Abstract:
:Host-adapted Gram-negative bacterial pathogens from the Pasteurellaceae, Neisseriaceae, and Moraxellaceae families normally reside in the upper respiratory or genitourinary tracts of their hosts and rely on utilizing iron from host transferrin (Tf) for growth and survival. The surface receptor proteins that mediate this critical iron acquisition pathway have been proposed as ideal vaccine targets due to the critical role that they play in survival and disease pathogenesis in vivo. In particular, the surface lipoprotein component of the receptor, Tf binding protein B (TbpB), had received considerable attention as a potential antigen for vaccines in humans and food production animals but this has not translated into the series of successful vaccine products originally envisioned. Preliminary immunization experiments suggesting that host Tf could interfere with development of the immune response prompted us to directly address this question with site-directed mutant proteins defective in binding Tf. Site-directed mutants with dramatically reduced binding of porcine transferrin and nearly identical structure to the native proteins were prepared. A mutant Haemophilus parasuis TbpB was shown to induce an enhanced B-cell and T-cell response in pigs relative to native TbpB and provide superior protection from infection than the native TbpB or a commercial vaccine product. The results indicate that binding of host transferrin modulates the development of the immune response against TbpBs and that strategies designed to reduce or eliminate binding can be used to generate superior antigens for vaccines.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Frandoloso R,Martínez-Martínez S,Calmettes C,Fegan J,Costa E,Curran D,Yu RH,Gutiérrez-Martín CB,Rodríguez-Ferri EF,Moraes TF,Schryvers ABdoi
10.1128/IAI.02572-14subject
Has Abstractpub_date
2015-03-01 00:00:00pages
1030-8issue
3eissn
0019-9567issn
1098-5522pii
IAI.02572-14journal_volume
83pub_type
杂志文章abstract::Immunoglobulin G (IgG) antibodies against hepatitis B surface antigen were found to be restricted to subclasses IgG1 and IgG3 in serum samples of 17 individuals. Quantitative determinations showed that in 10 samples the minor serum subclass IgG3 contained more antibodies than the predominant subclass IgG1. Ranges of c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.565-568.1983
更新日期:1983-02-01 00:00:00
abstract::l-Arginine (1 M) eluted measles virus adsorbed on monkey erythrocytes without affecting the infectious and hemagglutinating activities of the virus. This phenomenon was also observed for encephalomyocarditis virus and Newcastle disease virus. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.6.1407-1408.1975
更新日期:1975-06-01 00:00:00
abstract::The importance of bacterial phospholipases during serum-mediated killing of Escherichia coli was examined by using wild-type DR+ DS+ and an isogenic phospholipase-deficient mutant DR- DS-. No difference in serum sensitivity was observed when the parental DR+ DS+ and mutant DR- DS- strains were exposed to various conce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.1.183-188.1977
更新日期:1977-10-01 00:00:00
abstract::The genes of the lic1 operon (lic1A to lic1D) are responsible for incorporation of phosphocholine (PCho) into the lipopolysaccharide (LPS) of Haemophilus influenzae. PCho plays a multifaceted role in the commensal and pathogenic lifestyles of a range of mucosal pathogens, including H. influenzae. Structural studies of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00748-07
更新日期:2008-02-01 00:00:00
abstract::The yields of exotoxin A in Pseudomonas aeruginosa cultures were influenced by the concentration of iron in the culture media. When the iron concentration of the culture media was increased from 0.05 to 1.5 microgram/ml, there was at least a 90% decrease in exotoxin A (measured both by enzymatic activity and by mouse ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.785-791.1978
更新日期:1978-03-01 00:00:00
abstract::Escherichia coli F-18, isolated from the feces of a healthy human in 1977, is an excellent colonizer of the streptomycin-treated mouse large intestine and displays normal motility and chemotaxis ability. A chemotaxis-defective derivative of E. coli F-18, E, coli F-18 CheA-, and a nonflagellated derivative, E. coli F-1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.2957-2961.1990
更新日期:1990-09-01 00:00:00
abstract::The gonococcal pilus is a primary virulence factor, providing the initial attachment of the bacterial cell to human mucosal tissues. Pilin, the major subunit of the pilus, can carry a wide spectrum of primary amino acid sequences which are generated by the action of a complex antigenic variation system. Changes in the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.1918-1927.1998
更新日期:1998-05-01 00:00:00
abstract::Wild-type Vibrio cholerae of both El Tor and classical biotypes (strains N16961 and 395, respectively) and nonmotile mutant derivatives with and without flagellar structures were characterized in three different animal models: (i) the rabbit ileal loop, (ii) the removable intestinal tie adult rabbit diarrhea (RITARD) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2727-2736.1991
更新日期:1991-08-01 00:00:00
abstract::Otitis media developed in 67% of chinchillas inoculated intranasally with type 7 Streptococcus pneumoniae and influenza A virus. Only 4% of chinchillas inoculated with influenza alone and 21% of chinchillas inoculated with S. pneumoniae alone developed otitis media. Among the chinchillas that developed otitis media af...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract::Mouse peritoneal macrophages were successfully infected with two strains of Junin virus producing high titers with no apparent cell damage. Infected cultures survived longer than noninfected cultures. The pattern of virus release suggested a persistent infection. Virus replication was delayed in macrophages from mice ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.356-358.1982
更新日期:1982-01-01 00:00:00
abstract::C4BP (C4b-binding protein) is a high-molecular-weight plasma protein that inhibits the classical pathway of complement activation. Recent experiments have demonstrated that C4BP binds to many strains of the gram-positive bacterium Streptococcus pyogenes, a major respiratory tract pathogen. Binding to S. pyogenes was s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3638-3643.1997
更新日期:1997-09-01 00:00:00
abstract::Live attenuated bacteria hold great promise as multivalent mucosal vaccines against a variety of pathogens. A major challenge of this approach has been the successful delivery of sufficient amounts of vaccine antigens to adequately prime the immune system without overattenuating the live vaccine. Here we used a live a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02443-14
更新日期:2015-01-01 00:00:00
abstract::Chlamydia pneumoniae is a causative agent for many respiratory infections and has been associated with cardiovascular diseases in humans. The pathogenicity of C. pneumoniae is thought to depend on its ability to cause persistent infection and to evade host defense. Genome sequence analysis indicates that C. pneumoniae...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.1.345-349.2002
更新日期:2002-01-01 00:00:00
abstract::Our previous animal studies showed that maternal Porphyromonas gingivalis infection in a subcutaneous chamber is associated with hepatic and uterine translocation, as well as systemic induction of maternal inflammatory responses, both of which were associated with fetal growth restriction (FGR). However, P. gingivalis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5163-5168.2003
更新日期:2003-09-01 00:00:00
abstract::Chagas' disease is a chronic infection caused by Trypanosoma cruzi and represents an important public health burden in Latin America. Frequently the disease evolves undetectable for decades, while in a significant fraction of the affected individuals it culminates in death by heart failure. Here, we describe a novel m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2350-2357.2004
更新日期:2004-04-01 00:00:00
abstract::A protein factor which induces high levels of gamma interferon (IFN-gamma) in resting splenic nonadherent cells was isolated from the sera of mice with generalized inflammation caused by endotoxic shock. The factor was highly purified by ammonium sulfate precipitation followed by ion-exchange column chromatography on ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.64-70.1993
更新日期:1993-01-01 00:00:00
abstract::The ability of a pathogen to evade neutrophil phagocytic killing mechanisms is critically important for dissemination and establishment of a systemic infection. Understanding how pathogens overcome these innate defenses is essential for the development of optimal therapeutic strategies for invasive infections. CpsY is...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00027-12
更新日期:2012-05-01 00:00:00
abstract::Raising the magnesium level from 0.4 to 1.5 mM in a medium containing only amino acids (2.08%), salts, and vitamins increased enterotoxin B production by Staphylococcus aureus S-6 by about 80%. The level of phosphates in the medium was lowered to one-tenth (to 2.87 mM) the original amount without adversely affecting g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.158-160.1978
更新日期:1978-04-01 00:00:00
abstract::During the process of phagocytosis, human leukocytes emit a burst of luminescence which can be measured in a liquid scintillation spectrometer. The enzyme superoxide dismutase, which removes superoxide anions (O(2.)), inhibited this chemiluminescence by 70% at a concentration of 100 mug/ml. The enzyme did not inhibit ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1974-06-01 00:00:00
abstract::Enterococcus faecalis strains are resident intestinal bacteria associated with invasive infections, inflammatory bowel diseases, and colon cancer. Although factors promoting E. faecalis colonization of intestines are not fully known, one implicated pathway is a phosphotransferase system (PTS) in E. faecalis strain OG1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00080-19
更新日期:2019-06-20 00:00:00
abstract::A Gram-negative, filamentous, rod-shaped bacillus which failed to grow in cell-free media was isolated in apparently pure culture from the bronchial scraping and washing of a laboratory rat suffering from chronic respiratory disease by inoculating embryonated chicken eggs via the allantoic route. None of the embryos d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.2.472-479.1985
更新日期:1985-02-01 00:00:00
abstract::Neisseria meningitidis is a human-specific pathogen and leading cause of meningitis and septicemia. Factor H binding protein (fHbp), a virulence factor which protects N. meningitidis from innate immunity by binding the human complement regulator factor H (fH) with high affinity, is also a key antigen in vaccines being...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01183-13
更新日期:2014-03-01 00:00:00
abstract::Virulent strains of Yersinia enterocolitica and their plasmidless, avirulent derivatives were examined for their cell surface properties. Increased surface charge and hydrophobicity of Y. enterocolitica were found to be associated with the possession of a 40- to 48-megadalton plasmid. These surface properties were exp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.2.540-543.1984
更新日期:1984-05-01 00:00:00
abstract::The mouse humoral immune response toward native or detergent-extracted outer membrane vesicles (NOMVs and DOMVs, respectively) from Neisseria meningitidis was determined after intranasal immunization. Both preparations elicited high frequencies of NOMV-specific antibody-forming cells (AFCs) locally in the nasal associ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.2528-2537.2004
更新日期:2004-05-01 00:00:00
abstract::Toxic shock syndrome (TSS) toxin 1 (TSST1) is produced by strains of Staphylococcus aureus associated with TSS. Purified TSST1 induces in rabbits a shock-like illness with many features similar to TSS in humans. These symptoms were also induced by TSST1-producing bacteria in diffusion chambers implanted in the rabbit ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.304-309.1985
更新日期:1985-10-01 00:00:00
abstract::The pili of Serratia marcescens US5 isolated from a patient with urinary tract infection were purified and characterized. During the aeration culture, the pili were detached from the bacteria and were precipitated by the addition of ammonium sulfate. The purification of the pili was carried out by ion-exchange chromat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.295-300.1984
更新日期:1984-11-01 00:00:00
abstract::A fibronectin-binding protein (FnBP) adhesin of Staphylococcus aureus possesses three 37- or 38-amino-acid motifs (D1, D2, and D3) that can each bind fibronectin (Fn) with low affinity and that in tandem comprise D1-3, a high-affinity Fn-binding domain. To identify epitopes for the generation of adhesion-blocking anti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.537-543.1997
更新日期:1997-02-01 00:00:00
abstract::In C3H gnotobiotic mice, no significant difference was observed between the translocation of nine clinical isolates of enteropathogenic Campylobacter spp. and that of seven nonisogeneic Escherichia coli strains with or without various determinants of pathogenicity. In addition, there was no correlation found between t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.4.1019-1021.1987
更新日期:1987-04-01 00:00:00
abstract::The influence of the leukotoxin of Pasteurella haemolytica on the generation of arachidonic acid metabolites by bovine polymorphonuclear leukocytes (PMNs) was investigated. PMNs released 5-, 12-, and 15-hydroxyeicosatetraenoic acids (5-, 12-, and 15-HETE) and leukotriene B4 (LTB4) upon stimulation with arachidonic aci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3238-3243.1992
更新日期:1992-08-01 00:00:00
abstract::The two-component regulatory system, GraRS, appears to be involved in staphylococcal responses to cationic antimicrobial peptides (CAPs). However, the mechanism(s) by which GraRS is induced, regulated, and modulated remain undefined. In this study, we used two well-characterized MRSA strains (Mu50 and COL) and their r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05669-11
更新日期:2012-01-01 00:00:00