Abstract:
:To explore the mechanisms underlying the eosinophil-mediated inflammation of tropical pulmonary eosinophilia (TPE), bronchoalveolar lavage (BAL) fluid, serum, and supernatants from pulmonary and blood leukocytes (WBC) from patients with acute TPE (n = 6) were compared with those obtained from healthy uninfected individuals (n = 4) and from patients with asthma (n = 4) or elephantiasis (n = 5). Although there were no significant differences in the levels of interleukin-4 (IL-4), IL-5, IL-13, eotaxin, granulocyte-macrophage colony-stimulating factor, RANTES, or eosinophil cationic protein, there was a marked increase in eosinophil-derived neurotoxin (EDN) both systemically and in the lungs of individuals with TPE compared to each of the control groups (P < 0.02). Moreover, there was a compartmentalization of this response, with EDN levels being higher in the BAL fluid than in the serum (P < 0.02). Supernatants from WBC from either whole blood or BAL cells were examined for chemokines, cytokines, eosinophil degranulation products, and arachidonic acid metabolites. Of the many mediators examined-particularly those associated with eosinophil trafficking-only EDN (in BAL fluid and WBC) and MIP-1alpha (in WBC) levels were higher for TPE patients than for the non-TPE control groups (P < 0.02). These data suggest it is the eosinophilic granular protein EDN, an RNase capable of damaging the lung epithelium, that plays the most important role in the pathogenesis of TPE.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
O'Bryan L,Pinkston P,Kumaraswami V,Vijayan V,Yenokida G,Rosenberg HF,Crystal R,Ottesen EA,Nutman TBdoi
10.1128/iai.71.3.1337-1342.2003keywords:
subject
Has Abstractpub_date
2003-03-01 00:00:00pages
1337-42issue
3eissn
0019-9567issn
1098-5522journal_volume
71pub_type
杂志文章abstract::We have previously shown that a Pasteurella multocida cexA mutant (PBA875) was impaired in capsule export and highly attenuated in virulence for mice (J. D. Boyce and B. Adler, Infect. Immun. 68:3463-3468, 2000). In this study we show that immunization with high, but not low, doses of PBA875 can confer significant pro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1943-1946.2001
更新日期:2001-03-01 00:00:00
abstract::Previous studies in our laboratory have indicated that naturally resistant, inbred DBA/2J mice mount a greater serum antibody response to Pseudomonas aeruginosa 19660 than susceptible C57BL/6J mice. However, the specificity of the antibody produced was not known. The present study examines the specificity and kinetics...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.1984-1990.1991
更新日期:1991-06-01 00:00:00
abstract::Ribosomal subunits from Mycobacterium smegmatis were analyzed by using sedimentation velocity, sedimentation equilibrium, and acrylamide gel electrophoresis experiments. These s(20.w) values for the subunits are 48.7S and 28.1S. The molecular weight of the 49S subunit is about 1.65 x 10(6), and that of the 28S subunit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.3.489-496.1974
更新日期:1974-03-01 00:00:00
abstract::The method of hemagglutination inhibition was used to investigate the antigenic diversity of lipopolysaccharide (LPS) from Neisseria meningitidis and to develop a serotyping systems based on this antigen. The system uses outer membrane complex prepared by a simple extraction procedure to inhibit homologous hemagglutin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.471-475.1977
更新日期:1977-05-01 00:00:00
abstract::A gene library of Chlamydia trachomatis (serovar L1) DNA has been prepared in the phage vector lambda 1059. From this bank, 20 recombinant phage-expressing components which reacted with serum from a patient with a C. trachomatis (L1) infection were chosen. Selective expression and radiolabeling of phage polypeptides i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.637-641.1984
更新日期:1984-09-01 00:00:00
abstract::Deoxyribonucleic acid (DNA) base composition, as measured by guanine plus cytosine (G + C) content, was determined in 17 strains of Sporothrix schenckii and Ceratocystis species. The average G + C content for S. schenckii was 54.7 +/- 0.2 mol%. No strain of Ceratocystis, with the exception of C. minor, gave values for...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.5.934-938.1974
更新日期:1974-05-01 00:00:00
abstract::Among a number of laboratory strains of Listeria monocytogenes used in experimental infection, strain LO28 is highly capable of inducing robust beta interferon (IFN-β) production in infected macrophages. In this study, we investigated the molecular mechanism of the IFN-β-inducing ability of LO28 by comparing it with t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06162-11
更新日期:2012-07-01 00:00:00
abstract::Regulation of the synthesis of Vi polysaccharide, a major virulence determinant in Salmonella enterica serotype Typhi, is under the control of two regulatory systems, ompR-envZ and rscB-rscC, which respond to changes in osmolarity. Some serotype Typhi strains exhibit overexpression of Vi polysaccharide, which masks cl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00888-06
更新日期:2007-03-01 00:00:00
abstract::A nasal vaccine, consisting of outer membrane vesicles (OMVs) from group B Neisseria meningitidis, was given to 12 volunteers in the form of nose drops or nasal spray four times at weekly intervals, with a fifth dose 5 months later. Each nasal dose consisted of 250 microg of protein, equivalent to 10 times the intramu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1334-1341.1998
更新日期:1998-04-01 00:00:00
abstract::A toxin associated with Toxoplasma gondii infection was obtained from the trophozoites and culture medium used to propagate the parasite in cell cultures. The toxin, named Toxofactor (TF), administered parenterally or nonparenterally in adult mice, produces transient symptoms of lethargy, ruffled fur, and body weight ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1126-1135.1983
更新日期:1983-12-01 00:00:00
abstract::Actinobacillus actinomycetemcomitans SUNY 465, the invasion prototype strain, enters epithelial cells by an actin-dependent mechanism, escapes from the host cell vacuole, and spreads intracellularly and to adjacent epithelial cells via intercellular protrusions. Internalized organisms also egress from host cells into ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Peritoneal exudate cells from chickens immunized with two species of coccidia, Eimeria tenella or Eimeria maxima, were examined for their capacity to phagocytose stages of the parasite in vitro. True phagocytosis of the sporozoite stage is difficult to estimate because of its ability to invade cells, but may be evalua...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.4.862-871.1974
更新日期:1974-10-01 00:00:00
abstract::Legionella pneumophila was mutagenized with Tn903dIIlacZ, and a collection of mutants was screened for defects in macrophage killing (Mak-). Of 4,564 independently derived mutants, 55 (1.2%) showed a reduced or complete lack in the ability to kill HL-60-derived human macrophages. Forty-nine of the Mak- mutants could b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.12.5361-5373.1993
更新日期:1993-12-01 00:00:00
abstract::Adoptive immunity is poorly expressed in normal syngeneic mice. This phenomenon was studied by using experimental antituberculosis immunity as a model system representing pure cell-mediated immunity. Expression of adoptive immunity was facilitated by pretreating recipients with sublethal ionizing radiation (500 rads) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.1.257-263.1983
更新日期:1983-07-01 00:00:00
abstract::Altogether 171 serum specimens from 58 patients with heterophil antibody-positive infectious monomucleosis were studied for antibody response to Epstein-Barr virus (EBV). The sera were tested for fluorescent immunoglobulin G (IgG) and IgM gel-precipitating (GP) and complement-fixing (CF) antibodies to EBV. All 58 pati...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.42-51.1975
更新日期:1975-01-01 00:00:00
abstract::Candida albicans biofilms are formed through three distinct developmental phases and are associated with high fluconazole (FLU) resistance. In the present study, we used a set of isogenic Candida strains lacking one or more of the drug efflux pumps Cdr1p, Cdr2p, and Mdr1p to determine their role in FLU resistance of b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.8.4333-4340.2003
更新日期:2003-08-01 00:00:00
abstract::Wild-type isolates of Shigella flexneri bind the dye Congo red from solid media, thus producing red (Crb+) colonies. Mutants which fail to bind the dye produce white colonies (Crb-) and are avirulent in a variety of systems. In S. flexneri the ability to bind Congo red correlates with the ability to bind hemin and pro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1393-1398.1987
更新日期:1987-06-01 00:00:00
abstract::The dermotropism of Cryptococcus neoformans SLHA in congenitally athymic (nu/nu) and doubly immunodeficient beige-athymic (bg/bg-nu/nu) mice is described. Both bg/bg-nu/nu and nu/nu mice developed cutaneous cryptococcosis within 7 to 12 days following intravenous challenge with 10(4) encapsulated yeast cells. Macrosco...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.5.1785-1789.1991
更新日期:1991-05-01 00:00:00
abstract::Murine cerebral malaria (CM) induced by Plasmodium berghei ANKA kills susceptible mice within 24 to 48 h of onset of symptoms and is characterized by the production of inflammatory cytokines in the brain. C57BL/6J mice are sensitive to lethal CM, while A/J mice are resistant. These strains of mice were immunized with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5972-5979.1998
更新日期:1998-12-01 00:00:00
abstract::The comparative immunogenicity of the two cross-reacting group 6 pneumococcal capsular polysaccharides, type 6A(6) and type 6B(26), was studied with hyperimmune rabbit typing antisera and with sera from adult volunteers injected with polyvalent pneumococcal vaccines containing either 50 mug of type 6A (U.S. designatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1116-1122.1979
更新日期:1979-12-01 00:00:00
abstract::Five monoclonal antibodies (MAbs) were partially characterized and tested for their ability to inhibit penetration of Madin-Darby bovine kidney (MDBK) cells by sporozoites of Eimeria bovis. By indirect fluorescent-antibody assays, all MAbs reacted with acetone-fixed sporozoites, but only two MAbs, EbS9 (immunoglobulin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2538-2543.1988
更新日期:1988-10-01 00:00:00
abstract::Mannan (MAN) extracted from Candida albicans 20A was investigated for its potential as an antigen in the detection of cell-mediated immunity (CMI) in vivo and in vitro and for its ability to modulate CMI when administered intravenously (i.v.). CBA/J mice were either immunized as adults by the cutaneous inoculation of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.693-700.1989
更新日期:1989-03-01 00:00:00
abstract::The outer membrane of pathogenic Leptospira species grown in culture media contains lipopolysaccharide (LPS), a porin (OmpL1), and several lipoproteins, including LipL36 and LipL41. The purpose of this study was to characterize the expression and distribution of these outer membrane antigens during renal infection. Ha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.853-861.1999
更新日期:1999-02-01 00:00:00
abstract::We have previously shown that Bacillus anthracis lethal toxin represses glucocorticoid receptor (GR) transactivation. We now report that repression of GR activity also occurs with the large clostridial toxins produced by Clostridium sordellii and C. difficile. This was demonstrated using a transient transfection assay...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00291-07
更新日期:2007-08-01 00:00:00
abstract::A protein antigen called TR-o was isolated from supernatant of a sonically treated Reiter treponeme. The isolation procedure included anion-exchange chromatography on Whatman DE-52, hydrophobic interaction chromatography on decyl agarose, and finally gel filtration on Ac-A-22 Ultrogel. The fractionations were monitore...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.974-978.1982
更新日期:1982-03-01 00:00:00
abstract::Escherichia coli is the most common cause of urinary tract infection. Elevated blood and urine interleukin-6 (IL-6) levels have been shown in inflammatory urinary tract diseases. The role of IL-6 in mediating the urodynamic dysfunction in response to E. coli-induced urinary tract infection has not yet been fully eluci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00013-09
更新日期:2009-08-01 00:00:00
abstract::A battery of lymphoid and nonlymphoid cells was examined for susceptibility to lysis by natural killer cells of chickens. Several susceptible targets were recognized, and most susceptible among these were cells of line LSCC-RP9, derived from a lymphoid tumor induced by Rous-associated virus 2. The natural killer react...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.1078-1085.1981
更新日期:1981-03-01 00:00:00
abstract::A 46-kDa hemolytic protein, referred to as cystalysin, from Treponema denticola ATCC 35404 was overexpressed in Escherichia coli LC-67. Both the native and recombinant 46-kDa proteins were purified to homogeneity. Both proteins expressed identical biological and functional characteristics. In addition to its biologica...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.8.3231-3238.1997
更新日期:1997-08-01 00:00:00
abstract::Specific pathogen-free guinea pigs were infected via the respiratory route with viable, attenuated Mycobacterium tuberculosis H37Ra and maintained on purified isocaloric diets. The control diet contained 30% protein (ovalbumin) and 50 ppm of added zinc (50 micrograms/g), the low protein diet contained 10% protein and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.793-799.1983
更新日期:1983-02-01 00:00:00
abstract::It was previously determined that the type II secretion system (T2SS) promotes the ability of Legionella pneumophila to grow in coculture with amoebae. Here, we discerned the stage of intracellular infection that is potentiated by comparing the wild-type and T2SS mutant legionellae for their capacity to parasitize Aca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00374-19
更新日期:2019-10-18 00:00:00