Abstract:
:In this study, we examined the binding of Candida albicans synchronized yeast-phase cells to plastic, immobilized amino acids and bovine serum albumin (BSA) and quantified the binding by using an XTT tetrazolium salt assay and absorbance determination. Our results show that C. albicans binds efficiently and specifically to several nonpolar aliphatic amino acids and positively charged amino acids and to BSA immobilized on tissue culture plastic but not to polar uncharged, negatively charged, or aromatic amino acids. Adhesion of yeasts to immobilized amino acids was not affected by preincubation of cells with BSA, whereas binding to immobilized BSA was affected by preincubation of yeasts with alanine, proline, and leucine but not by arginine or lysine. The ability to distinguish the chirality of these amino acids was also examined by using both the D and L amino acid configurations, and the results show that C. albicans yeasts recognize only the L configuration of these amino acids. The observations that C. albicans specifically binds to certain amino acids indicate that these amino acids may prove useful tools for studying the binding interactions of C. albicans yeasts with host proteins such as components of the extracellular matrix.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Hawser SP,Islam Kdoi
10.1128/IAI.66.1.140-144.1998subject
Has Abstractpub_date
1998-01-01 00:00:00pages
140-4issue
1eissn
0019-9567issn
1098-5522journal_volume
66pub_type
杂志文章abstract::In order to develop a new system for the analysis of capsular biosynthetic pathways we have explored the possibility of expressing type 3 capsular polysaccharide (CPS) from the pathogen Streptococcus pneumoniae in Lactococcus lactis, an unencapsulated lactic acid bacterium being developed as a vaccine delivery vehicle...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3251-3260.2000
更新日期:2000-06-01 00:00:00
abstract::The bactericidal and opsonizing effects of normal human serum on six strains of Salmonella typhimurium LT-2 having different lipopolysaccharide (LPS) composition were demonstrated through five indices. Complement activity in the presence of antibody was important for the opsonization of all six strains and for the bac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.647-653.1985
更新日期:1985-09-01 00:00:00
abstract::Lyme disease (LD), the most prevalent tick-borne illness in North America, is caused by Borrelia burgdorferi The long-term survival of B. burgdorferi spirochetes in the mammalian host is achieved though VlsE-mediated antigenic variation. It is mathematically predicted that a highly variable surface antigen prolongs ba...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00890-16
更新日期:2016-12-29 00:00:00
abstract::Growth of a streptomycin-resistant strain of Escherichia coli (VL-2) in the presence of 30 microgram of streptomycin per ml resulted in the production by these bacteria of structurally altered, nonfunctional type 1 fimbriae. This strain, when grown in this subinhibitory concentration of streptomycin, became incapable ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.792-797.1981
更新日期:1981-02-01 00:00:00
abstract::Cellular resistance to facultative intracellular parasites has been studied by determining the antimycobacterial activity and the amount of fatty acids in sera and in heptane extracts of freshly collected and 24-h-cultured normal and activated guinea pig alveolar macrophages and liver cells. The quantity and the antim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.1.170-177.1978
更新日期:1978-01-01 00:00:00
abstract::The adhesion of gram-positive bacteria to extracellular matrix (ECM) proteins is regarded as an important determinant of pathogenicity. A comparison of the adhesion of Streptococcus agalactiae strain O90R to different ECM proteins showed that the most pronounced binding could be observed for immobilized fibrinogen. To...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2434-2440.2002
更新日期:2002-05-01 00:00:00
abstract::Streptococcus pneumoniae infections of the lung are associated with significant damage to the alveolar epithelium. Host phagocytes and pneumolysin, a cytolytic toxin of S. pneumoniae, are believed to contribute to this cellular damage, yet experiments in which these elements are absent demonstrate the presence of an a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4392-4397.1993
更新日期:1993-10-01 00:00:00
abstract::Yersinia pestis, the agent of plague, has arisen from a less virulent pathogen, Yersinia pseudotuberculosis, by a rapid evolutionary process. Although Y. pestis displays a large number of virulence phenotypes, it is not yet clear which of these phenotypes descended from Y. pseudotuberculosis and which were acquired in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.10.5892-5899.2003
更新日期:2003-10-01 00:00:00
abstract::As an intracellular protozoan parasite, Toxoplasma gondii is likely to exploit proteases for host cell invasion, acquisition of nutrients, avoidance of host protective responses, escape from the parasitophorous vacuole, differentiation, and other activities. T. gondii serine protease inhibitor 1 (TgPI1) is the most ab...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06167-11
更新日期:2012-03-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) is a common cause of otitis media and respiratory tract infections. Outer membrane proteins (OMP) and lipooligosaccharide (LOS) are major surface antigens of NTHi and potential vaccine candidates. De-O-acylated LOS (dLOS) or oligosaccharide (OS) was coupled to total OMP to for...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5508-5513.1999
更新日期:1999-10-01 00:00:00
abstract::Toxin A (TcdA) and toxin B (TcdB) are major virulence factors of Clostridium difficile. These two toxins intoxicate cultured cells by similar mechanisms, and TcdB generally is more potent than TcdA in cultured cells. The exact reason for this difference is unclear. Here, we report that the cellular effects of TcdA can...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01577-08
更新日期:2009-06-01 00:00:00
abstract::A recent study demonstrated that polyclonal antibodies to Rickettsia conorii and monoclonal antibodies to outer membrane proteins A (OmpA) and B (OmpB) provided effective, Fc-dependent, passive immunity, even in severe combined immunodeficient mice with an established infection. In order to determine the mechanism of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.6.3524-3530.2004
更新日期:2004-06-01 00:00:00
abstract::Bacterial infection with group B Streptococcus (GBS) represents a prominent threat to neonates and fetuses in the Western world, causing severe organ damage and even death. To improve current therapeutic strategies and to investigate new approaches, an appropriate in vivo model to study the immune response of a human ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01235-12
更新日期:2013-05-01 00:00:00
abstract::Herein we characterized various genetic markers and the biological behavior of a natural recombinant strain of Toxoplasma gondii (P-Br). From nine genetic markers analyzed, three (B1, ROP1, and SAG1) and three (cS10-A6, GRA6, and SAG3) markers belong to parasites from the type I and type III lineages, respectively. Th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6392-6401.2003
更新日期:2003-11-01 00:00:00
abstract::A solid-phase radioimmunoassay method has been developed for the detection of rotavirus in the form of a purified antigen and in stool. The parameters of the radioimmunoassay were examined and optimized to give high sensitivity and same-day results. Compared with electron microscopy, the assay is up to 10 times as sen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.439-444.1977
更新日期:1977-05-01 00:00:00
abstract::Surface proteins in gram-positive bacteria are frequently required for virulence, and many are attached to the cell wall by sortase enzymes. Bacteria frequently encode more than one sortase enzyme and an even larger number of potential sortase substrates that possess an LPXTG-type cell wall sorting signal. In order to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.2710-2722.2004
更新日期:2004-05-01 00:00:00
abstract::A large body of evidence has convincingly shown that Toll-like receptors are necessary sensors for infections with pathogens, but their activation was also suggested to generate autoimmunity. During experimental infections, the lack of these sensors or of their signaling molecules should lead to a deficient immune res...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00951-07
更新日期:2008-04-01 00:00:00
abstract::Gender is a significant factor in determining the susceptibility to and severity of pulmonary diseases in both humans and animals. Murine respiratory mycoplasmosis (MRM), due to Mycoplasma pulmonis infection, is an excellent animal model for evaluation of the role of various host factors on the development of acute or...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.2865-2871.2001
更新日期:2001-05-01 00:00:00
abstract::Infection with the protozoan parasite Toxoplasma gondii is transmitted to humans from infected animals by tissue cysts and oocysts excreted by cats. Immunization with inactivated parasites or recombinant proteins has at best shown partial protection. We constructed a plasmid expressing the SAG1 surface antigen of T. g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::The hemolysins of Serpulina hyodysenteriae are active at 27 to 40 degrees C and pH 3 to 9 and are unaffected by enzymatic inhibitors. Pore formation was demonstrated by the inhibition of hemolysis with molecules of 2.0 to 2.3 nm in diameter and the release of 86rubidium from erythrocytes without hemoglobin release aft...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.11.4877-4879.1997
更新日期:1997-11-01 00:00:00
abstract::Recruitment of neutrophils to lung tissue and airspaces is a hallmark of inflammatory events following inhalation of endotoxins. We studied the role of different lymphocyte subsets in this inflammation, which is assumed to primarily involve the innate immune system. Inhalation of aerosolized Escherichia coli lipopolys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6962-6969.2000
更新日期:2000-12-01 00:00:00
abstract::This study compared infection and transformation of peripheral blood mononuclear cells (PBM) of Boran cattle and African buffalo in vitro to determine whether differences occurred which could account for the greater susceptibility of Boran cattle to infection with Theileria parva subsp. parva and T. parva subsp. lawre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.1.186-191.1986
更新日期:1986-07-01 00:00:00
abstract::A GroEL-like protein from Bordetella pertussis was purified. This protein was found to have the tetradecameric subunit structure typical of the GroEL family of proteins and to contain epitopes similar to those of other members of this family, including a human GroEL-like protein. Active immunization of neonatal mice w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1417-1422.1991
更新日期:1991-04-01 00:00:00
abstract::Protective immunity to Plasmodium falciparum malaria acquired after natural exposure is largely antibody mediated. IgG-specific P. falciparum EMP1 (PfEMP1) proteins on the infected erythrocyte surface are particularly important. The transient antibody responses and the slowly acquired protective immunity probably refl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01514-13
更新日期:2014-05-01 00:00:00
abstract::Complement-fixing antibodies were first detected in mice 7 days after intravenous injection with Mycoplasma arthritidis. Peak titers were observed at 21 days, and high levels of antibody persisted through 293 days. The metabolism-inhibiting antibody response was minimal. On fractionation of mouse sera, only 7S antibod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.4.431-440.1971
更新日期:1971-10-01 00:00:00
abstract::We have previously shown that malaria parasites liberate exoantigens which, through a phospholipid component, stimulate mouse macrophages to secrete tumor necrosis factor (TNF), which are toxic to D-galactosamine-sensitized mice, and which therefore might be involved in pathology. Plasmodium yoelii exoantigens detoxif...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.5.1894-1901.1992
更新日期:1992-05-01 00:00:00
abstract::The protein P2 comprises a large proportion of the outer membrane of nontypable Haemophilus influenzae and functions as a porin. In view of the importance of the protein as a surface antigen, the present study was designed to purify and analyze P2 with particular emphasis on detection of antigenic determinants express...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1084-1089.1988
更新日期:1988-05-01 00:00:00
abstract::Temperate bacteriophage can transfer toxin-encoding genes between bacteria, often resulting in acquired pathogenicity. However, little is known regarding the effects of the eukaryotic host on the phage-pathogen interaction. Using Streptococcus pyogenes as a model, we demonstrate, both in vitro and in vivo, that the eu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.3782-3786.2003
更新日期:2003-07-01 00:00:00
abstract::Phase 1 and 2 clinical trials of group B streptococcal (GBS) capsular polysaccharide (CPS)-protein conjugate vaccines in healthy adults have demonstrated their safety and improved immunogenicity compared with uncoupled CPSs. Two recent trials sought to determine (i) whether adsorption of conjugate vaccine to aluminum ...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.69.11.6696-6701.2001
更新日期:2001-11-01 00:00:00
abstract::Lactoperoxidase catalyzes the oxidation of thiocyanate by hydrogen peroxide into hypothiocyanite, a reaction which can protect bacterial and mammalian cells from killing by hydrogen peroxide. The present study demonstrates, however, that lactoperoxidase in the presence of thiocyanate can actually potentiate the bacter...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.3.581-586.1984
更新日期:1984-06-01 00:00:00