Abstract:
:The influence of the leukotoxin of Pasteurella haemolytica on the generation of arachidonic acid metabolites by bovine polymorphonuclear leukocytes (PMNs) was investigated. PMNs released 5-, 12-, and 15-hydroxyeicosatetraenoic acids (5-, 12-, and 15-HETE) and leukotriene B4 (LTB4) upon stimulation with arachidonic acid. The leukotoxin preparations dose dependently enhanced the release of the 5-lipoxygenase products 5-HETE and LTB4 in arachidonic acid-stimulated PMNs, whereas the release of 12- and 15-HETE was not affected. The enhanced release of LTB4 and 5-HETE was not due to a decreased cellular retention of the 5-lipoxygenase products. In addition, leukotoxin preparations by themselves were also able to induce LTB4 and 5-HETE production in the absence of exogenous arachidonic acid. Generation of 5-lipoxygenase products by PMNs stimulated by leukotoxin may represent an important cellular event that occurs during infections with P. haemolytica.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Henricks PA,Binkhorst GJ,Drijver AA,Nijkamp FPdoi
10.1128/IAI.60.8.3238-3243.1992keywords:
subject
Has Abstractpub_date
1992-08-01 00:00:00pages
3238-43issue
8eissn
0019-9567issn
1098-5522journal_volume
60pub_type
杂志文章abstract::An enterotoxic factor isolated from cultures of Salmonella yielded reproducible results in the suckling mouse model in contrast to other animal models. The enterotoxin appears to possess properties similar to both the heat-stable and heat-labile enterotoxins of Escherichia coli. Preliminary results indicate that the t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.14-22.1975
更新日期:1975-01-01 00:00:00
abstract::Mice were vaccinated with 300 micrograms of BCG cell walls (BCG-CW) in oil-in-water emulsion intravenously or with a high or low dose of living BCG by inhalation (BCG-HD or BCG-LD, respectively). The consequences of vaccination were evaluated in terms of the growth of BCG in the lungs and spleen, lung and spleen weigh...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Mycoplasma gallisepticum is an avian respiratory and reproductive tract pathogen that has a significant economic impact on the poultry industry worldwide. Although membrane proteins of Mycoplasma spp. are thought to play crucial roles in host interactions, very few have had their biochemical function defined. In this ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00248-19
更新日期:2019-08-21 00:00:00
abstract::Many of the virulence factors associated with fulminant group A streptococci (GAS) infection are expressed under in vitro exponential growth conditions. However, the survival of GAS in tissue and intracellularly, as well as colonization of asymptomatic carriers, has been reported for GAS. The bacteria associated with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.8.3841-3847.1998
更新日期:1998-08-01 00:00:00
abstract::The adherence of 58 nontypeable Haemophilus influenzae isolates obtained from patients with otitis media or chronic obstructive pulmonary disease (COPD) and obtained from the throats of healthy individuals to Chang and NCI-H292 epithelial cells was compared. Otitis media isolates, but not COPD isolates, adhered signif...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.8.4658-4665.2000
更新日期:2000-08-01 00:00:00
abstract::The superoxide anion (O2-)-generating activity of guinea pig macrophages stimulated by wheat germ agglutinin (WGA), immune complexes, or phorbol myristate acetate (PMA) was studied after short- and long-term exposures of the cells to muramyl dipeptide (MDP) or lipopolysaccharide (LPS). Neither MDP nor LPS alone induce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.82-86.1984
更新日期:1984-07-01 00:00:00
abstract::In the murine model of urinary tract infections (UTI), cystitis by uropathogenic Escherichia coli (UPEC) occurs through an intimate relationship with the bladder superficial umbrella cell entailing cycles of adherence, invasion, intracellular bacterial community (IBC) formation, and dispersal (fluxing) from the intrac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7657-7668.2005
更新日期:2005-11-01 00:00:00
abstract::Chronic lung infections with Pseudomonas aeruginosa biofilms are associated with refractory and fatal pneumonia in cystic fibrosis (CF). In this study, a group of genomically diverse P. aeruginosa isolates were compared with the reference strain PAO1 to assess the roles of motility, twitching, growth rate, and overpro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.1.133-144.2004
更新日期:2004-01-01 00:00:00
abstract::We have previously shown that malaria parasites liberate exoantigens which, through a phospholipid component, stimulate mouse macrophages to secrete tumor necrosis factor (TNF), which are toxic to D-galactosamine-sensitized mice, and which therefore might be involved in pathology. Plasmodium yoelii exoantigens detoxif...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.5.1894-1901.1992
更新日期:1992-05-01 00:00:00
abstract::Complement-fixing antibodies were first detected in mice 7 days after intravenous injection with Mycoplasma arthritidis. Peak titers were observed at 21 days, and high levels of antibody persisted through 293 days. The metabolism-inhibiting antibody response was minimal. On fractionation of mouse sera, only 7S antibod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.4.431-440.1971
更新日期:1971-10-01 00:00:00
abstract::Escherichia coli is a major cause of enteric/diarrheal diseases, urinary tract infections, and sepsis. E. coli K1 is the leading gram-negative organism causing neonatal meningitis, but the microbial basis of E. coli K1 meningitis is incompletely understood. Here we employed comparative genomic hybridization to investi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.4.2196-2206.2006
更新日期:2006-04-01 00:00:00
abstract::Polymorphonuclear leukocytes (PMN) from patients with chronic granulomatous disease (CGD) fail to produce microbicidal concentrations of reactive oxygen species (ROS) due to mutations in NOX2. Patients with CGD suffer from severe, life-threatening infections and inflammatory complications. Granulibacter bethesdensis i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00778-15
更新日期:2015-11-01 00:00:00
abstract::A 60-kilodalton (kDa) immunodominant antigen of Legionella pneumophila is a heat shock protein (HSP) of the GroEL class of HSPs. The gene (htpB) coding the 60-kDa protein was localized to a 3.2-kilobase DNA fragment of L. pneumophila cloned into pUC19 (pSH16) (P. S. Hoffman, C. A. Butler, and F. D. Quinn, Infect. Immu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.10.3380-3387.1990
更新日期:1990-10-01 00:00:00
abstract::We have investigated the roles of gamma interferon (IFN-gamma) and interleukin-4 (IL-4) in host defense against Brugia malayi. Our data suggest that the lack of either IFN-gamma or IL-4 prolongs the time required to achieve sterile immunity, suggesting that both canonical type 1 and type 2 responses are involved in th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.3034-3035.2000
更新日期:2000-05-01 00:00:00
abstract::A cysteine-containing peptide motif, EWSPCSVTCG, is found highly conserved in the circumsporozoite protein (CSP) and the thrombospondin-related anonymous protein (TRAP) of all the Plasmodium species analyzed so far and has been shown to be crucially involved in the sporozoite invasion of hepatocytes. We have recently ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3232-3241.1998
更新日期:1998-07-01 00:00:00
abstract::Little is known about Zn homeostasis in Yersinia pestis, the plague bacillus. The Znu ABC transporter is essential for zinc (Zn) uptake and virulence in a number of bacterial pathogens. Bioinformatics analysis identified ZnuABC as the only apparent high-affinity Zn uptake system in Y. pestis. Mutation of znuACB caused...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00732-10
更新日期:2010-12-01 00:00:00
abstract::Escherichia coli strains expressing the K1 capsule are a major cause of sepsis and meningitis in human neonates. The development of these diseases is dependent on the expression of a range of virulence factors, many of which remain uncharacterized. Here, we show that all but 1 of 34 E. coli K1 neonatal isolates carrie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00716-15
更新日期:2015-09-01 00:00:00
abstract::Microbial survival in a host is usually dependent on the ability of a pathogen to undergo changes that promote escape from host defense mechanisms. The human-pathogenic fungus Cryptococcus neoformans undergoes phenotypic switching in vivo that promotes persistence in tissue. By microarray and real-time PCR analyses, t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01079-08
更新日期:2009-01-01 00:00:00
abstract::Infection of C57BL/6 mice with the third-stage larvae of Brugia pahangi results in a rapid expansion of NK1.1(+) T cells in the spleen and draining lymph nodes. NK T cells produced interleukin-4 in the spleen within 24 h of infection, and these cells were CD4(-). ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.2215-2219.2002
更新日期:2002-04-01 00:00:00
abstract::Intracellular multiplication of virulent Legionella pneumophila serogroup 1 was inhibited by human monocyte-derived macrophages activated by a glycoprotein extract of Klebsiella pneumoniae, RU 41.740. Macrophage cultures were infected with L. pneumophila in the presence of immune antibodies on day 7 of culture. Extrac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2234-2239.1987
更新日期:1987-09-01 00:00:00
abstract::Cryptococcus neoformans laccase expression during murine infection was investigated in lung tissue by immunohistochemistry and immunogold electron microscopy. Laccase was detected in the fungal cell cytoplasm, cell wall, and capsule in vivo. The amount of laccase found in different sites varied as a function of the ti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.3124-3127.2005
更新日期:2005-05-01 00:00:00
abstract::Chlamydial inclusions were demonstrated by indirect immunofluorescence (IF) with antiserum to the chlamydial group antigen when McCoy cell monolayers infected with either Chlamydia trachomatis or Chlamydia psittaci were fixed in formaldehyde or paraformaldehyde, provided the monolayer was not allowed to dry. If these ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.561-570.1981
更新日期:1981-11-01 00:00:00
abstract::Monoclonal antibodies secreted by six hybridomas and recognizing antigenic sites on glycoproteins gC, gAB, gD, gE, and gF of herpes simplex virus type 2 were examined for their ability to protect BALB/c mice from lethal infection by the virus. Administration of monoclonal antibodies to individual glycoproteins intrape...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.1132-1137.1982
更新日期:1982-09-01 00:00:00
abstract::An important role for immunoglobulin M (IgM) during early acute virulent Toxoplasma gondii infection was identified using IgM-/- mice that lack surface and secretory IgM but maintain normal B-cell functionality and isotype class switching. Following intraperitoneal inoculation with the virulent RH strain, IgM-/- mice ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.8060-8068.2005
更新日期:2005-12-01 00:00:00
abstract::Previous studies have demonstrated that both Ixodes scapularis saliva and Borrelia burgdorferi antigens modulated lymphokines and monokines in vitro. The studies presented here were designed to delineate the role of I. scapularis and B. burgdorferi in modulation of the host immune response in vivo. Infestation of C3H/...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.8.3100-3106.1997
更新日期:1997-08-01 00:00:00
abstract::Synthetic peptides and murine monoclonal antibodies were used to map cross-reactive chlamydial epitopes. A species-specific epitope in the central region of variable sequence region 4 abuts the amino-terminal end of a B-serogroup-specific or F/G-serogroup-specific epitope, which in turn abuts known serovar-specific ep...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2839-2841.1996
更新日期:1996-07-01 00:00:00
abstract::Shiga-like toxins (SLTs), which are produced by certain strains of Escherichia coli, are composed of enzymatically active A and B subunit multimers responsible for the toxin's binding. We have previously purified large amounts of the SLT-I B subunit by using a hyperexpression vector in Vibrio cholerae under the contro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.1.301-308.1995
更新日期:1995-01-01 00:00:00
abstract::The screening of several inbred strains of mice suggested that the capacity of their spleen cells to respond to the mitogenic effect of lipopolysaccharide (LPS) of gram-negative bacteria was correlated with their resistance to intraperitoneal infection with Salmonella typhimurium. An infection of LPS into mice caused ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.26-33.1977
更新日期:1977-01-01 00:00:00
abstract::The role of cysteine proteinases in adherence of Trichomonas vaginalis NYH 286 to HeLa and human vaginal epithelial cells was evaluated. Only pretreatment of trichomonads, but not epithelial cells, with N-alpha-p-tosyl-L-lysine chloromethyl ketone (TLCK), an inhibitor of trichomonad cysteine proteinases, greatly dimin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.2991-2997.1989
更新日期:1989-10-01 00:00:00
abstract::We found that oral immunization with flagellum-defective mutant strains of Salmonella enterica serovar Typhimurium with the ClpXP-deficient background protected mice against oral challenge with the virulent strain. These data indicate that Salmonella flagellin is not a dominant protective antigen in oral immunization ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2449-2451.2004
更新日期:2004-04-01 00:00:00