Abstract:
:Conditions for the release of streptococcal type 12 M protein from whole cells by cyanogen bromide are described; they demonstrated that methionine is not essential to the structural arrangements which account for some of its immunological and biological properties. The released M protein was separated from other proteins by column chromatography with hydroxylapatite. The type-specific molecules which reacted with precipitating antibodies were found only in the 0.3 M eluate, formed zones with mobilities less than 12% of that of the dye front on electrophoresis in the standard acrylamide disc gel system, formed at least four bands in sodium dodecyl sulfate-acrylamide disc gels with molecular weights ranging from 12,000 to 23,000, and stimulated the formation of opsonic antibodies in rabbits. Cyanogen bromide provides a highly specific method for the release of M proteins which should prove particularly useful in analyses of structural-functional relationships among different M proteins.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Vosti KL,Williams WKdoi
10.1128/IAI.21.2.546-555.1978subject
Has Abstractpub_date
1978-08-01 00:00:00pages
546-55issue
2eissn
0019-9567issn
1098-5522journal_volume
21pub_type
杂志文章abstract::Previous studies have shown that tumor necrosis factor alpha (TNF-alpha) plays a pathophysiologic role in sepsis induced in rat pups by group B streptococci (GBS). In this model, TNF-alpha is also partially responsible for the induction of interleukin-6 (IL-6). The present study was undertaken to investigate the role ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4997-5002.1994
更新日期:1994-11-01 00:00:00
abstract::Haemophilus influenzae type b infection of immersed and air interface organ cultures for 24 h caused significant epithelial damage. Bacterial association with mucus, damaged epithelium, and unciliated cells was significantly higher in air interface than immersed organ cultures, and total bacterial association was 55.8...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2353-2355.1996
更新日期:1996-06-01 00:00:00
abstract::Protection against cholera has been correlated with the level of serum vibriocidal antibodies. The specificity of these vibriocidal antibodies was mostly to the lipopolysaccharide (LPS). We synthesized conjugates of detoxified LPS with cholera toxin (CT) and other proteins in order to elicit serum LPS antibodies with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3201-3208.1992
更新日期:1992-08-01 00:00:00
abstract::The protozoan parasite Entamoeba histolytica causes amebic dysentery and amebic liver abscess, diseases associated with significant morbidity and mortality worldwide. E. histolytica infection appears to involve the initial attachment of amebic trophozoites to intestinal epithelial cells, followed by lysis of these cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.5.1631-1639.1997
更新日期:1997-05-01 00:00:00
abstract::Yersinia pestis causes a rapid, lethal disease referred to as plague. Y. pestis actively inhibits the innate immune system to generate a noninflammatory environment during early stages of infection to promote colonization. The ability of Y. pestis to create this early noninflammatory environment is in part due to the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00909-19
更新日期:2020-02-20 00:00:00
abstract::Eikenella corrodens normally inhabits the human respiratory and gastrointestinal tracts but is frequently the cause of abscesses at various sites. Using the N-terminal portion of the Moraxella nonliquefaciens pilin gene as a hybridization probe, we cloned two tandemly located pilin genes of E. corrodens 31745, ecpC an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.1909-1916.1993
更新日期:1993-05-01 00:00:00
abstract::Livers of normal mice trapped over 80% of intravenously injected 51Cr-labeled lipopolysaccharide after 1 h. Liver fractionation studies showed that nearly 45% of the labeled endotoxin was associated with cell nuclei, 20% with the mitochondrial-lysosomal fraction, and approximately 30% with the cell sap. Analysis of th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.1.100-105.1976
更新日期:1976-07-01 00:00:00
abstract::The appearance of lymphotoxin in the culture fluid of phytohemagglutinin (PHA)-stimulated mouse spleen cells required two cell fractions that were separated by adherence to plastic. Upon stimulation with PHA, neither cell fraction alone produced significant amounts of lymphotoxin; however, when the cell fractions were...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.3.1028-1035.1982
更新日期:1982-06-01 00:00:00
abstract::Cholera toxin (CT) and related Escherichia coli enterotoxins LTI and LTIIb have a conserved hydrophobic region at the AB(5) interface postulated to be important for toxin assembly. Hydrophobic residue F223 in the A subunit of CT (CTA) as well as residues 174, L77, and T78 in the B subunit of CT (CTB) were replaced ind...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.4093-4101.2003
更新日期:2003-07-01 00:00:00
abstract::The yeast form of the dimorphic fungus Histoplasma capsulatum survives within macrophages after phagocytosis. To do so, it must avoid, inhibit, or resist a variety of toxic oxygen metabolites. Using ferricytochrome c reduction to assay superoxide release, we examined the response of mouse macrophages to the yeast form...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.1.29-34.1987
更新日期:1987-01-01 00:00:00
abstract::The cytoskeletal lesions associated with enteropathogenic Escherichia coli adhering to cultured HeLa epithelial cells were examined by immunofluorescence microscopy. The microfilament-associated proteins actin, alpha-actinin, talin, and ezrin were localized with adherent enteropathogenic E. coli, whereas tropomyosin, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.6.2541-2543.1992
更新日期:1992-06-01 00:00:00
abstract::We report the immunizing capacity of Salmonella enteritidis AL1192, a strain that has been cured of a 36-megadalton plasmid, to protect ddY mice against subsequent challenge with virulent salmonellas. This strain, which was given subcutaneously at a dose of 10(6) organisms, provided significant protection against oral...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.586-587.1985
更新日期:1985-11-01 00:00:00
abstract::Interleukin-18 (IL-18) is a proinflammatory cytokine that plays an important role in natural killer cell activation and the T helper 1 (Th1) cell response, particularly in collaboration with IL-12. Since Th1 cells play a pivotal role in the host defense against infection with intracellular microbes, such as Leishmania...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2449-2456.2000
更新日期:2000-05-01 00:00:00
abstract::Campylobacter fetus surface layer proteins (SLPs), encoded by sapA homologues, are important in virulence. In wild-type C. fetus strain 23D, all eight sapA homologues are located in the 54-kb sap island, and SLP expression reflects the position of a unique sapA promoter in relation to the sapA homologues. The extensiv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.3.1715-1724.2004
更新日期:2004-03-01 00:00:00
abstract::Immunization with ribosomal preparations from Haemophilus influenzae type b elicited protective immunity in mice. Ribosomes from disrupted cells where isolated by differential centrifugation using sodium dodecyl sulfate. The washed ribosomes contained 25% protein and 75% ribonucleic acid and sedimented as a single pea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.2.453-460.1977
更新日期:1977-02-01 00:00:00
abstract::Three types of caseinolytic proteases (Pase-A, Pase-B, and Pase-C) were isolated and purified from culture supernatants of Porphyromonas gingivalis 381 by the combined procedures of acetone precipitation, gel filtration, solubilization with octylthioglucoside followed by affinity chromatography on arginine-Sepharose 4...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.3060-3068.1991
更新日期:1991-09-01 00:00:00
abstract::Sixty-one patients with a variety of different illnesses were studied with respect to skin test anergy and the presence of serum chemotactic inhibitors. In initial testing, 55% of the patient tests demonstrated negative skin test responses to all six test antigens. Sera from 65% of these anergic patients were capable ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.5.1003-1009.1974
更新日期:1974-11-01 00:00:00
abstract::MtsABC is a Streptococcus pyogenes ABC transporter which was previously shown to be involved in iron and zinc accumulation. In this study, we showed that an mtsABC mutant has impaired growth, particularly in a metal-depleted medium and an aerobic environment. In metal-depleted medium, growth was restored by the additi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2656-2664.2003
更新日期:2003-05-01 00:00:00
abstract::Strains of Cryptococcus neoformans vary in resistance to phagocytosis in vitro. The binding of isolated capsular polysaccharide (CPS) to a capsule-free mutant of C. neoformans confers resistance to phagocytosis. The importance of capsule composition to differences among strains in susceptibility to phagocytosis was ev...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.12.3751-3756.1989
更新日期:1989-12-01 00:00:00
abstract::A set of strain- and size-variant highly immunogenic membrane surface protein antigens of Mycoplasma bovis, which has been identified by a monoclonal antibody, is shown in this report to make up a family of antigenically and structurally related lipid-modified proteins, designated Vsps (variable surface proteins). By ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.5075-5084.1994
更新日期:1994-11-01 00:00:00
abstract::Antibodies against the Plasmodium falciparum P0 ribosomal phosphoprotein (PfP0) have been detected exclusively but extensively in malaria-immune persons. Polyclonal rabbit and mice sera were raised against two recombinant polypeptides of P. falciparum P0 protein, PfP0N and PfP0C, covering amino acids 17 to 61 and the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4312-4318.2000
更新日期:2000-07-01 00:00:00
abstract::Members of the babesial rhoptry-associated protein 1 (RAP-1) family express surface-exposed B-cell epitopes and are candidate antigens for vaccine development. The relationship between rap-1 genomic polymorphism and surface-exposed B-cell epitope expression was analyzed by comparison of biological clones of Mexico str...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3576-3579.1994
更新日期:1994-08-01 00:00:00
abstract::Lipid compositions of 40 patient isolates of Mycobacterium tuberculosis derived from Madras, Burma, Rangoon, and East Africa were studied, and two major populations of tubercle bacilli were distinguished. Nearly all of the strains previously designated as attenuated for the guinea pig (D. A. Mitchison) are characteriz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.1.150-158.1974
更新日期:1974-01-01 00:00:00
abstract::The virulence plasmids of Salmonella typhimurium and other invasive Salmonella serovars have long been associated with the ability of these bacteria to cause systemic infection beyond the intestines in orally inoculated animals. Genetic analysis of virulence genes on the high-molecular-weight plasmids has revealed tha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.2.504-511.1993
更新日期:1993-02-01 00:00:00
abstract::Lesions caused by Leishmania amazonensis normally heal, but relapses occur due to parasite persistence in host tissues. It has been proposed that infection of fibroblasts plays an important role in this process by providing the parasites with a safe haven in which to replicate. However, most previous studies have focu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4571-4580.2002
更新日期:2002-08-01 00:00:00
abstract::In vitro or animal models have been used to investigate the pathogenesis of Helicobacter pylori infection. However, extrapolation to humans of results obtained with these heterologous models remains difficult. We have developed a new model for the study of H. pylori infection that uses human entire embryonic stomachs ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Candida albicans secreted aspartyl proteinases (Saps) are considered virulence-associated factors. Several members of the Sap family were claimed to play a significant role in the progression of candidiasis established by the hematogenous route. This assumption was based on the observed attenuated virulence of sap-nul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00248-10
更新日期:2010-11-01 00:00:00
abstract::Streptococcus pyogenes is one of the most frequent human pathogens. Recent studies have identified dendritic cells (DCs) as important contributors to host defense against S. pyogenes. The objective of this study was to identify the receptors involved in immune recognition of S. pyogenes by DCs. To determine whether To...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01680-07
更新日期:2008-06-01 00:00:00
abstract::Lipopolysaccharide (LPS) is an amphipathic macromolecule that is highly aggregated in aqueous preparations. LPS-binding protein (LBP) catalyzes the transfer of single LPS molecules, segregated from an LPS aggregate, to high-density lipoproteins (HDL), which results in the neutralization of LPS. When fluorescein isothi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.4954-4960.2000
更新日期:2000-09-01 00:00:00
abstract::Purified and concentrated Venezuelan equine encephalitis (VEE) virus derived from tissue cultures, rendered noninfectious by ionizing radiation with retention of in vitro serological activity, also retained a high level of immunogenicity. In mice, fluid vaccines afforded excellent protection against lethal challenge w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.4.574-579.1971
更新日期:1971-04-01 00:00:00