Abstract:
:Bovine mastitis continues to be the most detrimental factor for profitable dairying. Recent research conducted within our laboratory has identified a genetic marker in the CXCR2 gene associated with mastitis susceptibility. The objective of the present study was to evaluate the migratory ability of neutrophils from cows with different CXCR2 +777 genotypes. Neutrophils isolated from peripheral blood of 30 Holstein cows were tested for in vitro migration and adhesion molecule expression. Cows with the CC or GC genotype at CXCR2 +777 showed significantly lower neutrophil migration to recombinant human interleukin-8 (rhIL-8) than cows with the GG genotype (P < 0.05). Cows with the CC genotype at CXCR2 +777 also showed decreased neutrophil migration to zymosan-activated serum compared to these same cows (P < 0.05). Decreased upregulation of CD18 expression was observed after stimulation with rhIL-8 in cows expressing the CXCR2 +777 CC genotype compared to cows expressing the GG genotype (P < 0.05). A similar trend was observed for CD11b (P < 0.10). However, no difference in CD62 downregulation was observed with respect to genotype. These results provide initial evidence for a phenotypic association between a single nucleotide polymorphism and neutrophil function in dairy cows, as well as potential insight into specific mechanisms affected in cows more susceptible to mastitis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Rambeaud M,Pighetti GMdoi
10.1128/IAI.73.8.4955-4959.2005keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
4955-9issue
8eissn
0019-9567issn
1098-5522pii
73/8/4955journal_volume
73pub_type
杂志文章abstract::Saccharomyces boulardii is a nonpathogenic yeast that protects against antibiotic-associated diarrhea and recurrent Clostridium difficile colitis. The administration of C. difficile toxoid A by gavage to S. boulardii-fed BALB/c mice caused a 1.8-fold increase in total small intestinal immunoglobulin A levels (P = 0.00...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2762-2765.2001
更新日期:2001-04-01 00:00:00
abstract::Virulent and avirulent clones of Leishmania donovani promastigotes were examined for their acid phosphatase activity. The acid phosphatase activity of whole-cell lysates of virulent clones was 1.5 to 2.0 times higher than that of avirulent clones. Pellet fractions (260,000 x g, 30 min) from sonicated promastigotes of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.11.2856-2860.1988
更新日期:1988-11-01 00:00:00
abstract::The P8 proteoglycolipid complex (P8 PGLC) is a glyconjugate expressed by Leishmania mexicana complex parasites. We previously have shown that vaccination with P8 PGLC provides protection against cutaneous leishmaniasis in susceptible BALB/c mice. However, the biological importance of this complex remains unknown. Here...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01528-07
更新日期:2008-05-01 00:00:00
abstract::Human monocytic ehrlichiosis (HME) is caused by a tick-borne obligate intracellular pathogen of the order Rickettsiales. HME disease can range from mild to a fatal, toxic shock-like syndrome, yet the mechanisms regulating pathogenesis are not well understood. We define a central role for type I interferons (alpha inte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01564-13
更新日期:2014-04-01 00:00:00
abstract::Splenic lymphocytes from cytomegalovirus-infected mice lost their in vitro proliferative responses to cytomegalovirus antigen within 3 h after in vivo treatment with antilymphocyte globulin and prednisolone. The response was inhibited when the agents were administered separately or together, and inhibition persisted t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::As an opportunistic bacterial pathogen, Pseudomonas aeruginosa mainly affects immunocompromised individuals as well as patients with cystic fibrosis. In a previous study, we showed that ExoS of P. aeruginosa, when injected into host cells through a type III secretion apparatus, functions as an effector molecule to tri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.6.3361-3370.2003
更新日期:2003-06-01 00:00:00
abstract::Cord factor (trehalose 6,6'-dimycolate[TDM] ), a mixture of 6,6'-diesters of alpha, alpha-D-trehalose with natural mycolic acids, has been described as having immunoregulatory and antitumor activities in vivo, although the relevant mechanisms of action remain unelucidated. In this work, we measured the effects of TDM ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.2.531-535.1984
更新日期:1984-02-01 00:00:00
abstract::An asd-stabilized plasmid carrying enterotoxigenic Escherichia coli cfaABCE genes was constructed and called pJGX15C-asd+. Expression of colonization factor antigen I (CFA/I) by this plasmid occurs independently of the cfaABCE positive regulator cfaR in attenuated Salmonella delta aro delta asd strain H683 and nonpath...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4933-4938.1995
更新日期:1995-12-01 00:00:00
abstract::The interaction between human neutrophils and wild-type Bordetella pertussis or mutants expressing altered lipopolysaccharide or lacking virulence factors-pertussis toxin, adenylate cyclase toxin, dermonecrotic toxin, filamentous hemagglutinin (FHA), pertactin, or BrkA-was examined. In the absence of antibodies, the w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1735-1739.2000
更新日期:2000-03-01 00:00:00
abstract::An anti-glucosyltransferase serum was prepared against a pure enzyme preparation from Streptococcus sanguis OMZ 9, which synthesized both soluble and insoluble dextran. Sera, crude gamma globulins, and antibody fractions obtained after gel filtration on a Bio-Gel P200 column were used to study enzyme-antibody interact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.619-626.1978
更新日期:1978-06-01 00:00:00
abstract::Humoral immunity does not play a prominent role during experimental cryptococcosis. However, previous studies have shown that immunoglobulin G (IgG) anti-Cryptococcus neoformans antibodies can mediate cell-dependent yeast killing in vitro. Therefore, the protective effect of a previously described monoclonal IgG1 anti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.749-752.1987
更新日期:1987-03-01 00:00:00
abstract::Human defensins play a fundamental role in the initiation of innate immune responses to some microbial pathogens. Here we show that colonic epithelial model HCT116 cells respond to Trypanosoma cruzi infection by secreting defensin α-1, which reduces infection. We also report the early effects of defensin α-1 on invasi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01459-12
更新日期:2013-11-01 00:00:00
abstract::The American opossum is the only experimental animal that regularly develops bacterial endocarditis spontaneously. There was no relation between the ability of opossums to clear bacteria from the bloodstream and the subsequent development of endocarditis. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.6.1126-1128.1974
更新日期:1974-06-01 00:00:00
abstract::Peptic ulcer disease (PUD) occurs after a long-term Helicobacter pylori infection. However, the disease can develop earlier, and rare cases have been observed in children, suggesting that these H. pylori strains may be more virulent. We used suppressive subtractive hybridization for comparative genomics between H. pyl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00123-06
更新日期:2006-07-01 00:00:00
abstract::Arthritis is a frequent and major complication of infection with Borrelia burgdorferi sensu stricto. The antigens responsible for the induction of arthritis are unknown. Here we provide direct evidence that a major surface protein, outer surface protein A (OspA), can induce arthritis. Hamsters were vaccinated with 30,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.658-663.2000
更新日期:2000-02-01 00:00:00
abstract::Gender is a significant factor in determining the susceptibility to and severity of pulmonary diseases in both humans and animals. Murine respiratory mycoplasmosis (MRM), due to Mycoplasma pulmonis infection, is an excellent animal model for evaluation of the role of various host factors on the development of acute or...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.2865-2871.2001
更新日期:2001-05-01 00:00:00
abstract::We have characterized the natural immune responses to the 19-kDa domain of merozoite surface protein 1 in individuals from an area of western Kenya in which malaria is holoendemic. We used the three known natural variant forms of the yeast-expressed recombinant 19-kDa fragment that are referred to as the E-KNG, Q-KNG,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2716-2723.1996
更新日期:1996-07-01 00:00:00
abstract::Although the suckling mouse assay is widely used for the detection of heat-stable Escherichia coli enterotoxin (ST), few data have been published concerning the reproducibility, optimal growth, and test conditions of this assay. Four strains of toxigenic E. coli known to elaborate both heat-labile enterotoxin and ST o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.1.95-99.1976
更新日期:1976-07-01 00:00:00
abstract::Borrelia burgdorferi produces potent cell-activating molecules capable of stimulating polyclonal proliferation and immunoglobulin production by murine B lymphocytes and cytokine production by a variety of cell types. These stimulatory molecules function in infected mice, resulting in elevated levels of circulating imm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.520-528.1994
更新日期:1994-02-01 00:00:00
abstract::Mice pretreated intraperitoneally with trehalose-6,6'-dimycolate (cord factor) were protected against an intraperitoneal challenge with Salmonella typhi strain Ty2 or Salmonella typhimurium strain SR 11. The nonspecific resistance to S. typhi and S. typhimurium was still detectable 7 and 14 days, respectively, after a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.5.1125-1129.1976
更新日期:1976-11-01 00:00:00
abstract::A major obstacle to studying the functions of particular gene products in the mouse-tick infectious cycle of Borrelia burgdorferi has been an inability to knock out genes in pathogenic strains. Here, we investigated conditions for site-directed mutagenesis in B31 MI, the low-passage-number, infectious B. burgdorferi s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.2139-2150.2002
更新日期:2002-04-01 00:00:00
abstract::Legionella pneumophila is a gram-negative facultative intracellular parasite of macrophages. Although L. pneumophila is the causative agent of a severe pneumonia known as Legionnaires' disease, it is likely that most infections caused by this organism are cleared by the host innate immune system. It is predicted that ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02049-05
更新日期:2006-06-01 00:00:00
abstract::Q fever is a zoonotic disease of worldwide significance caused by the obligate intracellular bacterium Coxiella burnetii. Humans with Q fever may experience an acute flu-like illness and pneumonia and/or chronic hepatitis or endocarditis. Various markers demonstrate significant phylogenetic separation between and clus...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00851-09
更新日期:2009-12-01 00:00:00
abstract::Infection of epithelial cells by two biovars of Chlamydia trachomatis results in the tyrosine phosphorylation of several host proteins. The most prominent change in host protein tyrosine phosphorylation involves a complex of proteins with molecular masses of 75 to 85 kDa (pp75/85) and 100 kDa (pp100). The C. trachomat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.5301-5308.1997
更新日期:1997-12-01 00:00:00
abstract::Streptolysin O (SLO) and streptolysin S (SLS) are potent cytolytic toxins produced by almost all clinical isolates of group A streptococci (GAS). Allele-replacement mutagenesis was used to construct nonpolar (in-frame) deletion mutations in the slo and sagB genes of the serotype M5 GAS strain Manfredo, producing isoge...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.3857-3865.2003
更新日期:2003-07-01 00:00:00
abstract::The virulence and fitness in vivo of the major human pathogen Staphylococcus aureus are associated with a cell-to-cell signaling mechanism known as quorum sensing (QS). QS coordinates the production of virulence factors via the production and sensing of autoinducing peptide (AIP) signal molecules by the agr locus. Her...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01216-13
更新日期:2014-03-01 00:00:00
abstract::The theoretic basis for developing conjugate vaccines, to induce immunoglobulin G (IgG) lipopolysaccharide (LPS) antibodies for the prevention of shigellosis, has been described (J. B. Robbins, C.-Y. Chu, and R. Schneerson, Clin. Infect. Dis. 15:346-361, 1992). The O-specific polysaccharides (O-SPs) of Shigella dysent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3678-3687.1993
更新日期:1993-09-01 00:00:00
abstract::Since 1984, it has been known that spotted fever group rickettsiosis exists in Japan. We isolated three strains of the causative rickettsiae, designated Katayama, Misaka, and Abe, from patients with the disease and studied the characteristics of the isolates. Nude mice and cyclophosphamide-treated mice died after infe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.887-892.1990
更新日期:1990-04-01 00:00:00
abstract::The lungs of conventional rats with chronic pneumonia contained Streptobacillus moniliformis and Mycoplasma pulmonis. These organisms singly and in combination failed to produce lung disease when inoculated into specific pathogen-free rats. On the other hand, diseased lung homogenate not containing cultivable organism...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.1.83-91.1972
更新日期:1972-07-01 00:00:00
abstract::Bordetella pertussis 165-9K/129G, which produces a nontoxic form of pertussis toxin (PT), was used to prepare a whole-cell diphtheria-tetanus-pertussis (DTP) vaccine. The in vivo potency and the serological response induced by this vaccine were comparable to those of the conventional DTP vaccine which contains active ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.1150-1155.1992
更新日期:1992-03-01 00:00:00