Abstract:
:The recent finding that high numbers of strict anaerobes are present in the respiratory tract of cystic fibrosis (CF) patients has drawn attention to the pathogenic contribution of the CF microbiome to airway disease. In this study, we investigated the specific interactions of the most dominant bacterial CF pathogen, Pseudomonas aeruginosa, with the anaerobic bacterium Veillonella parvula, which has been recovered at comparable cell numbers from the respiratory tract of CF patients. In addition to growth competition experiments, transcriptional profiling, and analyses of biofilm formation by in vitro studies, we used our recently established in vivo murine tumor model to investigate mutual influences of the two pathogens during a biofilm-associated infection process. We found that P. aeruginosa and V. parvula colonized distinct niches within the tumor. Interestingly, significantly higher cell numbers of P. aeruginosa could be recovered from the tumor tissue when mice were coinfected with both bacterial species than when mice were monoinfected with P. aeruginosa. Concordantly, the results of in vivo transcriptional profiling implied that the presence of V. parvula supports P. aeruginosa growth at the site of infection in the host, and the higher P. aeruginosa load correlated with clinical deterioration of the host. Although many challenges must be overcome to dissect the specific interactions of coinfecting bacteria during an infection process, our findings exemplarily demonstrate that the complex interrelations between coinfecting microorganisms and the immune responses determine clinical outcome to a much greater extent than previously anticipated.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Pustelny C,Komor U,Pawar V,Lorenz A,Bielecka A,Moter A,Gocht B,Eckweiler D,Müsken M,Grothe C,Lünsdorf H,Weiss S,Häussler Sdoi
10.1128/IAI.02234-14subject
Has Abstractpub_date
2015-01-01 00:00:00pages
417-29issue
1eissn
0019-9567issn
1098-5522pii
IAI.02234-14journal_volume
83pub_type
杂志文章abstract::Immunity to pneumococcal colonization in mice by exposure to live or killed pneumococci has been shown to be antibody independent but dependent on CD4+ T cells. Here we show that intranasal immunization with pneumococcal proteins (pneumococcal surface protein C, adhesin A, and a pneumolysoid) can elicit a similar mech...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00773-07
更新日期:2007-11-01 00:00:00
abstract::The effect of continuous exposure to 80% oxygen on newborn mice with Ureaplasma urealyticum pneumonia was determined. Mice were inoculated intranasally with either U. urealyticum or sterile broth and then housed in either 80% oxygen or room air (21% oxygen). The mice were sacrificed at either 7 or 14 days after inocul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.11.3487-3493.1990
更新日期:1990-11-01 00:00:00
abstract::Human genital Chlamydia infection is a major public health concern due to the serious reproductive system complications. Chlamydia binds several receptor tyrosine kinases (RTKs) on host cells, including the epidermal growth factor receptor (EGFR), and activates cellular signaling cascades for host invasion, cytoskelet...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00819-19
更新日期:2020-03-23 00:00:00
abstract::Although periodontal treatment by scaling and root planing (SCRP) is known to induce bacteremia, the effect of this procedure on the host immune response is not known. We have determined pre- and post-SCRP immunoglobulin G antibody titers to antigens of Actinobacillus actinomycetemcomitans in the sera of 22 patients w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.1.145-151.1994
更新日期:1994-01-01 00:00:00
abstract::The immunoprotective activity of Klebsiella pneumoniae K2 cell surface preparations and purified capsular polysaccharide was tested in mice. The 50% protective dose (PD50), expressed as capsular polysaccharide content, was 2 ng for cell surface preparations and 50 ng for purified capsular polysaccharide. Both preparat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.365-370.1986
更新日期:1986-11-01 00:00:00
abstract::Pasteurella (Mannheimia) haemolytica leukotoxin (Lkt) causes cell type- and species-specific effects in ruminant leukocytes. Recent studies indicate that P. haemolytica Lkt binds to bovine CD18, the common subunit of all beta2 integrins. We designed experiments with the following objectives: to identify which member o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.1.72-79.2000
更新日期:2000-01-01 00:00:00
abstract::Specific stimulation of T cells by phytohemagglutinin and Lens culinaris lectin was inhibited by a soluble factor(s) secreted by normal adherent cells stimulated with culture filtrate protein extract (CFPE) derived from bacterial cultures of Mycobacterium tuberculosis H37Ra (avirulent) and H37Rv (virulent). The induct...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.309-313.1986
更新日期:1986-04-01 00:00:00
abstract::A murine monoclonal antibody was raised against the B95-8 strain of Epstein-Barr virus (EBV), which was isolated from a case of mononucleosis after blood transfusion (Hoffman et al. Proc. Natl. Acad. Sci. U.S.A. 77:2979, 1980). We provide evidence that neutralization of immortalization by this monoclonal antibody is v...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.1028-1031.1982
更新日期:1982-09-01 00:00:00
abstract::In previous work, we identified xanthine oxidase (XO) as an important enzyme in the interaction between the host and enteropathogenic Escherichia coli(EPEC) and Shiga-toxigenic E. coli(STEC). Many of the biological effects of XO were due to the hydrogen peroxide produced by the enzyme. We wondered, however, if uric ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01389-15
更新日期:2016-03-24 00:00:00
abstract::Naegleria fowleri produces fatal meningoencephalitis in humans and in experimentally infected laboratory animals. The course of the disease in mice is dependent upon the infecting dose of amoebae, route of inoculation, and prior exposure to Naegleria antigens. DUB/ICR mice were immunized by various routes and antigen ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.3.817-820.1977
更新日期:1977-06-01 00:00:00
abstract::HbhA of Mycobacterium tuberculosis is a multifunctional binding protein, binding to both sulfated sugars such as heparin and to human complement component C3. HbhA may therefore interact with host molecules and/or host cells during M. tuberculosis infection and play a role in the pathogenesis of this bacterium. The pu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.6751-6760.2002
更新日期:2002-12-01 00:00:00
abstract::Salmonella enterica serovar Pullorum is worldwide a poultry pathogen of considerable economic importance, particularly in those countries with a developing poultry industry. In addition to the characteristic high mortality rates among young chicks, one of the features of Salmonella serovar Pullorum infection is that i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7873-7879.2001
更新日期:2001-12-01 00:00:00
abstract::The 23-membered Esx protein family is involved in the host-pathogen interactions of Mycobacterium tuberculosis. These secreted proteins are among the most immunodominant antigens recognized by the human immune system and have thus been used to develop vaccines and immunodiagnostic tests for tuberculosis (TB). Gene pai...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05344-11
更新日期:2011-10-01 00:00:00
abstract::The enterotoxin of Shigella dysenteriae I produces fluid and electrolyte secretion in the rabbit ileum. These present studies were designed to evaluate nonelectrolyte transport in rabbit ileal mucosa exposed to Shigella enterotoxin. Both 10 mM galactose and 5 mM L-alanine absorptions were significantly impaired in ent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.510-512.1977
更新日期:1977-05-01 00:00:00
abstract::Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALB/c mouse model, while isogenic mutants with mini-Tn5 insertions in pheA, trpB, and dagA display little or no attenuation in cultured murine macrophages or mice. These experimental findings conf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4911-4917.2004
更新日期:2004-08-01 00:00:00
abstract::We have isolated plasma cell hybridomas which secrete monoclonal antibodies directed against Haemophilus ducreyi. Two of these monoclonal antibodies recognize all strains of H. ducreyi tested to date and are capable of detecting the presence of H. ducreyi in skin lesions produced by this pathogen in experimental anima...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.1.196-198.1984
更新日期:1984-04-01 00:00:00
abstract::Specific pathogen-free guinea pigs were infected via the respiratory route with viable, attenuated Mycobacterium tuberculosis H37Ra and maintained on purified isocaloric diets. The control diet contained 30% protein (ovalbumin) and 50 ppm of added zinc (50 micrograms/g), the low protein diet contained 10% protein and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.793-799.1983
更新日期:1983-02-01 00:00:00
abstract::Nonopsonic invasion of mononuclear phagocytes by Mycobacterium tuberculosis is likely important in the establishment of a primary infection in the lung. M. tuberculosis binds to a variety of phagocyte receptors, of which the mannose receptor and complement receptor type 3 (CR3) may support nonopsonic binding. CR3, a b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5373-5383.1996
更新日期:1996-12-01 00:00:00
abstract::Clostridium perfringens enterotoxin is the major virulence factor involved in the pathogenesis of C. perfringens type A food poisoning and several non-food-borne human gastrointestinal illnesses. The enterotoxin gene, cpe, is located on the chromosome of food-poisoning isolates but is found on a large plasmid in non-f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3483-3487.2001
更新日期:2001-05-01 00:00:00
abstract::Fimbrial adhesins that mediate attachment to host cells are produced by most virulent Escherichia coli isolates. These virulence factors play an important role in the initial stages of bacterial colonization and also in determination of the host and tissue specificity. Isolates belonging to serotype O78 are known to c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.10.5901-5907.2000
更新日期:2000-10-01 00:00:00
abstract::Two weeks after mice had been infected in the footpad with cells of Nocardia brasiliensis, immunoglobulin and C3 deposits were observed in tissue from the inoculation site. Both immunoglobulin and C3 were found in the actinomycotic granules that characterize the mycetoma and in the surrounding inflammatory zones, in w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.3.1218-1222.1983
更新日期:1983-06-01 00:00:00
abstract::Survival of macrophage microbicidal activity is a prerequisite for invasive disease caused by the enteric pathogen Salmonella enterica serovar Typhimurium. Flavohemoglobins, such as those of Escherichia coli, Salmonella, and yeast, play vital roles in protection of these microorganisms in vitro from nitric oxide (NO) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4399-4405.2002
更新日期:2002-08-01 00:00:00
abstract::White-footed mice (Peromyscus leucopus), the primary reservoir for Borrelia burgdorferi in the northern midwest and northeastern United States, were experimentally inoculated with an infectious strain or a noninfectious strain of the Lyme disease spirochete and examined for their specific antibody response with the en...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.11.3445-3451.1989
更新日期:1989-11-01 00:00:00
abstract::The importance of CD4+ cells and gamma interferon (IFN-gamma) in the resolution of established Cryptosporidium parvum infection was investigated with a murine model of cryptosporidiosis in severe combined immunodeficient (SCID) mice. C. parvum-infected SCID mice were reconstituted with spleen cells from immunocompeten...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3928-3932.1993
更新日期:1993-09-01 00:00:00
abstract::In this study we investigated the mucosal and systemic responses to two T-cell-independent polysaccharides, a serogroup f polysaccharide (formed of rhamnose glucose polymers [RGPs]) from Streptococcus mutans OMZ 175 and a mannan from Saccharomyces cerevisiae, covalently conjugated either to a linear peptide (peptide 3...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2645-2651.1995
更新日期:1995-07-01 00:00:00
abstract::A defect in lymphocyte function could be responsible for persistent infection by the equine infectious anemia virus. The number of lymphocytes bearing surface immunoglobulin, as detected by immunofluorescence, and lymphocyte response to mitogens were the same in uninfected and equine infectious anemia-infected animals...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.4.679-682.1973
更新日期:1973-10-01 00:00:00
abstract::The lack of specific T-cell help in immune responses to thymus-independent antigens results in weak, predominantly immunoglobulin M-mediated immunity with little or no memory. In the work presented here we show how the exogenous stimulation of CD40 by monoclonal antibodies can mimic T-cell help, resulting in enhanced ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3637-3640.1999
更新日期:1999-07-01 00:00:00
abstract::A system is described for studying adoptive immunity to tuberculosis in syngeneic mice. Donor mice were immunized with 10(4) BCG intravenously, and lymphoid cells were harvested 28 days later. Adoptive immunity was measured in recipient mice in terms of the inhibition of growth of BCG in the liver and spleen following...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.6.1174-1181.1975
更新日期:1975-06-01 00:00:00
abstract::Uropathogenic Escherichia coli strain J96 carries multiple determinants for fimbrial adhesins. The regulatory protein PapB of P fimbriae has previously been implicated in potential coregulatory events. The focB gene of the F1C fimbria determinant is highly homologous to papB; the translated sequences share 81% identit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01010-07
更新日期:2008-02-01 00:00:00
abstract::One- to three-day-old guinea pigs were inoculated intranasally with the chlamydial agent of guinea pig inclusion conjunctivitis. Physical signs of infection included a marked increase in respiration rate on days 5 to 10 of infection and radiographic evidence of pneumonia on day 6. When animals were killed at various t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.1.153-158.1985
更新日期:1985-04-01 00:00:00