Abstract:
:Oxygenated fatty acids, or oxylipins, play an essential role in physiological signaling and developmental processes in animals, plants, and fungi. Previous characterization of three Aspergillus fumigatus dioxygenases (PpoA, PpoB, and PpoC), similar in sequence to mammalian cyclooxygenases, showed that PpoA is responsible for the production of the oxylipins 8R-hydroperoxyoctadecadienoic acid and 5S,8R-dihydroxy-9Z,12Z-octadecadienoic acid and that PpoC is responsible for 10R-hydroxy-8E,12Z-hydroperoxyoctadecadienoic acid. Here, Delta ppo mutants were characterized to elucidate the role of fungal dioxygenases in A. fumigatus development and host interactions. The Delta ppoC strain displayed distinct phenotypes compared to those of other Delta ppo mutants and the wild type, including altered conidium size, germination, and tolerance to oxidative stress as well as increased uptake and killing by primary alveolar macrophages. These experiments implicate oxylipins in pathogen development and suggest that Delta ppoC represents a useful model for studying the A. fumigatus-host interaction.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Dagenais TR,Chung D,Giles SS,Hull CM,Andes D,Keller NPdoi
10.1128/IAI.00009-08subject
Has Abstractpub_date
2008-07-01 00:00:00pages
3214-20issue
7eissn
0019-9567issn
1098-5522pii
IAI.00009-08journal_volume
76pub_type
杂志文章abstract::Acquisition of immunity to Plasmodium falciparum sexual stages is a key determinant for reducing human-mosquito transmission by preventing the fertilization and the development of the parasite in the mosquito midgut. Naturally acquired immunity against sexual stages may therefore form the basis for the development of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05288-11
更新日期:2011-12-01 00:00:00
abstract::The absolute requirement for elemental iron and the porphyrin nucleus for growth of Haemophilus influenzae led us to investigate the role of iron and hemin in regulation of expression of the H. influenzae transferrin receptor. H. influenzae type b strain H1689 was grown in brain heart infusion broth supplemented with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4033-4037.1993
更新日期:1993-10-01 00:00:00
abstract::The amount of crude Campylobacter jejuni enterotoxin present in culture products was quantitated by comparing the response of these preparations with that of pure Escherichia coli heat-labile toxin (LT) in the Chinese hamster ovary assay and in enzyme-linked immunosorbent assays that used GM ganglioside or antisera to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.2.314-319.1984
更新日期:1984-08-01 00:00:00
abstract::The innate and the adaptive arms of the mucosal immune system must be coordinated to facilitate the control of pathogenic invasion while maintaining immune homeostasis. Toll-like receptors, able to activate the cell to produce bactericidal and inflammatory cytokines but also able to upregulate antigen (Ag)-presenting ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00100-07
更新日期:2008-02-01 00:00:00
abstract::Arthritis is a frequent and major complication of infection with Borrelia burgdorferi sensu stricto. The antigens responsible for the induction of arthritis are unknown. Here we provide direct evidence that a major surface protein, outer surface protein A (OspA), can induce arthritis. Hamsters were vaccinated with 30,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.658-663.2000
更新日期:2000-02-01 00:00:00
abstract::Tissue macrophages are the obligatory host cells for Leishmania donovani, the causative agent of visceral leishmaniasis. In this study we sought to determine whether activated macrophages, as defined by the functional criterion of tumor cell cytotoxicity, were also able to exert a microbicidal effect on ingested L. do...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.3.918-926.1981
更新日期:1981-09-01 00:00:00
abstract::With large amounts of parasite gene sequence available, additional bioinformatic tools to screen these sequences for identifying genes encoding antigens are needed. Proteins containing tandem repeat (TR) domains are often B-cell antigens, and antibody responses toward TR domains of the proteins are dominant in human i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01205-06
更新日期:2007-02-01 00:00:00
abstract::The Leishmania pifanoi amastigote antigen P-8 has been previously shown to induce protective immunity in a murine model of cutaneous leishmaniasis (L. Soong, S. M. Duboise, P. Kima, and D. McMahon-Pratt, Infect. Immun. 63:3559-3566, 1995). As this antigen is of interest for further vaccine studies, the biochemical cha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.11.6776-6784.2001
更新日期:2001-11-01 00:00:00
abstract::Although the suckling mouse assay is widely used for the detection of heat-stable Escherichia coli enterotoxin (ST), few data have been published concerning the reproducibility, optimal growth, and test conditions of this assay. Four strains of toxigenic E. coli known to elaborate both heat-labile enterotoxin and ST o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.1.95-99.1976
更新日期:1976-07-01 00:00:00
abstract::Susceptibility to Coccidioides spp. varies widely in humans and other mammals and also among individuals within a species. Among strains of mice with various susceptibilities, immunohistopathology revealed that C57BL/6 mice were highly susceptible to the disease following intranasal infection, DBA/2n mice were interme...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00885-08
更新日期:2008-12-01 00:00:00
abstract::Photobacterium damselae subsp. damselae causes infections and fatal disease in marine animals and in humans. Highly hemolytic strains produce damselysin (Dly) and plasmid-encoded HlyA (HlyA(pl)). These hemolysins are encoded by plasmid pPHDD1 and contribute to hemolysis and virulence for fish and mice. In this study, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00155-13
更新日期:2013-09-01 00:00:00
abstract::The development and course of normally lethal parasitemias in mice inoculated intraperitoneally with erythrocytic stages of Plasmodium yoelii or Plasmodium berghei were markedly affected by treatment with the antilymphoid drug cyclosporin A (CS-A). When the first of four daily subcutaneous 25-mg/kg doses of CS-A was g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.1093-1100.1982
更新日期:1982-09-01 00:00:00
abstract::The purpose of this study was to determine the effects of human monocytes, macrophages, and polymorphonuclear neutrophils (PMN) on the fungal pathogen Blastomyces dermatitidis in vitro. Peripheral blood monocyte monolayers significantly inhibited the replication of a virulent strain (V) and an avirulent strain (AV) of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.1.297-303.1982
更新日期:1982-04-01 00:00:00
abstract::Neisseria gonorrhoeae is generally considered to be an obligate aerobe; it can, however, grow in the absence of oxygen by anaerobic respiration by using nitrite as a terminal electron acceptor. The outer membrane protein compositions of aerobically and anaerobically grown N. gonorrhoeae strains were compared by one- a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1359-1364.1987
更新日期:1987-06-01 00:00:00
abstract::Most Helicobacter pylori strains express the BabA adhesin, which binds to ABO/Leb blood group antigens on gastric mucin and epithelial cells and is found more commonly in strains that cause peptic ulcers or gastric cancer, rather than asymptomatic infection. We and others have previously reported that in mice, gerbils...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00094-17
更新日期:2017-05-23 00:00:00
abstract::Ultrastructural immunoperoxidase studies were done in spinal cords of mice infected with wild type vesicular stomatitis virus or its temperature-sensitive (ts) mutant G31. Infected neurons showed subplasmalemmal staining of viral antigen and staining of viral particles budding from the neuronal membrane in wild-type v...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.276-281.1979
更新日期:1979-04-01 00:00:00
abstract::Commercial fluid used for peritoneal lavage in peritonitis and in peritoneal dialysis suppressed the activity of peripheral blood leukocytes as measured by chemiluminescence, phagocytosis, and bacterial killing. Suppression was found to be due to the low pH and high osmolality of the fluid. The pH was adjusted to noni...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.130-135.1981
更新日期:1981-07-01 00:00:00
abstract::The adherence of several strains of Escherichia coli to human buccal epithelial cells was studied, using cells obtained from five groups: healthy adults, healthy children, children with acute diarrhea, children with persistent diarrhea associated with cryptosporidial parasites, and children with noncryptosporidial per...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4793-4800.1992
更新日期:1992-11-01 00:00:00
abstract::Isolated outer membranes of Borrelia burgdorferi 297 were utilized to obtain partial amino acid sequence information for a low-molecular-weight, outer membrane-associated polypeptide. Degenerate oligonucleotide primers based upon this information were used to amplify a 100-bp probe for detection of the corresponding f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.412-421.1997
更新日期:1997-02-01 00:00:00
abstract::Brucella abortus and polyacrylic acid, two agents whose interferon-inducing capacity in the mouse has been well established, failed to stimulate circulating interferon production in rabbits, yet protected rabbits against local vaccinia virus infection. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.4.669-673.1973
更新日期:1973-10-01 00:00:00
abstract::The glycolipid haptens of Mycoplasma pneumoniae became immunogenic when bound to membrane proteins of Acholeplasma laidlawii by reaggregation. This process consisted of the solubilization of lipid-depleted A. laidlawii membranes and M. pneumoniae glycolipids in 20 mm sodium dodecyl sulfate and dialysis of the mixed so...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.3.420-423.1971
更新日期:1971-03-01 00:00:00
abstract::Brucella abortus injected into CBA mice replicated primarily in the spleen and liver, reaching a peak bacterial count in both organs about 7 days postinfection. The organism was eliminated from the liver but declined to a chronic phase in the spleen. The infection caused hepatosplenomegaly. An influx of macrophages in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1465-1472.1992
更新日期:1992-04-01 00:00:00
abstract::Previous studies have demonstrated antigenic differences among the pili expressed by various strains of Haemophilus influenzae type b (Hib). In order to understand the molecular basis for these differences, the structural gene for pilin was cloned from Hib strain Eagan (p+) and the nucleotide sequence was compared to ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.1991-1996.1991
更新日期:1991-06-01 00:00:00
abstract::Insertional inactivation of the Streptococcus mutans spaP gene was used to construct an isogenic mutant (834) of strain NG8 (serotype c) which lacked the major cell surface-associated protein referred to as P1 (15). Results of several studies suggest that P1 is involved in the adherence of S. mutans to saliva-coated a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.12.4606-4609.1991
更新日期:1991-12-01 00:00:00
abstract::We previously demonstrated that plasmid-deficient Chlamydia muridarum retains the ability to infect the murine genital tract but does not elicit oviduct pathology because it fails to activate Toll-like receptor 2 (TLR2). We derived a plasmid-cured derivative of the human genital isolate Chlamydia trachomatis D/UW-3/Cx...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01118-10
更新日期:2011-03-01 00:00:00
abstract::Gamma interferon (IFN-gamma), a pleiotropic cytokine, is now known to be produced by macrophages as well as by NK cells, gammadelta cells, and activated T cells. The autocrine biological functions of IFN-gamma on the macrophage include the upregulation of major histocompatibility complex MHC class II and the activatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3605-3610.2001
更新日期:2001-06-01 00:00:00
abstract::Brucellosis is a costly disease of water buffaloes (Bubalus bubalis). Latent infections and prolonged incubation of the pathogen limit the efficacy of programs based on the eradication of infected animals. We exploited genetic selection for disease resistance as an approach to the control of water buffalo brucellosis....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.4.2115-2120.2006
更新日期:2006-04-01 00:00:00
abstract::The search to identify Mycobacterium tuberculosis antigens capable of conferring protective immunity against tuberculosis has received a boost owing to the resurgence of tuberculosis over the past two decades. It has long been recognized that lymphoid cells are required for protection against M. tuberculosis. While tr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.1929-1937.2003
更新日期:2003-04-01 00:00:00
abstract::Pneumonic plague, caused by inhalation of Yersinia pestis, represents a major bioterrorism threat for which no vaccine is available. Based on the knowledge that genetic delivery of monoclonal antibodies (MAbs) with adenovirus (Ad) gene transfer vectors results in rapid, high-level antibody expression, we evaluated the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00856-08
更新日期:2009-04-01 00:00:00
abstract::Influenza A/Scotland/74 (H3N2) and A/Victoria/75 (H3N2) cold-adapted (ca) recombinant viruses, prepared by mating the A/Ann Arbor/6/60 (H2N2) ca donor virus and influenza A wild-type virus, were evaluated in adult seronegative volunteers (serum hemagglutination-inhibiting antibody titer, =1:8) for level of attenuati...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.2.253-259.1979
更新日期:1979-02-01 00:00:00