Abstract:
:We report on the identification of a surface-exposed, highly conserved, immunogenic nontypeable Haemophilus influenzae (NTHi) protein, which elicits cross-reactive bactericidal antibodies against NTHi. The protein was extracted from NTHi strain P860295 with KSCN and purified; it migrated as a single band on a sodium dodecyl sulfate-polyacrylamide gel with an apparent molecular mass of 63 kDa. Mouse antiserum generated against the purified protein was reactive on whole-cell enzyme-linked immunosorbent assay (ELISA) with seven NTHi strains and type b Eagan and Whittier strains and exhibited bactericidal activity to homologous and heterologous NTHi strains. However, the protein is made in small amounts in NTHi as corroborated by immunoelectron microscopy. To further study this protein, we cloned, sequenced, and expressed it recombinantly in Escherichia coli. The recombinant protein is localized in the periplasm of E. coli and has been purified to homogeneity. Both the recombinant and native proteins possess 5'-nucleotidase activity; hence, the protein has been called NucA. Mouse antiserum directed against the recombinant NucA protein was reactive on Western immunoblots and whole-cell ELISA with all H. influenzae strains tested including Eagan and was bactericidal for two heterologous strains tested. The antiserum also resulted in a log reduction in bacteremia, in an infant-rat protection study with H. influenzae type b as the challenge strain. These features suggest that NucA is a potential subunit vaccine candidate against NTHi disease.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Zagursky RJ,Ooi P,Jones KF,Fiske MJ,Smith RP,Green BAdoi
10.1128/iai.68.5.2525-2534.2000keywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
2525-34issue
5eissn
0019-9567issn
1098-5522journal_volume
68pub_type
杂志文章abstract::Conjugated polysaccharide vaccines protect against serogroup C meningococci. However, this approach cannot be applied to serogroup B, which is still a major cause of meningitis. We evaluated the immunogenicity of three surface-exposed proteins from serogroup B Neisseria meningitidis (App, NhhA, and NadA) identified du...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.4052-4060.2004
更新日期:2004-07-01 00:00:00
abstract::The short-term kinetics of fluoride uptake by cells from 20- to 22-h cultures of Streptococcus mutans strain 6715 were studied using rapid filtration and centrifugation techniques. Saline-suspended organisms were diluted with fluoride-containing solutions buffered at four different pH values (2.0, 4.0, 5.5, and 8.2). ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.3.680-687.1977
更新日期:1977-12-01 00:00:00
abstract::Mucosal exposure to Escherichia coli elicits an inflammatory response in the urinary tract. Interleukin-6 (IL-6) is secreted into the urine, and polymorphonuclear leukocytes (PMNLs) are recruited to the site of infection. This study analyzed the ability of mucosally administered bacterial components to activate IL-6 a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.12.4357-4362.1991
更新日期:1991-12-01 00:00:00
abstract::Female C57BL/6J mice were infected with Trypanosoma cruzi and subsequently given macrophages or lymphocytes from syngeneic donors which had recovered from the acute infection. Mice which received immune peritoneal macrophages, splenic lymphocytes, or lymph node lymphocytes developed lower mean parasitemias and cumulat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.3.838-843.1979
更新日期:1979-09-01 00:00:00
abstract::The search to identify Mycobacterium tuberculosis antigens capable of conferring protective immunity against tuberculosis has received a boost owing to the resurgence of tuberculosis over the past two decades. It has long been recognized that lymphoid cells are required for protection against M. tuberculosis. While tr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.1929-1937.2003
更新日期:2003-04-01 00:00:00
abstract::Peptic ulcer disease (PUD) occurs after a long-term Helicobacter pylori infection. However, the disease can develop earlier, and rare cases have been observed in children, suggesting that these H. pylori strains may be more virulent. We used suppressive subtractive hybridization for comparative genomics between H. pyl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00123-06
更新日期:2006-07-01 00:00:00
abstract::Immunoglobulin A (IgA) was prepared from samples of parotid saliva collected from two individuals over a 150-day period. Standardized solutions of the IgA were tested for agglutinating antibody activity against strains of streptococcus salivarius, S. sanguis, S. mitis, and S. mutans that had been isolated from a homol...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.3.603-606.1975
更新日期:1975-03-01 00:00:00
abstract::Using microarray technology, we studied the early differential expression of 3,528 genes in human meningothelial cells in response to meningococcal challenge. Thirty-two genes were up-regulated, and four were down-regulated. Those up-regulated included the tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-8 (b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2718-2722.2001
更新日期:2001-04-01 00:00:00
abstract::Streptococcal pyrogenic exotoxin B (SpeB), a conserved cysteine protease expressed by virtually all Streptococcus pyogenes strains, has recently been shown to be an important virulence factor (S. Lukomski, S. Sreevatsan, C. Amberg, W. Reichardt, M. Woischnik, A. Podbielski, and J. M. Musser, J. Clin. Invest. 99:2574-2...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.771-776.1998
更新日期:1998-02-01 00:00:00
abstract::Advanced human periodontitis is considered to be a B-cell lesion, but the cellular infiltrate contains several cell types, the distribution of which has not been determined. This experiment was designed to characterize and identify the immunocompetent cells on histological sections and in eluates from diseased human g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.1.365-374.1983
更新日期:1983-07-01 00:00:00
abstract::Streptolysin-O (SLO) is a thiol-activated, membrane-damaging protein toxin of Mr 69,000 that is produced by most strains of beta-hemolytic group A streptococci. Native, primarily water-soluble toxin molecules bind to cholesterol-containing target membranes to assemble into supramolecular curved rod structures (25 to 1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.52-60.1985
更新日期:1985-01-01 00:00:00
abstract::Utilization of the technique of immune electron microscopy has enabled the detection of a coronavirus in organ culture harvests derived from a washing from an adult with an acute upper respiratory tract illness; convalescent serum was the source of specific antibody. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.1.111-116.1973
更新日期:1973-01-01 00:00:00
abstract::The adherence of 58 nontypeable Haemophilus influenzae isolates obtained from patients with otitis media or chronic obstructive pulmonary disease (COPD) and obtained from the throats of healthy individuals to Chang and NCI-H292 epithelial cells was compared. Otitis media isolates, but not COPD isolates, adhered signif...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.8.4658-4665.2000
更新日期:2000-08-01 00:00:00
abstract::Serial passage of Cryptococcus neoformans in mice increases virulence relative to the nonpassaged line. Postpassaged lines showed no difference in the expression of most known virulence factors, with the exception that the more virulent lines had smaller capsules in vitro. These data imply that other mechanisms of vir...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.7047-7050.2005
更新日期:2005-10-01 00:00:00
abstract::The results of this study show that recombinant interleukin-8 (IL-8) enhances the intracellular killing of Mycobacterium fortuitum by human granulocytes. This chemokine did not stimulate the phagocytosis of M. fortuitum by granulocytes at various bacterium-to-cell ratios. The killing process was not affected by the NA...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.8.3111-3116.1993
更新日期:1993-08-01 00:00:00
abstract::To explore the mechanisms underlying the eosinophil-mediated inflammation of tropical pulmonary eosinophilia (TPE), bronchoalveolar lavage (BAL) fluid, serum, and supernatants from pulmonary and blood leukocytes (WBC) from patients with acute TPE (n = 6) were compared with those obtained from healthy uninfected indivi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1337-1342.2003
更新日期:2003-03-01 00:00:00
abstract::Porphyromonas gingivalis is a periodontal pathogen capable of invading primary cultures of normal human gingival epithelial cells (NHGEC). Involvement of P. gingivalis fimbriae in the invasion process was examined. Purified P. gingivalis 33277 fimbriae blocked invasion of this organism into NHGEC in a dose-dependent m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.1.313-316.1997
更新日期:1997-01-01 00:00:00
abstract::In mice infected with virulent blood (trypomastigote) forms of Trypanosoma cruzi, complement depletion with cobra venom factor caused a marked exacerbation of the disease evidenced by significantly increased parasitemia levels and early mortality as compared with those of untreated infected animals. The effect was gre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.86-91.1975
更新日期:1975-01-01 00:00:00
abstract::Bacterial lipopolysaccharide (LPS) is a potent stimulus of cells, yet a target protein for LPS has not been defined. We used two approaches to define LPS-binding sites on cell surfaces: one assay measured binding of LPS-coated erythrocytes (ELPS) to cultured human cells, and a second measured binding of a radiolabeled...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.12.4069-4075.1990
更新日期:1990-12-01 00:00:00
abstract::Upon infection of a host, the pathogenic fungus Aspergillus fumigatus is attacked by the reactive oxygen species produced by phagocytic cells. Detoxification of hydrogen peroxide by catalases was proposed as a way to overcome this host response. A. fumigatus produces three active catalases; one is produced by conidia,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.6.3551-3562.2003
更新日期:2003-06-01 00:00:00
abstract::Galactomannan (GM) extracted from mycelia of Aspergillus fumigatus with cold dilute alkali reacted with antiserum specific for an antigen that circulated in invasive aspergillosis in rabbits and humans. The GM was purified by its affinity for concanavalin A and was separated from a nonantigenic glucan by gel permeatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.1.357-365.1979
更新日期:1979-07-01 00:00:00
abstract::Inactivated Marek's disease virus-infected chicken kidney cells and inactivated MSB-1 lymphoblastoid Marek's disease tumor cells were used to immunize chickens as virus- and tumor-associated antigens, respectively. Immune and nonimmune birds were then challenged by exposure to live virulent Marek's disease virus. Both...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.2.547-553.1979
更新日期:1979-11-01 00:00:00
abstract::Killing of Salmonella minnesota and Salmonella typhimurium S and R strains in serum of nonimmune humans and guinea pigs was drastically reduced in the selective absence of C1q, C1r, Ca2+, C4, or C2, the components of the classical complement pathway. Binding of C1 and C1q to the S form and six different core-deficient...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.935-939.1982
更新日期:1982-09-01 00:00:00
abstract::In studies of immunity to malaria, the absence of febrile malaria is commonly considered evidence of "protection." However, apparent "protection" may be due to a lack of exposure to infective mosquito bites or due to immunity. We studied a cohort that was given curative antimalarials before monitoring began and docume...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01358-08
更新日期:2009-05-01 00:00:00
abstract::The effects of 1,25-dihydroxy vitamin D3 and gamma interferon on the phenotypic changes associated with monocyte maturation in vitro were investigated. Human monocytes separated from peripheral blood mononuclear cell populations by adherence to plastic were cultured for 7 days on glass. Immunocytological analysis was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2017-2020.1987
更新日期:1987-09-01 00:00:00
abstract::We examined the association of Herpesvirus saimiri with lymphocyte subsets and the functional integrity and distribution of these populations in owl monkeys with chronic, disease-free infections, in uninoculated, control animals, and in one monkey with H. saimiri-induced lymphoma. The lymphocyte subpopulations examine...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.698-706.1981
更新日期:1981-05-01 00:00:00
abstract::We identified, by two-dimensional electrophoretic analysis and microsequencing, a protein of Chlamydia trachomatis elementary bodies which corresponds to the polypeptide (pgp3) encoded by open reading frame 3 (ORF3). Amino acid analysis showed that the first residue (Gly) found in the native protein is the one encoded...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.12.5491-5497.1994
更新日期:1994-12-01 00:00:00
abstract::The glycolipid haptens of Mycoplasma pneumoniae became immunogenic when bound to membrane proteins of Acholeplasma laidlawii by reaggregation. This process consisted of the solubilization of lipid-depleted A. laidlawii membranes and M. pneumoniae glycolipids in 20 mm sodium dodecyl sulfate and dialysis of the mixed so...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.3.420-423.1971
更新日期:1971-03-01 00:00:00
abstract::This study has demonstrated the extreme sensitivity of Chlamydia trachomatis growing in McCoy cells to small changes in external amino acid supply. In the absence of cycloheximide, a decrease in the amino acid concentration of medium to 75% of control values was sufficient to induce the growth of enlarged chlamydial f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1457-1464.2000
更新日期:2000-03-01 00:00:00
abstract::The immune response of mice to live, heat-killed, or autoclaved Mycobacterium leprae was investigated. After sensitization with 10(7) organisms in each group, recipient mice were transfused with the sensitized splenocytes 28 days later. A selected number of these mice were infected with 5 X 10(3) M. leprae, and the re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.79-83.1984
更新日期:1984-01-01 00:00:00