Abstract:
:The ability of a pathogen to evade neutrophil phagocytic killing mechanisms is critically important for dissemination and establishment of a systemic infection. Understanding how pathogens overcome these innate defenses is essential for the development of optimal therapeutic strategies for invasive infections. CpsY is a conserved transcriptional regulator previously identified as an important virulence determinant for systemic infection of Streptococcus iniae. While orthologs of CpsY have been associated with the regulation of methionine metabolism and uptake pathways, CpsY additionally functions in protection from neutrophil-mediated killing. S. iniae does not alter neutrophil phagosomal maturation but instead is able to adapt to the extreme bactericidal environment of a mature neutrophil phagosome, a property dependent upon CpsY. This CpsY-dependent adaptation appears to involve stabilization of the cell wall through peptidoglycan O-acetylation and repression of cellular autolysins. Furthermore, S. iniae continues to be a powerful model for investigation of bacterial adaptations during systemic streptococcal infection.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Allen JP,Neely MNdoi
10.1128/IAI.00027-12subject
Has Abstractpub_date
2012-05-01 00:00:00pages
1707-15issue
5eissn
0019-9567issn
1098-5522pii
IAI.00027-12journal_volume
80pub_type
杂志文章abstract::Production of interleukin-10 (IL-10) by C57BL/6 mice following infection with Borrelia burgdorferi has been proposed as a mechanism whereby resistance to the development of experimental Lyme arthritis is maintained. In the current study, we sought to determine the role of IL-10 during infection of arthritis- and cardi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00808-08
更新日期:2008-12-01 00:00:00
abstract::Nonopsonic invasion of mononuclear phagocytes by Mycobacterium tuberculosis is likely important in the establishment of a primary infection in the lung. M. tuberculosis binds to a variety of phagocyte receptors, of which the mannose receptor and complement receptor type 3 (CR3) may support nonopsonic binding. CR3, a b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5373-5383.1996
更新日期:1996-12-01 00:00:00
abstract::A murine immunoglobulin G monoclonal antibody (MAb) raised against outer membrane vesicles of Moraxella catarrhalis 035E was shown to bind to a surface-exposed epitope of a major outer membrane protein of this organism. This outer membrane protein, which had an apparent molecular weight of approximately 80,000 in sodi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.2003-2010.1993
更新日期:1993-05-01 00:00:00
abstract::Cryptococcal meningoencephalitis develops as a result of hematogenous dissemination of inhaled Cryptococcus neoformans from the lung to the brain. The mechanism(s) by which C. neoformans crosses the blood-brain barrier (BBB) is a key unresolved issue in cryptococcosis. We used both an in vivo mouse model and an in vit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.4985-4995.2004
更新日期:2004-09-01 00:00:00
abstract::To study the specific role of transmembrane tumor necrosis factor (tmTNF) in protective and pathological responses against the gastrointestinal helminth Trichinella spiralis, we compared the immune responses of TNF-alpha/lymphotoxin alpha (LTalpha)(-/-) mice expressing noncleavable transgenic tmTNF to those of TNF-alp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01461-08
更新日期:2009-09-01 00:00:00
abstract::Spleen cells from BALB/c mice infected with 2 X 10(7) L. major promastigotes and developing progressive disease produced significantly lower levels of interleukin-2 (IL-2) in response to concanavalin A stimulation than did spleen cells from uninfected mice. In contrast, spleen cells from sublethally irradiated and inf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.386-394.1986
更新日期:1986-11-01 00:00:00
abstract::Diverse pathogens have evolved to survive and replicate in the endosomes or phagosomes of the host cells and establish persistent infection. Ehrlichiae are Gram-negative, intracellular bacteria that are transmitted by ticks. Ehrlichiae reside in the endosomes of the host phagocytic or endothelial cells and establish p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02101-14
更新日期:2014-10-01 00:00:00
abstract::The adhesion of K21a, K26, K36, and K50 capsulated Klebsiella strains to ileocecal (HCT-8) and bladder (T24) epithelial cell lines was significantly lower than that of their corresponding spontaneous noncapsulated variants K21a/3, K26/1, K36/3, and K50/3, respectively. Internalization of the bacteria by both epithelia...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6744-6749.2000
更新日期:2000-12-01 00:00:00
abstract::The C-terminal 19-kDa domain of merozoite surface protein 1 (MSP1₁₉) is the target of protective antibodies but alone is poorly immunogenic. Previously, using the Plasmodium yoelii murine model, we fused P. yoelii MSP1₁₉ (PyMSP1₁₉) with full-length P. yoelii merozoite surface protein 8 (MSP8). Upon immunization, the M...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00211-12
更新日期:2012-07-01 00:00:00
abstract::Neuronal dysfunction can occur in the course of sepsis without meningitis. Sepsis-associated neuronal damage (SAND) was observed in the hippocampus within hours in experimental pneumococcal bacteremia. Intravascular challenge with purified bacterial cell wall recapitulated SAND. SAND persisted in PAFr(-/-) mice but wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00022-06
更新日期:2006-07-01 00:00:00
abstract::Smoking is associated with increased susceptibility to tuberculosis and influenza. However, little information is available on the mechanisms underlying this increased susceptibility. Mice were left unexposed or were exposed to cigarette smoke and then infected with Mycobacterium tuberculosis by aerosol or influenza A...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00709-10
更新日期:2011-01-01 00:00:00
abstract::Although protective immunity in C57BL/6 mice induced by a single dose of the radiation-attenuated schistosome vaccine is believed to be mediated by Th1-type immune responses, we here report that in BALB/c mice protection can also depend upon signaling via the interleukin-4 (IL-4) receptor which conventionally governs ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.228-236.2001
更新日期:2001-01-01 00:00:00
abstract::Toll-like receptors (TLRs) are a major component of the pattern recognition receptor repertoire that detect invading microorganisms and direct the vertebrate immune system to eliminate infection. In chickens, the differential biology of Salmonella serovars (systemic versus gut-restricted localization) correlates with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2344-2350.2005
更新日期:2005-04-01 00:00:00
abstract::Establishment and maintenance of chronic lung infections with mucoid Pseudomonas aeruginosa in patients with cystic fibrosis (CF) require that the bacteria avoid host defenses. Elaboration of the extracellular, O-acetylated mucoid exopolysaccharide, or alginate, is a major microbial factor in resistance to immune effe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1895-1901.2001
更新日期:2001-03-01 00:00:00
abstract::Campylobacter jejuni is a leading cause of infectious diarrhea throughout the world. In addition, there is growing evidence that Guillain-Barré syndrome, an inflammatory demyelinating disease of the peripheral nervous system, is frequently preceded by C. jejuni infection. In the present study, the hrcA-grpE-dnaK gene ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1194-1200.1999
更新日期:1999-03-01 00:00:00
abstract::The bundle-forming pilus (BFP) of enteropathogenic Escherichia coli (EPEC) is an important virulence factor. We examined the role of divergent alleles of bfpA encoding bundlin, the BFP pilin protein, in pilus biogenesis, pilus interactions, and immune responses. We found that the BFP biogenesis machine from an EPEC st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00009-07
更新日期:2007-10-01 00:00:00
abstract::Toxin A (TcdA) and toxin B (TcdB) are major virulence factors of Clostridium difficile. These two toxins intoxicate cultured cells by similar mechanisms, and TcdB generally is more potent than TcdA in cultured cells. The exact reason for this difference is unclear. Here, we report that the cellular effects of TcdA can...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01577-08
更新日期:2009-06-01 00:00:00
abstract::G protein-coupled receptor kinase 5 (GRK5) is a serine/threonine kinase previously shown to mediate polymicrobial sepsis-induced inflammation. The goal of the present study was to examine the role of GRK5 in monomicrobial pulmonary infection by using an intratracheal Escherichia coli infection model of pneumonia. We u...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00051-16
更新日期:2016-04-22 00:00:00
abstract::Henle 407 and HeLa cells were compared as hosts for Shigella dysenteriae at a low multiplicity of infection. Efficiency of attachment and invasion without centrifugation, as well as selectivity for pathogenic over nonpathogenic S. dysenteriae without Congo red, were much greater for Henle 407 cells than for HeLa cells...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2401-2403.1990
更新日期:1990-07-01 00:00:00
abstract::A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.3300-3303.2002
更新日期:2002-06-01 00:00:00
abstract::Candida albicans is a major opportunistic pathogen in immunocompromised patients. Production of proinflammatory cytokines by host cells in response to C. albicans plays a critical role in the activation of immune cells and final clearance of the organism. Invasion of host cells and tissues is considered one of the vir...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.4588-4595.2005
更新日期:2005-08-01 00:00:00
abstract::Current immunization strategies, using peptide or protein antigens, generally fail to elicit cytotoxic-T-lymphocyte responses, since these antigens are unable to access intracellular compartments where loading of major histocompatibility complex class I (MHC-I) molecules occurs. In an attempt to circumvent this, we in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.3249-3258.2002
更新日期:2002-06-01 00:00:00
abstract::Alveolar bone destruction is a characteristic feature of periodontitis. Treponema denticola is known to be involved in periodontitis. To elucidate the role of T. denticola in alveolar bone destruction in periodontitis, the effects of lipooligosaccharide (LOS) from T. denticola on osteoclast formation and on expression...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.226-233.2003
更新日期:2003-01-01 00:00:00
abstract::The relative roles of serum factors required for opsonization of type XIV Streptococcus pneumoniae were investigated by means of luminol-enhanced chemiluminescence (CL), bactericidal, and immunofluorescence assays employing adult sera containing high (>1,000 ng of antibody nitrogen per ml) or low (<200 ng of antibody ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.800-808.1982
更新日期:1982-03-01 00:00:00
abstract::The tetraspanins are a superfamily of transmembrane proteins with diverse functions and can form extended microdomains within the plasma membrane in conjunction with partner proteins, which probably includes receptors for bacterial adhesins. Neisseria meningitidis, the causative agent of meningococcal disease, attache...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01354-10
更新日期:2011-06-01 00:00:00
abstract::There are no vaccines against boutonneuse fever and Rocky Mountain spotted fever. Previous studies have identified a Rickettsia rickettsii surface protein as a vaccine candidate and shown that an antigenically related protein is present in R. conorii, which causes boutonneuse fever. The gene encoding the R. rickettsii...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.3.646-653.1990
更新日期:1990-03-01 00:00:00
abstract::A hamster immunization challenge assay described in the accompanying paper (M. F. Barile, D. K. F. Chandler, H. Yoshida, M. W. Grabowski, R. Harasawa, and S. Razin, Infect. Immun. 56:2443-2449, 1988) was used to examine protection against Mycoplasma pneumoniae disease by passive immunization and to evaluate the protec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.9.2450-2457.1988
更新日期:1988-09-01 00:00:00
abstract::Plasmodium falciparum apical membrane antigen 1 (AMA1) is a leading malaria vaccine candidate whose function has not been unequivocally defined. Partial complementation of function can be achieved by exchanging the AMA1 of P. falciparum (PfAMA1) with that of P. chabaudi (PcAMA1). In this study, parasites expressing ch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2444-2451.2005
更新日期:2005-04-01 00:00:00
abstract::We studied interleukin-1 (IL-1) secretion by rat peritoneal exudate macrophages stimulated with purified toxic shock syndrome toxin-1 (TSST-1). TSST-1 was observed to be a more potent inducer of IL-1 than was endotoxin. The induction of IL-1 secretion by TSST-1 was not blocked by polymyxin B but could be blocked by mo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.12.2865-2869.1987
更新日期:1987-12-01 00:00:00
abstract::Spleen cell cultures from guinea pigs given legionella pneumophila vaccine in complete Freund adjuvant or as a sublethal infection were inhibited in their migration activity in vitro when incubated with specific antigen. Both direct and indirect migration inhibition assays revealed sensitization of the guinea pigs to ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.3.1132-1137.1983
更新日期:1983-09-01 00:00:00