Abstract:
:We have characterized the natural immune responses to the 19-kDa domain of merozoite surface protein 1 in individuals from an area of western Kenya in which malaria is holoendemic. We used the three known natural variant forms of the yeast-expressed recombinant 19-kDa fragment that are referred to as the E-KNG, Q-KNG, and E-TSR antigens. T-cell proliferative responses in individuals older than 15 years and the profile of immunoglobulin G (IgG) antibody isotypes in individuals from 2 to 74 years old were determined. Positive proliferative responses to the Q-KNG antigen were observed for 54% of the individuals, and 37 and 35% of the individuals responded to the E-KNG and E-TSR constructs, respectively. Considerable heterogeneity in the T-cell proliferative responses to these three variant antigens was observed in different individuals, suggesting that the 19-kDa antigen may contain variant-specific T epitopes. Among responses of the different isotypes of the IgG antibody, IgG1 and IgG3 isotype responses were predominant, and the prevalence and levels of the responses increased with age. We also found that a higher level of IgG1 antibody response correlated with lower parasite density among young age groups, suggesting that IgG1 antibody response may play a role in protection against malaria. However, there was no correlation between the IgG3 antibody level and protection. Furthermore, we observed that although the natural antibodies cross-reacted with all three variant 19-kDa antigens, IgG3 antibodies in 12 plasma samples recognized only the E-KNG and Q-KNG constructs and not the E-TSR antigen. This result suggests that the fine specificity of IgG3 antibodies differentiates among variant-specific natural B-cell determinants in the second epidermal growth factor domain (KNG and TSR) of the antigen.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Shi YP,Sayed U,Qari SH,Roberts JM,Udhayakumar V,Oloo AJ,Hawley WA,Kaslow DC,Nahlen BL,Lal AAdoi
10.1128/IAI.64.7.2716-2723.1996subject
Has Abstractpub_date
1996-07-01 00:00:00pages
2716-23issue
7eissn
0019-9567issn
1098-5522journal_volume
64pub_type
杂志文章abstract::The hemolytic and sphingomyelinase C activities of supernatants of cultures of Leptospira interrogans serovar pomona tended to copurify when isoelectric fractionation was carried out. Both activities focused primarily at pH 8.1. Considered in conjunction with other circumstantial evidence, the results led to the concl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.1.262-264.1986
更新日期:1986-10-01 00:00:00
abstract::A 60-kilodalton (kDa) immunodominant antigen of Legionella pneumophila is a heat shock protein (HSP) of the GroEL class of HSPs. The gene (htpB) coding the 60-kDa protein was localized to a 3.2-kilobase DNA fragment of L. pneumophila cloned into pUC19 (pSH16) (P. S. Hoffman, C. A. Butler, and F. D. Quinn, Infect. Immu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.10.3380-3387.1990
更新日期:1990-10-01 00:00:00
abstract::The influence of D-alanine and carbohydrate substitution of lipoteichoic acids (LTAs) on the binding of antibody directed to the polyglycerol phosphate (PGP) portion was found to be at least partially dependent upon the mode of presentation of the antigen. There were greater differences in binding of anti-PGP immunogl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.2.549-555.1983
更新日期:1983-08-01 00:00:00
abstract::Peripheral leukocytes from 16 Thai children with dengue hemorrhagic fever were examined to determine the leukocyte composition on the day of presentation and on convalescent days 15 and 30. Mononuclear cells were isolated each time, and the concentrations of T, B, Fc receptor-bearing, and "null" cells were determined....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::The expression of the Shigella flexneri chromosomal aerobactin genes during growth of the bacterium within tissue culture cells was assayed. During intracellular growth, aerobactin promoter activity was repressed relative to the level observed in bacteria grown extracellularly, even when the bacteria had been starved ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.818-821.1997
更新日期:1997-02-01 00:00:00
abstract::Haemophilus influenzae type b (Hib) is a significant pathogen for young children, and three Hib vaccines (named PRP-OMPC, HbOC, and PRP-T) are currently available for young children. Extensive studies of anti-Hib polysaccharide (PS) antibodies (Abs) have shown that the V regions of Abs against the Hib PS comprise a VH...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.11.4219-4223.1995
更新日期:1995-11-01 00:00:00
abstract::Scrub typhus immunogens were prepared by exposing infected yolk sac suspensions of Rickettsia tsutsugamushi to various doses of gamma radiation. Mouse lethality was abolished at doses greater than 200 krads, whereas immunogenicity of the suspensions, as shown by mouse protection tests, was diminished relatively little...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.124-131.1977
更新日期:1977-01-01 00:00:00
abstract::Recent reports have suggested that oral vaccination of mice against Helicobacter pylori is dependent on a Th1-mediated immune response. However, oral vaccination in mice neither induces sterilizing immunity nor leads to complete protection from disease. Therefore, in this study we investigated whether a systemic subcu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.1029-1035.2004
更新日期:2004-02-01 00:00:00
abstract::Serine proteases and serine protease homologs form the second largest gene family in the Drosophila melanogaster genome. Certain genes in the Jonah multigene family encoding serine proteases have been implicated in the fly antiviral immune response. Here, we report the involvement of Jonah66Ci in the Drosophila immune...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00094-19
更新日期:2019-08-21 00:00:00
abstract::K99 prepared by acid precipitation hemagglutinated guinea pig erythrocytes, whereas K99 prepared by chromatography on diethylaminoethyl-Sephadex did not. K99 purified by either procedure hemagglutinated horse erythrocytes. K99 prepared by acid precipitation contained a second antigen not presnet in the K99 prepared by...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.2.555-559.1978
更新日期:1978-11-01 00:00:00
abstract::We report the cloning, molecular characterization, and purification of functionally active recombinant glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the human bloodfluke Schistosoma japonicum. The GAPDH homolog from the related species Schistosoma mansoni has shown correlation of antibody titer to resistance t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4716-4723.1993
更新日期:1993-11-01 00:00:00
abstract::Hot phenol-water extraction of smooth Brucella abortus and B. melitensis cells yielded a toxic fraction which was recovered from the phenol phase (fraction 5). Chemically, fractions 5 from both Brucella species were lipid-carbohydrate-protein-2 keto-3-deoxyoctulosonic acid complexes which were stable to heat and resis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1970-02-01 00:00:00
abstract::We have previously described a new system for the delivery of recombinant antigens in live Salmonella vaccines as genetic fusions to the C terminus of fragment C of tetanus toxin (TetC) driven by the anaerobically inducible nirB promoter. It has been reported that preimmunization with tetanus toxoid (TT) can suppress ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2564-2569.1995
更新日期:1995-07-01 00:00:00
abstract::The theoretic basis for developing conjugate vaccines, to induce immunoglobulin G (IgG) lipopolysaccharide (LPS) antibodies for the prevention of shigellosis, has been described (J. B. Robbins, C.-Y. Chu, and R. Schneerson, Clin. Infect. Dis. 15:346-361, 1992). The O-specific polysaccharides (O-SPs) of Shigella dysent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3678-3687.1993
更新日期:1993-09-01 00:00:00
abstract::The enteric pathogen Shigella is one of the leading causes of moderate-to-severe diarrhea and death in young children in developing countries. Transformed cell lines and animal models have been widely used to study Shigella pathogenesis. In addition to altered physiology, transformed cell lines are composed of a singl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00740-18
更新日期:2019-03-25 00:00:00
abstract::The gastric spirillum Helicobacter felis, originally isolated from the cat stomach, colonizes the stomachs of germfree rats. Studies were designed to examine the pathological and serological responses of germfree rats inoculated orally with H. felis. At 2 weeks postinoculation, the gastric mucosa of germfree rats had ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.785-791.1991
更新日期:1991-03-01 00:00:00
abstract::During the course of infection of rabbits with vaccinia virus, macrophages obtained from the peritoneal cavity develop bactericidal activity and the replication of vaccinia virus becomes restricted in these cells. The abortive replication of vaccinia virus in the activated macrophages was characterized in the present ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.328-338.1979
更新日期:1979-10-01 00:00:00
abstract::Citrobacter rodentium, an attaching-effacing bacterial pathogen, establishes an acute infection of the murine colonic epithelium and induces a mild colitis in immunocompetent mice. This study describes the role of T-cell subsets and B lymphocytes in immunity to C. rodentium. C57Bl/6 mice orally infected with C. rodent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5077-5086.2003
更新日期:2003-09-01 00:00:00
abstract::A series of experiments was conducted to study the influence of embryonal bursectomy (EBX) on the early and late pathogenesis of Marek's disease (MD). The early lytic infection in the lymphoid organs normally associated with oncogenic MD virus infection in intact chickens was not seen in EBX chickens. Therefore, the d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.199-207.1981
更新日期:1981-01-01 00:00:00
abstract::After aerosol deposition of Serratia marcescens in mice, initial delayed pulmonary clearance was followed between 4 and 24 hr later by progressive elimination. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.1.187-188.1971
更新日期:1971-01-01 00:00:00
abstract::Invasive aspergillosis is a serious fungal infection caused by the proliferation and invasion of Aspergillus hyphae in tissue. Neutrophils (PMNs) are the most important line of defense against Aspergillus hyphae. To investigate the role of granulocyte colony-stimulating factor (G-CSF) and gamma interferon (IFN-gamma) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.4.1185-1193.1993
更新日期:1993-04-01 00:00:00
abstract::We show in this study that toxin production in Clostridium difficile is altered in cells which can no longer form flagellar filaments. The impact of inactivation of fliC, CD0240, fliF, fliG, fliM, and flhB-fliR flagellar genes upon toxin levels in culture supernatants was assessed using cell-based cytotoxicity assay, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00224-12
更新日期:2012-10-01 00:00:00
abstract::Rabbits are not usually susceptible to intestinal Shigella infection without extensive pretreatment, including starvation and administration of antimicrobial, antimotility, and toxic agents (carbon tetrachloride). Most animals infected this way die rapidly and do not always develop colonic lesions and signs of dysente...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.11.4350-4357.1995
更新日期:1995-11-01 00:00:00
abstract::Bacterial superantigens (SAgs) have been implicated in inflammatory disease, and SAg-treated mice have increased jejunal T cells. Here we show that T84 cells (a human epithelial cell line) display increased MCP-1 and RANTES mRNA expression and protein production in response to conditioned medium from Staphylococcus au...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.6198-6202.1999
更新日期:1999-11-01 00:00:00
abstract::Three monoclonal antibodies (MAbs), E4, L1-4, and L1-24, to the major outer membrane protein (MOMP) of Chlamydia trachomatis were identified that neutralized in vitro the infectivity of members of the B- and C-related complex as well as the mouse pneumonitis strain. MAbs L1-4, L1-24, and E4 gave a strong signal in an ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.11.4147-4153.1991
更新日期:1991-11-01 00:00:00
abstract::We investigated the ability of two overlapping fragments of staphylococcal enterotoxin B (SEB), which encompass the whole toxin, to induce protection and also examined if passive transfer of chicken anti-SEB antibodies raised against the holotoxin could protect rhesus monkeys against aerosolized SEB. Although both fra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2278-2281.2002
更新日期:2002-05-01 00:00:00
abstract::Urease activity has recently been shown to be an important virulence determinant for Helicobacter pylori, allowing it to survive the low pH of the stomach during colonization. Experimental murine infection with Helicobacter felis is now being used as a model for H. pylori infection to study the effects of vaccines, an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.3.793-798.1994
更新日期:1994-03-01 00:00:00
abstract::Lewis (Le) antigens have been implicated in the pathogenesis of atrophic gastritis and gastric cancer in the setting of Helicobacter pylori infection, and H. pylori-induced anti-Le antibodies have been described that cross-react with the gastric mucosa of both mice and humans. The aim of this study was to examine the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.4774-4781.2001
更新日期:2001-08-01 00:00:00
abstract::Borrelia burgdorferi elicits a potent cytokine response through activation of multiple signaling receptors on innate immune cells. Spirochetal lipoproteins initiate expression of NF-κB-dependent cytokines primarily via TLR2, whereas type I interferon (IFN) production is induced through the endosomal receptors TLR7 and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01617-14
更新日期:2014-06-01 00:00:00
abstract::The mechanism of complement-mediated killing of seven serum-sensitive Pseudomonas aeruginosa strains was examined. All seven strains were sensitive to the bactericidal activity of 20% pooled normal human serum (PNHS) containing magnesium EGTA, which blocks the classical complement pathway (CCP), or 20% PNHS preheated ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.3.632-639.1988
更新日期:1988-03-01 00:00:00