Abstract:
:Obligate vacuolar pathogens produce proteins that localize to the host cell-derived membranes of the vacuoles in which they reside, yielding unique organelles that are optimally suited for pathogen survival. Anaplasma phagocytophilum is an obligate vacuolar bacterium that infects neutrophils and causes the emerging and potentially fatal disease human granulocytic anaplasmosis. Here we identified APH_1387 as the first A. phagocytophilum-derived protein that associates with the A. phagocytophilum-occupied vacuolar membrane (AVM). APH_1387, also referred to as P100, is a 61.4-kDa acidic protein that migrates with an apparent molecular weight of 115 kDa on SDS-PAGE gels. It carries 3 tandem direct repeats that comprise 58% of the protein. Each APH_1387 repeat carries a bilobed hydrophobic alpha-helix domain, which is a structural characteristic that is consistent with the structure of chlamydia-derived proteins that traverse inclusion membranes. APH_1387 is not detectable on the surfaces of A. phagocytophilum dense core organisms bound at the HL-60 cell surface, but abundant APH_1387 is detected on the surfaces of intravacuolar reticulate cell and dense core organisms. APH_1387 accumulates on the AVM throughout infection. It associates with the AVM in human HL-60, THP-1, and HMEC-1 cells and tick ISE6 cells. APH_1387 is expressed and localizes to the AVM in neutrophils recovered from A. phagocytophilum-infected mice. This paper presents the first direct evidence that A. phagocytophilum actively modifies its host cell-derived vacuole.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Huang B,Troese MJ,Ye S,Sims JT,Galloway NL,Borjesson DL,Carlyon JAdoi
10.1128/IAI.01418-09subject
Has Abstractpub_date
2010-05-01 00:00:00pages
1864-73issue
5eissn
0019-9567issn
1098-5522pii
IAI.01418-09journal_volume
78pub_type
杂志文章abstract::Leukosis viruses of seven subgroups were tested for oncogenicity in chickens susceptible to virus infection and to development of lymphoid leukosis (LL) tumors. All subgroup A viruses and the subgroup B virus tested produced a high incidence of LL and other related neoplasms. Viruses of subgroup C and RAV-61 of subgro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.2.423-428.1977
更新日期:1977-02-01 00:00:00
abstract::The role of the hypothalamus-pituitary-adrenal (HPA) axis in regulating the growth of Mycobacterium avium in Mycobacterium bovis BCG-resistant and -susceptible congenic mice was evaluated. Restraint was used to activate the HPA axis, which resulted in an increase in the level of corticosterone in the plasma. Activatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4793-4800.1993
更新日期:1993-11-01 00:00:00
abstract::The var gene-encoded Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family is responsible for antigenic variation and sequestration of infected erythrocytes during malaria. We have previously grouped the 60 PfEMP1 variants of P. falciparum clone 3D7 into groups A and B/A (category A) and groups B, B/C, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01187-06
更新日期:2006-12-01 00:00:00
abstract::The effect of indomethacin on the net intestinal accumulation of fluid induced by Escherichia coli heat-stable (ST) enterotoxin in the infant mouse model was examined. Indomethacin, when administered with ST enterotoxin, caused a striking decrease in net intestinal fluid accumulation. This inhibition of ST activity wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.1.143-147.1978
更新日期:1978-10-01 00:00:00
abstract::YopE is a virulence factor that is secreted into host cells infected by Yersinia species. The YopE C-terminal domain has GTPase-activating protein (GAP) activity. The YopE N-terminal domain contains an epitope that is an immunodominant CD8(+) T cell antigen during primary infection of C57BL/6 mice with Yersinia pseudo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01687-14
更新日期:2014-07-01 00:00:00
abstract::Peptidoglycan from a Staphylococcus epidermidis strain, isolated from a patient with septicemia, was preincubated with human serum. This mixture was then investigated for its potency to induce tumor necrosis factor (TNF) secretion by human blood monocytes. TNF was measured in the supernatants by using a bioassay and/o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3837-3843.1994
更新日期:1994-09-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTL) against herpes simplex virus (HSV) were induced in vitro from human peripheral blood lymphocytes by stimulation with HSV antigen. CTL generated by HSV type 1 (HSV-1) antigen stimulation killed not only HSV-1-infected target cells but also HSV type 2 (HSV-2)-infected target cells, though a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.190-197.1983
更新日期:1983-04-01 00:00:00
abstract::Murine cerebral malaria (CM) induced by Plasmodium berghei ANKA kills susceptible mice within 24 to 48 h of onset of symptoms and is characterized by the production of inflammatory cytokines in the brain. C57BL/6J mice are sensitive to lethal CM, while A/J mice are resistant. These strains of mice were immunized with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5972-5979.1998
更新日期:1998-12-01 00:00:00
abstract::We identified a T-cell determinant of the 35-kDa antigen of Mycobacterium leprae which is discriminatory against cross-sensitization by its closely related homologue in Mycobacterium avium. From synthetic peptides covering the entire sequence, those with the highest affinity and permissive binding to purified HLA-DR m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1501-1504.1999
更新日期:1999-03-01 00:00:00
abstract::By subcutaneous treatment with an aqueous solution of 6-O-stearoyl-N-acetylmuramyl-L-alanyl-D-isoglutamine [6-O-CH3-(CH2)16-CO-MurNAc-L-Ala-D-isoGln] [referred to here as L18-MDP(Ala)], an augmentation of the resistance of mice to Escherichia coli, Pseudomonas aeruginosa. Staphylococcus aureus, and Candida albicans in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.292-300.1982
更新日期:1982-07-01 00:00:00
abstract::Intimate attachment to the host cell leading to the formation of attaching and effacing (A/E) lesions is an essential feature of enterohemorrhagic Escherichia coli (EHEC) O157:H7 pathogenesis. In a related pathogen, enteropathogenic E. coli (EPEC), this activity is dependent upon translocation of the intimin receptor,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.5.2389-2398.1999
更新日期:1999-05-01 00:00:00
abstract::Susceptibility to the lethal toxicity of endotoxin in mice pretreated with emulsified trehalose-6,6'-dimycolate was enhanced by (i) increasing the oil concentration while keeping the Tween concentration constant; and (ii) decreasing the Tween concentration, while keeping the oil concentration constant. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.2.571-572.1979
更新日期:1979-05-01 00:00:00
abstract::Bacillus anthracis, the etiological agent of anthrax disease, is a proven weapon of bioterrorism. Currently, the only licensed vaccine against anthrax in the United States is AVA Biothrax, which, although efficacious, suffers from several limitations. This vaccine requires six injectable doses over 18 months to stimul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00828-08
更新日期:2009-04-01 00:00:00
abstract::Bordetella bronchiseptica is closely related to Bordetella pertussis, which produces respiratory disease primarily in mammals other than humans. However, its importance as a human pathogen is being increasingly recognized. Although a large amount of research on Bordetella has been generated regarding protein virulence...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.1791-1798.2002
更新日期:2002-04-01 00:00:00
abstract::Little data are available regarding L-form phagocyte interactions. Due to difficulty with more conventional methods, a serum-free technique of surface phagocytosis employing cellulose ester micropore filters, fluorochrome staining, and reflecting fluorescence microscopy was developed. Intracellular localization of L-f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.6.976-981.1972
更新日期:1972-06-01 00:00:00
abstract::Intra-abdominal polymicrobial infections cause significant morbidity and mortality. An experimental mouse model of Candida albicans-Staphylococcus aureus intra-abdominal infection (IAI) results in 100% mortality by 48 to 72 h postinoculation, while monomicrobial infections are avirulent. Mortality is associated with r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01059-15
更新日期:2015-10-19 00:00:00
abstract::Three types of caseinolytic proteases (Pase-A, Pase-B, and Pase-C) were isolated and purified from culture supernatants of Porphyromonas gingivalis 381 by the combined procedures of acetone precipitation, gel filtration, solubilization with octylthioglucoside followed by affinity chromatography on arginine-Sepharose 4...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.3060-3068.1991
更新日期:1991-09-01 00:00:00
abstract::Streptococcus agalactiae (group B streptococcus [GBS]) is the leading cause of neonatal pneumonia, sepsis, and meningitis. An in silico genome analysis indicated that GBS strain NEM316 encodes 35 proteins containing an LPXTG motif which are thought to be covalently linked to the peptidoglycan by an enzyme called sorta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3342-3350.2005
更新日期:2005-06-01 00:00:00
abstract::Group A streptococcal pyrogenic exotoxins A, B, and C (also known as scarlet fever toxins and erythrogenic toxins) were evaluated for relatedness to another streptococcus-derived lymphocyte mitogen, blastogen A. Streptococcal pyrogenic exotoxin A and blastogen A were immunologically cross-reactive and shared the same ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1865-1867.1989
更新日期:1989-06-01 00:00:00
abstract::To study the immunological activity of proteins secreted by Mycobacterium tuberculosis, we carried out comparative studies in guinea pigs infected intravenously with 2.5 x 10(3) CFU of this organism or with 2.5 x 10(4) CFU of Mycobacterium bovis BCG. Groups of infected guinea pigs were skin tested with fractions of se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.804-810.1995
更新日期:1995-03-01 00:00:00
abstract::We demonstrate that 2-month-old female B10.T(6R) mice are highly resistant to systemic infection with the KIM5 strain of Yersinia pestis and that B10.T(6R) mice become susceptible to Y. pestis infection by the age of 5 months. In this study, young (2-month-old) and middle-aged (5- to 12-month-old) B10.T(6R) mice were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05267-11
更新日期:2011-11-01 00:00:00
abstract::A crude complex containing group C polysaccharide, outer membrane proteins, and lipopolysaccharide (LPS) was isolated from the cell-free culture liquid of Neisseria meningitidis serogroup C, serotype 2a. Group C polysaccharide and LPS were removed from this complex, resulting in an outer membrane complex and a purifie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.369-380.1983
更新日期:1983-04-01 00:00:00
abstract::In the progression of the life cycle of Plasmodium falciparum, a small proportion of asexual parasites differentiate into male or female sexual forms called gametocytes. Just like their asexual counterparts, gametocytes are contained within the infected host's erythrocytes (RBCs). However, unlike their asexual partner...
journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.00775-18
更新日期:2019-05-21 00:00:00
abstract::The role of glycosylinositol phospholipid 1 (GIPL-1) of Leishmania (Leishmania) major in the interaction of promastigotes and amastigotes with macrophages was analyzed. Monoclonal antibody MEST-1, which recognizes glycolipids containing terminal galactofuranose (Galf) residues (E. Suzuki, M. S. Toledo, H. K. Takahashi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.6592-6596.2002
更新日期:2002-12-01 00:00:00
abstract::Human serum at low concentrations inhibits the growth of Cryptococcus neoformans in vitro. Fractionation of serum yielded a purified inhibitory protein with a molecular mass of approximately 81.8 kDa, a pI of approximately 6.2, and an amino acid sequence that matched that of human transferrin. The inhibitory activity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3787-3791.2000
更新日期:2000-06-01 00:00:00
abstract::A hybridoma produced by the polyethylene glycol fusion of the NS-1 variant of the P3x63Ag8 BALB/c plasmacytoma to splenocytes harvested from a BALB/c mouse immunized with whole gonococci was found to be producing antibody to a common region on gonococcal lipopolysaccharide (LPS). Enzyme-linked immunosorbent assay inhi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.751-756.1981
更新日期:1981-12-01 00:00:00
abstract::A cytoplasmic 75-kDa immunogen from Chlamydia trachomatis serovar L2 has previously been characterized as being similar to the Escherichia coli heat shock protein DnaK. We have localized a linear epitope for one monoclonal antibody specific for C. trachomatis DnaK. By use of a recombinant DNA technique, the epitope wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.2051-2057.1994
更新日期:1994-05-01 00:00:00
abstract::Fimbriae are colonization factors of the human pathogen Haemophilus influenzae in that they mediate bacterial adherence to human eukaryotic cells. The contribution of the major (HifA) and putative minor (HifD and HifE) subunits of H. influenzae fimbriae to fimbria-specific adherence was studied by using mutants that w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4883-4889.1995
更新日期:1995-12-01 00:00:00
abstract::Proteolysis is a common microbial virulence mechanism that enables the destruction of host tissue and evasion from host defense mechanisms. Antimicrobial peptides, also known as host defense peptides, are effector molecules of the innate immunity that demonstrate a broad range of antimicrobial and immunoregulatory act...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00648-09
更新日期:2009-12-01 00:00:00
abstract::Adherence to fibrinogen and fibronectin plays a crucial role in Staphylococcus aureus experimental endocarditis. Previous genetic studies have shown that infection and carriage isolates do not systematically differ in their virulence-related genes, including genes conferring adherence, such as clfA and fnbA. We set ou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01274-09
更新日期:2010-04-01 00:00:00