Abstract:
:The Pseudomonas aeruginosa quorum-sensing systems, las and rhl, control the production of numerous virulence factors. In this study, we have used the burned-mouse model to examine the contribution of quorum-sensing systems to the pathogenesis of P. aeruginosa infections in burn wounds. Different quorum-sensing mutants of P. aeruginosa PAO1 that were defective in the lasR, lasI, or rhlI gene or both the lasI and rhlI genes were utilized. The following parameters of the P. aeruginosa infection were examined: (i) lethality to the burned mouse, (ii) dissemination of the P. aeruginosa strain within the body of the infected mouse (by determining the numbers of CFU of P. aeruginosa within the liver and spleen), and (iii) spread of the P. aeruginosa strain within the burned skin (by determining the numbers of CFU of P. aeruginosa at the inoculation site and at a site about 15 mm from the inoculation site [distant site]). In comparison with that of PAO1, the in vivo virulence of lasI, lasR, and rhlI mutants was significantly reduced. However, the most significant reduction in in vivo virulence was seen with the lasI rhlI mutant. The numbers of CFU that were recovered from the livers, spleens, and skin of mice infected with different mutants were significantly lower than those of PAO1. At 8 and 16 h post burn infection, comparable numbers of CFU of PAO1 and lasI and rhlI mutants were obtained from both the inoculation and distant sites of the burned skin of infected mice. In contrast, CFU of the lasR mutant and the lasI rhlI double mutant were recovered only from the inoculation site of infected mice at 8 and 16 h post burn infection. The ability of a plasmid carrying either the lasI or rhlI gene or the lasI and rhlI genes to complement the defect of the lasI rhlI double mutant was also examined. The presence of any of these plasmids within the lasI rhlI double mutant significantly enhanced its in vivo virulence, as well as its ability to spread within the burned skin. These results suggest that the quorum-sensing systems play an important role in the horizontal spread of P. aeruginosa within burned skin and in the dissemination of P. aeruginosa within the bodies of burned-and-infected mice and contributed to the overall virulence of P. aeruginosa in this animal model.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Rumbaugh KP,Griswold JA,Iglewski BH,Hamood ANdoi
10.1128/IAI.67.11.5854-5862.1999keywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
5854-62issue
11eissn
0019-9567issn
1098-5522journal_volume
67pub_type
杂志文章abstract::Human monocytic ehrlichiosis (HME) is caused by a tick-borne obligate intracellular pathogen of the order Rickettsiales. HME disease can range from mild to a fatal, toxic shock-like syndrome, yet the mechanisms regulating pathogenesis are not well understood. We define a central role for type I interferons (alpha inte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01564-13
更新日期:2014-04-01 00:00:00
abstract::Affinity-purified rabbit polyclonal (PAB96-1) and mouse monoclonal (1G9-1C2) antibodies to synthetic H-DDDDDDD-OH, an antimicrobial anionic peptide (AP) originally isolated from ovine pulmonary surfactant, were prepared and used to assess the concentrations of AP-like molecules in human respiratory tract samples. In b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.4256-4259.1999
更新日期:1999-08-01 00:00:00
abstract::Ten micrograms of trehalose-6,6' -dimycolate (cord factor) injected into the footpads of mice increased the antibody response to sheep red blood cells (SRBC) subsequently injected into the same sites. There is a relationship between the antibody response and the cellular reaction induced locally and in the draining ly...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.3.256-263.1971
更新日期:1971-09-01 00:00:00
abstract::Brucella species can cause brucellosis, a zoonotic disease that causes serious livestock economic losses and represents a public health threat. The mechanism of virulence of Brucella spp. is not yet fully understood. Therefore, it is crucial to identify new molecules that serve as virulence factors to better understan...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02790-14
更新日期:2015-04-01 00:00:00
abstract::Lung dendritic cells were identified by immunohistochemistry in lung tissue sections from C57BL/6 mice. Following isolation from the lungs using CD11c magnetic beads, the flow cytometric analysis of I-A(b+) and CD11c(+) cells indicated a mixed population of dendritic cells at different stages of maturation, with most ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.1127-1133.2001
更新日期:2001-02-01 00:00:00
abstract::An immunogenic aminopeptidase was purified from Brucella melitensis strain VTRM1. The purification procedure consisted of ammonium sulfate fractionation and three chromatographic steps. This procedure resulted in a yield of 29% and a 144-fold increase in specific activity. The aminopeptidase appeared to be a monomeric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5238-5244.2003
更新日期:2003-09-01 00:00:00
abstract::Detoxified mutants of the Escherichia coli heat-labile toxin (LT) act as mucosal adjuvants to intranasally presented coadministered antigens. Here, we compare the adjuvant activity of a panel of detoxified derivatives of LT, using both intranasal (i.n.) and oral (p.o.) routes of administration. The mutants used as adj...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4400-4406.1999
更新日期:1999-09-01 00:00:00
abstract::Autoclaving cell wall of Streptococcus mutans Ingbritt for 15 min under the Rantz and Randall conditions released one-tenth of the total cell wall carbohydrate, whereas two-thirds was extracted after autoclaving for 180 min. The extract contained the serotype c-specific antigen but lacked the lipoteichoic acid compone...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.842-851.1978
更新日期:1978-12-01 00:00:00
abstract::Neonatal piglets have been used as models to study human campylobacteriosis and helicobacteriosis. The purpose of this study was to determine the relative pathogenicities, on the basis of the duration of fecal shedding and colonization of tissues, of three Arcobacter species in 1-day-old cesarean-derived colostrum-dep...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2295-2299.1996
更新日期:1996-06-01 00:00:00
abstract::Oxygenated fatty acids, or oxylipins, play an essential role in physiological signaling and developmental processes in animals, plants, and fungi. Previous characterization of three Aspergillus fumigatus dioxygenases (PpoA, PpoB, and PpoC), similar in sequence to mammalian cyclooxygenases, showed that PpoA is responsi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00009-08
更新日期:2008-07-01 00:00:00
abstract::Indomethacin, a nonsteroidal anti-inflammatory agent, will abolish salmonella-induced rabbit ileal secretion when given prior to the establishment of infection. In the present study, we assessed whether indomethacin can inhibit salmonella-induced intestinal secretion when administered after infection and net intestina...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.1.136-139.1977
更新日期:1977-07-01 00:00:00
abstract::C57BL/6 mice infected with Leishmania mexicana showed depression of the in vitro immunoglobulin M-plaque-forming cell response to sheep erythrocytes. Immunodepression was present 3 weeks after inoculation and was maximal at 11 weeks. Thereafter, there was a gradual return to normal immunoresponsiveness correlated with...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.415-419.1981
更新日期:1981-05-01 00:00:00
abstract::Recent evidence suggests that immune-mediated gastric epithelial cell apoptosis through Fas-Fas ligand interactions participates in Helicobacter pylori disease pathogenesis. To define the role of Fas signaling in vivo, H. pylori strain SS1 infection in C57BL/6 mice was compared to that in mice deficient in the Fas lig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2591-2597.2002
更新日期:2002-05-01 00:00:00
abstract::The serotype-specific polysaccharide of Streptococcus mutans AHT (serotype a) was shown to be loosely associated with the cell surface of this organism. The antigen was extracted from whole cells by boiling in sodium acetate buffer, pH 4.0, for 10 min. The purified product was found to be a diheteroglycan of galactose...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1177-1185.1979
更新日期:1979-12-01 00:00:00
abstract::Myeloid differentiation protein 88 (MyD88) is a general adaptor for the signaling cascade through receptors of the Toll/IL-1R family. When infected with Leishmania major parasites, MyD88-deficient mice displayed a dramatically enhanced parasite burden in their tissues similar to that found in susceptible BALB/c mice. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.7215-7218.2003
更新日期:2003-12-01 00:00:00
abstract::Neutrophils play a major role in defending the periodontium against infection by oral, gram-negative, facultative bacteria, such as Actinobacillus actinomycetemcomitans, Eikenella corrodens, and Capnocytophaga spp. We examined the sensitivity of these bacteria to a mixture of low-molecular-weight peptides and highly p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.12.3934-3940.1990
更新日期:1990-12-01 00:00:00
abstract::Improvement of antibody responses to polysaccharides through their linkage to proteins is thought to be mediated by protein-specific T helper (Th) cells. To investigate whether the carrier protein of a conjugate could be substituted by a Th epitope, Streptococcus pneumoniae type 17F polysaccharide (PS) was bromoacetyl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.961-968.1995
更新日期:1995-03-01 00:00:00
abstract::A T antigen preparation free of trypsin was obtained by application of CNBr-Sepharose linked to pure trypsin. Purification on an ion exchange chromatography column results in an electrophoretically homogeneous preparation of T protein. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.993-994.1976
更新日期:1976-03-01 00:00:00
abstract::The pattern of invasion of human hair in vitro by the dermatophyte Microsporum gypseum was studied by transmission and scanning electron microscopy. Mycelia that invaded the hair cortex through the edge of cuticles showed a flattened "frond" growth in contrast to the filamentous form seen on ordinary laboratory media....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.706-715.1982
更新日期:1982-11-01 00:00:00
abstract::The incidence of translocation of viable Escherichia coli C25 from the gastrointestinal tract to the mesenteric lymph nodes was compared in gnotobiotic mice colonized with only E. coli C25 and in gnotobiotic mice colonized with E. coli C25 plus the whole cecal flora from specific pathogen-free mice. The population lev...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.3.820-827.1979
更新日期:1979-09-01 00:00:00
abstract::The rate of clearance of Vibrio fetus var. venerealis was measured by using in vivo and in vitro methods. The rate of clearance from the circulating blood increased in immune rabbits and reached a plateau 4 days after immunization. Thereafter in vivo clearance did not increase further despite rising agglutinin titers....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.6.815-818.1971
更新日期:1971-06-01 00:00:00
abstract::Lipoteichoic acids (LTAs) belong to the immunostimulatory class of molecules of gram-positive bacteria (GPB). Previous investigations showed that the macrophage scavenger receptor (SR), a glycosylated trimeric transmembrane protein, binds directly to many GPB, possibly via LTA. SR binding to other ligands is dependent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3318-3325.1996
更新日期:1996-08-01 00:00:00
abstract::Campylobacter jejuni is a leading cause of infectious diarrhea throughout the world. In addition, there is growing evidence that Guillain-Barré syndrome, an inflammatory demyelinating disease of the peripheral nervous system, is frequently preceded by C. jejuni infection. In the present study, the hrcA-grpE-dnaK gene ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1194-1200.1999
更新日期:1999-03-01 00:00:00
abstract::Mutant cultures that lacked the ability to produce filamentous hemagglutinin were obtained by UV irradiation of Bordetella pertussis strain 18-323. Various biological activities of these mutants were compared with those of the parent culture. It was found that the leukocytosis-promoting, histamine-sensitizing, and mou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.1018-1023.1982
更新日期:1982-03-01 00:00:00
abstract::Rocky Mountain spotted fever, a systemic tick-borne illness caused by the obligate intracellular bacterium Rickettsia rickettsii, is associated with widespread infection of the vascular endothelium. R. rickettsii infection induces a biphasic pattern of the nuclear factor-kappaB (NF-kappaB) activation in cultured human...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.155-165.2005
更新日期:2005-01-01 00:00:00
abstract::The type-specific antigens of group B type Ic (old designation type Ii) streptococci were extracted, purified, and characterized by serological and chemical methods. The Ia antigen, shared by types Ia and Ic, is a polysaccharide composed of 69% galactose and 25% glucosamine (i.e., 31% N-acetyl-glucosamine). However, t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.5.596-604.1971
更新日期:1971-11-01 00:00:00
abstract::An experimental model of "chronic" diphtheria intoxication in the guinea pig was developed. Adult guinea pigs, subjected to a regimen of multiple sublethal doses of purified diphtheria toxin (total of 1.4 minimum lethal doses divided equally in four daily doses), developed a toxemia which terminated in death between 6...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.4.556-560.1973
更新日期:1973-04-01 00:00:00
abstract::Utilization of heme-hemopexin as a source of heme by Haemophilus influenzae type b is dependent on expression by this bacterium of the 100-kDa HxuA protein, which is both present on the bacterial cell surface and released into the culture supernatant (L. D. Cope, R. Yogev, U. Muller-Eberhard, and E. J. Hansen, J. Bact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::The bacterial pathogen Burkholderia pseudomallei invades host cells, escapes from endocytic vesicles, multiplies intracellularly, and induces the formation of actin tails and membrane protrusions, leading to direct cell-to-cell spreading. This study was aimed at the identification of B. pseudomallei genes responsible ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01262-05
更新日期:2006-06-01 00:00:00
abstract::Incubation of large granular lymphocytes (LGL) with glutaraldehyde-fixed bacteria stimulated in the supernatant the production of interferon (IFN), which proved to be mainly IFN-gamma. Even though IFN-gamma was produced upon exposure of LGL to bacteria, anti-IFN-gamma antibodies failed to interfere with induction of c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2767-2773.1991
更新日期:1991-08-01 00:00:00