DNA microarray assessment of putative Borrelia burgdorferi lipoprotein genes.


:A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. These data show that a DNA microarray can globally examine the genes encoding B. burgdorferi lipoproteins.


Infect Immun


Infection and immunity


Liang FT,Nelson FK,Fikrig E





Has Abstract


2002-06-01 00:00:00












  • Human opsonins induced during meningococcal disease recognize outer membrane proteins PorA and PorB.

    abstract::Human opsonins directed against specific meningococcal outer membrane structures in sera obtained during meningococcal disease were quantified with a recently developed antigen-specific, opsonin-dependent phagocytosis and oxidative burst assay. Outer membrane vesicles (OMVs) and PorA (class 1) and PorB (class 3) prote...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lehmann AK,Halstensen A,Aaberge IS,Holst J,Michaelsen TE,Sornes S,Wetzler LM,Guttormsen H

    更新日期:1999-05-01 00:00:00

  • Lipopolysaccharide from Brucella abortus behaves as a T-cell-independent type 1 carrier in murine antigen-specific antibody responses.

    abstract::In order to determine the carrier nature of lipopolysaccharide from Brucella abortus (LPS-BA) in evoking humoral responses, normal and immunodeficient mice were immunized with trinitrophenyl (TNP)-conjugated LPS-BA (TNP-LPS-BA) and the responses were compared with those to known T-dependent and T-independent antigens....

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Betts M,Beining P,Brunswick M,Inman J,Angus RD,Hoffman T,Golding B

    更新日期:1993-05-01 00:00:00

  • Heterogeneic autoantibody against neurofilament protein in the sera of animals with experimental kuru and Creutzfeldt-Jakob disease and natural scrapie infection.

    abstract::Heterogeneic autoantibodies against axonal neurofilament proteins of mature mouse neurons grown in vitro were detected by the indirect immunofluorescence technique in 12.7% (9 of 71) of the sera from nonhuman primates infected with kuru, in 14.5% (17 of 117) and 4% (1 of 25), respectively, of the sera from nonhuman pr...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Aoki T,Gibbs CJ Jr,Sotelo J,Gajdusek DC

    更新日期:1982-10-01 00:00:00

  • Antibodies to malaria peptide mimics inhibit Plasmodium falciparum invasion of erythrocytes.

    abstract::Apical membrane antigen 1 (AMA1) is expressed on the surfaces of Plasmodium falciparum merozoites and is thought to play an important role in the invasion of erythrocytes by malaria parasites. To select for peptides that mimic conformational B-cell epitopes on AMA1, we screened a phage display library of >10(8) indivi...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Casey JL,Coley AM,Anders RF,Murphy VJ,Humberstone KS,Thomas AW,Foley M

    更新日期:2004-02-01 00:00:00

  • Influence of growth medium on adsorption of Streptococcus mutans, Actinomyces viscosus, and Actinomyces naeslundii to saliva-treated hydroxyapatite surfaces.

    abstract::The influence of the growth medium on the ability of strains of Streptococcus mutans, Actinomyces viscosus and A. naeslundii to attach to saliva-treated hydroxyapatite (S-HA) surfaces was studied. Preliminary experiments indicated that cells of each species harvested in lag, log, and early stationary phases of growth ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Peros WJ,Gibbons RJ

    更新日期:1981-04-01 00:00:00

  • A synthetic glycoconjugate representing the genus-specific epitope of chlamydial lipopolysaccharide exhibits the same specificity as its natural counterpart.

    abstract::The tetrasaccharide 3-deoxy-alpha-D-manno-2-octulosonic acid (alpha-KDO) (2----8)-alpha-KDO(2----4)-alpha-KDO(2----6)-beta GlcNAc, a partial structure of chlamydial lipopolysaccharide (LPS) representing a genus-specific epitope, was synthesized and covalently linked to bovine serum albumin, resulting in an artificial ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fu Y,Baumann M,Kosma P,Brade L,Brade H

    更新日期:1992-04-01 00:00:00

  • Isolation and partial characterization of staphylococcal decomplementation antigen.

    abstract::A substance with potent decomplementation activity was isolated from staphylococcal culture supernatants by polyethylene glycol precipitation, DEAE-ion-exchange and Sephacryl chromatography, and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified substance exhibited all the characterist...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bhakdi S,Muhly M

    更新日期:1985-01-01 00:00:00

  • Exaggerated proinflammatory and Th1 responses in the absence of gamma/delta T cells after infection with Listeria monocytogenes.

    abstract::While gamma/delta T cells are involved in host defense and immunopathology in a variety of infectious diseases, their precise role is not yet clearly defined. In the absence of gamma/delta T cells, mice die after infection with a dose of Listeria monocytogenes that is not lethal in immunologically intact animals. Morb...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Skeen MJ,Rix EP,Freeman MM,Ziegler HK

    更新日期:2001-12-01 00:00:00

  • Effect of temperature on survival and growth of infectious pancreatic necrosis virus.

    abstract::Infectious pancreatic necrosis virus was stable for 10 days at 4 C in stream and well water, after which the virus had a half-life of 7.5 days. At 15 C, the virus was stable for 5 days, and then had a half-life between 5 and 6 days. Viral antigen in infected cells developed much more slowly at 4 C than at 20 C. Infect...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tu KC,Spendlove RS,Goede RW

    更新日期:1975-06-01 00:00:00

  • Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces.

    abstract::Slime production is not a generally recognized feature of Staphylococcus epidermidis. In a recent outbreak of S. epidermidis intravascular catheter-associated sepsis, we noted that 63% of clinically implicated strains grew as a slimy film coating the culture tube walls when propagated in tryptic soy broth. Only 37% of...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Christensen GD,Simpson WA,Bisno AL,Beachey EH

    更新日期:1982-07-01 00:00:00

  • Viral infections in renal transplant recipients.

    abstract::Cytomegalovirus (CMV) infections are common in renal transplant recipients. We studied 23 recipients prospectively to determine whether infections by other herpes-group and non-herpes-group viruses were also present. Sera, obtained at the time of surgery and periodically thereafter, were tested for antibody to CMV, he...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Armstrong JA,Evans AS,Rao N,Ho M

    更新日期:1976-10-01 00:00:00

  • Host inflammatory response inhibits Escherichia coli O157:H7 adhesion to gut epithelium through augmentation of mucin expression.

    abstract::Escherichia coli O157:H7, a major Shiga toxin-producing pathogen, has a low infectious dose and causes serious illness in humans. The gastrointestinal tract of cattle is the primary reservoir of E. coli O157:H7, and thus, it is critical to eliminate or reduce E. coli O157:H7 gut colonization. Given that E. coli O157:H...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Xue Y,Zhang H,Wang H,Hu J,Du M,Zhu MJ

    更新日期:2014-05-01 00:00:00

  • Differential kinetics and distribution of antibodies in serum and nasal and vaginal secretions after nasal and oral vaccination of humans.

    abstract::Although nasal vaccination has emerged as an interesting alternative to systemic or oral vaccination, knowledge is scarce about the immune responses after such immunization in humans. In the present study, we have compared the kinetics and organ distribution of the antibody responses after nasal and oral vaccination. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rudin A,Johansson EL,Bergquist C,Holmgren J

    更新日期:1998-07-01 00:00:00

  • Mechanisms of invasion and replication of the intracellular stage in Trypanosoma cruzi.

    abstract::Amastigotes obtained from spleens of mice infected with different strains of Trypanosoma cruzi were examined for their ability to invade macrophages and L929 cells and to initiate infection in mice. Both types of cells were readily invaded by organisms of the strains Y, MR, and Tulahuen. Organisms of the CL strain wer...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: McCabe RE,Remington JS,Araujo FG

    更新日期:1984-11-01 00:00:00

  • Rickettsial infectious antibody complexes: detection by antiglobulin plaque reduction technique.

    abstract::Rickettsia rickettsii and R. australis formed infectious antibody complexes when reacted with homologous convalescent serum. The addition of antiglobulin to these complexes showed neutralization of plaques proportional to the concentration of rickettsial immune serum. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kenyon RH,McManus AT

    更新日期:1974-05-01 00:00:00

  • Efficient B cell responses to Borrelia hermsii infection depend on BAFF and BAFFR but not TACI.

    abstract::T cell-independent antibody responses develop rapidly, within 3 to 4 days, and are critical for preventing blood-borne pathogens from evolving into life-threatening infections. The interaction of BAFF, also known as BLyS, with its receptors BAFFR and TACI on B cells is critical for B cell homeostasis and function. Usi...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Dickinson GS,Sun G,Bram RJ,Alugupalli KR

    更新日期:2014-01-01 00:00:00

  • Recognition of immunoglobulin A1 by oral actinomyces and streptococcal lectins.

    abstract::Actinomyces naeslundii and Streptococcus gordonii, oral bacteria that possess Gal/GalNAc- and sialic acid-reactive lectins, respectively, were adherent to immobilized secretory immunoglobulin A (IgA) and two IgA1 myeloma proteins but not to two IgA2 myeloma proteins. Apparently, O-linked oligosaccharides at the hinge ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ruhl S,Sandberg AL,Cole MF,Cisar JO

    更新日期:1996-12-01 00:00:00

  • Complement-mediated bactericidal activity of anti-factor H binding protein monoclonal antibodies against the meningococcus relies upon blocking factor H binding.

    abstract::Binding of the complement-downregulating protein factor H (fH) to the surface of the meningococcus is important for survival of the organism in human serum. The meningococcal vaccine candidate factor H binding protein (fHbp) is an important ligand for human fH. While some fHbp-specific monoclonal antibodies (MAbs) blo...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Giuntini S,Reason DC,Granoff DM

    更新日期:2011-09-01 00:00:00

  • Binding of Candida albicans to immobilized amino acids and bovine serum albumin.

    abstract::In this study, we examined the binding of Candida albicans synchronized yeast-phase cells to plastic, immobilized amino acids and bovine serum albumin (BSA) and quantified the binding by using an XTT tetrazolium salt assay and absorbance determination. Our results show that C. albicans binds efficiently and specifical...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hawser SP,Islam K

    更新日期:1998-01-01 00:00:00

  • Immunization with VAR2CSA-DBL5 recombinant protein elicits broadly cross-reactive antibodies to placental Plasmodium falciparum-infected erythrocytes.

    abstract::Pregnancy-associated malaria is a severe clinical syndrome associated with the sequestration of Plasmodium falciparum-infected erythrocytes in the placenta. Placental binding is mediated by VAR2CSA, a member of the large and diverse P. falciparum erythrocyte membrane 1 (PfEMP1) protein family. To better understand if ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Avril M,Cartwright MM,Hathaway MJ,Hommel M,Elliott SR,Williamson K,Narum DL,Duffy PE,Fried M,Beeson JG,Smith JD

    更新日期:2010-05-01 00:00:00

  • Activation of alveolar macrophages exposed to lavage-procured immunoglobulin G obtained from normal rabbit lungs.

    abstract::Pulmonary washings from rabbits were freed of cells and added to the monolayers of homologous alveolar macrophages (AM). At 1 h after incubation with the pulmonary washings, many more cells adhered to glass, spread out, and showed enhanced Nitro Blue Tetrazolium reduction. The maximal effect of the pulmonary washings ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ando M,Suga M,Shima K,Sugimoto M,Tokuomi H

    更新日期:1978-05-01 00:00:00

  • Differential expression and transcriptional analysis of the alpha-2,3-sialyltransferase gene in pathogenic Neisseria spp.

    abstract::Alpha-2,3-sialyltransferase (Lst) is expressed on the outer membrane of Neisseria gonorrhoeae and Neisseria meningitidis and sialylates surface lipooligosaccharide (LOS), facilitating resistance to complement-mediated killing. The enzyme is constitutively expressed from a single gene (lst) and does not undergo antigen...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Packiam M,Shell DM,Liu SV,Liu YB,McGee DJ,Srivastava R,Seal S,Rest RF

    更新日期:2006-05-01 00:00:00

  • Surface hydrophobicity, adherence, and aggregation of cell surface protein antigen mutants of Streptococcus mutans serotype c.

    abstract::The pac gene of the serotype c strain Streptococcus mutans MT8148 encodes a cell surface protein antigen (PAc) of approximate 190 kilodaltons. The serotype c strain S. mutans GS-5 does not produce the 190-kilodalton PAc but produces a lower-molecular-weight protein that reacts with anti-PAc serum. The SphI-BamHI fragm...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Koga T,Okahashi N,Takahashi I,Kanamoto T,Asakawa H,Iwaki M

    更新日期:1990-02-01 00:00:00

  • Choriogonadotropin-like antigen in an anaerobic bacterium, Eubacterium lentum, isolated from a rectal tumor.

    abstract::Using the indirect fluorescein-labeled and indirect peroxidase-antiperoxidase-labeled immunohistochemical techniques, and utilizing both antiserum specific for the beta-subunit of choriogonadotropin and antiserum for the total hormone, we have demonstrated the presence of a choriogonadotropin-like immunoreactive mater...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Acevedo HF,Slifkin M,Pouchet-Melvin GR,Campbell-Acevedo EA

    更新日期:1979-06-01 00:00:00

  • Comparison of candidacidal and candidastatic activities of human neutrophils.

    abstract::Disruption of neutrophils causes the release of a cytoplasmic protein which can inhibit the growth of Candida albicans without killing the organisms. The present study was undertaken with a combined system in which candidacidal and candidastatic activities of human neutrophils could be compared. In this system it was ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sohnle PG,Collins-Lech C

    更新日期:1990-08-01 00:00:00

  • Influence of intranasal immunization with synthetic peptides corresponding to conserved epitopes of M protein on mucosal colonization by group A streptococci.

    abstract::A major virulence factor of group A streptococci is M protein, a surface-exposed fibrillar molecule of which there exist more than 80 distinct serological types. Antigenic variability resides largely in the amino-terminal region of M protein, whereas the carboxy-terminal half of the molecule is highly conserved among ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bessen D,Fischetti VA

    更新日期:1988-10-01 00:00:00

  • Use of mitochondrial and ribosomal DNA polymorphisms to classify clinical and soil isolates of Histoplasma capsulatum.

    abstract::We have developed an improved scheme for the classification of environmental and clinical isolates of Histoplasma capsulatum that is based on analysis of mitochondrial DNA (mtDNA) and ribosomal DNA (rDNA). Strains were initially divided into mtDNA groups according to restriction digests of whole-cell DNA and Southern ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Spitzer ED,Lasker BA,Travis SJ,Kobayashi GS,Medoff G

    更新日期:1989-05-01 00:00:00

  • Amyloidosis and the serum amyloid A protein response to muramyl dipeptide analogs and different mycobacterial species.

    abstract::Serum amyloid A protein (SAA) elevation accompanies induction of secondary amyloidosis in mice given Mycobacterium butyricum in Freund adjuvant. The synthesis of SAA by cultured hepatocytes is induced by a macrophage-derived mediator, which has been identified as interleukin 1. In these studies, SAA synthesis has been...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: McAdam KP,Foss NT,Garcia C,DeLellis R,Chedid L,Rees RJ,Wolff SM

    更新日期:1983-03-01 00:00:00

  • Native surface association of a recombinant 38-kilodalton Treponema pallidum antigen isolated from the Escherichia coli outer membrane.

    abstract::A recombinant plasmid designated pAW305, containing a 6-kilobase insert of Treponema pallidum DNA, directed the expression of a 38-kilodalton (kDa) treponemal antigen in Escherichia coli. The 38-kDa antigen copurified with the outer membrane fraction of the E. coli cell envelope after treatment with nonionic detergent...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fehniger TE,Radolf JD,Walfield AM,Cunningham TM,Miller JN,Lovett MA

    更新日期:1986-05-01 00:00:00

  • Different regions of the malaria merozoite surface protein 1 of Plasmodium chabaudi elicit distinct T-cell and antibody isotype responses.

    abstract::In this study we have investigated the antibody and CD4 T-cell responses to the well-characterized malaria vaccine candidate MSP-1 during the course of a primary Plasmodium chabaudi chabaudi (AS) infection. Specific antibody responses can be detected within the first week of infection, and CD4 T cells can be detected ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Quin SJ,Langhorne J

    更新日期:2001-04-01 00:00:00