Gonococcal opacity protein promotes bacterial entry-associated rearrangements of the epithelial cell actin cytoskeleton.


:Neisseria gonorrhoeae enters cultured human mucosal cells following binding of a distinct gonococcal opacity (Opa) outer membrane protein to cell surface proteoglycan receptors. We examined the route of internalization that is activated by Opa-expressing gonococci (strain VP1). Microscopy of infected Chang epithelial cells showed that gonococcal uptake was insensitive to monodansylcadaverine (150 microM), which interferes with clathrin-mediated endocytosis. Similarly, indirect immunofluorescence staining for clathrin in infected cells showed distribution of cellular clathrin unaltered from the distribution in noninfected cells. The microtubule inhibitors colchicine (50 microM) and nocodazole (20 microM) but not the microtubule-stabilizing agent taxol (10 microM) caused a moderate (30 to 50%) reduction in gonococcal entry without affecting bacterial adherence. The most dramatic effects were obtained with the microfilament-disrupting agent cytochalasin D (3 microM), which totally blocked bacterial entry into the cells. Double immunofluorescence staining of gonococci and actin filaments in infected cells demonstrated bacterium-associated accumulations of F-actin as an early signal of bacterial entry. The recruitment of F-actin was transient and disappeared once the bacteria were inside the cells. Cytochalasin D disrupted the actin cytoskeleton architecture but did not prevent the recruitment of F-actin by the bacteria. Adherent, noninvasive gonococcal Opa variants lacked the ability to mobilize F-actin. Recombinant Escherichia coli expressing the gonococcal invasion-promoting Opa of gonococcal strain MS11 (Opa50) adhered to the epithelial cells in an Opa-dependent fashion but was not internalized and did not recruit detectable amounts of F-actin. Coinfection with the E. coli recombinant strain and gonococci resulted in specific entry of the diplococci, despite the presence of large numbers of adherent E. coli cells. Together, our results indicate that Opa-mediated gonococcal entry into Chang cells resembles phagocytosis rather than macropinocytosis reported for Salmonella spp. and sequentially involves gonococcal adherence to the cell surface, Opa-dependent and cytochalasin-insensitive recruitment of F-actin, and cytochalasin D-sensitive bacterial internalization.


Infect Immun


Infection and immunity


Grassmé HU,Ireland RM,van Putten JP




Has Abstract


1996-05-01 00:00:00












  • Selection of influenza B virus recombinants and their testing in humans for attenuation and immunogenicity.

    abstract::The selection of influenza B virus recombinants from plaques in bovine kidney cell monolayers is described. Two sets of recombinants were each derived from parents of high and low virulence for humans, respectively. Recombination frequency was apparently high, and reassortment of genes made it possible to obtain atten...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Beare AS,Sherwood JE,Callow KA,Craig JW

    更新日期:1977-02-01 00:00:00

  • Identification of an iron-regulated hemin-binding outer membrane protein, HupO, in Vibrio fluvialis: effects on hemolytic activity and the oxidative stress response.

    abstract::In pathogenic bacteria, iron acquisition is important for colonization and proliferation in the host under iron-limited conditions. The ability of Vibrio spp. to acquire iron is often critical to their virulence, causing gastroenteritis or excessive watery diarrhea in humans. In the study described here, we cloned the...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ahn SH,Han JH,Lee JH,Park KJ,Kong IS

    更新日期:2005-02-01 00:00:00

  • Lipopolysaccharide-induced lethality and cytokine production in aged mice.

    abstract::This study was designed to define the lipopolysaccharide (LPS) sensitivity of aged mice in terms of lethality and cytokine production and to determine down-regulating responses of corticosterone and interleukin 10 (IL-10). The 50% lethal doses of LPS in young (6- to 7-week-old) and aged (98- to 102-week-old) mice were...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tateda K,Matsumoto T,Miyazaki S,Yamaguchi K

    更新日期:1996-03-01 00:00:00

  • Effects of immunosuppression with cyclophosphamide on acute murine cytomegalovirus infection and virus-augmented natural killer cell activity.

    abstract::The effects of cyclophosphamide (CY) treatment on acute murine cytomegalovirus (MCMV) infection were studied to explore the potential usefulness of MCMV as a means of detecting immune dysfunction and to identify host defense mechanisms important for protection against MCMV. Conditions found optimal for enhancing MCMV ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Selgrade MK,Daniels MJ,Hu PC,Miller FJ,Graham JA

    更新日期:1982-12-01 00:00:00

  • Effect of iron compounds on antibacterial function of human polymorphs and plasma.

    abstract::Human plasma was bactericidal for small numbers of Klebsiella pneumoniae, but larger numbers grew slowly over a period of 24 h. Human polymorphs in a clot of autologous plasma had a bacteriostatic effect on relatively large numbers of bacteria for up to 24 h and were much more effective than plasma alone. The bacteric...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ward CG,Hammond JS,Bullen JJ

    更新日期:1986-03-01 00:00:00

  • Requirement of histidine kinases HP0165 and HP1364 for acid resistance in Helicobacter pylori.

    abstract::In this study, we investigated a potential requirement of two-component signal transduction systems for acid resistance in Helicobacter pylori. In comparison to a wild-type strain, isogenic strains with null mutations in either HP0165 or HP1364 histidine kinases were impaired in their ability to grow at pH 5.0. The gr...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Loh JT,Cover TL

    更新日期:2006-05-01 00:00:00

  • Low-passage-associated proteins of Borrelia burgdorferi B31: characterization and molecular cloning of OspD, a surface-exposed, plasmid-encoded lipoprotein.

    abstract::Borrelia burgdorferi, the causative agent of Lyme disease, loses its ability to infect and cause disease in mammalian hosts after repeated in vitro passage. To identify proteins preferentially expressed by the low-passage strain and thus representing potential virulence factors, the polypeptide profiles of virulent, l...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Norris SJ,Carter CJ,Howell JK,Barbour AG

    更新日期:1992-11-01 00:00:00

  • Secretory leukocyte protease inhibitor interferes with uptake of lipopolysaccharide by macrophages.

    abstract::Macrophages are among the most sensitive targets of bacterial endotoxin (LPS), responding to minute amounts of LPS by releasing a battery of inflammatory mediators. Transfection of macrophages with secretory leukocyte protease inhibitor (SLPI) renders these cells refractory to LPS stimulation. Here we show that uptake...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ding A,Thieblemont N,Zhu J,Jin F,Zhang J,Wright S

    更新日期:1999-09-01 00:00:00

  • Structural similarities among malaria toxins insulin second messengers, and bacterial endotoxin.

    abstract::Malaria toxin causes hypoglycemia and induction of tumor necrosis factor. Extracts of parasitized erythrocytes which were coeluted and copurified with one of the two subtypes of mammalian insulin-mimetic inositolphosphoglycans similarly induced fibroblast proliferation in the absence of serum. In addition, induction o...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Caro HN,Sheikh NA,Taverne J,Playfair JH,Rademacher TW

    更新日期:1996-08-01 00:00:00

  • Extractability of cell wall polysaccharide from lactobacilli and streptococci by autoclaving and by dilue acid.

    abstract::Autoclaving cell wall of Streptococcus mutans Ingbritt for 15 min under the Rantz and Randall conditions released one-tenth of the total cell wall carbohydrate, whereas two-thirds was extracted after autoclaving for 180 min. The extract contained the serotype c-specific antigen but lacked the lipoteichoic acid compone...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Campbell LK,Knox KW,Wicken AJ

    更新日期:1978-12-01 00:00:00

  • Cutaneous cryptococcosis in athymic and beige-athymic mice.

    abstract::The dermotropism of Cryptococcus neoformans SLHA in congenitally athymic (nu/nu) and doubly immunodeficient beige-athymic (bg/bg-nu/nu) mice is described. Both bg/bg-nu/nu and nu/nu mice developed cutaneous cryptococcosis within 7 to 12 days following intravenous challenge with 10(4) encapsulated yeast cells. Macrosco...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Salkowski CA,Balish E

    更新日期:1991-05-01 00:00:00

  • Nonhemolytic Listeria monocytogenes mutants that are also noninvasive for mammalian cells in culture: evidence for coordinate regulation of virulence.

    abstract::We identified nonhemolytic mutants of Listeria monocytogenes that were severely deficient in their ability to invade mammalian nonprofessional phagocytes. These mutants were generated spontaneously or by means of transposon Tn916 mutagenesis. In terms of their extracellular proteins, the noninvasive mutants were defic...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kathariou S,Pine L,George V,Carlone GM,Holloway BP

    更新日期:1990-12-01 00:00:00

  • Campylobacter-Wolinella group organisms are the only oral bacteria that form arylsulfatase-active colonies on a synthetic indicator medium.

    abstract::Most oral bacteria tested formed colonies on a chemically defined medium with a chromogenic arylsulfatase substrate. Arylsulfatase activity was, however, restricted to Campylobacter-Wolinella group organisms, including Wolinella recta, a possible periodontopathogen. W. recta was the only arylsulfatase-active species a...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Wyss C

    更新日期:1989-05-01 00:00:00

  • Mechanism of fluconazole resistance in Candida albicans biofilms: phase-specific role of efflux pumps and membrane sterols.

    abstract::Candida albicans biofilms are formed through three distinct developmental phases and are associated with high fluconazole (FLU) resistance. In the present study, we used a set of isogenic Candida strains lacking one or more of the drug efflux pumps Cdr1p, Cdr2p, and Mdr1p to determine their role in FLU resistance of b...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mukherjee PK,Chandra J,Kuhn DM,Ghannoum MA

    更新日期:2003-08-01 00:00:00

  • Pathogen and host differences in bacterial adherence to human buccal epithelial cells in a northeast Brazilian community.

    abstract::The adherence of several strains of Escherichia coli to human buccal epithelial cells was studied, using cells obtained from five groups: healthy adults, healthy children, children with acute diarrhea, children with persistent diarrhea associated with cryptosporidial parasites, and children with noncryptosporidial per...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Walser BL,Newman RD,Lima AA,Guerrant RL

    更新日期:1992-11-01 00:00:00

  • Effect of vaccination with carrier protein on response to meningococcal C conjugate vaccines and value of different immunoassays as predictors of protection.

    abstract::In order to plan for the wide-scale introduction of meningococcal C conjugate (MCC) vaccine for United Kingdom children up to 18 years old, phase II trials were undertaken to investigate whether there was any interaction between MCC vaccines conjugated to tetanus toxoid (TT) or a derivative of diphtheria toxin (CRM(19...

    journal_title:Infection and immunity

    pub_type: 临床试验,杂志文章


    authors: Burrage M,Robinson A,Borrow R,Andrews N,Southern J,Findlow J,Martin S,Thornton C,Goldblatt D,Corbel M,Sesardic D,Cartwight K,Richmond P,Miller E

    更新日期:2002-09-01 00:00:00

  • Cinematographic observations of growth cycles of Chlamydia trachomatis in primary cultures of human amniotic cells.

    abstract::Time-lapse cinematography was used to study the growth cycle of Chlamydia trachomatis in primary cell cultures of human amnion. Twelve preterm and twelve term placentas were obtained within 8 h of delivery, and epithelial cells were dissociated from the amniotic membranes by trypsinization and grown in Rose chambers. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Neeper ID,Patton DL,Kuo CC

    更新日期:1990-07-01 00:00:00

  • Expression, Purification, and Biological Characterization of Babesia microti Apical Membrane Antigen 1.

    abstract::The intraerythrocytic apicomplexan Babesia microti, the primary causative agent of human babesiosis, is a major public health concern in the United States and elsewhere. Apicomplexans utilize a multiprotein complex that includes a type I membrane protein called apical membrane antigen 1 (AMA1) to invade host cells. We...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Moitra P,Zheng H,Anantharaman V,Banerjee R,Takeda K,Kozakai Y,Lepore T,Krause PJ,Aravind L,Kumar S

    更新日期:2015-10-01 00:00:00

  • New fimbrial gene cluster of S-fimbrial adhesin family.

    abstract::Fimbrial adhesins that mediate attachment to host cells are produced by most virulent Escherichia coli isolates. These virulence factors play an important role in the initial stages of bacterial colonization and also in determination of the host and tissue specificity. Isolates belonging to serotype O78 are known to c...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Babai R,Stern BE,Hacker J,Ron EZ

    更新日期:2000-10-01 00:00:00

  • Chemical and serological properties of Mycoplasma hyorhinis fractions.

    abstract::Mycoplasma hyorhinis organisms were fractionated into lipid, carbohydrate, and protein fractions by chemical and physiochemical procedures. When all fractions were tested for complement fixation and inhibition of growth inhibition activity, only the protein fractions possessed activity. Thus, it appears that the major...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Barden JA,Prescott B

    更新日期:1973-06-01 00:00:00

  • Identification of the sigB operon in Staphylococcus epidermidis: construction and characterization of a sigB deletion mutant.

    abstract::The role of the alternative sigma factor sigma(B) in Staphylococcus epidermidis was investigated by the construction, complementation, and characterization of a sigB deletion mutant. Electrophoretic analyses confirmed a profound influence of sigma(B) on the expression of exoproteins and cytoplasmic proteins. Detailed ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kies S,Otto M,Vuong C,Götz F

    更新日期:2001-12-01 00:00:00

  • Parameters affecting the enzyme-linked immunosorbent assay of immunoglobulin G antibody to a rough mutant of Salmonella minnesota.

    abstract::An enzyme-linked immunosorbent assay was developed to detect rabbit immunoglobulin G antibodies against purified lipopolysaccharide of the rough Re mutant of Salmonella minnesota. The time necessary for each step was investigated, and this resulted in a test that could be completed in 1 working day. Serial dilutions o...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bruins SC,Ingwer I,Zeckel ML,White AC

    更新日期:1978-09-01 00:00:00

  • Phase 1 and phase 2 studies of Salmonella enterica serovar paratyphi A O-specific polysaccharide-tetanus toxoid conjugates in adults, teenagers, and 2- to 4-year-old children in Vietnam.

    abstract::Salmonella enterica serovar Paratyphi A O-specific polysaccharide (O-SP) was activated with 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) and bound to tetanus toxoid (TT) with adipic acid dihydrazide as a linker (SPA-TT(1)) or directly (SPA-TT(2)). In mice, these two conjugates elicited high levels of imm...

    journal_title:Infection and immunity

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Konadu EY,Lin FY,Hó VA,Thuy NT,Van Bay P,Thanh TC,Khiem HB,Trach DD,Karpas AB,Li J,Bryla DA,Robbins JB,Szu SC

    更新日期:2000-03-01 00:00:00

  • Identification of T-cell determinants in natural immune responses to the Plasmodium falciparum apical membrane antigen (AMA-1) in an adult population exposed to malaria.

    abstract::AMA-1 of Plasmodium falciparum is a promising candidate antigen in malaria vaccine development. In this study, we have mapped the immunodominant T-cell determinants in this antigen by using synthetic peptides. From the amphipathic scores, 17 putative T-cell determinants were identified. Nine of the 17 peptides complem...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lal AA,Hughes MA,Oliveira DA,Nelson C,Bloland PB,Oloo AJ,Hawley WE,Hightower AW,Nahlen BL,Udhayakumar V

    更新日期:1996-03-01 00:00:00

  • Direct and indirect effects of soluble extracts of Schistosoma mansoni eggs on fibroblast proliferation in vitro.

    abstract::The possibility that soluble products of Schistosoma mansoni eggs might participate in the pathogenesis of hepatic fibrosis in schistosomiasis was investigated. Both crude saline extracts of eggs (soluble egg antigen [SEA]) and a partially purified SEA fraction contained activity which stimulated guinea pig and human ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Wyler DJ,Tracy JW

    更新日期:1982-10-01 00:00:00

  • Deregulated production of protective cytokines in response to Candida albicans infection in patients with chronic mucocutaneous candidiasis.

    abstract::Patients with chronic mucocutaneous candidiasis (CMC) are selectively unable to clear the yeast Candida, which results in persistent debilitating infections affecting the skin, nails, and mucous membranes. The underlying defect is unknown. Recent animal studies highlighted the importance of type 1 cytokines in protect...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lilic D,Gravenor I,Robson N,Lammas DA,Drysdale P,Calvert JE,Cant AJ,Abinun M

    更新日期:2003-10-01 00:00:00

  • Occurrence of gastric ulcers in gnotobiotic piglets colonized by Helicobacter pylori.

    abstract::Archival gastric tissues from 10 of 39 gnotobiotic piglets colonized with Helicobacter pylori 26695 contained stromal leukocytic inflammation along with ulcers and erosions. In contrast, gastric tissues from 54 archival control piglets were devoid of both inflammation and ulcers. These data confirm the hypothesis that...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Krakowka S,Eaton KA,Rings DM

    更新日期:1995-06-01 00:00:00

  • Plasmodium chabaudi-infected erythrocytes adhere to CD36 and bind to microvascular endothelial cells in an organ-specific way.

    abstract::Adherence of erythrocytes infected with Plasmodium falciparum to microvascular endothelial cells (sequestration) is considered to play an important role in parasite virulence and pathogenesis. However, the real importance of sequestration for infection and disease has never been fully assessed. The absence of an appro...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mota MM,Jarra W,Hirst E,Patnaik PK,Holder AA

    更新日期:2000-07-01 00:00:00

  • Cytotoxic T-Lymphocyte-Associated Antigen 4 (CTLA-4)- and Programmed Death 1 (PD-1)-Mediated Regulation of Monofunctional and Dual Functional CD4+ and CD8+ T-Cell Responses in a Chronic Helminth Infection.

    abstract::Chronic helminth infections are known to be associated with the modulation of antigen-specific T-cell responses. Strongyloides stercoralis infection is characterized by the downmodulation of antigen-specific Th1 and Th17 responses and the upregulation of Th2 and Th9 responses. Immune homeostasis is partially maintaine...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rajamanickam A,Munisankar S,Dolla C,Nutman TB,Babu S

    更新日期:2019-11-18 00:00:00

  • SlyA regulates function of Salmonella pathogenicity island 2 (SPI-2) and expression of SPI-2-associated genes.

    abstract::During the systemic phase of murine infection with Salmonella enterica serovar Typhimurium, bacterial virulence is correlated with the ability to grow and survive within host macrophages. Salmonella pathogenicity island 2 (SPI-2), encoding a type three secretion system, has emerged as an important contributor to Salmo...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Linehan SA,Rytkönen A,Yu XJ,Liu M,Holden DW

    更新日期:2005-07-01 00:00:00