Abstract:
:White-footed mice (Peromyscus leucopus), the primary reservoir for Borrelia burgdorferi in the northern midwest and northeastern United States, were experimentally inoculated with an infectious strain or a noninfectious strain of the Lyme disease spirochete and examined for their specific antibody response with the enzyme-linked immunosorbent assay and Western blot (immunoblot) analysis. Immunoglobulin M (IgM) anti-B. burgdorferi antibodies were detected in mice 1 to 2 days after inoculation with either the infectious or noninfectious strain of spirochetes and peaked on days 4 and 5. Mice inoculated with the infectious strain of spirochete had a secondary increase in IgM 21 days after inoculation. Mice also produced both IgG1 and IgG2 antibodies beginning 5 to 7 days after inoculation and they increased in titer until 84 days after inoculation when the experiment was terminated. Western blot analysis of sequential plasma samples from mice inoculated with the infectious strain of spirochete demonstrated the development of IgM, IgG1, and IgG2 antibodies to numerous spirochetal antigens, whereas mice inoculated with the noninfectious strain had reduced blot patterns with antibodies reactive primarily to the 31,000-kilodalton outer surface protein A. Persistent spirochetal infection in some mice, in spite of a strong and diverse antibody response, deserves further investigation.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Schwan TG,Kime KK,Schrumpf ME,Coe JE,Simpson WJdoi
10.1128/IAI.57.11.3445-3451.1989subject
Has Abstractpub_date
1989-11-01 00:00:00pages
3445-51issue
11eissn
0019-9567issn
1098-5522journal_volume
57pub_type
杂志文章abstract::It was demonstrated that complement-coated sheep erythrocytes bind to Candida albicans cells grown in serum-free RPMI 1640 medium. Testing of purified complement components proved that iC3b and C3d were responsible for the reaction, whereas C3b and C3b-H reacted only slightly if at all. Binding occurred only to C. alb...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.598-600.1985
更新日期:1985-11-01 00:00:00
abstract::Vibrio cholerae responds to environmental changes by altering the protein composition of its outer membrane. In rich medium, V. cholerae expresses almost exclusively the outer membrane porin OmpU, whereas in minimal medium, OmpT is the dominant porin. The supplementation of a minimal medium with a mixture of asparagin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05851-11
更新日期:2012-02-01 00:00:00
abstract::Glaesserella (Haemophilus) parasuis is a commensal bacterium of the upper respiratory tract in pigs and also the causative agent of Glässer's disease, which causes significant morbidity and mortality in pigs worldwide. Isolates are characterized into 15 serovars by their capsular polysaccharide, which has shown a corr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00879-19
更新日期:2020-04-20 00:00:00
abstract::In the progression of the life cycle of Plasmodium falciparum, a small proportion of asexual parasites differentiate into male or female sexual forms called gametocytes. Just like their asexual counterparts, gametocytes are contained within the infected host's erythrocytes (RBCs). However, unlike their asexual partner...
journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.00775-18
更新日期:2019-05-21 00:00:00
abstract::Natural bactericidal resistance to Mycobacterium bovis BCG is under the control of a single gene, designated Bcg. Lung granuloma formation in susceptible (Bcgs) and resistant (Bcgr) mice was studied in two sets of Bcg-congenic systems, namely, the BALB/c (Bcgs)-C.D2 (BALB/c.Bcgr) pair and the B10.A (Bcgs)-B10.Ar (Bcgr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1210-1216.1990
更新日期:1990-05-01 00:00:00
abstract::Resistance against acute and chronic infection with Taxoplasma gondii in BALB/c and CBA/Ca mice was compared. Intraperitoneal inoculation of either 20, 40, or 80 cysts of the ME49 strain resulted in mortality rates in BALB/c mice of 12% (2 of 17), 50% (6 of 12), and 75% (9 of 12), respectively, within 3 weeks after in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2284-2288.1993
更新日期:1993-06-01 00:00:00
abstract::Although there appears to be little if any role for specific antibodies in protection against intracellular bacteria, such as the model pathogen F. tularensis live vaccine strain (LVS), the role of B cells themselves in primary and secondary infection with such bacteria has not been examined directly. We show here tha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.6002-6007.1999
更新日期:1999-11-01 00:00:00
abstract::Using fluorescent antibody techniques (FA) and light microscopy (LM) and electron microscopy (EM), this paper describes the morphological features of the ileum in the DK1 mouse orally challenged with adenovirus K87. At the peak of infection, virus is easily identified by FA in the epithelium of the villi and crypts of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.2.569-580.1976
更新日期:1976-02-01 00:00:00
abstract::Bovine sera for tissue culture use were shown to contain heterophil antigen of the Paul-Bunnell-Davidsohn type by immune adherence hemagglutination tests with sera from patients with infectious mononucleosis. The antibody titers observed were comparable to those determined by two other methods; i.e., the immune adhere...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.923-927.1978
更新日期:1978-03-01 00:00:00
abstract::Chlorphenesin (3-p-chlorophenoxy-1,2-propanediol), when injected intravenously together with either of two common bacterial antigens, inhibits the antibody response of the rabbit. The antigens studied are those common to Enterobacteriaceae and to gram-positive bacteria. The immunosuppression is contingent upon incubat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.1.60-64.1970
更新日期:1970-07-01 00:00:00
abstract::A major ceramide monohexoside (CMH) was purified from lipidic extracts of Cryptococcus neoformans. This molecule was analyzed by high-performance thin-layer chromatography (HPTLC), gas chromatography coupled with mass spectrometry, and fast atom bombardment-mass spectrometry. The cryptococcal CMH is a beta-glucosylcer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.7049-7060.2000
更新日期:2000-12-01 00:00:00
abstract::The membrane-damaging properties on human diploid embryonic lung fibroblasts of streptolysin O (from Streptococcus pyogenes) and theta-toxin (from Clostridium perfringens) were compared. The results are consistent with the suggested mechanism for hemolysis by streptolysin O involving one fixation site and one lytic si...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00
abstract::The biological activity of C-ASWS, an alkali-soluble, water-soluble cell wall antigen isolated from mycelial-phase cells of Coccidioides immitis, was compared with that of a commercial coccidioidin (CDN; Cutter Laboratories); CDN-TS, a toluene-induced lysate of mycelial-phase cells; and spherulin, a spherule-derived e...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.3.751-755.1977
更新日期:1977-03-01 00:00:00
abstract::Platelet microbicidal proteins (PMPs) are small, cationic peptides which possess potent microbicidal activities against common bloodstream pathogens, such as Staphylococcus aureus. We previously showed that S. aureus strains exhibiting resistance to thrombin-induced PMP (tPMP-1) in vitro have an enhanced capacity to c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3548-3553.2000
更新日期:2000-06-01 00:00:00
abstract::Vibrio cholerae causes human infection through ingestion of contaminated food and water, leading to the devastating diarrheal disease cholera. V. cholerae forms matrix-encased aggregates, known as biofilms, in the native aquatic environment. While the formation of V. cholerae biofilms has been well studied, little is ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02617-14
更新日期:2015-01-01 00:00:00
abstract::Pneumonia due to Gram-negative bacteria is associated with high mortality. Acinetobacter baumannii is a Gram-negative bacterium that is associated with hospital-acquired and ventilator-associated pneumonia. Bacteria have been described to release outer membrane vesicles (OMVs) that are capable of mediating systemic in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00243-19
更新日期:2019-08-21 00:00:00
abstract::Group B streptococci (GBS) are the major cause of neonatal pneumonia, sepsis, and meningitis. Steps considered to be important in the pathogenesis of this infection include colonization of the rectum and vagina of the mother, aspiration of GBS into the fetal lung during or just prior to delivery, and invasion of GBS i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.6.2450-2458.1994
更新日期:1994-06-01 00:00:00
abstract::Scrub typhus immunogens were prepared by exposing infected yolk sac suspensions of Rickettsia tsutsugamushi to various doses of gamma radiation. Mouse lethality was abolished at doses greater than 200 krads, whereas immunogenicity of the suspensions, as shown by mouse protection tests, was diminished relatively little...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.124-131.1977
更新日期:1977-01-01 00:00:00
abstract::The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor involved in controlling several aspects of immune responses, including the activation and differentiation of specific T cell subsets and antigen-presenting cells, thought to be relevant in the context of experimental Trypanosoma cruzi infec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00575-16
更新日期:2016-09-19 00:00:00
abstract::Urogenital Chlamydia serovars replicating in reproductive epithelium pose a unique challenge to host immunity and vaccine development. Previous studies have shown that CD4 T cells are necessary and sufficient to clear primary Chlamydia muridarum genital tract infections in the mouse model, making a protective CD4 T ce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06339-11
更新日期:2012-06-01 00:00:00
abstract::Intestinal segmented filamentous bacteria (SFB) protect from ameba infection, and protection is transferable with bone marrow dendritic cells (BMDCs). SFB cause an increase in serum amyloid A (SAA), suggesting that SAA might mediate SFB's effects on BMDCs. Here we further explored the role of bone marrow in SFB-mediat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00316-16
更新日期:2016-09-19 00:00:00
abstract::Thirty-two clinical isolates of Shiga-like toxin (SLT)-producing Escherichia coli associated with single cases or outbreaks of bloody diarrhea, hemorrhagic colitis, the hemolytic uremic syndrome, or edema disease of swine were examined for multiple copies of genes belonging to the slt-I or slt-II toxin families. Five ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.1065-1073.1991
更新日期:1991-03-01 00:00:00
abstract::Three strains of Lactobacillus, identified as Lactobacillus acidophilus, Lactobacillus gasseri, and Lactobacillus jensenii, were selected from among 70 isolates from the vaginas of healthy premenopausal women for properties relevant to mucosal colonization or antagonism. All three self-aggregated and adhered to epithe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.1985-1989.1998
更新日期:1998-05-01 00:00:00
abstract::A major virulence factor of group A streptococci is M protein, a surface-exposed fibrillar molecule of which there exist more than 80 distinct serological types. Antigenic variability resides largely in the amino-terminal region of M protein, whereas the carboxy-terminal half of the molecule is highly conserved among ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2666-2672.1988
更新日期:1988-10-01 00:00:00
abstract::Individuals with sickle cell anemia are subject to serious infections caused by a number of bacteria, including Salmonella species and Staphylococcus aureus. It has been suggested that in sickle cell anemia, extensive erythrophagocytosis by macrophages may interfere with their antibacterial function and thereby predis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.3.917-923.1983
更新日期:1983-06-01 00:00:00
abstract::A monoclonal antibody (MAb) was raised against Shiga toxin 2 (Stx2) of Escherichia coli O157:H7. MAb VTm1.1 belonged to the immunoglobulin G1 subclass and had a kappa light chain, and it could neutralize the cytotoxic activity of Stx2 and variants derived from patient strains but not that of variants derived from anim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.5717-5722.1999
更新日期:1999-11-01 00:00:00
abstract::Initiation of a gonococcal infection involves attachment of Neisseria gonorrhoeae to the plasma membrane of an epithelial cell in the mucosal epithelium and its internalization, transepithelial trafficking, and exocytosis from the basal membrane. Piliation and expression of certain Opa proteins and the immunoglobulin ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.896-905.2000
更新日期:2000-02-01 00:00:00
abstract::Histoplasma capsulatum induces a cell-mediated immune response in the lungs and lymphoid organs of mammals. In this study, we analyzed the progression of the cytokine and inflammatory reactions in the lungs of mice infected intranasally with H. capsulatum. We measured cytokine mRNA levels and determined the inflammato...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1473-1481.1998
更新日期:1998-04-01 00:00:00
abstract::The chemoattraction of Propionibacterium acnes lipase for neutrophils and the effect of lipase inhibitor and two antibiotic agents on the chemotaxis were evaluated. Of the various fractions tested, partially purified lipase (fraction 2c) was the most active cytotaxin produced by P. acnes. Serum mediators were not requ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.71-78.1982
更新日期:1982-01-01 00:00:00
abstract::During bacterial infection of the bovine mammary gland, large numbers of leukocytes migrate into the udder, resulting in the establishment of a host response against the pathogen. Currently, the specific leukocyte populations mediating this immune response are not well defined. In the studies described here, we analyz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00