Abstract:
:The amount of crude Campylobacter jejuni enterotoxin present in culture products was quantitated by comparing the response of these preparations with that of pure Escherichia coli heat-labile toxin (LT) in the Chinese hamster ovary assay and in enzyme-linked immunosorbent assays that used GM ganglioside or antisera to LT or both. Maximum C. jejuni enterotoxin production was achieved by growth at 42 degrees C for 24 h under agitation in supplemented GC medium. Adding polymyxin separately to either the broth supernatant or the cells enhanced the recovery of toxin; the yield from cell lysates was much lower. The quantity of C. jejuni enterotoxin produced by clinical isolates obtained locally or provided from Mexico varied widely, over a spectrum from none to large amounts; quantitative values for the amount of C. jejuni enterotoxin determined by the Chinese hamster ovary and enzyme-linked immunosorbent assays correlated with the degree of secretory potency of this material in ligated rat ileal loops. The cytotonic activity of C. jejuni enterotoxin in Chinese hamster ovary cells was abolished by heating at 96 degrees C for 10 min and by preincubation either with GM ganglioside or with LT or cholera toxin antisera. The secretory activity of C. jejuni enterotoxin in ligated rat ileal loops was passively neutralized by antiserum to LT, and immunizing rats with either LT or its B subunit significantly (P less than 0.001) reduced fluid response to active challenge with C. jejuni enterotoxin in ligated ileal loops. These observations indicate that strains of C. jejuni vary in their capacity to elaborate a heat-labile enterotoxin that has close immunological homology with LT and cholera toxin.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Klipstein FA,Engert RFdoi
10.1128/IAI.45.2.314-319.1984subject
Has Abstractpub_date
1984-08-01 00:00:00pages
314-9issue
2eissn
0019-9567issn
1098-5522journal_volume
45pub_type
杂志文章abstract::The tetrasaccharide 3-deoxy-alpha-D-manno-2-octulosonic acid (alpha-KDO) (2----8)-alpha-KDO(2----4)-alpha-KDO(2----6)-beta GlcNAc, a partial structure of chlamydial lipopolysaccharide (LPS) representing a genus-specific epitope, was synthesized and covalently linked to bovine serum albumin, resulting in an artificial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1314-1321.1992
更新日期:1992-04-01 00:00:00
abstract::Production of the Kanagawa hemolysin by patient strains of Vibrio parahaemolyticus was found to respond to the pH rather than to the type of carbohydrate present in the growth medium. Regardless of the carbohydrate present, hemolysin production in peptone broth cultures occurred only when the pH was between 6.5 and 5....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.115-119.1981
更新日期:1981-10-01 00:00:00
abstract::A cytotoxigenic Clostridium difficile strain that fails to produce toxin A but causes hemorrhage and bloody fluid accumulation in ligated ileal loops of rabbits and hemorrhage and diarrhea in hamsters is described. The lack of reaction of DNA from this strain in hybridization studies with a toxin A gene-specific 4.5-k...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.10.4192-4199.1992
更新日期:1992-10-01 00:00:00
abstract::A better knowledge on how immune responses are initiated in mucosal tissues would facilitate the design of new mucosal vaccines, as well as improve our understanding on host defense against infection. We investigated the mechanisms of adjuvanticity of the Mycoplasma-derived macrophage-activating 2-kDa lipopeptide (MAL...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.6978-6986.2004
更新日期:2004-12-01 00:00:00
abstract::Cryptosporidium parvum is a coccidian parasite that causes diarrheal disease in many vertebrate species, including young (less than or equal to 1 month old) calves. Older calves and adult cattle are resistant to infection. In this study, newborn calves were raised in isolation from C. parvum for 1 week to 3 months bef...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2237-2240.1990
更新日期:1990-07-01 00:00:00
abstract::The antigenic cross-reactivity between Treponema pallidum and several pathogenic members of the family Spirochaetaceae was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques. Blots of T. pallidum antigens were incubated with antiserum from rabbits infected or immunize...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.1.116-121.1984
更新日期:1984-10-01 00:00:00
abstract::Studies of meningococcal pathogenesis have been severely restricted due to the absence of an adequate animal model. Given the significance of iron in meningococcal pathogenesis, we developed a model of Neisseria meningitidis colonization in outbred adult mice that included daily administration of iron dextran. While r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.1849-1855.2003
更新日期:2003-04-01 00:00:00
abstract::Bacillus anthracis, the causal agent of anthrax, synthesizes two surface layer (S-layer) proteins, EA1 and Sap, which account for 5 to 10% of total protein and are expressed in vivo. A recombinant B. anthracis strain was constructed by integrating into the chromosome a translational fusion harboring the DNA fragments ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4847-4850.1999
更新日期:1999-09-01 00:00:00
abstract::Conditions for the release of streptococcal type 12 M protein from whole cells by cyanogen bromide are described; they demonstrated that methionine is not essential to the structural arrangements which account for some of its immunological and biological properties. The released M protein was separated from other prot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.546-555.1978
更新日期:1978-08-01 00:00:00
abstract::The salivary and serum immune responses to orally administered glucosyltransferase antigen complex from Streptococcus mutants strain 6715 were investigated in hamsters. All enzyme-linked immunosorbent assay was used to measure the antibody quantity and isotype, and a [14C]glucosyl-labeled sucrose incorporation assay w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Brucella spp. are gram-negative intracellular pathogens that survive and multiply within phagocytic cells of their hosts. Smooth organisms present O polysaccharides (OPS) on their surface. These OPS help the bacteria avoid the bactericidal action of serum. The wboA gene, coding for the enzyme glycosyltransferase, is e...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4407-4416.2001
更新日期:2001-07-01 00:00:00
abstract::High levels of Treponema denticola in subgingival dental plaque are associated with severe periodontal disease. T. denticola, along with Porphyromonas gingivalis and Bacteroides forsythus, are the only cultivatable oral microorganisms that produce significant amounts of "trypsin-like" peptidase activity. The ability o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.10.6193-6200.2001
更新日期:2001-10-01 00:00:00
abstract::Membrane glycolipids contain the lactose sequence (galactose linked to glucose), and the oligosaccharide is variously extended such that there is a cell-type-specific repertoire. In this study, binding of Pseudomonas aeruginosa M35 to lipid-linked lactose (Gal beta 1-4Glc [structure 1]), lacto-N-neotetraose (Gal beta ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5078-5084.1992
更新日期:1992-12-01 00:00:00
abstract::Although Streptococcus intermedius and Streptococcus mutans are regarded as members of the commensal microflora of the body, S. intermedius is often associated with deep-seated purulent infections, whereas S. mutans is frequently associated with dental caries. In this study, we investigated the roles of the S. mutans ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.1.249-256.2002
更新日期:2002-01-01 00:00:00
abstract::Salmonella infection can cause gastroenteritis in healthy individuals or a serious, systemic infection in immunocompromised patients and has a global impact. CD4 Th1 cells represent the main lymphocyte population that participates in bacterial clearance during both primary and secondary infections in mice of the H-2b ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00588-19
更新日期:2019-10-18 00:00:00
abstract::We characterized outer membrane proteins (OMPs) from selected Campylobacter jejuni, C. coli, and C. fetus strains by two-dimensional gel electrophoresis (2DGE), using isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and by immunoblotting with immune rabbit serum. The flage...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract::The absolute requirement for elemental iron and the porphyrin nucleus for growth of Haemophilus influenzae led us to investigate the role of iron and hemin in regulation of expression of the H. influenzae transferrin receptor. H. influenzae type b strain H1689 was grown in brain heart infusion broth supplemented with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4033-4037.1993
更新日期:1993-10-01 00:00:00
abstract::Staphylococcus aureus biofilm formation is induced in iron-restricted growth conditions in vitro. In this study, we showed that Emp and Eap play important roles in low-iron-induced biofilm formation of S. aureus Newman. Eap and Emp are secreted proteins which are non-covalently attached to the S. aureus cell surface a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01635-07
更新日期:2008-04-01 00:00:00
abstract::Cells of several strains of Streptococcus gordonii attached in much higher numbers to experimental pellicles formed from samples of submandibular or parotid saliva on hydroxyapatite (HA) beads than to buffer controls. The nature of the salivary components responsible were investigated by preparing experimental pellicl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.2948-2954.1991
更新日期:1991-09-01 00:00:00
abstract::Ribosomal subunits from Mycobacterium smegmatis were analyzed by using sedimentation velocity, sedimentation equilibrium, and acrylamide gel electrophoresis experiments. These s(20.w) values for the subunits are 48.7S and 28.1S. The molecular weight of the 49S subunit is about 1.65 x 10(6), and that of the 28S subunit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.3.489-496.1974
更新日期:1974-03-01 00:00:00
abstract::When spleen cells derived from C57BL/6 mice immunized with L cells 7 days previously were cocultured with antigenic cells, immune interferon appeared in the culture fluid. We analyzed the tissue distribution of the immune interferon-producing cells (IIPC) which appeared in various lymphoid organs after allogeneic stim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Group A streptococci isolated from skin adhere in greater numbers to human skin epithelial cells than to cells obtained from buccal mucosa whereas streptococci isolated from a throat tend to adhere in greater numbers to buccal epithelial cells than to skin epithelial cells in vitro. M protein-producing strains of grou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.2.555-557.1977
更新日期:1977-11-01 00:00:00
abstract::Purified Escherichia coli type 1 fimbriae have been shown previously to stimulate T-cell-independent proliferation of human B lymphocytes. The response is mediated by the mannose-specific, lectin-like adhesin protein FimH. Here we show that type 1 fimbriae also stimulate immunoglobulin (Ig) secretion by B cells. The r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5197-5203.1992
更新日期:1992-12-01 00:00:00
abstract::Numerous bacterial pathogens use type III secretion systems (T3SSs) or T4SSs to inject or translocate virulence proteins into eukaryotic cells. Several different reporter systems have been developed to measure the translocation of these proteins. In this study, a peptide tag-based reporter system was developed and use...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00690-06
更新日期:2006-10-01 00:00:00
abstract::Lyme disease (LD), the most prevalent tick-borne illness in North America, is caused by Borrelia burgdorferi The long-term survival of B. burgdorferi spirochetes in the mammalian host is achieved though VlsE-mediated antigenic variation. It is mathematically predicted that a highly variable surface antigen prolongs ba...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00890-16
更新日期:2016-12-29 00:00:00
abstract::Iron acquisition from the host is an important step in the pathogenic process. While Yersinia pestis has multiple iron transporters, the yersiniabactin (Ybt) siderophore-dependent system plays a major role in iron acquisition in vitro and in vivo. In this study, we determined that the Ybt system is required for the us...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01236-09
更新日期:2010-05-01 00:00:00
abstract::Adoptive immunity is poorly expressed in normal syngeneic mice. This phenomenon was studied by using experimental antituberculosis immunity as a model system representing pure cell-mediated immunity. Expression of adoptive immunity was facilitated by pretreating recipients with sublethal ionizing radiation (500 rads) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.1.257-263.1983
更新日期:1983-07-01 00:00:00
abstract::gammaG-globulin from nonimmunized rabbits and from rabbits immunized with various bacteria reacted in the immunofluorescence technique with protein A-containing Staphylococcus aureus. Pepsin digestion of most immunoglobulin preparations eliminated the reaction, thus showing that the Fc fragment is involved and that th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.4.387-391.1970
更新日期:1970-10-01 00:00:00
abstract::Sublethal infection of mice with recombinant Listeria monocytogenes expressing a model epitope in either secreted or nonsecreted form results in similar CD8(+) T-cell priming. Since nonsecreted bacterial proteins have no obvious access to the endogenous major histocompatibility complex (MHC) class I presentation pathw...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2196-2204.2000
更新日期:2000-04-01 00:00:00
abstract::Metastatic infection after hematogenous dissemination of Candida species is presumably dependent on the fungus traversing the vascular endothelium. An in vitro model of the earliest events of metastatic Candida infection was developed with whole vascular strips. Freshly obtained porcine blood vessels were secured in a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.374-384.1983
更新日期:1983-10-01 00:00:00