Abstract:
:Helicobacter pylori infection of the stomach causes an active immune response that includes stimulation of inducible nitric oxide (NO) synthase (iNOS) expression. Although NO can kill H. pylori, the bacterium persists indefinitely, suggesting that NO production is inadequate. We determined if the NO derived from iNOS in macrophages was dependent on the availability of its substrate, L-arginine (L-Arg). Production of NO by H. pylori-stimulated RAW 264.7 cells was dependent on the L-Arg concentration in the culture medium, and the 50% effective dose for L-Arg was 220 microM, which is above reported plasma L-Arg levels. While iNOS mRNA induction was L-Arg independent, iNOS protein increased in an L-Arg-dependent manner that did not involve changes in iNOS protein degradation. L-lysine, an inhibitor of L-Arg uptake, attenuated H. pylori-stimulated iNOS protein expression, translation, NO levels, and killing of H. pylori. While L-Arg starvation suppressed global protein translation, at concentrations of L-Arg at which iNOS protein was only minimally expressed in response to H. pylori, global translation was fully restored and eukaryotic translation initiation factor alpha was dephosphorylated. H. pylori lacking the gene rocF, which codes for a bacterial arginase, induced higher levels of NO production by increasing iNOS protein levels. When murine gastric macrophages were activated with H. pylori, supraphysiologic levels of L-Arg were required to permit iNOS protein expression and NO production. These findings indicate that L-Arg is rate limiting for iNOS translation and suggest that the levels of L-Arg that occur in vivo do not permit sufficient NO generation by the host to kill H. pylori.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Chaturvedi R,Asim M,Lewis ND,Algood HM,Cover TL,Kim PY,Wilson KTdoi
10.1128/IAI.00578-07subject
Has Abstractpub_date
2007-09-01 00:00:00pages
4305-15issue
9eissn
0019-9567issn
1098-5522pii
IAI.00578-07journal_volume
75pub_type
杂志文章abstract::Chlamydial inclusions were demonstrated by indirect immunofluorescence (IF) with antiserum to the chlamydial group antigen when McCoy cell monolayers infected with either Chlamydia trachomatis or Chlamydia psittaci were fixed in formaldehyde or paraformaldehyde, provided the monolayer was not allowed to dry. If these ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.561-570.1981
更新日期:1981-11-01 00:00:00
abstract::Encapsulated cells of Cryptococcus neoformans are potent activators of the alternative complement pathway. Previous studies found that monoclonal antibodies (MAbs) specific for the major capsular polysaccharide, termed glucuronoxylomannan (GXM), can markedly suppress the ability of the capsule to accumulate C3 from no...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1547-1553.1998
更新日期:1998-04-01 00:00:00
abstract::Enterococcus faecalis strains are resident intestinal bacteria associated with invasive infections, inflammatory bowel diseases, and colon cancer. Although factors promoting E. faecalis colonization of intestines are not fully known, one implicated pathway is a phosphotransferase system (PTS) in E. faecalis strain OG1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00080-19
更新日期:2019-06-20 00:00:00
abstract::CS31A is a K88-related capsule-like surface protein that mediates Escherichia coli and Klebsiella pneumoniae adhesion to the human Caco-2 and Intestine-407 cell lines. In this study, we demonstrate that ClpG, the major subunit of CS31A, contains the adhesive domain of the polymerized structure. We mapped this domain w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.609-616.1997
更新日期:1997-02-01 00:00:00
abstract::A major goal of current vaccine development is the induction of strong immune responses against protective antigens delivered by mucosal routes. One of the most promising approaches in that respect relies on the use of live recombinant vaccine carriers. In this study, Mycobacterium bovis BCG was engineered to produce ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5669-5676.1998
更新日期:1998-12-01 00:00:00
abstract::Chromatin modification triggered by bacteria is a newly described mechanism by which pathogens impact host transcription. Listeria monocytogenes dephosphorylates histone H3 through the action of listeriolysin O (LLO); however, the underlying mechanism is unknown. Here we show that an unrelated pore-forming toxin, Aero...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01243-10
更新日期:2011-07-01 00:00:00
abstract::Clostridium botulinum neurotoxins (BoNTs) are the most toxic proteins for humans. The current clostridial-derived vaccines against BoNT intoxication have limitations including production and accessibility. Conditions were established to express the soluble receptor binding domain (heavy-chain receptor [HCR]) of BoNT s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6998-7005.2005
更新日期:2005-10-01 00:00:00
abstract::Host-adapted Gram-negative bacterial pathogens from the Pasteurellaceae, Neisseriaceae, and Moraxellaceae families normally reside in the upper respiratory or genitourinary tracts of their hosts and rely on utilizing iron from host transferrin (Tf) for growth and survival. The surface receptor proteins that mediate th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02572-14
更新日期:2015-03-01 00:00:00
abstract::A group of insertion mutants was used to define the genes of plasmid pDC5 required for the expression of mannose-resistant fimbriae. Minicell experiments identified four polypeptides (71,000, 45,000, 27,000, and 17,000 daltons) concerned with fimbrial production, the smallest of these being the fimbrial subunit. The a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.900-906.1983
更新日期:1983-12-01 00:00:00
abstract::Genomic DNA fingerprint analysis was performed on 39 Staphylococcus aureus and 28 Enterococcus faecalis endophthalmitis isolates collected from multiple clinical centers. Among 21 S. aureus genomic DNA fingerprint patterns identified, five clonotypes were recovered from multiple unrelated patients and accounted for 58...
journal_title:Infection and immunity
pub_type: 杂志文章,多中心研究
doi:10.1128/IAI.66.1.356-360.1998
更新日期:1998-01-01 00:00:00
abstract::The absolute requirement for elemental iron and the porphyrin nucleus for growth of Haemophilus influenzae led us to investigate the role of iron and hemin in regulation of expression of the H. influenzae transferrin receptor. H. influenzae type b strain H1689 was grown in brain heart infusion broth supplemented with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4033-4037.1993
更新日期:1993-10-01 00:00:00
abstract::We have identified a Crp/Fnr-like transcriptional regulator of Streptococcus pyogenes that when inactivated attenuates virulence. The gene, named srv for streptococcal regulator of virulence, encodes a 240-amino-acid protein with 53% amino acid similarity to PrfA, a transcriptional activator of virulence in Listeria m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.3.1799-1803.2004
更新日期:2004-03-01 00:00:00
abstract::Protection by surface antigen TF1.17 of Tritrichomonas foetus was investigated because it reacted with a monoclonal antibody which immobilized and mediated complement killing of the organism and prevented adherence to vaginal epithelial cells. This monoclonal antibody was used to demonstrate conservation of the antige...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.4.1385-1394.1993
更新日期:1993-04-01 00:00:00
abstract::Numerous investigations have shown that 70-kDa heat shock protein (Hsp70) homologs interact tightly with hydrophobic proteins and functionally assist proteins in membranous organelles and environments. One such protein is the Chlamydia trachomatis Hsp70 that is associated with isolated outer membrane complexes of infe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.535-543.2002
更新日期:2002-02-01 00:00:00
abstract::Francisella tularensis is a gram-negative pathogen that causes life-threatening infections in humans and has potential for use as a biological weapon. The genetic basis of the F. tularensis virulence is poorly understood. This study screened a total of 3,936 transposon mutants of the live vaccine strain for infection ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01865-06
更新日期:2007-06-01 00:00:00
abstract::Tuberculosis remains the leading cause of death among infectious diseases, accounting for more than two million deaths annually. The incidence of the disease is increasing globally, partially because of the resurgence of drug-resistant strains of Mycobacterium tuberculosis. Calixarenes are macrocyclic oligomers, some ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.11.6318-6323.2004
更新日期:2004-11-01 00:00:00
abstract::Salmonella choleraesuis chi 3781, an attenuated [delta cya-12 delta(crp-cdt)19] mutant, was electroporated with the plasmid pBA31-R7, which codes for the expression of a 31-kDa protein from Brucella abortus (BCSP31). Recombinant S. choleraesuis chi 3781 stably maintained the pBA31-R7 plasmid and continued to express t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.2.610-618.1993
更新日期:1993-02-01 00:00:00
abstract::Ehrlichia chaffeensis, a tick-transmitted rickettsial, is the causative agent of human monocytic ehrlichiosis. To examine protein expression patterns, we analyzed total, membrane, and immunogenic proteomes of E. chaffeensis originating from macrophage and tick cell cultures. Total proteins resolved by one-dimensional ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00484-08
更新日期:2008-11-01 00:00:00
abstract::To explore the mechanisms underlying the eosinophil-mediated inflammation of tropical pulmonary eosinophilia (TPE), bronchoalveolar lavage (BAL) fluid, serum, and supernatants from pulmonary and blood leukocytes (WBC) from patients with acute TPE (n = 6) were compared with those obtained from healthy uninfected indivi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1337-1342.2003
更新日期:2003-03-01 00:00:00
abstract::B-cell-deficient mice were prepared by administration of rabbit anti-mouse-mu antiserum to newborn animals within 12 h of birth onwards. Such immunodeficient animals, along with the normal controls, were infected intravenously with Cryptococcus neoformans. There was no difference in the mortality pattern, viable count...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.1-3.1979
更新日期:1979-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00956-07
更新日期:2008-02-01 00:00:00
abstract::Gamma interferon, an immune lymphokine that protects mouse macrophages against infection by several parasites, was ineffective against Mycobacterium lepraemurium. On the contrary, it significantly stimulated multiplication of M. lepraemurium in the macrophages. Simultaneous treatment of macrophages with gamma interfer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.8.2586-2587.1989
更新日期:1989-08-01 00:00:00
abstract::The ability of oral immunization to interfere with the establishment of infection with Helicobacter felis was examined. Groups of Swiss Webster mice were immunized orally with 250 micrograms of Helicobacter pylori recombinant urease (rUrease) and 10 micrograms of cholera toxin (CT) adjuvant, 1 mg of H. felis sonicate ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1246-1252.1995
更新日期:1995-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.1.41-47.1989
更新日期:1989-01-01 00:00:00
abstract::Vibrio cholerae is a monoflagellated gram-negative bacterium that causes the severe diarrheal disease cholera. In contrast to Salmonella enterica serovar Typhimurium infection, which is accompanied by both acute diarrhea and high-level inflammation, V. cholerae infection is largely noninflammatory in human hosts. Bact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00736-07
更新日期:2008-03-01 00:00:00
abstract::Cell-mediated immunity is critical in host resistance against the pathogenic fungus Histoplasma capsulatum. To explore the role of L3T4+ T cells in protection of mice against H. capsulatum infection, we examined the effect of in vivo treatment with anti-L3T4 monoclonal antibody (MAb) GK1.5 on the course of murine diss...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1685-1691.1988
更新日期:1988-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.11.6399-6408.2002
更新日期:2002-11-01 00:00:00
abstract::In vitro or animal models have been used to investigate the pathogenesis of Helicobacter pylori infection. However, extrapolation to humans of results obtained with these heterologous models remains difficult. We have developed a new model for the study of H. pylori infection that uses human entire embryonic stomachs ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::The antigenically variant Chlamydia trachomatis major outer membrane protein (MOMP) is a target of antibody-mediated neutralization in vitro, and it is an important protein for designing a subunit vaccine. Knowledge of MOMP T-cell determinants will be essential to elicit rapid and strong immune responses following an ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.9.3714-3718.1992
更新日期:1992-09-01 00:00:00
abstract::Conjugated polysaccharide vaccines protect against serogroup C meningococci. However, this approach cannot be applied to serogroup B, which is still a major cause of meningitis. We evaluated the immunogenicity of three surface-exposed proteins from serogroup B Neisseria meningitidis (App, NhhA, and NadA) identified du...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.4052-4060.2004
更新日期:2004-07-01 00:00:00