Abstract:
:Antigens were extracted from a virulent isolate of Pasteurella multocida (serotype 3, 12, 15:D) with potassium thiocyanate, and a vaccine was prepared. Pasteurella-free rabbits were vaccinated intranasally and intraconjuctivally twice with a 2-week interval and challenged intranasally with the homologous P. multocida serotype 2 weeks after the second vaccination. The vaccinated rabbits produced serum immunoglobulin G and nasal mucosal immunoglobulin A against P. multocida. The vaccine protected the challenged rabbits against clinical disease and death; however, otitis media was not prevented, and microscopic inflammatory lesions were occasionally noted in the lungs and nasal turbinates. In contrast, nonvaccinated, challenged rabbits became febrile, dyspnic, depressed, and anorectic, and five of six died within 4 days of challenge with severe lesions including pneumonia, pleuritis, otitis media, and bacteremia. The vaccine prevented death and colonization of challenge organisms in the blood and lung, but did not prevent colonization of the middle ear. The vaccine alone did not cause clinical disease or gross lesions, but did produce microscopic pulmonary inflammatory lesions.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Ringler DH,Peter GK,Chrisp CE,Keren DFdoi
10.1128/IAI.49.3.498-504.1985subject
Has Abstractpub_date
1985-09-01 00:00:00pages
498-504issue
3eissn
0019-9567issn
1098-5522journal_volume
49pub_type
杂志文章abstract::Vibrio cholerae is a monoflagellated gram-negative bacterium that causes the severe diarrheal disease cholera. In contrast to Salmonella enterica serovar Typhimurium infection, which is accompanied by both acute diarrhea and high-level inflammation, V. cholerae infection is largely noninflammatory in human hosts. Bact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00736-07
更新日期:2008-03-01 00:00:00
abstract::A gene encoding the cytotoxic enterotoxin (Act) from Aeromonas hydrophila was hyperexpressed with the pET, pTRX, and pGEX vector systems. Maximum toxin yield was obtained with the pTRX vector. Approximately 40 to 60% of Act was in a soluble form with the pTRX and pET vector systems. The toxin protein was purified to h...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4299-4308.1997
更新日期:1997-10-01 00:00:00
abstract::A number of studies have substantiated the pivotal role of innate defense mechanisms in protection against invasive aspergillosis. However, experiments demonstrating increased resistance to lethal intravenous (i.v.) infection with Aspergillus fumigatus conidia in cortisone-treated or untreated mice preinfected with a ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3791-3802.1993
更新日期:1993-09-01 00:00:00
abstract::An experimental model of coxsackievirus B3 infection in newborn mice was utilized to examine the protective role of antiviral antibodies and an interferon inducer, polyinosinic acid-polycytidylic acid [poly(I:C)]. Subcutaneous administration to the infected mice of specific antiviral antibodies resulted in significant...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.2.720-727.1982
更新日期:1982-08-01 00:00:00
abstract::Subtilase cytotoxin (SubAB) was first isolated from a Shiga toxigenic Escherichia coli (STEC) strain that was responsible for an outbreak of hemolytic-uremic syndrome and is the prototype of a new family of AB(5) cytotoxins. SubAB is a subtilase-like serine protease, and upon uptake by host cells, it is trafficked to ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00801-10
更新日期:2010-11-01 00:00:00
abstract::The glycolipid haptens of Mycoplasma pneumoniae became immunogenic when bound to membrane proteins of Acholeplasma laidlawii by reaggregation. This process consisted of the solubilization of lipid-depleted A. laidlawii membranes and M. pneumoniae glycolipids in 20 mm sodium dodecyl sulfate and dialysis of the mixed so...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.3.420-423.1971
更新日期:1971-03-01 00:00:00
abstract::Pseudomonas aeruginosa is one of the most frequently encountered bacterial pathogens in patients with chronic pulmonary infections, including cystic fibrosis and diffuse panbronchiolitis. Bronchus-associated lymphoid tissue (BALT), noted frequently in patients with cystic fibrosis and diffuse panbronchiolitis, is cons...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1514-1520.1991
更新日期:1991-04-01 00:00:00
abstract::Endotoxin-treated mice exhibit a rapid rise in the level of the serum amyloid A (SAA) protein, but this effect is not observed in endotoxin-resistant C3H/HeJ mice. To evaluate the role of lymphoid cells in the production of SAA protein, C3H/HeJ mice were adoptively transfused with endotoxin-sensitive (C3H/HeN) bone ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.1.181-183.1979
更新日期:1979-01-01 00:00:00
abstract::Bacillus cereus causes exotoxin-mediated diarrheal food poisoning. Hemolysin BL (HBL) is a well-characterized B. cereus toxin composed of three components (B, L1, and L2) that together possess hemolytic, cytotoxic, dermonecrotic, and vascular permeability activities. Here, we show that HBL causes fluid accumulation in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.11.4423-4428.1995
更新日期:1995-11-01 00:00:00
abstract::Cell-mediated immune (CMI) responses and tumor necrosis factor alpha (TNF-alpha) have been shown to be essential in acquired protection against Cryptococcus neoformans. Induction of a protective anticryptococcal CMI response includes increases in dendritic cells (DC) and activated CD4(+) T cells in draining lymph node...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.68-74.2003
更新日期:2003-01-01 00:00:00
abstract::Helicobacter pylori has been directly linked with active chronic gastritis, peptic ulceration, and gastric adenocarcinoma in humans. Although a substantial portion of the human population is colonized with H. pylori, the patterns of transmission of the organism remain in doubt, and reservoir hosts have not been identi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.6.2367-2374.1994
更新日期:1994-06-01 00:00:00
abstract::A major obstacle to studying the functions of particular gene products in the mouse-tick infectious cycle of Borrelia burgdorferi has been an inability to knock out genes in pathogenic strains. Here, we investigated conditions for site-directed mutagenesis in B31 MI, the low-passage-number, infectious B. burgdorferi s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.2139-2150.2002
更新日期:2002-04-01 00:00:00
abstract::When immunocompetent mice are inoculated with Borrelia burgdorferi, they develop acute arthritis and carditis that undergo spontaneous regression despite the persistence of infection. Specific T- and/or B-cell immunity appears to be necessary for resolution of disease manifestations. Humoral immune responses to B. bur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.2104-2107.1993
更新日期:1993-05-01 00:00:00
abstract::Streptococcus mutans, a major oral pathogen responsible for dental caries formation, possesses a variety of mechanisms for survival in the human oral cavity, where the conditions of the external environment are diverse and in a constant state of flux. The formation of biofilms, survival under conditions of acidic pH, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01627-07
更新日期:2008-07-01 00:00:00
abstract::Strains of type III group B streptococci isolated from patients with neonatal sepsis are generally resistant to complement-mediated phagocytic killing in the absence of specific antibody. It has been suggested that the resistance of type III group B streptococci to phagocytosis results from inhibition of alternative-c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.10.3986-3993.1992
更新日期:1992-10-01 00:00:00
abstract::The ability of human clinical isolates of Yersinia enterocolitica to interact with HEp-2 cells was found to vary considerably between the serotypes O:3, O:8, and O:9. Although all three serotypes adhered initially to the cell surface, regardless of incubation temperature of the bacterial inoculum or presence of the 40...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.85-89.1986
更新日期:1986-04-01 00:00:00
abstract::An antigen recognized by monoclonal antibody specific for Bacteroides gingivalis was purified in the presence of 0.5% (wt/vol) beta-octyl-glucoside by immunoadsorbent column chromatography. The purified antigen was homogeneous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.2.366-371.1986
更新日期:1986-08-01 00:00:00
abstract::Group A streptococci of M protein type 5 have been epidemiologically related to acute rheumatic fever in a number of reported outbreaks. Preliminary bacteriological evidence suggests that M5 may be an important "rheumatogenic" type in Santiago, Chile. To assess further the relationship of this streptococcal serotype t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.573-579.1982
更新日期:1982-11-01 00:00:00
abstract::Apical membrane antigen 1 (AMA1) is expressed on the surfaces of Plasmodium falciparum merozoites and is thought to play an important role in the invasion of erythrocytes by malaria parasites. To select for peptides that mimic conformational B-cell epitopes on AMA1, we screened a phage display library of >10(8) indivi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.1126-1134.2004
更新日期:2004-02-01 00:00:00
abstract::Intracellular survival of Chlamydia psittaci is in part dependent on the ability of the organism to thwart phagolysosome formation. Circumvention of phagolysosome fusion could be either localized to chlamydia-laden vacuoles or generalized to all phagosomes in the host cell. To determine which of these modes is in oper...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.889-896.1981
更新日期:1981-05-01 00:00:00
abstract::Pseudomonas aeruginosa is a leading cause of hospital-acquired pneumonia and chronic lung infections in cystic fibrosis patients. Iron is essential for bacterial growth, and P. aeruginosa expresses multiple iron uptake systems, whose role in lung infection deserves further investigation. P. aeruginosa Fe(3+) uptake sy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00098-16
更新日期:2016-07-21 00:00:00
abstract::Protamines (salmine prepared from sperm DNA of salmon and clupeine from herring sperm), which are basic peptides rich in arginine, were found to inhibit the proteolytic activity of arginine-specific cysteine protease (RC-protease) from Porphyromonas gingivalis. Lineweaver-Burk plot analysis revealed that the protamine...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4917-4920.1999
更新日期:1999-09-01 00:00:00
abstract::The complete nucleotide sequence of a 612-base-pair DNA fragment containing the gene for the major fimbrial subunit of CS3 of enterotoxigenic Escherichia coli is presented. A possible promoter region, a ribosome-binding site, and two potential signal peptidase cleavage sites are indicated. Unlike the best-studied fimb...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3297-3300.1988
更新日期:1988-12-01 00:00:00
abstract::Contributions of the alternative sigma factor sigmaB to Listeria monocytogenes infection were investigated using strains bearing null mutations in sigB, prfA, or inlA or in selected inlA or prfA promoter regions. The DeltaP4inlA strain, which has a deletion in the sigmaB-dependent P4inlA promoter, and the DeltasigB st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.876-886.2006
更新日期:2006-02-01 00:00:00
abstract::Pseudomallei group Burkholderia species are facultative intracellular parasites that spread efficiently from cell to cell by a mechanism involving the fusion of adjacent cell membranes. Intercellular fusion requires the function of the cluster 5 type VI secretion system (T6SS-5) and its associated valine-glycine repea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01367-13
更新日期:2014-04-01 00:00:00
abstract::Helicobacter pylori infection of the stomach causes an active immune response that includes stimulation of inducible nitric oxide (NO) synthase (iNOS) expression. Although NO can kill H. pylori, the bacterium persists indefinitely, suggesting that NO production is inadequate. We determined if the NO derived from iNOS ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00578-07
更新日期:2007-09-01 00:00:00
abstract::Two virulence factors of Listeria monocytogenes, listeriolysin O (LLO) and phosphatidylinositol-specific phospholipase C (PI-PLC), mediate escape of this pathogen from the phagocytic vacuole of macrophages, thereby allowing the bacterium access to the host cell cytosol for growth and spread to neighboring cells. We ch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6639-6646.2005
更新日期:2005-10-01 00:00:00
abstract::Temperate bacteriophage can transfer toxin-encoding genes between bacteria, often resulting in acquired pathogenicity. However, little is known regarding the effects of the eukaryotic host on the phage-pathogen interaction. Using Streptococcus pyogenes as a model, we demonstrate, both in vitro and in vivo, that the eu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.3782-3786.2003
更新日期:2003-07-01 00:00:00
abstract::A recent study found that group A Streptococcus (GAS) expresses a cell surface protein with similarity to human collagen (S. Lukomski, K. Nakashima, I. Abdi, V. J. Cipriano, R. M. Ireland, S. R. Reid, G. G. Adams, and J. M. Musser, Infect. Immun. 68:6542-6553, 2000). This streptococcal collagen-like protein (Scl) cont...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1729-1738.2001
更新日期:2001-03-01 00:00:00
abstract::By crossed immunoelectrophoresis and Western blotting (immunoblotting), it was shown that Borrelia burgdorferi expresses the 60-kilodalton Common Antigen (CA) that is cross-reactive with an equivalent antigen in a wide range of remotely related bacteria. B. burgdorferi CA is strongly immunogenic. A B. burgdorferi geno...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.2047-2053.1988
更新日期:1988-08-01 00:00:00