Abstract:
:Ten micrograms of trehalose-6,6' -dimycolate (cord factor) injected into the footpads of mice increased the antibody response to sheep red blood cells (SRBC) subsequently injected into the same sites. There is a relationship between the antibody response and the cellular reaction induced locally and in the draining lymph nodes by cord factor, as judged by a much weaker response when antigen is injected into the contralateral footpads. The time intervals between injection of cord factor and antigen were from 5 to 20 days. A similar increased antibody response to SRBC was evident after preliminary administration of Freund's complete adjuvant or living BCG bacilli into the footpads. There was no increased antibody response in mice pretreated with living BCG or Freund's adjuvant to SRBC injected into the contralateral footpads. Administration of wax D from human strains Peurois and Test was without any effect. Administration of SRBC emulsified in incomplete Freund's adjuvant containing 5 mug of cord factor induced a very strong antibody response in the mice as compared to that after injection of the same amount of antigen in incomplete Freund's adjuvant containing wax D or mycobacteria. The results are discussed.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Bekierkunst A,Yarkoni E,Flechner I,Morecki S,Vilkas E,Lederer Edoi
10.1128/IAI.4.3.256-263.1971subject
Has Abstractpub_date
1971-09-01 00:00:00pages
256-63issue
3eissn
0019-9567issn
1098-5522journal_volume
4pub_type
杂志文章abstract::Chlamydia trachomatis L2 is an obligate intracellular microorganism with a unique biphasic life cycle. The extracellular form, the elementary body (EB), is infectious but metabolically inactive. Attachment of EBs to host cells is medicated by a heparan sulfate-like glycosaminoglycan. Following attachment, the EB is in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4900-4908.1994
更新日期:1994-11-01 00:00:00
abstract::Onchocerca volvulus, a human parasitic nematode, is the third leading cause of preventable blindness worldwide. This study describes the molecular cloning of a novel superoxide dismutase (SOD) from the parasite. This putative O. volvulus extracellular SOD (OvEcSOD) is 628 nucleotides (nt) long, including a 22-nt 5' sp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.713-716.1994
更新日期:1994-02-01 00:00:00
abstract::Alveolar bone destruction is a characteristic feature of periodontitis. Treponema denticola is known to be involved in periodontitis. To elucidate the role of T. denticola in alveolar bone destruction in periodontitis, the effects of lipooligosaccharide (LOS) from T. denticola on osteoclast formation and on expression...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.226-233.2003
更新日期:2003-01-01 00:00:00
abstract::Chlamydia trachomatis infections can lead to severe chronic complications, including trachoma, ectopic pregnancy, and infertility. The only effective approach to disease control is vaccination. The goal of this work was to identify new potential vaccine candidates through a proteomics approach. We constructed a protei...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00626-10
更新日期:2011-01-01 00:00:00
abstract::Immunization with the B oligomer of pertussis toxin protected neonatal mice from a lethal respiratory challenge with Bordetella pertussis. All mice immunized with 8 micrograms of B oligomer survived aerosol challenge and had peripheral leukocyte counts and weight gains similar to those of mice immunized with pertussis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.12.4063-4068.1990
更新日期:1990-12-01 00:00:00
abstract::An immune response (fluorescent antibody to membrane antigen) was detected in guinea pigs inoculated with varicella-zoster virus (VZV) adapted to guinea pig embryonic cells, including the Oka vaccine strain, even when inoculation was by an external route, i.e., nasal or corneal. Live or UV-inactivated virus having the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.2.407-412.1982
更新日期:1982-08-01 00:00:00
abstract::Mice immunized with infections of 500, 5,000, or 20,000 oocysts of E. falciformis var. pragensis were reinfected with 20,000 and 100,000 oocysts at 20 and 38 days, respectively, after the initial infection. After the first challenge infection, none of the immunized mice showed clinical signs of coccidiosis; a few mice...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.1.108-114.1979
更新日期:1979-01-01 00:00:00
abstract::It is proposed that binding of oral streptococci to saliva-coated hydroxylapatite (SHA) surfaces is a multifactorial process involving both specific and nonspecific receptors. In this context, specific binding is described as a high-affinity, saturable interaction between the cell and binding surface. Conversely, nons...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.3.653-659.1984
更新日期:1984-06-01 00:00:00
abstract::The survival of Staphylococcus aureus in the lungs of mice was studied under various conditions. Doses of 10(7) to 10(9) washed staphylococci were quantitatively introduced into the lungs after intratracheal inoculation in mice under either ether or sodium pentobarbital anesthesia. Mice were sacrificed at intervals, t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.1.114-123.1978
更新日期:1978-07-01 00:00:00
abstract::Help from CD4 T cells is often important for the establishment of primary and memory CD8 T-cell responses. However, it has yet to be determined whether T helper polarization affects the delivery of help and/or whether responding CD8 T cells helped by Th1 or Th2 cells express distinct effector properties. To address th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01328-06
更新日期:2007-05-01 00:00:00
abstract::Variation has been observed in the structural polypeptides of swine vesicular disease viruses isolated from the United Kingdom and Hong Kong. Despite the limited number of isolates examined, several distinct polypeptide patterns were obtained when the virus structural proteins were examined by polyacrylamide gel elect...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.593-599.1979
更新日期:1979-06-01 00:00:00
abstract::Cells infected by Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, display unusual intracellular morphological changes characterized by dilatation of the membranes of the endoplasmic reticulum and outer nuclear envelope. These changes are consistent with those that might be expected to occur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3110-3115.1988
更新日期:1988-12-01 00:00:00
abstract::No studies to date clearly define the interactions between Porphyromonas gingivalis and human peripheral blood polymorphonuclear leukocytes (PMN), nor has a protective role for antibody to P. gingivalis been defined. Using a fluorochrome phagocytosis microassay, we investigated PMN phagocytosis and killing of P. gingi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.2097-2104.1991
更新日期:1991-06-01 00:00:00
abstract::In its canonical role the reverse transcriptase telomerase recovers the telomeric repeats that are lost during DNA replication. Other locations and activities have been recently described for the telomerase protein subunit TERT in mammalian cells. In the present work, using biochemistry, molecular biology, and electro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02269-14
更新日期:2015-01-01 00:00:00
abstract::This work evaluated the kinetics of events that occur in the placenta of Calomys callosus after Toxoplasma gondii infection. Animals on the first day of pregnancy (dop) and virgin nonpregnant females were perorally infected with 20 cysts of T. gondii strain ME49. After 100 days of infection, the virgin animals were ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.7089-7094.2002
更新日期:2002-12-01 00:00:00
abstract::Stimulated polymorphonuclear leukocytes have been shown by this laboratory to release a reactive-oxygen species (ROS) which is detectable in the supernatant and is capable of oxidizing reduced glutathione and reacting with methionine (Sagone et al., Blood 63:96-104, 1984). This ROS is dependent on H2O2 and heme for it...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.846-849.1984
更新日期:1984-03-01 00:00:00
abstract::Chromosomes from Cryptococcus neoformans, an encapsulated yeast pathogen, were separated by contour-clamped homogeneous field gel electrophoresis. Seven strains representing all four serotypes were studied. It was found that each strain had a unique, reproducible pattern of chromosome bands which could potentially be ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.9.2624-2627.1989
更新日期:1989-09-01 00:00:00
abstract::Burkholderia cenocepacia, a member of the B. cepacia complex, is an opportunistic pathogen that causes serious infections in patients with cystic fibrosis. We identified a six-gene cluster in chromosome 1 encoding a two-component regulatory system (BCAL2831 and BCAL2830) and an HtrA protease (BCAL2829) hypothesized to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01581-06
更新日期:2007-04-01 00:00:00
abstract::The ability to bind Congo red (Crb+) is associated with virulence of Shigella flexneri and is encoded by a large, 220-kilobase plasmid. We cloned fragments of this plasmid to isolate the sequences encoding Congo red binding, to determine the degree of conservation of these sequences among S. flexneri strains, and to s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.435-443.1986
更新日期:1986-11-01 00:00:00
abstract::Vibrio cholerae responds to environmental changes by altering the protein composition of its outer membrane. In rich medium, V. cholerae expresses almost exclusively the outer membrane porin OmpU, whereas in minimal medium, OmpT is the dominant porin. The supplementation of a minimal medium with a mixture of asparagin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05851-11
更新日期:2012-02-01 00:00:00
abstract::A synthetic peptide whose sequence was derived from the circumsporozoite protein of Plasmodium knowlesi coupled to bovine gamma globulin has been shown to be immunogenic when administered with Freund complete adjuvant. The present experiments were designed to test the immunogenicity of the peptide when attached to a t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.839-842.1985
更新日期:1985-06-01 00:00:00
abstract::A panel of monoclonal antibodies (MAbs) specific for the heat-stable enterotoxin (STh) of enterotoxigenic Escherichia coli was produced. All four MAbs (8G7, 53-4, 11C, and SH1) bound to native STh in an enzyme-linked immunosorbent assay to various degrees, with clone SH1 showing the best affinity. The MAbs were screen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.289-294.1993
更新日期:1993-01-01 00:00:00
abstract::An immunogenic aminopeptidase was purified from Brucella melitensis strain VTRM1. The purification procedure consisted of ammonium sulfate fractionation and three chromatographic steps. This procedure resulted in a yield of 29% and a 144-fold increase in specific activity. The aminopeptidase appeared to be a monomeric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5238-5244.2003
更新日期:2003-09-01 00:00:00
abstract::Mycobacterium marinum grows at an optimal temperature of 33 degrees C, far lower than that for M. tuberculosis. Consequently, M. marinum infection of mammals is restricted largely to the cooler surfaces of the body, such as the extremities, but it causes a systemic infection in a large number of poikilothermic animals...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.767-773.1997
更新日期:1997-02-01 00:00:00
abstract::Here we describe a protocol for purifying Borrelia burgdorferi from feeding ticks by velocity centrifugation and Percoll density gradient centrifugation. The purified spirochetes were motile and 10- to 20-fold purer than the bacteria in crude tick homogenates. The purified bacteria were present in sufficient quantity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3536-3541.2001
更新日期:2001-06-01 00:00:00
abstract::Purified R-28 antigen from an M-protein-poor, R-antigen-rich strain of group A Streptococcus was prepared by sequential treatment of an acid extract of whole cells with ammonium sulfate fractionation and hydroxylapatite (HA) column chromatography. Purified R-28 antigen was eluted only with 0.10 M sodium phosphate, pH ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.4.901-909.1975
更新日期:1975-10-01 00:00:00
abstract::The attachment and invasion of chicken ovarian granulosa cells by Salmonella enteritidis was examined in vitro. The attachment was inhibited by preincubation of granulosa cells with anti-chicken fibronectin antibody (approximately 70% reduction in attachment) or preincubation with a 14-kDa fimbrial protein isolated fr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5015-5021.1996
更新日期:1996-12-01 00:00:00
abstract::Oxygen consumption by Treponema pallidum was observed to be glucose dependent. Treponemes consumed O2 to a low dissolved O2 concentration of 0.01 mumol of O2 per ml when respiration ceased. A fermentative degradation of glucose occurred during the maintenance of treponemes at 0.01 mumol of O2 per ml. Although the init...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.992-997.1981
更新日期:1981-03-01 00:00:00
abstract::Microbial survival in a host is usually dependent on the ability of a pathogen to undergo changes that promote escape from host defense mechanisms. The human-pathogenic fungus Cryptococcus neoformans undergoes phenotypic switching in vivo that promotes persistence in tissue. By microarray and real-time PCR analyses, t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01079-08
更新日期:2009-01-01 00:00:00
abstract::Escherichia coli is a major cause of enteric/diarrheal diseases, urinary tract infections, and sepsis. E. coli K1 is the leading gram-negative organism causing neonatal meningitis, but the microbial basis of E. coli K1 meningitis is incompletely understood. Here we employed comparative genomic hybridization to investi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.4.2196-2206.2006
更新日期:2006-04-01 00:00:00