Abstract:
:Helicobacter pylori strains frequently express Lewis X (Le(x)) and/or Le(y) on their cell surfaces as constituents of the O antigens of their lipopolysaccharide molecules. To assess the effect of Le(x) and Le(y) expression on the ability of H. pylori to colonize the mouse stomach and to adhere to epithelial cells, isogenic mutants were created in which fucT1 alone or fucT1 and fucT2, which encode the fucosyl transferases necessary for Le(x) and Le(y) expression, were deleted. C3H/HeJ mice were experimentally challenged with either wild-type 26695 H. pylori or its isogenic mutants. All strains, whether passaged in the laboratory or recovered after mouse passage, colonized the mice well and without consistent differences. During colonization by the mutants, there was no reversion to wild type. Similarly, adherence to AGS and KatoIII cells was unaffected by the mutations. Together, these findings indicate that Le expression is not necessary for mouse gastric colonization or for H. pylori adherence to epithelial cells.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Takata T,El-Omar E,Camorlinga M,Thompson SA,Minohara Y,Ernst PB,Blaser MJdoi
10.1128/iai.70.6.3073-3079.2002keywords:
subject
Has Abstractpub_date
2002-06-01 00:00:00pages
3073-9issue
6eissn
0019-9567issn
1098-5522journal_volume
70pub_type
杂志文章abstract::Salmonella infections continue to cause substantial morbidity and mortality throughout the world. However, recent discoveries and new paradigms promise to lead to novel strategies to diagnose, treat, and prevent Salmonella infections. This review provides an update of the Salmonella field based on oral presentations g...
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更新日期:1987-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1974-11-01 00:00:00
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更新日期:1978-09-01 00:00:00
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