Abstract:
:Relapsing fever (RF) is a spirochetal infection characterized by relapses of a febrile illness and spirochetemia due to the sequential appearance and disappearance of isogenic serotypes in the blood. The only difference between isogenic serotypes is the variable major outer membrane lipoprotein. In the absence of specific antibody, established serotypes cause persistent infection. Studies in our laboratory indicate that another consequence of serotype switching in RF is a change in neuroinvasiveness. As the next step to elucidate this phenomenon, we studied the interaction of the neurotropic Oz1 strain of the RF agent Borrelia turicatae with the cerebral microcirculation. During persistent infection of antibody-deficient mice, we found that serotype 1 entered the brain in larger numbers and caused more severe cerebral microgliosis than isogenic serotype 2. Microscopic examination revealed binding of B. turicatae to brain microvascular endothelial cells in vivo. In vitro we found that B. turicatae associated with brain microvascular endothelial cells (BMEC) significantly more than with fibroblasts or arachnoidal cells. The binding was completely eliminated by pretreatment of BMEC with proteinase K. Using transwell chambers with BMEC barriers, we found that serotype 1 crossed into the lower compartment significantly better than serotype 2. Heat killing significantly reduced BMEC crossing but not binding. We concluded that the interaction of B. turicatae with the cerebral microcirculation involves both binding and crossing brain microvascular endothelial cells, with significant differences among isogenic serotypes.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Sethi N,Sondey M,Bai Y,Kim KS,Cadavid Ddoi
10.1128/IAI.00538-06subject
Has Abstractpub_date
2006-11-01 00:00:00pages
6408-18issue
11eissn
0019-9567issn
1098-5522pii
IAI.00538-06journal_volume
74pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.56.1.13-17.1988
更新日期:1988-01-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1992-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Infection and immunity
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更新日期:2005-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2016-04-22 00:00:00
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pub_type: 杂志文章
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更新日期:2002-06-01 00:00:00