipid content of the target cells being used.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Keusch GT,Jacewicz M,Acheson DW,Donohue-Rolfe A,Kane AV,McCluer RHdoi
10.1128/IAI.63.3.1138-1141.1995subject
Has Abstractpub_date
1995-03-01 00:00:00pages
1138-41issue
3eissn
0019-9567issn
1098-5522journal_volume
63pub_type
杂志文章abstract::Antigen-presenting cells (APCs) are key players in the induction and regulation of immune responses. In Plasmodium falciparum malaria, determination of which cells and pathways are activated in the network of APCs remains elusive. We therefore investigated the effects of a controlled human malaria infection in healthy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00473-15
更新日期:2015-09-01 00:00:00
abstract::The membrane-damaging properties on human diploid embryonic lung fibroblasts of streptolysin O (from Streptococcus pyogenes) and theta-toxin (from Clostridium perfringens) were compared. The results are consistent with the suggested mechanism for hemolysis by streptolysin O involving one fixation site and one lytic si...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00
abstract::Host-adapted Gram-negative bacterial pathogens from the Pasteurellaceae, Neisseriaceae, and Moraxellaceae families normally reside in the upper respiratory or genitourinary tracts of their hosts and rely on utilizing iron from host transferrin (Tf) for growth and survival. The surface receptor proteins that mediate th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02572-14
更新日期:2015-03-01 00:00:00
abstract::It was found that cells of Nocardia asteroides GUH-2 (virulent) were approximately 10 times more virulent than cells of N. asteroides 14759 (intermediate) and greater that 500 times more virulent than N. asteroides 10905 (avirulent) cells when early-stationary-phase cultures suspended in saline were injected intraven...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.3.995-1004.1977
更新日期:1977-06-01 00:00:00
abstract::A protein factor which induces high levels of gamma interferon (IFN-gamma) in resting splenic nonadherent cells was isolated from the sera of mice with generalized inflammation caused by endotoxic shock. The factor was highly purified by ammonium sulfate precipitation followed by ion-exchange column chromatography on ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.64-70.1993
更新日期:1993-01-01 00:00:00
abstract::The lymphogranuloma venereum (LGV) and trachoma biovars of Chlamydia trachomatis exhibit differences in biological properties both in vivo and in vitro. To identify analogous biochemical differences, we studied the molecular charges of chlamydial outer membrane proteins (OMPs) by means of isoelectric focusing and none...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.488-494.1985
更新日期:1985-11-01 00:00:00
abstract::The bactericidal activity of guinea pig and human polymorphonuclear leukocytes (PMNs) against a smooth-intermediate strain (45/0) and a rough strain (45/20) of Brucella abortus has been examined. After incubation for 120 min, guinea pig PMNs incubated with either the smooth strain 45/0 or the rough strain 45/20 exhibi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.3.737-742.1979
更新日期:1979-03-01 00:00:00
abstract::The role of CD8 T cells in controlling Mycobacterium tuberculosis infections in mice was confirmed by comparing the levels of growth of the organism in control, major histocompatibility complex class II knockout, and athymic mice and by transferring T-cell populations into athymic mice. By using donor mice which were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.830-834.1998
更新日期:1998-02-01 00:00:00
abstract::Normal bacteria can translocate (migrate) across an intact intestinal mucosa, but the anatomical site of bacterial translocation has not been defined. Gastrointestinal (GI) cross-sections were obtained from mice monoassociated with high cecal concentrations of Escherichia coli, Proteus mirabilis, or Enterococcus faeca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.12.4693-4697.1991
更新日期:1991-12-01 00:00:00
abstract::A solid-phase radioimmunoassay, applying whole Mycobacterium leprae as antigen and radiolabeled protein A from Staphylococcus aureus as antibody-detecting reagent, was used for the determination of specific immunoglobulin G (IgG) and IgM antibody responses in leprosy patients. High IgG anti-M. leprae antibody levels w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.3.885-892.1982
更新日期:1982-06-01 00:00:00
abstract::The recent finding that high numbers of strict anaerobes are present in the respiratory tract of cystic fibrosis (CF) patients has drawn attention to the pathogenic contribution of the CF microbiome to airway disease. In this study, we investigated the specific interactions of the most dominant bacterial CF pathogen, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02234-14
更新日期:2015-01-01 00:00:00
abstract::gammadelta T cells are considered crucial to the outcome of various infectious diseases. The present study was undertaken to characterize gammadelta (T-cell receptor 1(+) [TCR1(+)]) T cells phenotypically and functionally in avian immune response. Day-old chicks were orally immunized with Salmonella enterica serovar E...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01128-05
更新日期:2006-07-01 00:00:00
abstract::Previous studies in our laboratory have indicated that naturally resistant, inbred DBA/2J mice mount a greater serum antibody response to Pseudomonas aeruginosa 19660 than susceptible C57BL/6J mice. However, the specificity of the antibody produced was not known. The present study examines the specificity and kinetics...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.1984-1990.1991
更新日期:1991-06-01 00:00:00
abstract::A soluble hemagglutinin/protease produced by Vibrio cholerae, which has previously been shown to hydrolyze fibronectin and ovomucin and to cleave lactoferrin and the A subunit of the heat-labile enterotoxin of Escherichia coli, appears to be a zinc metalloendopeptidase. Both its hemagglutinative and protease functions...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.2.639-644.1983
更新日期:1983-11-01 00:00:00
abstract::We wished to find a simple, biologically relevant method to evaluate the virulence of dengue viruses for human beings. Since cells of mononuclear phagocyte lineage may be important sites of dengue infection in primates, we evaluated the permissiveness of these cells to dengue virus as a correlate of virus virulence. T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.102-106.1981
更新日期:1981-01-01 00:00:00
abstract::Surface proteins in gram-positive bacteria are frequently required for virulence, and many are attached to the cell wall by sortase enzymes. Bacteria frequently encode more than one sortase enzyme and an even larger number of potential sortase substrates that possess an LPXTG-type cell wall sorting signal. In order to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.2710-2722.2004
更新日期:2004-05-01 00:00:00
abstract::The character of the antibody response in the rabbit to Salmonella typhimurium somatic (O) antigen was similar to the response to each of several serotypes of Shigella flexneri O antigens, namely a predominance of production of immunoglobulin M (IgM) antibody. Lipopolysaccharide protein (LPSP) and lipopolysaccharide (...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1970-03-01 00:00:00
abstract::The yeast form of the dimorphic fungus Histoplasma capsulatum survives within macrophages after phagocytosis. To do so, it must avoid, inhibit, or resist a variety of toxic oxygen metabolites. Using ferricytochrome c reduction to assay superoxide release, we examined the response of mouse macrophages to the yeast form...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.1.29-34.1987
更新日期:1987-01-01 00:00:00
abstract::Peptidoglycans of Leptotrichia buccalis ATCC 14201 and ATCC 19616 were isolated by extraction with sodium dodecyl sulfate and subsequent digestion of the sodium dodecyl sulfate-insoluble residue with proteases and alpha-amylase. Cell wall fractions obtained by sodium dodecyl sulfate extraction and protease digestion w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.1.132-136.1983
更新日期:1983-01-01 00:00:00
abstract::The generation of leukotrienes (LTC4, LTD4, LTE4, and LTB4; 12-epi-LTB4 isomer) from human granulocytes by thiol-activated toxins (streptolysin O, alveolysin from Bacillus alvei, and theta toxin from Clostridium perfringens) is described. The release occurs under noncytolytic conditions. Although LTB4 is the major com...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.3.844-851.1985
更新日期:1985-12-01 00:00:00
abstract::The mechanism of enhanced resistance of Mycobacterium bovis BCG-treated mice to ectromelia virus infection was investigated by determining the effect of splenectomy, antithymocyte serum, and antimacrophage serum on resistance. It was greatly reduced by these treatments, not only in normal mice, but also in mice treate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.2.567-573.1983
更新日期:1983-11-01 00:00:00
abstract::Adherence to fibrinogen and fibronectin plays a crucial role in Staphylococcus aureus experimental endocarditis. Previous genetic studies have shown that infection and carriage isolates do not systematically differ in their virulence-related genes, including genes conferring adherence, such as clfA and fnbA. We set ou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01274-09
更新日期:2010-04-01 00:00:00
abstract::The zoopathogenic fungus Histoplasma capsulatum requires iron for growth. Intracellular growth of the fungus within mouse peritoneal macrophages is inhibited by recombinant murine gamma interferon (IFN-gamma). Such treatment of mouse peritoneal macrophages induces a marked downshift in transferrin receptors. We tested...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.7.2274-2278.1991
更新日期:1991-07-01 00:00:00
abstract::The immune mechanism by which hamsters acquire resistance to infection with Treponema pertenue, the causative agent of frambesia, or yaws, has not been elucidated. Serum or cells (spleen or lymph node) obtained from hamsters resistant to frambesial infection were transferred to normal syngenic recipients, who are subs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.430-435.1978
更新日期:1978-08-01 00:00:00
abstract::Hematogenous infections caused by Candida krusei have been noted with increasing frequency, particularly in cancer patients receiving prophylaxis with antifungal triazoles. Progress in understanding the pathogenesis of this emerging infection has been limited by the lack of an animal model. We developed a CF1 mouse in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.4.1268-1271.1993
更新日期:1993-04-01 00:00:00
abstract::Previous passive antibody transfer experiments have indicated that immunity to a 46-kilodalton membrane glycoprotein (M-2) of Leishmania amazonensis may protect against infection with this parasite. In the studies described in this paper, we investigated the ability of the purified M-2 molecule to elicit a protective ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3272-3279.1988
更新日期:1988-12-01 00:00:00
abstract::The immunopathology of Chagas' disease was studied in the experimental model of chronic infection in C57BL/10JT or mice. Sublethal infection with Trypanosoma cruzi, Y strain, induced specific antibodies and a delayed hypersensitivity response to parasite antigens. Mice developed chronic chagasic myocarditis but not sk...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.11.3588-3593.1990
更新日期:1990-11-01 00:00:00
abstract::The Haemophilus ducreyi 35000HP genome encodes a homolog of the CpxRA two-component cell envelope stress response system originally characterized in Escherichia coli. CpxR, the cytoplasmic response regulator, was shown previously to be involved in repression of the expression of the lspB-lspA2 operon (M. Labandeira-Re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00678-10
更新日期:2010-11-01 00:00:00
abstract::An immunoregulatory mechanism involving release of neutralizing autoantibodies (Aabs) to self cytokines during bacterial infections is presented herein. Intraperitoneal inoculation of Haemophilus influenzae type b into Sprague-Dawley rats resulted in a self-limiting meningitis. High levels of cells expressing mRNA for...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.8.3300-3303.1997
更新日期:1997-08-01 00:00:00
abstract::The facultative intracellular bacterial pathogen Listeria monocytogenes induces severe fetal infection during pregnancy. Little is known about the molecular mechanisms allowing the maternofetal transmission of bacteria. In this work, we studied fetoplacental invasion by infecting mice with various mutants lacking viru...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01570-06
更新日期:2007-02-01 00:00:00