Abstract:
:Pyolysin (PLO), the hemolytic exotoxin expressed by Arcanobacterium (Actinomyces) pyogenes, is a member of the thiol-activated cytolysin family of bacterial toxins. Insertional inactivation of the plo gene results in loss of expression of PLO with a concomitant loss in hemolytic activity. The plo mutant, PLO-1, has an approximately 1. 8-log10 reduction in the 50% infectious dose compared to that for wild-type A. pyogenes in a mouse intraperitoneal infection model. Studies involving cochallenge of wild-type and PLO-1 bacteria resulted in recovery of similar numbers of both strains, suggesting that PLO production is required for survival in vivo. Recombinant, His-tagged PLO (His-PLO) is cytotoxic for mouse peritoneal macrophages and J774 cells in a dose-dependent manner. Protection against challenge with A. pyogenes could be afforded by vaccination with formalin-inactivated His-PLO, suggesting that PLO is a host-protective antigen, as well as a virulence determinant.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Jost BH,Songer JG,Billington SJkeywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
1723-8issue
4eissn
0019-9567issn
1098-5522journal_volume
67pub_type
杂志文章abstract::Intracellular growth of Legionella pneumophila occurs in a replication vacuole constructed by host proteins that regulate vesicular traffic from the host endoplasmic reticulum (ER). This process is promoted by a combination of approximately 300 Icm/Dot translocated substrates (IDTS). One of these proteins, Ceg9, was p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00507-15
更新日期:2015-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00617-19
更新日期:2019-11-18 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.5.1559-1564.1996
更新日期:1996-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.3.458-463.1974
更新日期:1974-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01514-13
更新日期:2014-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.315-324.2001
更新日期:2001-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4135-4144.2000
更新日期:2000-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00547-09
更新日期:2009-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.488-494.1985
更新日期:1985-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00426-18
更新日期:2018-08-22 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01133-06
更新日期:2007-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.00775-18
更新日期:2019-05-21 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1423-1433.1991
更新日期:1991-04-01 00:00:00
abstract::Exfoliatin caused skin loss in hairless mutant mice only while they lacked hair, but older mice were more resistant to this effect than neonates. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.5.877-879.1972
更新日期:1972-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.295-299.1981
更新日期:1981-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.4.942-946.1987
更新日期:1987-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.5.1846-1852.1991
更新日期:1991-05-01 00:00:00
abstract::The Streptococcus mutans serotype c gtfA gene encodes a 55-kilodalton sucrose-hydrolyzing enzyme. Analysis of S. mutans gtfA mutants revealed that the mutant strains were specifically impaired in the ability to use melibiose as a sole carbon source. S. mutans gtfA mutant strains synthesized less alpha-galactosidase ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.992-995.1989
更新日期:1989-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02234-14
更新日期:2015-01-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.01065-06
更新日期:2006-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3201-3208.1992
更新日期:1992-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.5137-5143.2005
更新日期:2005-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4153-4159.1994
更新日期:1994-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3411-3416.1995
更新日期:1995-09-01 00:00:00
abstract::The secretion of aspartic proteases (Saps) has long been recognized as a virulence-associated trait of the pathogenic yeast Candida albicans. In this study, we report that different recombinant Saps, including Sap1, Sap2, Sap3, and Sap6, have differing abilities to induce secretion of proinflammatory cytokines by huma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00789-10
更新日期:2010-11-01 00:00:00
abstract::Nitric oxide (NO) produced by inducible NO synthase (iNOS) mediates hypotension in endotoxemia. In this study, NO induction by a toxin-producing Streptococcus pyogenes isolate, H250, and by recombinant streptococcal pyrogenic exotoxin A (rSPEA) has been examined, both in vitro and in vivo. Streptococcal supernatants, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.5.1767-1772.1997
更新日期:1997-05-01 00:00:00
abstract::Mouse peritoneal macrophages were infected with varying numbers of Nocardia asteroides 10905, and the fate of the ingested organisms was determined by viable plate count (VPC), light microscopy (LM), immunofluorescent microscopy (IM), and electron microscopy (EM). The results obtained with these methods differed. VPC ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.3.576-590.1974
更新日期:1974-03-01 00:00:00
abstract::The flavivirus dengue and the arenavirus Junin are both associated with a hemorrhagic shock syndrome in man. We have demonstrated the replication of these viruses in vitro in both rabbit and human endothelial cells by viral titers and immunofluorescent antibody studies. Rabbit endothelium established in continuous cul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.776-781.1978
更新日期:1978-06-01 00:00:00
abstract::Spiroplasmas were propagated in the Drosophila melanogaster cell line Dm-1. Spiroplasma citri and unidentified strains (corn shunt organism, 277F [tick isolate], powder puff, BNR-1, honey bee, and OBMG) grew to 10(8) to 10(9) colony-forming units per ml and could be passaged. Cytopathic effect (CPE) varied with the in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.296-304.1982
更新日期:1982-01-01 00:00:00
abstract::A2G mice are genetically resistant to lethal infection with neurotropic and pneumotropic influenza viruses. A possible immunological explanation for this resistance was sought by assessing the effect of cyclophosphamide and X irradiation immunosuppression on the infection of A2G mice with lethal doses of neurovirulent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.3.576-587.1975
更新日期:1975-03-01 00:00:00