Abstract:
:We have previously shown that a Pasteurella multocida cexA mutant (PBA875) was impaired in capsule export and highly attenuated in virulence for mice (J. D. Boyce and B. Adler, Infect. Immun. 68:3463-3468, 2000). In this study we show that immunization with high, but not low, doses of PBA875 can confer significant protection against wild-type challenge. We have also constructed a genetically defined acapsular P. multocida strain (AL18) by inactivation of bcbH, a gene predicted to be involved in polysaccharide biosynthesis. AL18 failed to produce immunoreactive polysaccharide as determined by immunofluorescence and Western immunoblot. Immunization of mice with live AL18 conferred significant protection against wild-type challenge, while immunization with similar doses of either killed wild-type or killed AL18 failed to confer protection.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Boyce JD,Adler Bdoi
10.1128/IAI.69.3.1943-1946.2001keywords:
subject
Has Abstractpub_date
2001-03-01 00:00:00pages
1943-6issue
3eissn
0019-9567issn
1098-5522journal_volume
69pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.2.480-485.1978
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1989-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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