Abstract:
:Henle 407 and HeLa cells were compared as hosts for Shigella dysenteriae at a low multiplicity of infection. Efficiency of attachment and invasion without centrifugation, as well as selectivity for pathogenic over nonpathogenic S. dysenteriae without Congo red, were much greater for Henle 407 cells than for HeLa cells.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Sen A,Leon MA,Palchaudhuri Sdoi
10.1128/IAI.58.7.2401-2403.1990subject
Has Abstractpub_date
1990-07-01 00:00:00pages
2401-3issue
7eissn
0019-9567issn
1098-5522journal_volume
58pub_type
杂志文章abstract::Surface proteins in gram-positive bacteria are frequently required for virulence, and many are attached to the cell wall by sortase enzymes. Bacteria frequently encode more than one sortase enzyme and an even larger number of potential sortase substrates that possess an LPXTG-type cell wall sorting signal. In order to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.2710-2722.2004
更新日期:2004-05-01 00:00:00
abstract::In this study, we examined the binding of Candida albicans synchronized yeast-phase cells to plastic, immobilized amino acids and bovine serum albumin (BSA) and quantified the binding by using an XTT tetrazolium salt assay and absorbance determination. Our results show that C. albicans binds efficiently and specifical...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.1.140-144.1998
更新日期:1998-01-01 00:00:00
abstract::Haemophilus ducreyi is resistant to killing by normal serum antibody and complement. We discovered an H. ducreyi outer membrane protein required for expression of serum resistance and termed it DsrA (for "ducreyi serum resistance A"). The dsrA locus was cloned, sequenced, and mutagenized. An isogenic mutant (FX517) of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1608-1619.2000
更新日期:2000-03-01 00:00:00
abstract::The mechanism by which Helicobacter pylori, which has little or no invasive activity, induces gastric-tissue inflammation and injury has not been well characterized. We have previously demonstrated that water-extracted proteins of H. pylori are capable of activating human monocytes by a lipopolysaccharide (LPS)-indepe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1183-1187.1995
更新日期:1995-04-01 00:00:00
abstract::Bordetella pertussis extract that contained adenylate cyclase toxin produced large increases in human neutrophil cyclic AMP levels and inhibited their oxidative burst, as reflected by luminol-enhanced chemiluminescence and superoxide release. The adenylate cyclase toxin-containing extract blocked neutrophil-mediated i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.751-755.1988
更新日期:1988-04-01 00:00:00
abstract::Exposure of hamster tracheal rings in organ culture to virulent Mycoplasma pneumoniae organisms leads to alterations in macromolecular biosynthesis and metabolic activity of the respiratory epithelial cells. Avirulent organisms derived from the same parent strain do not produce these effects. During the course of infe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.4.704-710.1975
更新日期:1975-04-01 00:00:00
abstract::We have studied the ability of outer membrane vesicle (OMV) vaccines from Neisseria meningitidis serogroup B to induce vaccine-specific antibody and spleen cell proliferative responses in mice after being administered intranasally (i.n.) and/or subcutaneously (s.c.). A series of four weekly i.n. doses (25 microg) with...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.5010-5015.2001
更新日期:2001-08-01 00:00:00
abstract::Recent evidence suggests that immune-mediated gastric epithelial cell apoptosis through Fas-Fas ligand interactions participates in Helicobacter pylori disease pathogenesis. To define the role of Fas signaling in vivo, H. pylori strain SS1 infection in C57BL/6 mice was compared to that in mice deficient in the Fas lig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2591-2597.2002
更新日期:2002-05-01 00:00:00
abstract::Chronic Helicobacter pylori infection is associated with mucosal inflammation. The aim of the present study was to assess human neutrophil and monocyte activation by H. pylori strains obtained from patients with different clinical presentations. Bacterial sonicates from 12 strains were used to stimulate phagocyte upre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.3171-3174.1999
更新日期:1999-06-01 00:00:00
abstract::Current efforts to develop plague vaccines focus on LcrV, a polypeptide that resides at the tip of type III secretion needles. LcrV-specific antibodies block Yersinia pestis type III injection of Yop effectors into host immune cells, thereby enabling phagocytes to kill the invading pathogen. Earlier work reported that...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05637-11
更新日期:2012-04-01 00:00:00
abstract::The extracellular adherence protein (Eap) is a multifunctional Staphylococcus aureus protein and broad-spectrum adhesin for several host matrix and plasma proteins. We investigated the interactions of full-length Eap and five recombinant tandem repeat domains with host proteins by use of surface plasmon resonance (BIA...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00480-08
更新日期:2008-12-01 00:00:00
abstract::Differences in susceptibility to persistent cryptosporidial infection between two strains of adult athymic nude mice prompted us to investigate the immune mechanism(s) that may control resistance to infection in these T-cell-deficient mice. We studied fecal oocyst shedding, serum and fecal parasite-specific antibody r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.3947-3951.1999
更新日期:1999-08-01 00:00:00
abstract::The mechanisms of immunity to salmonellae conferred by immunization with live vaccines were studied by adoptive transfer using the mouse-virulent strain Salmonella typhimurium C5 and innately susceptible BALB/c (ltys) mice. This organism cannot establish a sublethal infection in naive BALB/c mice. Animals immunized 2 ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3981-3984.1993
更新日期:1993-09-01 00:00:00
abstract::Resistance against acute and chronic infection with Taxoplasma gondii in BALB/c and CBA/Ca mice was compared. Intraperitoneal inoculation of either 20, 40, or 80 cysts of the ME49 strain resulted in mortality rates in BALB/c mice of 12% (2 of 17), 50% (6 of 12), and 75% (9 of 12), respectively, within 3 weeks after in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2284-2288.1993
更新日期:1993-06-01 00:00:00
abstract::Apical membrane antigen 1 (AMA-1) is an invasion-related Plasmodium antigen that is expressed during both intracellular and extracellular asexual stages of the parasite's life cycle, making it an ideal target for induction of humoral and cellular immune responses that can protect against malaria. We show here that whe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05048-11
更新日期:2011-09-01 00:00:00
abstract::Several immunological, hematological, and pathological responses associated with Marek's disease were determined. Four-week-old Marek's disease-infected and control chickens were injected with Salmonella pullorum antigen. About one-half of all infected chickens tested were unresponsive to antigenic challenge. Antibody...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.5.567-574.1971
更新日期:1971-11-01 00:00:00
abstract::We measured the microbicidal peptides MCP-1 and MCP-2 in rabbit alveolar macrophages (AM), comparing rabbits pretreated with complete Freund adjuvant with untreated control animals. Levels of MCP-1 increased from 4.7 +/- 0.6 microgram/10(7) resident AM to 13.2 +/- 0.1 microgram/10(7) complete Freund adjuvant-elicited ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.3.775-778.1981
更新日期:1981-09-01 00:00:00
abstract::Temperate bacteriophage can transfer toxin-encoding genes between bacteria, often resulting in acquired pathogenicity. However, little is known regarding the effects of the eukaryotic host on the phage-pathogen interaction. Using Streptococcus pyogenes as a model, we demonstrate, both in vitro and in vivo, that the eu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.3782-3786.2003
更新日期:2003-07-01 00:00:00
abstract::In this study, we report on a novel approach based on modification of the intestinal surface to prevent diarrhea caused by enterotoxigenic Escherichia coli (ETEC). The removable intestinal tie adult rabbit diarrhea (RITARD) model was used to test the efficacy of an enteric-coated protease preparation (Detach; Enzacor ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3708-3714.1991
更新日期:1991-10-01 00:00:00
abstract::Nasopharyngeal colonization represents the initial interaction between Haemophilus influenzae and its human host. Factors that influence bacterial carriage likely affect transmission and incidence of infection. Therefore, we investigated host factors involved in limiting H. influenzae colonization in BALB/c mice, as c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01564-08
更新日期:2009-08-01 00:00:00
abstract::The goal of this study is to evaluate the contribution of mast cells to Helicobacter pylori immunity in a model of vaccine-induced protection. Mast cell-deficient Kitl(Sl)/Kitl(Sl-d) and control mice were immunized with H. pylori sonicate plus cholera toxin and challenged with H. pylori, and the bacterial loads, infla...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00532-09
更新日期:2009-12-01 00:00:00
abstract::The mechanisms of phagocytosis of Candida albicans by human vascular endothelial cells and subsequent endothelial cell injury were examined in vitro. Both live and killed C. albicans cells were phagocytized by endothelial cells. This organism specifically induced endothelial cell phagocytosis because neither Candida t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.976-983.1995
更新日期:1995-03-01 00:00:00
abstract::Genetic resistance to lethal infection with Rickettsia tsutsugamushi was studied in over 30 inbred strains, inbred hybrids, and outbred stocks of mice. Inbred mice infected intraperitoneally with the Gilliam strain of R. tsutsugamushi showed three patterns of response: susceptible (A/HeJ, C3H/HeDub, C3H/HeJ, C3H/HeN, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.2.583-588.1978
更新日期:1978-02-01 00:00:00
abstract::The immune response to the mycobacterial 65-kDa heat shock protein (hsp65) is considered an important event in the induction of adjuvant arthritis (AA) in rats; this induction probably occurs through a molecular mimicry mechanism involving cross-reactivity against the rat homolog hsp60. To analyze the role of mammalia...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4225-4231.1993
更新日期:1993-10-01 00:00:00
abstract::The properties of two candidate Salmonella typhi-based live oral typhoid vaccine strains, BRD691 (S. typhi Ty2 harboring mutations in aroA and aroC) and BRD1116 (S. typhi Ty2 harboring mutations in aroA, aroC, and htrA), were compared in a number of in vitro and in vivo assays. BRD1116 exhibited an increased susceptib...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.700-707.1999
更新日期:1999-02-01 00:00:00
abstract::Neisseria meningitidis is the causative agent of meningococcal disease. Onset of meningococcal disease can be extremely rapid and can kill within a matter of hours. However, although a much-feared pathogen, Neisseria meningitidis is frequently found in the nasopharyngeal mucosae of healthy carriers. The bacterial fact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02001-14
更新日期:2014-12-01 00:00:00
abstract::Susceptibility of Syrian hamsters of the inbred LSH and MHA strains to injection of as few as 10 plaque-forming units of vesicular stomatitis virus (VSV) was shown to occur only after intraperitoneal and intrapleural injection and not after injection of VSV intravenously, intranasally, or in the footpads. Despite the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.540-549.1981
更新日期:1981-11-01 00:00:00
abstract::NF-κB is a critical regulator of gene expression during severe infections. NF-κB comprises homo- and heterodimers of proteins from the Rel family. Among them, p50 and p65 have been clearly implicated in the pathophysiology of sepsis. In contrast, the role of cRel in sepsis is still controversial and has been poorly st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00021-11
更新日期:2011-05-01 00:00:00
abstract::Relapsing fever (RF) is a spirochetal infection characterized by relapses of a febrile illness and spirochetemia due to the sequential appearance and disappearance of isogenic serotypes in the blood. The only difference between isogenic serotypes is the variable major outer membrane lipoprotein. In the absence of spec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00538-06
更新日期:2006-11-01 00:00:00
abstract::Previous studies have shown that a mutant (designated C4) of Streptococcus mutans 6715 wild type (WT) is defective in glucosyltransferase (GTF)-synthesized insoluble glucan and is avirulent in gnotobiotic rats. This study investigated the factors which would render this mutant virulent in gnotobiotic rats. Microbial a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.3.915-921.1980
更新日期:1980-03-01 00:00:00