Detection in antisera of antibodies that cross-react with herpes simplex virus type 1 glycoprotein gC.

Abstract:

:Herpes simplex virus type 1 (HSV-1) glycoprotein gC was purified by affinity chromatography with an immunosorbent column containing monoclonal antibody to HSV-1 gC, and its reactivity with rabbit antisera was measured by enzyme-linked immunosorbent assay and sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of radioimmunoprecipitates. Positive reactions were detected between HSV-1 gC and rabbit hyperimmune antisera to both HSV-1 and HSV-2. Electrophoretic analysis also revealed reactivity between the rabbit antisera and peptides of HSV-1 gC generated by partial digestion with trypsin. These findings indicate that HSV-1 gC has one or more cross-reactive or type-common determinants that are readily detected, and therefore, the presence of antibodies reacting with HSV-1 gC in sera may not necessarily be indicative of an earlier infection with HSV-1.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Zweig M,Heilman CJ Jr,Bladen SV,Showalter SD,Hampar B

doi

10.1128/IAI.41.2.482-487.1983

subject

Has Abstract

pub_date

1983-08-01 00:00:00

pages

482-7

issue

2

eissn

0019-9567

issn

1098-5522

journal_volume

41

pub_type

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