Abstract:
:Conjugated polysaccharide vaccines protect against serogroup C meningococci. However, this approach cannot be applied to serogroup B, which is still a major cause of meningitis. We evaluated the immunogenicity of three surface-exposed proteins from serogroup B Neisseria meningitidis (App, NhhA, and NadA) identified during whole-genome sequencing. Mice were immunized intranasally with individual proteins in the presence of wild-type Escherichia coli heat-labile enterotoxin (LTwt), LTR72, a partially inactivated mutant, or LTK63, a completely nontoxic mutant, as the adjuvant. Each of the meningococcal proteins induced significant cellular responses; NhhA and NadA induced strong antibody responses, but only NadA induced bactericidal antibody when administered intranasally with mucosal adjuvants. In addition, immunoglobulin A and bactericidal antibodies were detected in the respiratory tract following intranasal delivery of NadA. Analysis of antigen-specific cytokine production by T cells from immunized mice revealed that intranasal immunization with NadA alone failed to generate detectable cellular immune responses. In contrast, LTK63, LTR72, and LTwt significantly augmented NadA-specific gamma interferon, interleukin-4 (IL-4), IL-5, and IL-10 production by spleen and lymph node cells, suggesting that both Th1 and Th2 cells were induced in vivo. The strongest cellular responses and highest bactericidal antibody titers were generated with LTR72 as the adjuvant. These findings demonstrate that the quality and magnitude of the immune responses generated by mucosal vaccines are influenced by the antigen as well as the adjuvant and suggest that nasal delivery of NadA with mucosal adjuvants has considerable potential in the development of a mucosal vaccine against serogroup B meningococci.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Bowe F,Lavelle EC,McNeela EA,Hale C,Clare S,Arico B,Giuliani MM,Rae A,Huett A,Rappuoli R,Dougan G,Mills KHdoi
10.1128/IAI.72.7.4052-4060.2004keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
4052-60issue
7eissn
0019-9567issn
1098-5522pii
72/7/4052journal_volume
72pub_type
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journal_title:Infection and immunity
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doi:10.1128/IAI.35.3.759-763.1982
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.2.402-407.1977
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.130-135.1981
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1981-01-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1995-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.2.421-427.1986
更新日期:1986-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1114-1116.1984
更新日期:1984-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1988-04-01 00:00:00