Abstract:
:Lipopolysaccharide (LPS) is an amphipathic macromolecule that is highly aggregated in aqueous preparations. LPS-binding protein (LBP) catalyzes the transfer of single LPS molecules, segregated from an LPS aggregate, to high-density lipoproteins (HDL), which results in the neutralization of LPS. When fluorescein isothiocyanate-labeled LPS (FITC-LPS) is used, this transfer of LPS monomers to HDL can be measured as an increase in fluorescence due to dequenching of FITC-LPS. Recently, serum amyloid P component (SAP) was shown to neutralize LPS in vitro, although only in the presence of low concentrations of LBP. In this study, we show that SAP prevented HDL-mediated dequenching of FITC-LPS, even in the presence of high concentrations of LBP. Human bactericidal/permeability-increasing protein (BPI), a very potent LPS-binding and -neutralizing protein, also prevented HDL-mediated dequenching of FITC-LPS. Furthermore, SAP inhibited HDL-mediated neutralization of both rough and smooth LPS in a chemiluminescence assay quantifying the LPS-induced priming of neutrophils in human blood. SAP bound both isolated HDL and HDL in serum. Using HDL-coated magnetic beads prebound with SAP, we demonstrated that HDL-bound SAP prevented the binding of LPS to HDL. We suggest that SAP, by preventing LPS binding to HDL, plays a regulatory role, balancing the amount of LPS that, via HDL, is directed to the adrenal glands.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
de Haas CJ,Poppelier MJ,van Kessel KP,van Strijp JAdoi
10.1128/iai.68.9.4954-4960.2000keywords:
subject
Has Abstractpub_date
2000-09-01 00:00:00pages
4954-60issue
9eissn
0019-9567issn
1098-5522journal_volume
68pub_type
杂志文章abstract::The attachment of radioisotope-labeled Mycoplasma pneumoniae to hamster tracheal rings in organ culture was examined by radioautography and liquid scintillation counting. Radioautographs of individual rings exposed for 8 h to (3H) thymidine-labeled virulent M. pneumoniae revealed a dense extracellular collection of em...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.959-966.1976
更新日期:1976-03-01 00:00:00
abstract::Sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns of proteins and externally exposed labeled surface constituents were analyzed in promastigotes of three etiological agents of kala azar (Leishmania donovani, HS70 strain from India; L. chagasi, Imperatriz strain from Brazil; L. infantum, ITMPA K263 str...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.136-141.1985
更新日期:1985-10-01 00:00:00
abstract::Legionnaires' disease is clinically manifested as severe pneumonia caused by Legionella pneumophila. However, the dendritic cell (DC)-centered immunological framework of the host defense against L. pneumophila has not been fully delineated. For this study, we focused on a potent chemoattractant for lymphocytes, fracta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5350-5357.2005
更新日期:2005-09-01 00:00:00
abstract::We utilized magnetic resonance imaging to visualize lesions in the lungs of guinea pigs infected by low-dose aerosol exposure to Mycobacterium tuberculosis. Lesions were prominent in such images, and colorized three-dimensional reconstructions of images revealed a very uniform distribution in the lungs. Lesion numbers...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.10.5963-5971.2004
更新日期:2004-10-01 00:00:00
abstract::Chlamydial inclusions were demonstrated by indirect immunofluorescence (IF) with antiserum to the chlamydial group antigen when McCoy cell monolayers infected with either Chlamydia trachomatis or Chlamydia psittaci were fixed in formaldehyde or paraformaldehyde, provided the monolayer was not allowed to dry. If these ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.561-570.1981
更新日期:1981-11-01 00:00:00
abstract::Toxic shock syndrome-associated staphylococcal and streptococcal exotoxins were tested for an ability to induce the production of tumor necrosis factor (TNF). Staphylococcal enterotoxins B and C1, along with streptococcal pyrogenic exotoxin A, all induced TNF production in a dose-dependent manner, with production peak...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.1.291-294.1989
更新日期:1989-01-01 00:00:00
abstract::Ocular inflammation is one of the consequences of infection with the protozoan parasite Toxoplasma gondii. Even if lesions are self-healing in immunocompetent persons, they pose a lifetime risk of reactivation and are a serious threat to vision. As there are virtually no immunological data on reactivating ocular toxop...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02985-14
更新日期:2015-05-01 00:00:00
abstract::Serotype b-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans Y4 consists of D-fucose and L-rhamnose. To clarify the role of SPA in phagocytosis of the organism by human polymorphonuclear leukocytes (PMNs), monoclonal antibodies (MAbs) against SPA and SPA-defective mutants, which were constr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4589-4594.1995
更新日期:1995-12-01 00:00:00
abstract::Mycoplasma genitalium is a sexually transmitted pathogen associated with several acute and chronic reproductive tract disease syndromes in men and women. To evaluate the suitability of a pig-tailed macaque model of M. genitalium infection, we inoculated a pilot animal with M. genitalium strain G37 in the uterine cervi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01322-12
更新日期:2013-08-01 00:00:00
abstract::A major obstacle to studying the functions of particular gene products in the mouse-tick infectious cycle of Borrelia burgdorferi has been an inability to knock out genes in pathogenic strains. Here, we investigated conditions for site-directed mutagenesis in B31 MI, the low-passage-number, infectious B. burgdorferi s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.2139-2150.2002
更新日期:2002-04-01 00:00:00
abstract::Anti-NMDA receptor (NMDAR) autoantibodies have been postulated to play a role in the pathogenesis of NMDAR hypofunction, which contributes to the etiology of psychotic symptoms. Toxoplasma gondii is a pathogen implicated in psychiatric disorders and associated with elevation of NMDAR autoantibodies. However, it remain...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00398-18
更新日期:2018-09-21 00:00:00
abstract::We tested the hypothesis that the intensity of specific antichlamydial T cell-mediated immunity in the genital tract of female guinea pigs infected intravaginally with the chlamydial agent of guinea pig inclusion conjunctivitis would determine the resistance or susceptibility to reinfection after a primary chlamydial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1346-1351.1991
更新日期:1991-04-01 00:00:00
abstract::Patent filarial infection is associated with the downregulation of parasite-specific immune reactivity. In the present study, the relationship between in vitro parasite antigen-specific humoral and cellular immune responsiveness was investigated in Brugia pahangi-infected jirds and in jirds immunized with soluble anti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3052-3057.1988
更新日期:1988-12-01 00:00:00
abstract::The development of vaccines for prevention of diseases caused by pathogenic species can encounter major obstacles if high sequence diversity is observed between individual strains. Therefore, development might be restricted either to conserved antigens, which are often rare, or to multivalent vaccines, which renders t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00917-19
更新日期:2020-03-23 00:00:00
abstract::Helicobacter pylori strains frequently express Lewis X (Le(x)) and/or Le(y) on their cell surfaces as constituents of the O antigens of their lipopolysaccharide molecules. To assess the effect of Le(x) and Le(y) expression on the ability of H. pylori to colonize the mouse stomach and to adhere to epithelial cells, iso...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.3073-3079.2002
更新日期:2002-06-01 00:00:00
abstract::The theoretic basis for developing conjugate vaccines, to induce immunoglobulin G (IgG) lipopolysaccharide (LPS) antibodies for the prevention of shigellosis, has been described (J. B. Robbins, C.-Y. Chu, and R. Schneerson, Clin. Infect. Dis. 15:346-361, 1992). The O-specific polysaccharides (O-SPs) of Shigella dysent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3678-3687.1993
更新日期:1993-09-01 00:00:00
abstract::Shigella flexneri is a gram-negative bacterium that causes bacillary dysentery in humans that is characterized by an acute inflammatory response of the colon. The fate of phagocytes that are infected in vitro with virulent Shigella has been the subject of some investigation and debate. In this study we found that viru...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.504-513.2005
更新日期:2005-01-01 00:00:00
abstract::Antibodies against the class 4 outer membrane protein (OMP) from Neisseria meningitidis have been purified from sera from vaccinees immunized with the Norwegian meningococcal group B outer membrane vesicle vaccine. The human sera and purified antibodies reacted strongly with the class 4 OMP in immunoblots, whereas exp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1267-1276.1999
更新日期:1999-03-01 00:00:00
abstract::In vitro studies have shown that enterotoxigenic Escherichia coli (ETEC) strains are capable of invading cultured epithelial cells derived from the human ileum and colon. Two separate invasion loci (tia and tib) have previously been isolated from the classical ETEC strain H10407. The tia locus has been shown to direct...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6595-6601.2000
更新日期:2000-12-01 00:00:00
abstract::Optimal protective immunity against babesial infection is postulated to require both complement-fixing and opsonizing antibodies in addition to gamma interferon (IFN-gamma)-mediated macrophage activation. The rhoptry-associated protein 1 (RAP-1) of Babesia bigemina induces partial protective immunity and is a candidat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.1.155-164.1999
更新日期:1999-01-01 00:00:00
abstract::Biological properties of two strains of simian virus 40 (SV40) from brains of two patients with progressive multifocal leukoencephalopathy (PML) have been compared to those of a standard laboratory strain of SV40. Infectivity of both SV40-PML viruses was resistant to treatment with chloroform, low pH, and 50 C for 120...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.173-179.1974
更新日期:1974-07-01 00:00:00
abstract::Actinomyces viscosus ATCC 15987 was examined for the presence of cell-associated levan by absorption of myeloma proteins with antilevan activity and direct immunofluorescence. Levan was not detectable on the surface of glucose-grown A. viscosus, but after a brief incubation of these cells with 5% sucrose, they were en...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.2.711-719.1978
更新日期:1978-02-01 00:00:00
abstract::Corynebacterium renale type I (strain 115), 1.7 X 10(7) to 4.5 X 10(7) organisms, introduced intravenously into mice disappeared from the blood less than 24 h after inoculation and did not produce pyelonephritis. The same strain, 1 X 10(7) to 5 X 10(7) organisms, inoculated into the urinary bladder of mice was not rec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.263-267.1977
更新日期:1977-04-01 00:00:00
abstract::The immunogenicity of the two major group 19 pneumococcal polysaccharides, types 19F and 19A, was studied in human volunteers. Antibody responses after vaccination with either 50 micrograms of type 19F polysaccharide, 50 micrograms of type 19A polysaccharide, or 25 micrograms of both polysaccharides premixed in a sing...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章
doi:10.1128/IAI.36.3.1261-1262.1982
更新日期:1982-06-01 00:00:00
abstract::Exoenzyme Y (ExoY) is a type III secretion system effector found in 90% of the Pseudomonas aeruginosa isolates. Although it is known that ExoY is a soluble nucleotidyl cyclase that increases the cytoplasmic levels of nucleoside 3',5'-cyclic monophosphates (cNMPs) to mediate endothelial Tau phosphorylation and permeabi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00239-17
更新日期:2017-08-18 00:00:00
abstract::The effect of human-specific antibody on surface membrane antigens of trypomastigotes of Trypanosoma cruzi was studied in vitro by using immunofluorescence methods. Immune sera induced aggregation of surface antigens in trypomastigotes to form polar cell caps. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.2.567-569.1978
更新日期:1978-05-01 00:00:00
abstract::Serotypes A and B of the relapsing fever spirochete Borrelia turicatae produce different disease manifestations in infected mice. Whereas serotype B causes more severe arthritis and reaches higher densities in the blood of mice than serotype A, serotype A invades the central nervous system earlier than serotype B duri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4637-4645.1999
更新日期:1999-09-01 00:00:00
abstract::Antibodies induced in sera and saliva of rats and rabbits immunized with ribosomal preparations from Streptococcus mutans 6715 inhibited transport of glucose by the phosphotransferase system by greater than 60%, acid production from sucrose by greater than 95%, and growth of the homologous S. mutans by greater than 59...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.286-289.1984
更新日期:1984-07-01 00:00:00
abstract::Toll-like receptors (TLRs) are a group of highly conserved molecules that initiate the innate immune response to pathogens by recognizing structural motifs expressed by microbes. We have identified a novel TLR, TLR15, by bioinformatic analysis of the chicken genome, which is distinct from any known vertebrate TLR and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.3.1692-1698.2006
更新日期:2006-03-01 00:00:00
abstract::Oophorectomized, estrogen-treated rats were immunized by the intravaginal or intranasal route with a mannoprotein extract (MP) or secreted aspartyl proteinases (Sap) of Candida albicans, with or without cholera toxin as a mucosal adjuvant. Both routes of immunization were equally effective in (i) inducing anti-MP and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2725-2729.2002
更新日期:2002-05-01 00:00:00