Gonococcal opacity protein promotes bacterial entry-associated rearrangements of the epithelial cell actin cytoskeleton.


:Neisseria gonorrhoeae enters cultured human mucosal cells following binding of a distinct gonococcal opacity (Opa) outer membrane protein to cell surface proteoglycan receptors. We examined the route of internalization that is activated by Opa-expressing gonococci (strain VP1). Microscopy of infected Chang epithelial cells showed that gonococcal uptake was insensitive to monodansylcadaverine (150 microM), which interferes with clathrin-mediated endocytosis. Similarly, indirect immunofluorescence staining for clathrin in infected cells showed distribution of cellular clathrin unaltered from the distribution in noninfected cells. The microtubule inhibitors colchicine (50 microM) and nocodazole (20 microM) but not the microtubule-stabilizing agent taxol (10 microM) caused a moderate (30 to 50%) reduction in gonococcal entry without affecting bacterial adherence. The most dramatic effects were obtained with the microfilament-disrupting agent cytochalasin D (3 microM), which totally blocked bacterial entry into the cells. Double immunofluorescence staining of gonococci and actin filaments in infected cells demonstrated bacterium-associated accumulations of F-actin as an early signal of bacterial entry. The recruitment of F-actin was transient and disappeared once the bacteria were inside the cells. Cytochalasin D disrupted the actin cytoskeleton architecture but did not prevent the recruitment of F-actin by the bacteria. Adherent, noninvasive gonococcal Opa variants lacked the ability to mobilize F-actin. Recombinant Escherichia coli expressing the gonococcal invasion-promoting Opa of gonococcal strain MS11 (Opa50) adhered to the epithelial cells in an Opa-dependent fashion but was not internalized and did not recruit detectable amounts of F-actin. Coinfection with the E. coli recombinant strain and gonococci resulted in specific entry of the diplococci, despite the presence of large numbers of adherent E. coli cells. Together, our results indicate that Opa-mediated gonococcal entry into Chang cells resembles phagocytosis rather than macropinocytosis reported for Salmonella spp. and sequentially involves gonococcal adherence to the cell surface, Opa-dependent and cytochalasin-insensitive recruitment of F-actin, and cytochalasin D-sensitive bacterial internalization.


Infect Immun


Infection and immunity


Grassmé HU,Ireland RM,van Putten JP




Has Abstract


1996-05-01 00:00:00












  • Surface-expressed mig protein protects Streptococcus dysgalactiae against phagocytosis by bovine neutrophils.

    abstract::The mig gene of Streptococcus dysgalactiae, a major bovine mastitis pathogen, encodes two plasma protein-binding receptors, alpha2-macroglobulin (alpha2-M) and immunoglobulin G (IgG). In this study, the mig gene from one S. dysgalactiae isolate was cloned and expressed in Escherichia coli. The IgG receptor region enco...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Song XM,Perez-Casal J,Bolton A,Potter AA

    更新日期:2001-10-01 00:00:00

  • Isolation, characterization, and inhibition kinetics of enolase from Streptococcus rattus FA-1.

    abstract::One aspect in a broad spectrum of possible mechanisms of cariostatic reactions of fluoride is its interaction with the metabolism of oral bacteria. Information on the mechanisms and kinetics of fluoride inhibition of essential enzymes of the glycolytic pathway of the relevant bacteria is lacking. In this work, the iso...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hüther FJ,Psarros N,Duschner H

    更新日期:1990-04-01 00:00:00

  • Mumps class-specific immunoglobulins in radioimmunoassay and conventional serology.

    abstract::Antibodies against mumps virus have been studied by using immunoglobulin class-specific indicators labeled with (125)I in the radioimmunoassay (RIA) procedure. The immunoglobulins in paired acute and convalescent sera were allowed to react with mumps virus in a solid-phase RIA system. Class-specific immunoglobulin ind...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Daugharty H,Warfield DT,Hemingway WD,Casey HL

    更新日期:1973-03-01 00:00:00

  • Analysis of the human antibody response to thrombospondin-related anonymous protein of Plasmodium falciparum.

    abstract::Thrombospondin-related anonymous protein (TRAP) of the malaria parasite Plasmodium falciparum shares two sequence motifs with other proteins which possess adhesive properties. Recently, findings indicate that TRAP is an antigen which contributes to antisporozoite immunity. We have cloned and expressed the TRAP coding ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Scarselli E,Tolle R,Koita O,Diallo M,Müller HM,Früh K,Doumbo O,Crisanti A,Bujard H

    更新日期:1993-08-01 00:00:00

  • Preparation and physicochemical and immunological characterization of polysaccharide-outer membrane protein complexes of Neisseria meningitidis.

    abstract::A crude complex containing group C polysaccharide, outer membrane proteins, and lipopolysaccharide (LPS) was isolated from the cell-free culture liquid of Neisseria meningitidis serogroup C, serotype 2a. Group C polysaccharide and LPS were removed from this complex, resulting in an outer membrane complex and a purifie...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Beuvery EC,Miedema F,van Delft RW,Haverkamp J,Leussink AB,te Pas BJ,Teppema KS,Tiesjema RH

    更新日期:1983-04-01 00:00:00

  • The SrtA Sortase of Streptococcus agalactiae is required for cell wall anchoring of proteins containing the LPXTG motif, for adhesion to epithelial cells, and for colonization of the mouse intestine.

    abstract::Streptococcus agalactiae (group B streptococcus [GBS]) is the leading cause of neonatal pneumonia, sepsis, and meningitis. An in silico genome analysis indicated that GBS strain NEM316 encodes 35 proteins containing an LPXTG motif which are thought to be covalently linked to the peptidoglycan by an enzyme called sorta...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lalioui L,Pellegrini E,Dramsi S,Baptista M,Bourgeois N,Doucet-Populaire F,Rusniok C,Zouine M,Glaser P,Kunst F,Poyart C,Trieu-Cuot P

    更新日期:2005-06-01 00:00:00

  • Clostridium perfringens enterotoxin damages the human intestine in vitro.

    abstract::In vitro, Clostridium perfringens enterotoxin (CPE) binds to human ileal epithelium and induces morphological damage concurrently with reduced short-circuit current, transepithelial resistance, and net water absorption. CPE also binds to the human colon in vitro but causes only slight morphological and transport chang...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fernández Miyakawa ME,Pistone Creydt V,Uzal FA,McClane BA,Ibarra C

    更新日期:2005-12-01 00:00:00

  • Immune responses of specific pathogen-free and gnotobiotic mice to antigens of indigenous and nonindigenous microorganisms.

    abstract::Strains of indigenous Escherichia coli, Bacteroides, and Lactobacillus were isolated from the gastrointestinal tracts of specific pathogen-free (SPF) mice. Nonvaccinated SPF mice exhibited in their spleens low numbers of plaque-forming cells (PFC) and rosette-forming cells reacting with antigens of these andigenous ba...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Berg RD,Savage DC

    更新日期:1975-02-01 00:00:00

  • Enhanced disease severity in Helicobacter pylori-infected mice deficient in Fas signaling.

    abstract::Recent evidence suggests that immune-mediated gastric epithelial cell apoptosis through Fas-Fas ligand interactions participates in Helicobacter pylori disease pathogenesis. To define the role of Fas signaling in vivo, H. pylori strain SS1 infection in C57BL/6 mice was compared to that in mice deficient in the Fas lig...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Jones NL,Day AS,Jennings H,Shannon PT,Galindo-Mata E,Sherman PM

    更新日期:2002-05-01 00:00:00

  • Effects of a phagocytosis-stimulating factor derived from polymorphonuclear neutrophils on the functions of macrophages.

    abstract::The effects of phagocytosis-stimulating factor (PSF) derived from polymorphonuclear neutrophils on macrophage functions were studied. PSF enhanced the initial rate of phagocytosis of serum-opsonized zymosan particles by macrophages, whereas it did not affect the phagocytosis of immunoglobulin G-sensitized and inert zy...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ishibashi Y,Yamashita T

    更新日期:1987-08-01 00:00:00

  • Humoral and cellular responses of mice to infection with a cold-adapted influenza A virus variant.

    abstract::The serum antibody response and four different cellular immune responses (cytotoxic T cells, delayed-type hypersensitivity T cells, natural killer cells, and cytotoxic macrophage levels) induced in CBA/H mice were measured at different times after intranasal inoculation of a cold-adapted (ca) variant of influenza A vi...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mak NK,Zhang YH,Ada GL,Tannock GA

    更新日期:1982-10-01 00:00:00

  • Killer cell lectin-like receptor G1 deficiency significantly enhances survival after Mycobacterium tuberculosis infection.

    abstract::The expression of T cell differentiation markers is known to increase during Mycobacterium tuberculosis infection, and yet the biological role of such markers remains unclear. We examined the requirement of the T cell differentiation marker killer cell lectin-like receptor G1 (KLRG1) during M. tuberculosis infection u...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Cyktor JC,Carruthers B,Stromberg P,Flaño E,Pircher H,Turner J

    更新日期:2013-04-01 00:00:00

  • Genetic characterization of DNA region containing the trh and ure genes of Vibrio parahaemolyticus.

    abstract::We have demonstrated that possession of the gene for thermostable direct hemolysin-related hemolysin (trh) coincides with the presence of the urease gene among clinical Vibrio parahaemolyticus strains and that the location of the two genes are in close proximity on the chromosome. Here, we cloned and sequenced the 15,...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Park KS,Iida T,Yamaichi Y,Oyagi T,Yamamoto K,Honda T

    更新日期:2000-10-01 00:00:00

  • Both hemolytic anemia and malaria parasite-specific factors increase susceptibility to Nontyphoidal Salmonella enterica serovar typhimurium infection in mice.

    abstract::Severe pediatric malaria is an important risk factor for developing disseminated infections with nontyphoidal Salmonella serotypes (NTS). While recent animal studies on this subject are lacking, early work suggests that an increased risk for developing systemic NTS infection during malaria is caused by hemolytic anemi...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Roux CM,Butler BP,Chau JY,Paixao TA,Cheung KW,Santos RL,Luckhart S,Tsolis RM

    更新日期:2010-04-01 00:00:00

  • Isolation and molecular characterization of spontaneously occurring cytolysin-negative mutants of Actinobacillus pleuropneumoniae serotype 7.

    abstract::Actinobacillus pleuropneumoniae serotype 7 strains are shown to spontaneously lose cytolytic activity with a frequency of approximately 10(-4). The phenotypic change is associated with the loss of approximately 8.5 kbp of chromosomal DNA. A genomic fragment encoding the cytolysin and its flanking sequences was cloned ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Anderson C,Potter AA,Gerlach GF

    更新日期:1991-11-01 00:00:00

  • Requirements for ingestion of Chlamydia psittaci by mouse fibroblasts (L cells).

    abstract::Ingestion of 14C-amino acid-labeled Chlamydia psittaci (6BC) by mouse fibroblasts (L cells) was inhibited when the host cells were incubated for 30 min at 37 degrees C in Earle salts containing 10 mug of crystalline trypsin per ml. Tryptic digestion also inhibited the ingestion of 1-mum polystrene latex beads. Trypsin...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Byrne GI

    更新日期:1976-09-01 00:00:00

  • Phagocytosis of Pseudomonas aeruginosa by polymorphonuclear leukocytes and monocytes: effect of cystic fibrosis serum.

    abstract::It has been shown previously that serum from chronically infected patients with cystic fibrosis inhibits the phagocytosis of Pseudomonas aeruginosa by both normal and cystic fibrosis alveolar macrophages. In the present study, the ability of peripheral monocytes and polymorphonuclear leukocytes from normal volunteers ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Thomassen MJ,Demko CA,Wood RE,Sherman JM

    更新日期:1982-11-01 00:00:00

  • Salmonella enterica serovar Typhimurium periplasmic superoxide dismutases SodCI and SodCII are required for protection against the phagocyte oxidative burst.

    abstract::Vitamin D(3) (1,25-dihydroxycholecalciferol) induced the phagocyte oxidative burst and intracellular killing of Salmonella enterica serovar Typhimurium in a phosphatidylinositol 3-kinase-dependent manner. The antimicrobial effect was more pronounced for Salmonella SodCI and SodCII mutants, confirming the role of the p...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sly LM,Guiney DG,Reiner NE

    更新日期:2002-09-01 00:00:00

  • Direct activation of human monocyte-derived macrophages by a bacterial glycoprotein extract inhibits the intracellular multiplication of virulent Legionella pneumophila serogroup 1.

    abstract::Intracellular multiplication of virulent Legionella pneumophila serogroup 1 was inhibited by human monocyte-derived macrophages activated by a glycoprotein extract of Klebsiella pneumoniae, RU 41.740. Macrophage cultures were infected with L. pneumophila in the presence of immune antibodies on day 7 of culture. Extrac...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rajagopalan P,Dournon E,Vildé JL,Pocidalo JJ

    更新日期:1987-09-01 00:00:00

  • New type of exfoliatin obtained from staphylococcal strains, belonging to phage groups other than group II, isolated from patients with impetigo and Ritter's disease.

    abstract::Four strains of Staphylococcus aureus of a phage type other than the second group, isolated from patients with impetigo and Ritter's disease, were found to produce an exotoxin similar to those reported by Melish et al. (1972), Kapral and Miller (1971), and Arbuthnott et al. (1973). This toxin could elicit a general ex...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kondo I,Sakurai S,Sarai Y

    更新日期:1974-10-01 00:00:00

  • Immunization of pigs to prevent disease in humans: construction and protective efficacy of a Salmonella enterica serovar Typhimurium live negative-marker vaccine.

    abstract::Zoonotic infections caused by Salmonella enterica serovar Typhimurium pose a constant threat to consumer health, with the pig being a particularly major source of multidrug-resistant isolates. Vaccination, as a promising approach to reduce colonization and shedding, has been scarcely used, as it interferes with curren...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Selke M,Meens J,Springer S,Frank R,Gerlach GF

    更新日期:2007-05-01 00:00:00

  • Passively released heme from hemoglobin and myoglobin is a potential source of nutrient iron for Bordetella bronchiseptica.

    abstract::Colonization by Bordetella bronchiseptica results in a variety of inflammatory respiratory infections, including canine kennel cough, porcine atrophic rhinitis, and a whooping cough-like disease in humans. For successful colonization, B. bronchiseptica must acquire iron (Fe) from the infected host. A vast amount of Fe...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mocny JC,Olson JS,Connell TD

    更新日期:2007-10-01 00:00:00

  • Masking of β(1-3)-glucan in the cell wall of Candida albicans from detection by innate immune cells depends on phosphatidylserine.

    abstract::The virulence of Candida albicans in a mouse model of invasive candidiasis is dependent on the phospholipids phosphatidylserine (PS) and phosphatidylethanolamine (PE). Disruption of the PS synthase gene CHO1 (i.e., cho1Δ/Δ) eliminates PS and blocks the de novo pathway for PE biosynthesis. In addition, the cho1Δ/Δ muta...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Davis SE,Hopke A,Minkin SC Jr,Montedonico AE,Wheeler RT,Reynolds TB

    更新日期:2014-10-01 00:00:00

  • Polymorphonuclear leukocyte chemotactic activity in rabbit serum and Guinea pig serum treated with immune complexes: evidence for c5a as the major chemotactic factor.

    abstract::A number of polymorphonuclear leukocyte (PMN) chemotactic factors derived from complement components have been described. We sought to determine which of these factors accounted for the majority of PMN chemotactic activity in rabbit serum and guinea pig serum treated with preformed immune complexes. Normal rabbit sera...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Snyderman R,Phillips J,Mergenhagen SE

    更新日期:1970-06-01 00:00:00

  • Glycoproteins of natural origin with an affinity for hepatitis B surface antigen.

    abstract::Sera from certain animal species contain a substance(s) which binds hepatitis B surface antigen. The hepatitis B binding substance found in animals is not antibody, but appears to be a glycoprotein which reacted with antigen-coated beads and produced a "false positive" test for antibody. This glycoprotein could be sel...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Millman I,McMichael JC

    更新日期:1978-09-01 00:00:00

  • Role of Escherichia coli alpha-hemolysin and bacterial adherence in infection: requirement for release of inflammatory mediators from granulocytes and mast cells.

    abstract::We investigated the role of bacterial mannose-resistant fimbriation of S fimbriae (Fim), mannose-resistant hemagglutination (S-Mrh), and hemolysin (Hly) production by an Escherichia coli parent and genetically cloned strains as regards their effect on histamine release from rat mast cells and generation of the chemilu...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: König B,König W,Scheffer J,Hacker J,Goebel W

    更新日期:1986-12-01 00:00:00

  • Different virulence of influenza A virus strains and susceptibility to pneumococcal otitis media in chinchillas.

    abstract::We have previously shown that chinchillas infected with a multiply passaged laboratory strain of influenza A/NWS/33 (H1N1) develop negative middle-ear pressure; polymorphonuclear leukocyte oxidative, bactericidal, and chemotactic dysfunction; and increased susceptibility to pneumococcal otitis media. Because influenza...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Giebink GS,Wright PF

    更新日期:1983-09-01 00:00:00

  • Mechanisms of immunity in typhus infections. IV. Failure of chicken embryo cells in culture to restrict growth of antibody-sensitized Rickettsia prowazeki.

    abstract::Rickettsia prowazeki, pretreated with typhus immune human serum, readily infects, and grows in, chicken embryo cells in culture. This finding is similar to those of previous studies which showed that typhus rickettsiae, pretreated with immune serum, grow in cells of the yolk sac of embryonated hen eggs and in the cell...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Wisseman CL Jr,Waddell AD,Walsh WT

    更新日期:1974-03-01 00:00:00

  • Differentiation of F38 mycoplasmas causing contagious caprine pleuropneumonia with a growth-inhibiting monoclonal antibody.

    abstract::Monoclonal antibody WM-25 inhibited the in vitro growth of 13 F38 isolates from goats with contagious caprine pleuropneumonia but not 7 heterologous mycoplasma isolates representing four different species. In contrast to results with polyclonal antisera, growth inhibition by monoclonal antibody WM-25 was specific for ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rurangirwa FR,McGuire TC,Musoke AJ,Kibor A

    更新日期:1987-12-01 00:00:00

  • Mechanism of fluconazole resistance in Candida albicans biofilms: phase-specific role of efflux pumps and membrane sterols.

    abstract::Candida albicans biofilms are formed through three distinct developmental phases and are associated with high fluconazole (FLU) resistance. In the present study, we used a set of isogenic Candida strains lacking one or more of the drug efflux pumps Cdr1p, Cdr2p, and Mdr1p to determine their role in FLU resistance of b...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mukherjee PK,Chandra J,Kuhn DM,Ghannoum MA

    更新日期:2003-08-01 00:00:00