An Arcanobacterium (Actinomyces) pyogenes mutant deficient in production of the pore-forming cytolysin pyolysin has reduced virulence.

Abstract:

:Pyolysin (PLO), the hemolytic exotoxin expressed by Arcanobacterium (Actinomyces) pyogenes, is a member of the thiol-activated cytolysin family of bacterial toxins. Insertional inactivation of the plo gene results in loss of expression of PLO with a concomitant loss in hemolytic activity. The plo mutant, PLO-1, has an approximately 1. 8-log10 reduction in the 50% infectious dose compared to that for wild-type A. pyogenes in a mouse intraperitoneal infection model. Studies involving cochallenge of wild-type and PLO-1 bacteria resulted in recovery of similar numbers of both strains, suggesting that PLO production is required for survival in vivo. Recombinant, His-tagged PLO (His-PLO) is cytotoxic for mouse peritoneal macrophages and J774 cells in a dose-dependent manner. Protection against challenge with A. pyogenes could be afforded by vaccination with formalin-inactivated His-PLO, suggesting that PLO is a host-protective antigen, as well as a virulence determinant.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Jost BH,Songer JG,Billington SJ

keywords:

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

1723-8

issue

4

eissn

0019-9567

issn

1098-5522

journal_volume

67

pub_type

杂志文章
  • Endoplasmic Reticulum Tubule Protein Reticulon 4 Associates with the Legionella pneumophila Vacuole and with Translocated Substrate Ceg9.

    abstract::Intracellular growth of Legionella pneumophila occurs in a replication vacuole constructed by host proteins that regulate vesicular traffic from the host endoplasmic reticulum (ER). This process is promoted by a combination of approximately 300 Icm/Dot translocated substrates (IDTS). One of these proteins, Ceg9, was p...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00507-15

    authors: Haenssler E,Ramabhadran V,Murphy CS,Heidtman MI,Isberg RR

    更新日期:2015-09-01 00:00:00

  • Digital Design of a Universal Rat Intraoral Device for Therapeutic Evaluation of a Topical Formulation against Candida-Associated Denture Stomatitis.

    abstract::Candida-associated denture stomatitis (DS) is a persistent and chronic oral infection of the denture-bearing palatal mucosa. DS stems from the ability of the fungal opportunistic pathogen Candida albicans to adhere to denture material and invade palatal tissue. Although DS is the most prevalent form of oral candidiasi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00617-19

    authors: Sultan AS,Rizk AM,Vila T,Ji Y,Masri R,Jabra-Rizk MA

    更新日期:2019-11-18 00:00:00

  • Trypanosoma cruzi-induced immunosuppression: selective triggering of CD4+ T-cell death by the T-cell receptor-CD3 pathway and not by the CD69 or Ly-6 activation pathway.

    abstract::In a model of experimental Chagas' disease induced with metacyclic forms of Trypanosoma cruzi, CD4+ but not CD8+ T cells undergo T-cell receptor (TCR)-CD3-mediated activation-induced cell death (AICD) in vitro. CD4+ T cells from T. cruzi-infected mice also developed unresponsiveness in proliferative responses to TCR-C...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.64.5.1559-1564.1996

    authors: Lopes MF,DosReis GA

    更新日期:1996-05-01 00:00:00

  • Local antibody production against the murine toxin of Yersinia pestis in a golf ball-induced granuloma.

    abstract::The method of Garra and Baygorria, for localized antibody production, has been adapted for obtaining high-titered and monospecific antibodies against the murine toxin of Yersinia pestis. A hollow, perforated plastic golf ball surgically implanted under the skin of rabbits induced the formation of a granuloma. When the...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.10.3.458-463.1974

    authors: Hillam RP,Tengerdy RP,Brown GL

    更新日期:1974-09-01 00:00:00

  • B-cell responses to pregnancy-restricted and -unrestricted Plasmodium falciparum erythrocyte membrane protein 1 antigens in Ghanaian women naturally exposed to malaria parasites.

    abstract::Protective immunity to Plasmodium falciparum malaria acquired after natural exposure is largely antibody mediated. IgG-specific P. falciparum EMP1 (PfEMP1) proteins on the infected erythrocyte surface are particularly important. The transient antibody responses and the slowly acquired protective immunity probably refl...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01514-13

    authors: Ampomah P,Stevenson L,Ofori MF,Barfod L,Hviid L

    更新日期:2014-05-01 00:00:00

  • espC pathogenicity island of enteropathogenic Escherichia coli encodes an enterotoxin.

    abstract::At least five proteins are secreted extracellularly by enteropathogenic Escherichia coli (EPEC), a leading cause of infant diarrhea in developing countries. However only one, EspC, is known to be secreted independently of the type III secretion apparatus encoded by genes located within the 35.6-kb locus of enterocyte ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.1.315-324.2001

    authors: Mellies JL,Navarro-Garcia F,Okeke I,Frederickson J,Nataro JP,Kaper JB

    更新日期:2001-01-01 00:00:00

  • Plasmodium chabaudi-infected erythrocytes adhere to CD36 and bind to microvascular endothelial cells in an organ-specific way.

    abstract::Adherence of erythrocytes infected with Plasmodium falciparum to microvascular endothelial cells (sequestration) is considered to play an important role in parasite virulence and pathogenesis. However, the real importance of sequestration for infection and disease has never been fully assessed. The absence of an appro...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.7.4135-4144.2000

    authors: Mota MM,Jarra W,Hirst E,Patnaik PK,Holder AA

    更新日期:2000-07-01 00:00:00

  • Antibody-mediated protection against infection with Helicobacter pylori in a suckling mouse model of passive immunity.

    abstract::Studies of active immunization against Helicobacter pylori indicate that antibodies play a minor role in immunity. There is also evidence, however, that the translocation of antibodies in the stomach may be insufficient to achieve functional antibody levels in the gastric lumen. We have used a suckling mouse model of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00547-09

    authors: Gorrell RJ,Robins-Browne RM

    更新日期:2009-11-01 00:00:00

  • Differences in outer membrane proteins of the lymphogranuloma venereum and trachoma biovars of Chlamydia trachomatis.

    abstract::The lymphogranuloma venereum (LGV) and trachoma biovars of Chlamydia trachomatis exhibit differences in biological properties both in vivo and in vitro. To identify analogous biochemical differences, we studied the molecular charges of chlamydial outer membrane proteins (OMPs) by means of isoelectric focusing and none...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.50.2.488-494.1985

    authors: Batteiger BE,Newhall WJ 5th,Jones RB

    更新日期:1985-11-01 00:00:00

  • Low Levels of T Cell Exhaustion in Tuberculous Lung Granulomas.

    abstract::The hallmarks of pulmonary Mycobacterium tuberculosis infection are lung granulomas. These organized structures are composed of host immune cells whose purpose is to contain or clear infection, creating a complex hub of immune and bacterial cell activity, as well as limiting pathology in the lungs. Yet, given cellular...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00426-18

    authors: Wong EA,Joslyn L,Grant NL,Klein E,Lin PL,Kirschner DE,Flynn JL

    更新日期:2018-08-22 00:00:00

  • Protective and immunochemical activities of monoclonal antibodies reactive with the Bacillus anthracis polypeptide capsule.

    abstract::Bacillus anthracis is surrounded by a polypeptide capsule composed of poly-gamma-d-glutamic acid (gammaDPGA). In a previous study, we reported that a monoclonal antibody (MAb F26G3) reactive with the capsular polypeptide is protective in a murine model of pulmonary anthrax. The present study examined a library of six ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01133-06

    authors: Kozel TR,Thorkildson P,Brandt S,Welch WH,Lovchik JA,AuCoin DP,Vilai J,Lyons CR

    更新日期:2007-01-01 00:00:00

  • Transmission-Blocking Vaccines: Old Friends and New Prospects.

    abstract::In the progression of the life cycle of Plasmodium falciparum, a small proportion of asexual parasites differentiate into male or female sexual forms called gametocytes. Just like their asexual counterparts, gametocytes are contained within the infected host's erythrocytes (RBCs). However, unlike their asexual partner...

    journal_title:Infection and immunity

    pub_type: 杂志文章,评审

    doi:10.1128/IAI.00775-18

    authors: Acquah FK,Adjah J,Williamson KC,Amoah LE

    更新日期:2019-05-21 00:00:00

  • T cell-mediated immunity in the lung: a Cryptococcus neoformans pulmonary infection model using SCID and athymic nude mice.

    abstract::T cells are important in systemic anticryptococcal defenses, but a role in controlling an initial pulmonary infection has not been demonstrated. A murine model with intratracheal inoculation was developed to study the acquisition and expression of pulmonary T cell-mediated immunity against Cryptococcus neoformans. Inf...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.4.1423-1433.1991

    authors: Huffnagle GB,Yates JL,Lipscomb MF

    更新日期:1991-04-01 00:00:00

  • Skin lesions produced by Staphylococcus aureus exfoliatin in hairless mice.

    abstract::Exfoliatin caused skin loss in hairless mutant mice only while they lacked hair, but older mice were more resistant to this effect than neonates. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.6.5.877-879.1972

    authors: Kapral FA,Miller MM

    更新日期:1972-11-01 00:00:00

  • Heparin inhibits phagocytosis by polymorphonuclear leukocytes.

    abstract::Phagocytosis of unopsonized Salmonella typhimurium 395, MR-10, opsonized Salmonella typhimurium 395 MS, and Staphylococcus epidermidis by rabbit polymorphonuclear leukocytes was inhibited by heparin at concentrations as low as 0.5 U/ml. Inhibition was dose dependent and nearly complete at 20 U/ml. Provided that hepari...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.32.1.295-299.1981

    authors: Victor M,Weiss J,Elsbach P

    更新日期:1981-04-01 00:00:00

  • Cell-associated hemagglutinin-deficient mutant of Vibrio cholerae.

    abstract::Cell-associated hemagglutinin-negative mutants were derived from cholera enterotoxin-negative Vibrio cholerae JBK70 by Tn5 mutagenesis. One of the mutants identified, SB001, was characterized in greater detail. Its ability to colonize ilea of adult rabbits was determined by feeding approximately 10(8) V. cholerae to e...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.55.4.942-946.1987

    authors: Finn TM,Reiser J,Germanier R,Cryz SJ Jr

    更新日期:1987-04-01 00:00:00

  • Characterization of monoclonal antibodies against alpha-hemolysin of Escherichia coli.

    abstract::Monoclonal antibodies (MAbs) were raised against native and denatured alpha-hemolysin (HlyA) of Escherichia coli. Binding of the MAbs to native, denatured, and erythrocyte-complexed active wild-type hemolysin and mutant derivatives was tested. All 15 MAbs analyzed bound to native hemolysin, even when the toxin was com...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.5.1846-1852.1991

    authors: Oropeza-Wekerle RL,Kern P,Sun D,Muller S,Briand JP,Goebel W

    更新日期:1991-05-01 00:00:00

  • Impairment of melibiose utilization in Streptococcus mutans serotype c gtfA mutants.

    abstract::The Streptococcus mutans serotype c gtfA gene encodes a 55-kilodalton sucrose-hydrolyzing enzyme. Analysis of S. mutans gtfA mutants revealed that the mutant strains were specifically impaired in the ability to use melibiose as a sole carbon source. S. mutans gtfA mutant strains synthesized less alpha-galactosidase ac...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.3.992-995.1989

    authors: Barletta RG,Curtiss R 3rd

    更新日期:1989-03-01 00:00:00

  • Contribution of Veillonella parvula to Pseudomonas aeruginosa-mediated pathogenicity in a murine tumor model system.

    abstract::The recent finding that high numbers of strict anaerobes are present in the respiratory tract of cystic fibrosis (CF) patients has drawn attention to the pathogenic contribution of the CF microbiome to airway disease. In this study, we investigated the specific interactions of the most dominant bacterial CF pathogen, ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.02234-14

    authors: Pustelny C,Komor U,Pawar V,Lorenz A,Bielecka A,Moter A,Gocht B,Eckweiler D,Müsken M,Grothe C,Lünsdorf H,Weiss S,Häussler S

    更新日期:2015-01-01 00:00:00

  • CXCL16 regulates cell-mediated immunity to Salmonella enterica serovar Enteritidis via promotion of gamma interferon production.

    abstract::CXCL16 is a recently discovered multifaceted chemokine that has been shown not only to recruit activated T lymphocytes but also to play a direct role in the binding and phagocytosis of bacteria by professional antigen-presenting cells. In this study, we investigated the role of CXCL16 in vivo in the regulation of the ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01065-06

    authors: Fahy OL,Townley SL,McColl SR

    更新日期:2006-12-01 00:00:00

  • Synthesis, characterization, and some immunological properties of conjugates composed of the detoxified lipopolysaccharide of Vibrio cholerae O1 serotype Inaba bound to cholera toxin.

    abstract::Protection against cholera has been correlated with the level of serum vibriocidal antibodies. The specificity of these vibriocidal antibodies was mostly to the lipopolysaccharide (LPS). We synthesized conjugates of detoxified LPS with cholera toxin (CT) and other proteins in order to elicit serum LPS antibodies with ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.8.3201-3208.1992

    authors: Gupta RK,Szu SC,Finkelstein RA,Robbins JB

    更新日期:1992-08-01 00:00:00

  • MyD88, but not toll-like receptors 4 and 2, is required for efficient clearance of Brucella abortus.

    abstract::It is not clear how the host initially recognizes and responds to infection by gram-negative pathogenic Brucella spp. It was previously shown (D. S. Weiss, B. Raupach, K. Takeda, S. Akira, and A. Zychlinsky, J. Immunol. 172:4463-4469, 2004) that the early macrophage response against gram-negative bacteria is mediated ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.8.5137-5143.2005

    authors: Weiss DS,Takeda K,Akira S,Zychlinsky A,Moreno E

    更新日期:2005-08-01 00:00:00

  • Virulence properties and attaching-effacing activity of Escherichia coli O45 from swine postweaning diarrhea.

    abstract::Escherichia coli O45 isolates associated with swine postweaning diarrhea in Québec were characterized with respect to virulence determinants genetically and investigated for their attaching and effacing (A/E) activities by experimental inoculation of gnotobiotic piglets and by the HEp-2 cell adherence assay. All of 32...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.10.4153-4159.1994

    authors: Zhu C,Harel J,Jacques M,Desautels C,Donnenberg MS,Beaudry M,Fairbrother JM

    更新日期:1994-10-01 00:00:00

  • Initial serum antibody titer to Porphyromonas gingivalis influences development of antibody avidity and success of therapy for chronic periodontitis.

    abstract::This study assessed the effect of periodontal therapy on specific serum antibody concentration, expressed as titer, and antibody binding strength, expressed as relative avidity. The immune responses to Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans were investigated. Antibody titer was assayed by en...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.9.3411-3416.1995

    authors: Mooney J,Adonogianaki E,Riggio MP,Takahashi K,Haerian A,Kinane DF

    更新日期:1995-09-01 00:00:00

  • The Inflammatory response induced by aspartic proteases of Candida albicans is independent of proteolytic activity.

    abstract::The secretion of aspartic proteases (Saps) has long been recognized as a virulence-associated trait of the pathogenic yeast Candida albicans. In this study, we report that different recombinant Saps, including Sap1, Sap2, Sap3, and Sap6, have differing abilities to induce secretion of proinflammatory cytokines by huma...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00789-10

    authors: Pietrella D,Rachini A,Pandey N,Schild L,Netea M,Bistoni F,Hube B,Vecchiarelli A

    更新日期:2010-11-01 00:00:00

  • The role of nitric oxide in experimental murine sepsis due to pyrogenic exotoxin A-producing Streptococcus pyogenes.

    abstract::Nitric oxide (NO) produced by inducible NO synthase (iNOS) mediates hypotension in endotoxemia. In this study, NO induction by a toxin-producing Streptococcus pyogenes isolate, H250, and by recombinant streptococcal pyrogenic exotoxin A (rSPEA) has been examined, both in vitro and in vivo. Streptococcal supernatants, ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.5.1767-1772.1997

    authors: Sriskandan S,Moyes D,Buttery LK,Wilkinson J,Evans TJ,Polak J,Cohen J

    更新日期:1997-05-01 00:00:00

  • Probable L-forms of Nocardia asteroides induced in cultured mouse peritoneal macrophages.

    abstract::Mouse peritoneal macrophages were infected with varying numbers of Nocardia asteroides 10905, and the fate of the ingested organisms was determined by viable plate count (VPC), light microscopy (LM), immunofluorescent microscopy (IM), and electron microscopy (EM). The results obtained with these methods differed. VPC ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.9.3.576-590.1974

    authors: Bourgeois L,Beaman BL

    更新日期:1974-03-01 00:00:00

  • Replication of dengue and junin viruses in cultured rabbit and human endothelial cells.

    abstract::The flavivirus dengue and the arenavirus Junin are both associated with a hemorrhagic shock syndrome in man. We have demonstrated the replication of these viruses in vitro in both rabbit and human endothelial cells by viral titers and immunofluorescent antibody studies. Rabbit endothelium established in continuous cul...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.20.3.776-781.1978

    authors: Andrews BS,Theofilopoulos AN,Peters CJ,Loskutoff DJ,Brandt WE,Dixon FJ

    更新日期:1978-06-01 00:00:00

  • Cultivation and partial characterization of spiroplasmas in cell cultures.

    abstract::Spiroplasmas were propagated in the Drosophila melanogaster cell line Dm-1. Spiroplasma citri and unidentified strains (corn shunt organism, 277F [tick isolate], powder puff, BNR-1, honey bee, and OBMG) grew to 10(8) to 10(9) colony-forming units per ml and could be passaged. Cytopathic effect (CPE) varied with the in...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.35.1.296-304.1982

    authors: Steiner T,McGarrity GJ,Phillips DM

    更新日期:1982-01-01 00:00:00

  • Effect of immunosuppression on the genetic resistance of A2G mice to neurovirulent influenza virus.

    abstract::A2G mice are genetically resistant to lethal infection with neurotropic and pneumotropic influenza viruses. A possible immunological explanation for this resistance was sought by assessing the effect of cyclophosphamide and X irradiation immunosuppression on the infection of A2G mice with lethal doses of neurovirulent...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.11.3.576-587.1975

    authors: Fiske RA,Klein PA

    更新日期:1975-03-01 00:00:00