Abstract:
:The immune response to the mycobacterial 65-kDa heat shock protein (hsp65) is considered an important event in the induction of adjuvant arthritis (AA) in rats; this induction probably occurs through a molecular mimicry mechanism involving cross-reactivity against the rat homolog hsp60. To analyze the role of mammalian molecule hsp60 in arthritis, we generated a recombinant vaccinia virus (hsp60-VV) carrying the human hsp60 gene inserted into the thymidine kinase locus under the control of the 7.5k vaccinia virus promoter. Human hsp60 is almost identical to its rat homolog (97.4% linear amino acid homology) and shares about 50% of amino acid positions with Mycobacterium tuberculosis hsp65. The latter supposedly carries a critical epitope for AA induction that is not present in human hsp60. Infections with hsp60-VV of monkey cell cultures led to the expression of the human hsp60 molecule, as evidenced by immunoblotting analysis with specific monoclonal antibodies. Also, Lewis rats infected with hsp60-VV produced specific antibodies, demonstrating the in vivo expression of human hsp60 in the infected animals. Therefore, we used hsp60-VV to analyze whether the delivery of hsp60 could affect the induction of AA in Lewis rats. hsp60-VV clearly reduced and retarded arthritic symptoms when administered to rats at day 7 after AA induction. In contrast, inoculation of rats with a control recombinant vaccinia virus did not affect the course of the disease. The improvement in AA with hsp60-VV administration was associated with a specific immune response, as determined by the presence of antibodies to hsp60 in the sera and the proliferation induced by hsp60 of T cells from popliteal lymph nodes. These results support a critical role for immunity to heat shock proteins in AA. Since the protective construct is virtually identical to rat homolog hsp60, we conclude that immunity directed to conserved areas of this family of proteins is directly involved in the pathogenesis of AA.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
López-Guerrero JA,López-Bote JP,Ortiz MA,Gupta RS,Páez E,Bernabeu Cdoi
10.1128/IAI.61.10.4225-4231.1993subject
Has Abstractpub_date
1993-10-01 00:00:00pages
4225-31issue
10eissn
0019-9567issn
1098-5522journal_volume
61pub_type
杂志文章abstract::Vibrio cholerae accessory colonization factor genes (acfA, B, C, and D) are required for efficient intestinal colonization. Expression of acf genes is under the control of a regulatory cascade that also directs the synthesis of cholera toxin and proteins involved in the biogenesis of the toxin-coregulated pilus. The g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3289-3298.1994
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.60-63.1982
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.3.1380-1382.1983
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.1.399-409.1983
更新日期:1983-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1047-1053.1984
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.400-404.2001
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.1.269-271.1990
更新日期:1990-01-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1766-1770.1988
更新日期:1988-07-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1993-08-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:2013-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1999-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1812-1816.1990
更新日期:1990-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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