Abstract:
:Little data are available regarding L-form phagocyte interactions. Due to difficulty with more conventional methods, a serum-free technique of surface phagocytosis employing cellulose ester micropore filters, fluorochrome staining, and reflecting fluorescence microscopy was developed. Intracellular localization of L-frms was confirmed by electron microscopy. In timed experiments, phagocytosis of a group D Streptococcus (ATCC 23241) and its L-form (T53I) were maximal at 30 min, although the bacteria were consistently better phagocyted. Phagocytosis of an Escherichia coli (strain Yale) and its L-form was also demonstrated with the same technique. The lesser phagocytosis of L-forms may be related to their lower chemotactic activity.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Harwick HJ,Barajas L,Montgomerie JZ,Kalmanson GM,Guze LBdoi
10.1128/IAI.5.6.976-981.1972subject
Has Abstractpub_date
1972-06-01 00:00:00pages
976-81issue
6eissn
0019-9567issn
1098-5522journal_volume
5pub_type
杂志文章abstract::Paracoccidioidomycosis patients show hyperactive humoral immune responses. Consequently, we investigated whether cytokines in supernatants from Paracoccidioides brasiliensis-stimulated gamma/delta T cells support B-cell activation. We detected proliferation of B cells and increased immunoglobulin M (IgM) and IgG produ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1608-1610.1995
更新日期:1995-04-01 00:00:00
abstract::Aspergillus fumigatus produces conidia that are highly dispersable and resistant to degradation. We have sought to analyze these properties by studying the rodlets which form the outer spore coat protein. Degenerate primers based on hydrophobins in other fungi were applied to genomic DNA from A. fumigatus in PCR. A pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4389-4395.1994
更新日期:1994-10-01 00:00:00
abstract::Effector mechanisms resulting from alternative complement pathway (ACP) activation cannot act efficiently against Yersinia enterocolitica serotype O3, as indicated by poor C3 to C9 consumption and by survival in EGTA (ethyleneglycoldiaminetetraacetic acid) Mg-serum. These results were not influenced by the lack or pre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.7.1984-1989.1989
更新日期:1989-07-01 00:00:00
abstract::As reported elsewhere, an enterotoxigenic strain of Escherichia coli serotype O25:K98:NM was epidemiologically incriminated as the etiological agent in a shipboard outbreak of diarrheal illness. This enterotoxigenic E. coli strain and possibly other enteric isolates were found to produce heat-labile toxin and not heat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.793-797.1979
更新日期:1979-06-01 00:00:00
abstract::Pathogens produce virulence factors that interact directly with host molecules, but in many cases the host targets are unknown. The genetic and molecular identification of these orphan targets is often not feasible with mammalian experimental models. However, a substantial number of known targets are molecules and pat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.10.6271-6275.2001
更新日期:2001-10-01 00:00:00
abstract::The morphology and pathology of cultured mouse glomeruli were examined at the cellular and subcellular levels after infection with a physiological isotonic L-form of Streptococcus pyogenes type 12 or exposure to streptococcal lipoteichoic acid. These changes, as viewed by light microscopy, were identical regardless of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1144-1151.1983
更新日期:1983-12-01 00:00:00
abstract::The mechanisms of immunity to salmonellae conferred by immunization with live vaccines were studied by adoptive transfer using the mouse-virulent strain Salmonella typhimurium C5 and innately susceptible BALB/c (ltys) mice. This organism cannot establish a sublethal infection in naive BALB/c mice. Animals immunized 2 ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3981-3984.1993
更新日期:1993-09-01 00:00:00
abstract::Antimicrobial peptides play an important role in host defense against Vibrio cholerae Generally, the V. cholerae O1 classical biotype is polymyxin B (PB) sensitive and El Tor is relatively resistant. Detection of classical biotype traits like the production of classical cholera toxin and PB sensitivity in El Tor strai...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00080-20
更新日期:2020-04-20 00:00:00
abstract::A procedure was developed to purify dermonecrotic toxin (DNT) from a sonic extract of a serotype D strain of Pasteurella multocida. Sonic extract containing DNT was applied to a DEAE-Sephacel column and eluted by a linear gradient of NaCl. Upon rechromatographing, fractions with dermonecrotic activity for guinea pigs ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.429-434.1984
更新日期:1984-11-01 00:00:00
abstract::One aspect in a broad spectrum of possible mechanisms of cariostatic reactions of fluoride is its interaction with the metabolism of oral bacteria. Information on the mechanisms and kinetics of fluoride inhibition of essential enzymes of the glycolytic pathway of the relevant bacteria is lacking. In this work, the iso...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.1043-1047.1990
更新日期:1990-04-01 00:00:00
abstract::Fibronectin-binding proteins mediate Staphylococcus aureus internalization into nonphagocytic cells in vitro. We have investigated whether fibronectin-binding proteins are virulence factors in the pathogenesis of pneumonia by using S. aureus strain 8325-4 and isogenic mutants in which fibronectin-binding proteins were...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3865-3873.2002
更新日期:2002-07-01 00:00:00
abstract::Tissues and organs provide the structural and biochemical landscapes upon which microbial pathogens and commensals function to regulate health and disease. While flat two-dimensional (2-D) monolayers composed of a single cell type have provided important insight into understanding host-pathogen interactions and infect...
journal_title:Infection and immunity
pub_type: 历史文章,杂志文章,评审
doi:10.1128/IAI.00282-18
更新日期:2018-10-25 00:00:00
abstract::The intestinal mucosa forms the first line of defense against infections mediated by enteric pathogens such as salmonellae. Here we exploited intestinal "organoids" (iHOs) generated from human induced pluripotent stem cells (hIPSCs) to explore the interaction of Salmonella enterica serovar Typhimurium with iHOs. Imagi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00161-15
更新日期:2015-07-01 00:00:00
abstract::The mechanism by which hamsters acquire resistance to yaws or frambesia is poorly understood. This investigation has shown that immune lymphoid cells (spleen and lymph node) could confer on hamsters resistance to infection with Treponema pertenue. Treatment of these immune cells with a specific antithymocyte serum (AT...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.1.208-212.1979
更新日期:1979-07-01 00:00:00
abstract::We report on the identification of a surface-exposed, highly conserved, immunogenic nontypeable Haemophilus influenzae (NTHi) protein, which elicits cross-reactive bactericidal antibodies against NTHi. The protein was extracted from NTHi strain P860295 with KSCN and purified; it migrated as a single band on a sodium d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2525-2534.2000
更新日期:2000-05-01 00:00:00
abstract::Serotype b-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans Y4 consists of D-fucose and L-rhamnose. To clarify the role of SPA in phagocytosis of the organism by human polymorphonuclear leukocytes (PMNs), monoclonal antibodies (MAbs) against SPA and SPA-defective mutants, which were constr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4589-4594.1995
更新日期:1995-12-01 00:00:00
abstract::We compared a cryptococcal culture filtrate antigen referred to as CneF with chemically defined cryptococcal antigen fractions isolated by Cherniak and co-workers by using double immunodiffusion gels, polyacrylamide gel electrophoresis, immunoblots, and footpad reactivity of immunized mice. The three previously descri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.2.424-431.1988
更新日期:1988-02-01 00:00:00
abstract::The diphtheria toxin repressor (DtxR) is an iron-dependent regulator of diphtheria toxin production and iron uptake in Corynebacterium diphtheriae. It is activated in vitro by divalent metal ions including Fe2+, Cd2+, Co2+, Mn2+, Ni2+, and Zn2+. We characterized 20 different mutations in dtxR induced by bisulfite muta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1600-1608.1994
更新日期:1994-05-01 00:00:00
abstract::In this work, apolipoprotein A-I (ApoA-I) was purified from pig sera. The responses of this protein after sterile inflammation and in animals infected with Actinobacillus pleuropneumoniae or Streptococcus suis were investigated. Decreases in the concentrations of ApoA-I, two to five times lower than the initial values...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.3184-3187.2005
更新日期:2005-05-01 00:00:00
abstract::Yersinia enterocolitica serotype 9 contained an antigenic component giving a reaction of total identity with Brucella native hapten and polysaccharide B. This component was present in a phenol-water extract (fraction 5; M. Redfearn, Ph.D. Thesis, University of Wisconsin, Madison, 1960) along with the smooth lipopolysa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.778-780.1982
更新日期:1982-11-01 00:00:00
abstract::Trehalose 6,6'-dimycolate (TDM) plays important roles in the development of granulomatous inflammation during infection with Mycobacterium spp., Rhodococcus spp., etc. To reveal the augmenting effect of TDM on vascular endothelial growth factor (VEGF) production and neovascularization, we investigated murine granuloma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2043-2052.2000
更新日期:2000-04-01 00:00:00
abstract::Cryptosporidium parvum is a coccidian parasite that causes diarrheal disease in many vertebrate species, including young (less than or equal to 1 month old) calves. Older calves and adult cattle are resistant to infection. In this study, newborn calves were raised in isolation from C. parvum for 1 week to 3 months bef...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2237-2240.1990
更新日期:1990-07-01 00:00:00
abstract::Passive transfer of antibody may be useful for preexposure prophylaxis against biological agents used as weapons of terror, such as Bacillus anthracis. Studies were performed to evaluate the ability of anthrax antiprotective antigen (anti-PA) and antilethal factor (anti-LF) neutralizing monoclonal antibodies (mAbs) to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00529-07
更新日期:2007-11-01 00:00:00
abstract::The role of glycosylinositol phospholipid 1 (GIPL-1) of Leishmania (Leishmania) major in the interaction of promastigotes and amastigotes with macrophages was analyzed. Monoclonal antibody MEST-1, which recognizes glycolipids containing terminal galactofuranose (Galf) residues (E. Suzuki, M. S. Toledo, H. K. Takahashi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.6592-6596.2002
更新日期:2002-12-01 00:00:00
abstract::MAEBL is a type 1 membrane protein that is implicated in the merozoite invasion of erythrocytes and sporozoite invasion of mosquito salivary glands. This apical organelle protein is structurally similar to the ebl erythrocyte binding proteins, such as EBA-175, except that the tandem ligand domains of MAEBL are similar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.6.3604-3608.2004
更新日期:2004-06-01 00:00:00
abstract::In a previous report (Y. Ohmori, S. Hanazawa, S. Amano, T. Miyoshi, K. Hirose, and S. Kitano, infect. Immun. 55:947-954, 1987), we showed that human gingival fibroblasts spontaneously produce thymocyte-activating factor (FTAF), which stimulates mitogen-induced thymocyte proliferation. In the present study, we examined...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.1.272-274.1988
更新日期:1988-01-01 00:00:00
abstract::Shiga toxigenic Escherichia coli (STEC) strains are a diverse group of organisms capable of causing severe gastrointestinal disease in humans. Within the STEC family, eae-positive STEC strains, particularly those belonging to serogroups O157 and O111, appear to have greater virulence for humans. However, in spite of b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.5930-5937.1999
更新日期:1999-11-01 00:00:00
abstract::Endogenous interleukin-12 (IL-12) mediates protection against Yersinia enterocolitica in C57BL/6 mice by triggering gamma interferon (IFN-gamma) production in NK and CD4+ T cells. Administration of exogenous IL-12 confers protection against yersiniae in Yersinia-susceptible BALB/c mice but exacerbates yersiniosis in r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.2213-2220.1998
更新日期:1998-05-01 00:00:00
abstract::The innate immune system recognizes pathogen-associated molecular patterns (PAMPs) through pattern recognition receptors (PRR) and transduces downstream signaling to activate the host defense. Here we report that in addition to direct PAMP-PRR interactions, live Candida albicans cells can release soluble factors to ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01041-09
更新日期:2010-01-01 00:00:00
abstract::Vaccination with recombinant outer surface protein A (OspA) has been shown to protect mice from infection with Borrelia burgdorferi, the Lyme disease agent. To determine whether antibodies to B. burgdorferi proteins other than OspA are involved in protective immunity, antibodies to OspA were removed from protective an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.2.657-661.1992
更新日期:1992-02-01 00:00:00