Abstract:
:Invasive aspergillosis is a serious fungal infection caused by the proliferation and invasion of Aspergillus hyphae in tissue. Neutrophils (PMNs) are the most important line of defense against Aspergillus hyphae. To investigate the role of granulocyte colony-stimulating factor (G-CSF) and gamma interferon (IFN-gamma) against Aspergillus fumigatus, we studied the effects of the two cytokines on the oxidative burst and the capacity of normal human PMNs to damage hyphae of the organism. G-CSF enhanced PMN oxidative burst measured as superoxide anion (O2-) production in response to N-formylmethionyl leucyl phenylalanine, serum opsonized hyphae, and nonopsonized hyphae by 75, 37, and 24%, respectively, compared with control PMNs (P < 0.015). IFN-gamma also induced increases of 52, 71, and 96%, respectively, in response to the same stimuli (P < 0.006). In addition, the capacity of PMNs to damage hyphae as measured by the 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MMT) colorimetric metabolic assay was significantly enhanced by G-CSF and IFN-gamma (P < 0.01 and < 0.05, respectively). The enhancement was achieved irrespective of serum opsonization of the hyphae, suggesting upregulatory actions of the two cytokines on signal pathways specific for opsonized and nonopsonized hyphae. The combination of the two cytokines exhibited an additive effect at the higher concentrations compared with the effects of the cytokines alone (P < 0.05). Pretreatment of PMNs with protein synthesis inhibitors showed that IFN-gamma activates PMN function through transcriptional regulation, whereas the effect of G-CSF does not require new proteins. These in vitro effects suggest modulatory roles for G-CSF and IFN-gamma in the host defense against Aspergillus hyphae irrespective of serum opsonization and a potential utility of the cytokines as adjuncts for the prevention and possible treatment of invasive aspergillosis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Roilides E,Uhlig K,Venzon D,Pizzo PA,Walsh TJdoi
10.1128/IAI.61.4.1185-1193.1993subject
Has Abstractpub_date
1993-04-01 00:00:00pages
1185-93issue
4eissn
0019-9567issn
1098-5522journal_volume
61pub_type
杂志文章abstract::Bordetella pertussis 165-9K/129G, which produces a nontoxic form of pertussis toxin (PT), was used to prepare a whole-cell diphtheria-tetanus-pertussis (DTP) vaccine. The in vivo potency and the serological response induced by this vaccine were comparable to those of the conventional DTP vaccine which contains active ...
journal_title:Infection and immunity
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doi:10.1128/IAI.60.3.1150-1155.1992
更新日期:1992-03-01 00:00:00
abstract::The cell surface of Chlamydia psittaci seems important for establishing infection since (i) UV-treated elementary bodies (EB) attach to and are ingested by L cells and (ii) heat or antibody treatment decreases attachment to L cells and promotes the fusion of chlamydiae-containing phagosomes with lysosomes in macrophag...
journal_title:Infection and immunity
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doi:10.1128/IAI.40.2.741-751.1983
更新日期:1983-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.374-384.1983
更新日期:1983-10-01 00:00:00
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更新日期:2003-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.2917-2929.1994
更新日期:1994-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.5.686-688.1970
更新日期:1970-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.12.4606-4609.1991
更新日期:1991-12-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1986-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-03-23 00:00:00
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更新日期:2012-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.11.3331-3337.1989
更新日期:1989-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.8.1924-1926.1987
更新日期:1987-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.72.11.6586-6588.2004
更新日期:2004-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.10.4192-4199.1992
更新日期:1992-10-01 00:00:00
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journal_title:Infection and immunity
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doi:
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2004-01-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1995-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-04-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1997-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1990-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1988-07-01 00:00:00
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journal_title:Infection and immunity
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