Abstract:
:Salmonella enterica serovar Typhimurium, similar to various facultative intracellular pathogens, has been shown to respond to the hostile conditions inside macrophages of the host organism by inducing stress proteins, such as DnaK. DnaK forms a chaperone machinery with the cochaperones DnaJ and GrpE. To elucidate the role of the DnaK chaperone machinery in the pathogenesis of S. enterica serovar Typhimurium, we first constructed an insertional mutation in the dnaK-dnaJ operon of pathogenic strain chi3306. The DnaK/DnaJ-depleted mutant was temperature sensitive for growth, that is, nonviable above 39 degrees C. We then isolated a spontaneously occurring revertant of the dnaK-dnaJ-disrupted mutant at 39 degrees C and used it for infection of mice. The mutant lost the ability to cause a lethal systemic disease in mice. The impaired ability for virulence was restored when a functional copy of the dnaK-dnaJ operon was provided, suggesting that the DnaK/DnaJ chaperone machinery is required by Salmonella for the systemic infection of mice. This result also indicates that with respect to the DnaK/DnaJ chaperone machinery, the cellular requirements for growth at a high temperature are not identical to the cellular requirements for the pathogenesis of Salmonella. Macrophage survival assays revealed that the DnaK/DnaJ-depleted mutant could not survive or proliferate at all within macrophages. Of further interest are the findings that the mutant could neither invade cultured epithelial cells nor secrete any of the invasion proteins encoded within Salmonella pathogenicity island 1. This is the first time that the DnaK/DnaJ chaperone machinery has been shown to be involved in bacterial invasion of epithelial cells.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Takaya A,Tomoyasu T,Matsui H,Yamamoto Tdoi
10.1128/iai.72.3.1364-1373.2004keywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
1364-73issue
3eissn
0019-9567issn
1098-5522journal_volume
72pub_type
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更新日期:1970-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.4.834-843.1974
更新日期:1974-10-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1996-07-01 00:00:00
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更新日期:1983-01-01 00:00:00
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更新日期:2021-01-19 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1982-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1995-06-01 00:00:00