Helicobacter pylori does not require Lewis X or Lewis Y expression to colonize C3H/HeJ mice.

Abstract:

:Helicobacter pylori strains frequently express Lewis X (Le(x)) and/or Le(y) on their cell surfaces as constituents of the O antigens of their lipopolysaccharide molecules. To assess the effect of Le(x) and Le(y) expression on the ability of H. pylori to colonize the mouse stomach and to adhere to epithelial cells, isogenic mutants were created in which fucT1 alone or fucT1 and fucT2, which encode the fucosyl transferases necessary for Le(x) and Le(y) expression, were deleted. C3H/HeJ mice were experimentally challenged with either wild-type 26695 H. pylori or its isogenic mutants. All strains, whether passaged in the laboratory or recovered after mouse passage, colonized the mice well and without consistent differences. During colonization by the mutants, there was no reversion to wild type. Similarly, adherence to AGS and KatoIII cells was unaffected by the mutations. Together, these findings indicate that Le expression is not necessary for mouse gastric colonization or for H. pylori adherence to epithelial cells.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Takata T,El-Omar E,Camorlinga M,Thompson SA,Minohara Y,Ernst PB,Blaser MJ

doi

10.1128/iai.70.6.3073-3079.2002

keywords:

subject

Has Abstract

pub_date

2002-06-01 00:00:00

pages

3073-9

issue

6

eissn

0019-9567

issn

1098-5522

journal_volume

70

pub_type

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